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The map label for this gene is ybiK [C]

Identifier: 84623317

GI number: 84623317

Start: 1815757

End: 1816821

Strand: Direct

Name: ybiK [C]

Synonym: XOO_1660

Alternate gene names: 84623317

Gene position: 1815757-1816821 (Clockwise)

Preceding gene: 84623316

Following gene: 84623318

Centisome position: 36.75

GC content: 64.51

Gene sequence:

>1065_bases
ATGATTCAACGTCGTCACTTCCTCAAGAGCGCCACGCTGGGCGCGATCGCGTCCAGCGTGGCCGGCTGGGGCGCACGCGC
GCAGGCGGCGGTGGGCGCCACGTCGGGCGTGCGGCCGCCAGCGCTGCCGCGTGGTGCGGCACGGGTGATTTCCACCTGGG
ATTTCGGCATTGCTGCCAATCAGGAGGCGTGGAAGATTCTGAGCAGCGGTGGCATCGCGCTGGATGCGGTGGAAGCTGGC
GTGCGCGTGCCGGAAGCGGACCCGACCAATCCCACCGTCGGCCTGGGCGGTTATCCGGACCGCGACGGGCGGGTAACGCT
GGACGCCTGCATCATGGATCACCTGGGCAATTGCGGCGCGGTGGCAGCGCTGGAAGATGTGGTGCATGCCATTTCGGTGG
CGCGCGCTGTGATGGAGAAAACGCCGCACGTGATGTTGGTCGGCGATGGCGCGCGGCAGTTCGCGTTGGAGCAAGGCTTT
CCGAAAACCAAGCTGCTCACGCCGAGCTCGGAAGCGGCCTGGAAGGAGTGGTTGAAGACTTCCAGATATTTGCCGGAAGC
CAATGTGGAAAATCGCGCCTGGCGCGATGCCAAATTACCGGGAGGCAAGGATAATCACGACACCATCGGCATGCTGGCGC
TGGATACGCACGGCAATCTTTCGGGTGCGTGCACCACCAGCGGCATGGCGTGGAAAATGCACGGGCGCGTGGGCGACAGC
CCGATCATCGGCGCGGGCTTGTACGTGGACAACGAAGTGGGCGGCGCAACCTCGACCGGTGTGGGCGAAGAAGTGATCCG
GAATGTCGGCAGTTTTGCGGTGGTGGAAATGATGCGGCAGGGCAAGTCGCCCGCTGATGCGTGCCGCGAGATGGTGATGC
GGCTGATCCGTCGCAAGCCGGAGCTGACGCGCACCTTGCAGGTCGGGTTTCTGGCGATGAACAAGCGCGGTGAAGTGGGG
GCGTTCGCGATTCAGAAGGGCTTTAGCTACGCGGTGTGCGATGCGCAGCGGCAGGATCTGCTGGTGCCTGGTGAGAGCCA
TTACACCAGCGAGCCGGCAGCATGA

Upstream 100 bases:

>100_bases
ACACCGCCACAACATGGTTTATCCGCACGCGACGCTGCCGCATGATGGAAGGCGGCTGCCGCACTGCGCGGCCGTTCGTT
TCCATCATGGAGTCGCCCGC

Downstream 100 bases:

>100_bases
GTGCATTAGGTCTTGAAGTGGCCGCCGATTCGGTGGCCTCGGCGTTGGCCGCGCAAGAAGGCGGAGCGATGCGCGTGGAG
CTGTGCGGTGGGCTGGATGG

Product: asparaginase

Products: NA

Alternate protein names: Aspartylglucosaminidase; AGA; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MIQRRHFLKSATLGAIASSVAGWGARAQAAVGATSGVRPPALPRGAARVISTWDFGIAANQEAWKILSSGGIALDAVEAG
VRVPEADPTNPTVGLGGYPDRDGRVTLDACIMDHLGNCGAVAALEDVVHAISVARAVMEKTPHVMLVGDGARQFALEQGF
PKTKLLTPSSEAAWKEWLKTSRYLPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
PIIGAGLYVDNEVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREMVMRLIRRKPELTRTLQVGFLAMNKRGEVG
AFAIQKGFSYAVCDAQRQDLLVPGESHYTSEPAA

Sequences:

