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The map label for this gene is nadE [H]

Identifier: 84623136

GI number: 84623136

Start: 1619815

End: 1621470

Strand: Direct

Name: nadE [H]

Synonym: XOO_1479

Alternate gene names: 84623136

Gene position: 1619815-1621470 (Clockwise)

Preceding gene: 84623135

Following gene: 84623138

Centisome position: 32.79

GC content: 66.36

Gene sequence:

>1656_bases
ATGGAGTCAGCAGTGACAGCACACACCCTCCGCATCGCCATGGCCCAGTTCGACTTCCCGGTCGGCGGCGTGACGCAGAA
TACCGACCGCATCATCGAGTACATCGCAGCGGCGCGCGATGAGTTCGAGGCCGACATCGTGCTGTTTCCCGAGCTGGCGA
TCAGCGGTTATCCGCCGGAAGATCTGCTGCTGCGTCCCGGGTTTCTTGCGCACTGCGAACAGGCGCTGGCACGGATTGCG
GCTGCCACGCGCGGTATCGTGGCGGTGGTCGGCTGGCCACAGAGCGCCGGCAGCGTGGTCTATAACGCCGCCAGCGTGTT
GCGCGATGGGCGCATCGAAGCCACCTACCGAAAGCGCGAATTGCCGAACTATGCGGTGTTCGACGAGCGTCGTTATTTCG
ACGTGGACCCGGATGGCGAGAACTGCGTGGTCACGGTCAAGGGCGTGCAGGTGGGCGTGGTGATCTGCGAAGACCTCTGG
TTCGCCGAGCCGCTGGCCAACACCGTGAAGGCCGGCGCCGAACTGGTGCTGGTGCCCAACGCCTCGCCATACGAACGCGG
CAAGCATGCGCAACGCGATGCATTGCTGGCCGAACGCACACGCGAGAGCGGGGCAGCCATTGCCTATCTCAACGTCGTGG
GCGGGCAGGATGCACTGGTGTTCGATGGCGCCTCGGTGGTGGCCGATGGCGACGGTACCGTGCATCCGGCTGCAGCCGCC
TTCGTGGACCAGTGGCTGGTGGTGGACTACGCGGCCGGCGAGCGCAGCTTCACGCCGGTGGTATGGGTGGACGACGGCGA
CGAGAGCATGGACGCGCTGGCCTGGCGCGCGGTGGTGCGCGGGTTGCAGGACTACTGCAGCAAGAACGGCTTCAGCAAGG
TCTGGCTGGGCCTGTCCGGCGGCATCGACTCGGCGCTGGTGCTGGCGATGGCAGTGGACGCACTGGGCGGCGACAACGTC
ACCGCCGTGCGCTTGCCGTCGCGCTACACCGCCAACCTGTCCAACGATCTGGCCGACGAGCAATGCCGCGCGCTGGGCGT
GAAGCTGGAGACGGTTGCCATCGAGCCGGCCTTCGAGGGCTTGCTGGCGGCGCTGGGGCCGCTGTTCGCTGGCACCGAGC
CGGACATCACCGAGGAAAACCTGCAGTCGCGCAGCCGTGGCGTGATCCTGATGGCGCTGTCGAACAAGTTCGGCGGGCTG
GTACTGACCACCGGCAACAAGAGCGAATACGCAGTGGGCTACGCCACCATCTATGGCGACATGTGCGGTGGCTATGCGCC
GCTCAAGGACCTGTACAAGACGGAGGTCTTCGGCCTGGCCAAGTGGCGCAATACCGTGGACGGCGCGCCGGTGATTCCGC
CAGCGGTGATCTCGCGCCCGCCGTCGGCCGAGTTGCGCGACAACCAGACCGACCAGGATTCACTGCCGCCGTACGATGTG
CTCGACGCCATCCTGTACCGCTACGTCGATCAGGAGCAGTCGCGCGACGACATCGTCGCCGCCGGCTACGCCGCAGAGAC
GGTGGAGCATGTGCTGCGCCTGGTGCGGCTCAACGAATGGAAGCGCCATCAGTCCGCGCCCGGACCCAAGGTGTCGCGGC
GTGCATTCGGGCGCGAACGGCGCTATCCGATCACCAACGGGTATCGCGGCCAGTAG

Upstream 100 bases:

>100_bases
CTATCTGCCGTTCTATCGCGACGCCCCGTTTGGACGACACCGTGTATGTGCTGGCGATCCGTTCGCAGCGCGAAGCGGGT
TACCTTGAAAGCCTGTTTTG

Downstream 100 bases:

