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The map label for this gene is pilB [H]

Identifier: 84623129

GI number: 84623129

Start: 1610537

End: 1612270

Strand: Direct

Name: pilB [H]

Synonym: XOO_1472

Alternate gene names: 84623129

Gene position: 1610537-1612270 (Clockwise)

Preceding gene: 84623128

Following gene: 84623133

Centisome position: 32.6

GC content: 61.19

Gene sequence:

>1734_bases
ATGAATTCAGTATCTACAACCAACCTCGTTGGCATCACCGGCATCGCTCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AACCGCTGCGCGGACAGCGATGGAACAGGCGGCCGCTGCCAAGGTGCCGCTGCCGCAGTGGTTTGCCGAGAACAGGCTGG
TGTCGGCGTCGCAGCTTGCGGCGGCCAATGCAGTGGAATTTGGCATGCCACTGATGGATGTGTCTGTGTTCGACGCTAAC
CAGAACGCAGTCAAGCTAGTCAGCGAGGAGTTGCTCCAGAAGTACCATGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
GTTTGTGGGAGTGAGCAACCCGACTCAGACCCGTGCACTAGATGACATCAAGTTTCATACGAATCTGGTCGTCGAGCCGA
TTCTTGTCGATGAGGACCAAATCCGTCGCACATTGGAGCAGTGGCAGGCAAGCAATGCCTCATTTGGATCATCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCCACCGGCGACGAAGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGGGCCTCGGACA
TCCATTTCGAGCCGTACGAAGACGACTACCGGGTGCGGTTGCGGATCGACGGGTTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAGCCAGCGCATCGCGGCGCGTTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAACAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGCT
GTACACGGCGCTGGGCATCCTCAACGATGAGACGCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATTCGCCTGCCTG
GCGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGTTCGTTCCTGCGACAGGATCCG
GACATCATCATGGTTGGCGAAATCCGCGACCTGGAAACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGGATCGCGCCGTACAACATCA
CCTCGTCGGTGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAATAACTGCAAGCGCAAGTCGACGTTGCCG
GAGCATGCACTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTCGCGAATATGGAGCTTTATGAAGCCGTGGGTTGCGACGA
GTGCACCGAGGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATTGCCGAGGCCGCTCAGAAGATCGGCATCCGGGACTTGCGGCAGTCGGCGTTGATGAAG
GCCTCGCAAGGCGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTGA

Upstream 100 bases:

>100_bases
CCAGTCGAGGTCAATACTGACCTGAGGATGTTGACCGAGTATAAATATGGGGCATCGTCCCGATGATCGGGAAGATGGGG
ACATCAGCGGGAATCAAACG

Downstream 100 bases:

>100_bases
GTGAAGCGTCAGTCGTAATCCTGATCGGCTGACGGTTCTTTGATCTGCATGATGCAGCTATCAAAGGTGTCCTCTAGCTG
GGCGTAATTGCATGACACTG

Product: pilus biogenesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MNSVSTTNLVGITGIARRLVQDGALEETAARTAMEQAAAAKVPLPQWFAENRLVSASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYHVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASFGSSLG
NDDEEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASQGVTSLAEINRVTKD

Sequences:

>Translated_577_residues
MNSVSTTNLVGITGIARRLVQDGALEETAARTAMEQAAAAKVPLPQWFAENRLVSASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYHVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASFGSSLG
NDDEEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASQGVTSLAEINRVTKD
>Mature_577_residues
MNSVSTTNLVGITGIARRLVQDGALEETAARTAMEQAAAAKVPLPQWFAENRLVSASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYHVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASFGSSLG
NDDEEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASQGVTSLAEINRVTKD

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=381, Percent_Identity=45.4068241469816, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI1789723, Length=425, Percent_Identity=42.3529411764706, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI87082188, Length=184, Percent_Identity=37.5, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62971; Mature: 62971

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSVSTTNLVGITGIARRLVQDGALEETAARTAMEQAAAAKVPLPQWFAENRLVSASQLA
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHH
AANAVEFGMPLMDVSVFDANQNAVKLVSEELLQKYHVLPLFKRGNRLFVGVSNPTQTRAL
HHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCCCHHHHH
DDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASFGSSLGNDDEEMGDLDVSTGDEDMGA
HHHHHHCCEEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
GGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHH
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEHHHHHHHHCCHHEEEEE
DGSAAKLGIDKLGYEPDQQKLFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRN
CCCCHHCCHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEHHHCCCCCCC
ISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIRDLETAEIAIKA
CCCCCCCEEEECCCCHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEE
AQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
CCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EHALLAEGFTPEQVANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQ
CHHHHHCCCCHHHHHCHHHHHHCCCHHHHCCCCCCCCEEEEECCCCCCCEEEECCCCCHH
IAEAAQKIGIRDLRQSALMKASQGVTSLAEINRVTKD
HHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNSVSTTNLVGITGIARRLVQDGALEETAARTAMEQAAAAKVPLPQWFAENRLVSASQLA
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHH
AANAVEFGMPLMDVSVFDANQNAVKLVSEELLQKYHVLPLFKRGNRLFVGVSNPTQTRAL
HHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCCCHHHHH
DDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASFGSSLGNDDEEMGDLDVSTGDEDMGA
HHHHHHCCEEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
GGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHH
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEHHHHHHHHCCHHEEEEE
DGSAAKLGIDKLGYEPDQQKLFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRN
CCCCHHCCHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEHHHCCCCCCC
ISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIRDLETAEIAIKA
CCCCCCCEEEECCCCHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEE
AQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
CCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EHALLAEGFTPEQVANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQ
CHHHHHCCCCHHHHHCHHHHHHCCCHHHHCCCCCCCCEEEEECCCCCCCEEEECCCCCHH
IAEAAQKIGIRDLRQSALMKASQGVTSLAEINRVTKD
HHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]