>Translated_354_residues
MIQRRHFLKSATLGAIASSVAGWGARAQAAVGATSGVRPPALPRGAARVISTWDFGIAANQEAWKILSSGGIALDAVEAG
VRVPEADPTNPTVGLGGYPDRDGRVTLDACIMDHLGNCGAVAALEDVVHAISVARAVMEKTPHVMLVGDGARQFALEQGF
PKTKLLTPSSEAAWKEWLKTSRYLPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
PIIGAGLYVDNEVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREMVMRLIRRKPELTRTLQVGFLAMNKRGEVG
AFAIQKGFSYAVCDAQRQDLLVPGESHYTSEPAA
>Mature_354_residues
MIQRRHFLKSATLGAIASSVAGWGARAQAAVGATSGVRPPALPRGAARVISTWDFGIAANQEAWKILSSGGIALDAVEAG
VRVPEADPTNPTVGLGGYPDRDGRVTLDACIMDHLGNCGAVAALEDVVHAISVARAVMEKTPHVMLVGDGARQFALEQGF
PKTKLLTPSSEAAWKEWLKTSRYLPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
PIIGAGLYVDNEVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREMVMRLIRRKPELTRTLQVGFLAMNKRGEVG
AFAIQKGFSYAVCDAQRQDLLVPGESHYTSEPAA

Specific function: Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N- (R1) and C- (R2) terminus [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI285002251, Length=319, Percent_Identity=36.3636363636364, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI285002253, Length=319, Percent_Identity=34.4827586206897, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI145275202, Length=229, Percent_Identity=37.5545851528384, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI145275200, Length=229, Percent_Identity=37.5545851528384, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI98991769, Length=235, Percent_Identity=28.936170212766, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1787050, Length=259, Percent_Identity=35.9073359073359, Blast_Score=131, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17569239, Length=302, Percent_Identity=34.4370860927152, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI221330102, Length=301, Percent_Identity=40.1993355481728, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24652135, Length=301, Percent_Identity=40.1993355481728, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24655553, Length=300, Percent_Identity=34.6666666666667, Blast_Score=151, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24657358, Length=291, Percent_Identity=33.3333333333333, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI18921183, Length=252, Percent_Identity=35.7142857142857, Blast_Score=119, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.5.1.26 [H]

Molecular weight: Translated: 37500; Mature: 37500

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQRRHFLKSATLGAIASSVAGWGARAQAAVGATSGVRPPALPRGAARVISTWDFGIAAN
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
QEAWKILSSGGIALDAVEAGVRVPEADPTNPTVGLGGYPDRDGRVTLDACIMDHLGNCGA
HHHHHHHHCCCEEEEHHHHCCCCCCCCCCCCEECCCCCCCCCCCEEHHHHHHHHCCCCCH
VAALEDVVHAISVARAVMEKTPHVMLVGDGARQFALEQGFPKTKLLTPSSEAAWKEWLKT
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
SRYLPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
HCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEECCCEEEEECCCCCCC
PIIGAGLYVDNEVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREMVMRLIRRKP
CEEEEEEEEECCCCCCCCCCCHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
ELTRTLQVGFLAMNKRGEVGAFAIQKGFSYAVCDAQRQDLLVPGESHYTSEPAA
HHHHHHHHHEEEECCCCCCCHHHHHCCCCEEEECCCCCCEECCCCCCCCCCCCC
>Mature Secondary Structure
MIQRRHFLKSATLGAIASSVAGWGARAQAAVGATSGVRPPALPRGAARVISTWDFGIAAN
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
QEAWKILSSGGIALDAVEAGVRVPEADPTNPTVGLGGYPDRDGRVTLDACIMDHLGNCGA
HHHHHHHHCCCEEEEHHHHCCCCCCCCCCCCEECCCCCCCCCCCEEHHHHHHHHCCCCCH
VAALEDVVHAISVARAVMEKTPHVMLVGDGARQFALEQGFPKTKLLTPSSEAAWKEWLKT
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
SRYLPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
HCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEECCCEEEEECCCCCCC
PIIGAGLYVDNEVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREMVMRLIRRKP
CEEEEEEEEECCCCCCCCCCCHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
ELTRTLQVGFLAMNKRGEVGAFAIQKGFSYAVCDAQRQDLLVPGESHYTSEPAA
HHHHHHHHHEEEECCCCCCCHHHHHCCCCEEEECCCCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7840643; 8250923; 9541410 [H]