>100_bases
CCCGAATGGCTAATACGGTCAGTGGCTTGGTGCACCGGTCGCCGCTTGCGGTGGCCGGTGGTTGGGCGTCGCTGCACGCA
CCGCGCGGTCTTCGCCATCG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MESAVTAHTLRIAMAQFDFPVGGVTQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPEDLLLRPGFLAHCEQALARIA
AATRGIVAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRELPNYAVFDERRYFDVDPDGENCVVTVKGVQVGVVICEDLW
FAEPLANTVKAGAELVLVPNASPYERGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAA
FVDQWLVVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCSKNGFSKVWLGLSGGIDSALVLAMAVDALGGDNV
TAVRLPSRYTANLSNDLADEQCRALGVKLETVAIEPAFEGLLAALGPLFAGTEPDITEENLQSRSRGVILMALSNKFGGL
VLTTGNKSEYAVGYATIYGDMCGGYAPLKDLYKTEVFGLAKWRNTVDGAPVIPPAVISRPPSAELRDNQTDQDSLPPYDV
LDAILYRYVDQEQSRDDIVAAGYAAETVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRERRYPITNGYRGQ

Sequences:

>Translated_551_residues
MESAVTAHTLRIAMAQFDFPVGGVTQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPEDLLLRPGFLAHCEQALARIA
AATRGIVAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRELPNYAVFDERRYFDVDPDGENCVVTVKGVQVGVVICEDLW
FAEPLANTVKAGAELVLVPNASPYERGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAA
FVDQWLVVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCSKNGFSKVWLGLSGGIDSALVLAMAVDALGGDNV
TAVRLPSRYTANLSNDLADEQCRALGVKLETVAIEPAFEGLLAALGPLFAGTEPDITEENLQSRSRGVILMALSNKFGGL
VLTTGNKSEYAVGYATIYGDMCGGYAPLKDLYKTEVFGLAKWRNTVDGAPVIPPAVISRPPSAELRDNQTDQDSLPPYDV
LDAILYRYVDQEQSRDDIVAAGYAAETVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRERRYPITNGYRGQ
>Mature_551_residues
MESAVTAHTLRIAMAQFDFPVGGVTQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPEDLLLRPGFLAHCEQALARIA
AATRGIVAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRELPNYAVFDERRYFDVDPDGENCVVTVKGVQVGVVICEDLW
FAEPLANTVKAGAELVLVPNASPYERGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAA
FVDQWLVVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCSKNGFSKVWLGLSGGIDSALVLAMAVDALGGDNV
TAVRLPSRYTANLSNDLADEQCRALGVKLETVAIEPAFEGLLAALGPLFAGTEPDITEENLQSRSRGVILMALSNKFGGL
VLTTGNKSEYAVGYATIYGDMCGGYAPLKDLYKTEVFGLAKWRNTVDGAPVIPPAVISRPPSAELRDNQTDQDSLPPYDV
LDAILYRYVDQEQSRDDIVAAGYAAETVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRERRYPITNGYRGQ

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59678; Mature: 59678

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESAVTAHTLRIAMAQFDFPVGGVTQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPE
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHCCCCCCH
DLLLRPGFLAHCEQALARIAAATRGIVAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRE
HHEECCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEHHHHHC
LPNYAVFDERRYFDVDPDGENCVVTVKGVQVGVVICEDLWFAEPLANTVKAGAELVLVPN
CCCCEEECCCEEECCCCCCCCEEEEEECEEEEEEEECHHHHHHHHHHHHHCCCEEEEECC
ASPYERGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAA
CCHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEECCCEEEECCCCCCCHHHHH
FVDQWLVVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCSKNGFSKVWLGLSG
HHHHHEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GIDSALVLAMAVDALGGDNVTAVRLPSRYTANLSNDLADEQCRALGVKLETVAIEPAFEG
CHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEEEECHHHHH
LLAALGPLFAGTEPDITEENLQSRSRGVILMALSNKFGGLVLTTGNKSEYAVGYATIYGD
HHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEEEHH
MCGGYAPLKDLYKTEVFGLAKWRNTVDGAPVIPPAVISRPPSAELRDNQTDQDSLPPYDV
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHH
LDAILYRYVDQEQSRDDIVAAGYAAETVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRER
HHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCC
RYPITNGYRGQ
CCCCCCCCCCC
>Mature Secondary Structure
MESAVTAHTLRIAMAQFDFPVGGVTQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPE
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHCCCCCCH
DLLLRPGFLAHCEQALARIAAATRGIVAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRE
HHEECCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEHHHHHC
LPNYAVFDERRYFDVDPDGENCVVTVKGVQVGVVICEDLWFAEPLANTVKAGAELVLVPN
CCCCEEECCCEEECCCCCCCCEEEEEECEEEEEEEECHHHHHHHHHHHHHCCCEEEEECC
ASPYERGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAA
CCHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEECCCEEEECCCCCCCHHHHH
FVDQWLVVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCSKNGFSKVWLGLSG
HHHHHEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GIDSALVLAMAVDALGGDNVTAVRLPSRYTANLSNDLADEQCRALGVKLETVAIEPAFEG
CHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEEEECHHHHH
LLAALGPLFAGTEPDITEENLQSRSRGVILMALSNKFGGLVLTTGNKSEYAVGYATIYGD
HHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEEEHH
MCGGYAPLKDLYKTEVFGLAKWRNTVDGAPVIPPAVISRPPSAELRDNQTDQDSLPPYDV
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHH
LDAILYRYVDQEQSRDDIVAAGYAAETVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRER
HHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCC
RYPITNGYRGQ
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]