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The map label for this gene is asnB [H]

Identifier: 84623069

GI number: 84623069

Start: 1535529

End: 1537469

Strand: Direct

Name: asnB [H]

Synonym: XOO_1412

Alternate gene names: 84623069

Gene position: 1535529-1537469 (Clockwise)

Preceding gene: 84623068

Following gene: 84623070

Centisome position: 31.08

GC content: 67.49

Gene sequence:

>1941_bases
ATGTGCGGATTGGCAGGACTGTTGCTGGCTTCGCCGCGTCTGCATGGCGAACAGCTCGATGCGCTGGTGCGGCCGATGGG
CGCCGCTTTGCGCCATCGCGGCCCGGACGATGCCGGCACTTGGTGCGATGCGCAGGCCGGCGTTGCCTTGGCGCATCAAC
GGCTAAGCATTCTGGATCTGTCGCCGCTCGGCCACCAGCCGATGCGTTCGGCTGATGGCCGCTATGTGCTGGCCTATAAC
GGCGAGGTCTATAACTTCGCGCAGCTGCGCGCGGCACTGTCCGCGCTCGGGCATCGCTTCCGCGGCCACTCCGATACCGA
AGTGTTGCTGGCGGCCGTGGTGGAGTGGGGCCTGGACGAGACGTTGACCCGCTGCAACGGCATGTTCGCCATTGCGTTGT
GGGACGAGCGCGACAACTGCCTGTTCCTGGCGCGCGACCGGGTCGGCAAAAAGCCGCTCTACTACGGGTGGGCCGGCGAC
ACCTTGGTGTTCGGTTCCGAACTCAAGGCCTTGTGGCAGCACCCGGATTTCGACAACGGCGTCGATCGCAATGCGCTGAC
CTTGCTGTTGCGGCTGGGCTACATCCCGGCGCCGGCCTGCATCCACGCGCACACCTTCAAGCTGATGCCTGGCCGCGTGC
TGCGCATGGATGCGCAGGCCGTGGCGGCCGGCGCTGCCGCGCATCGTGCCGATCAGGCGCAACAACCGTTCTGGAACGCA
CGCGAGGCGATGCAACGCGCGCTCGCCGCACCATTCACCGGCAGCGACGCGCAGGCCGAAGAGCAGTTGGATAGCGTGCT
GCGCGATGCGGTGGCCCTGCGCATGGTGGCCGATGTGCCGGTGGGCGTGTTTCTTTCCGGTGGCACCGATTCGTCCATCG
TCACTGCGATGATGCAGGCGCAGAGCGCGCAGCCGGTGCACACCTTCAGCATCGGCTTCGAAGGCTCGCATCACGACGAA
GCGCCGCTGGCGCGCGAAGTCGCCACGCATCTGCACACCGACCACACCGAACTCTATGTCAGCGGCGCCGATGCGCTGGC
GGTGGTGCCGTGCCTGCCGGACATGTTCGACGAACCCTTCGCCGATGCCTCGCAAGTGCCGACGGCGTTGGTCGCACGGT
TGGCGCGCGGTGGGGTTACGGTGGCGCTGTCCGGCGACGGTGGCGACGAATTGTTCTTCGGCTATGGCCGCTATCAACGT
GCGCTGCGCAACTGGCGCATGCATGGCCTGGTACCTGGCCCGCTGCGTCGCCTGATGGCAGTGGCAGCGCGTCGCAATGG
CGAATCTTCGCGTACCGGCGGGCTCGCCGCGCTGGTGGCCGAGGCGGGCGCACGCGGCATCGGCGACATCTACCGCAACC
GGATTTCGCGCTGGCGCGACCCGGCAGCGGTGGTGCCGGGGGCGACCGAACCCGATAGTTTTTACAGCCTGGCCGACCCG
CTGCATGGGTCCGGCACCCCGGCCGACGCGATGATGCTGGCCGACTTCGCCGCGTATCTGCCCGACGATCTGCTGTGCAA
GGTGGACCGCACCACGATGGCGGTGGGGCTGGAGGCGCGCGCGCCGCTGCTGGATTGGCGCGTGGCCGAATTCGCCTGGT
CGCTGCCGCTGTCGCTGAAATATCGCGATGGAATGAGCAAGTATCTGCTTAAGCGCGTGTTGTGCCGCTACCTGCCGGAC
ACGATGGTGTACCGCGGCAAGCGTGGCTTCGGCGCGCCGGTGAGTGCATGGCTACGCGGCGATCTGCACGGCTGGGCGGA
CGATCTGCTGGCGCACGGCGCGTTGCAGCGCGAGGGCGTGTTCGCCGCCGATACCGTGGCTGGCCTGTGGCGCGACTTCA
ACGGCGGCGAGCGCAAATGGCATACGCACCTGTGGACGGTGCTGATGTTCCAGGCCTGGCAGGCGCACTGGCGCGAGCAA
CGCGCAGCCATCACGCGCTGA

Upstream 100 bases:

>100_bases
CATCGGCCTTCGCCATGCGCTTGCACGCGCGCGCGCTGCGCGTGACGCGTGTGGGGCACGCCCGGTTACTCCCCGTTGCC
AGCAGGCAGGAGCACTGACG

Downstream 100 bases:

>100_bases
TCGTGGTTGCGGCGACGCGCAATGGTGAGGTGCTGAAGATGGCGCTGCAGTGCGCGGCGTGTTGGCGTTGCTTGTCGCTT
CGGCCCTGCCGTCGGAGCGG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MCGLAGLLLASPRLHGEQLDALVRPMGAALRHRGPDDAGTWCDAQAGVALAHQRLSILDLSPLGHQPMRSADGRYVLAYN
GEVYNFAQLRAALSALGHRFRGHSDTEVLLAAVVEWGLDETLTRCNGMFAIALWDERDNCLFLARDRVGKKPLYYGWAGD
TLVFGSELKALWQHPDFDNGVDRNALTLLLRLGYIPAPACIHAHTFKLMPGRVLRMDAQAVAAGAAAHRADQAQQPFWNA
REAMQRALAAPFTGSDAQAEEQLDSVLRDAVALRMVADVPVGVFLSGGTDSSIVTAMMQAQSAQPVHTFSIGFEGSHHDE
APLAREVATHLHTDHTELYVSGADALAVVPCLPDMFDEPFADASQVPTALVARLARGGVTVALSGDGGDELFFGYGRYQR
ALRNWRMHGLVPGPLRRLMAVAARRNGESSRTGGLAALVAEAGARGIGDIYRNRISRWRDPAAVVPGATEPDSFYSLADP
LHGSGTPADAMMLADFAAYLPDDLLCKVDRTTMAVGLEARAPLLDWRVAEFAWSLPLSLKYRDGMSKYLLKRVLCRYLPD
TMVYRGKRGFGAPVSAWLRGDLHGWADDLLAHGALQREGVFAADTVAGLWRDFNGGERKWHTHLWTVLMFQAWQAHWREQ
RAAITR

Sequences:

>Translated_646_residues
MCGLAGLLLASPRLHGEQLDALVRPMGAALRHRGPDDAGTWCDAQAGVALAHQRLSILDLSPLGHQPMRSADGRYVLAYN
GEVYNFAQLRAALSALGHRFRGHSDTEVLLAAVVEWGLDETLTRCNGMFAIALWDERDNCLFLARDRVGKKPLYYGWAGD
TLVFGSELKALWQHPDFDNGVDRNALTLLLRLGYIPAPACIHAHTFKLMPGRVLRMDAQAVAAGAAAHRADQAQQPFWNA
REAMQRALAAPFTGSDAQAEEQLDSVLRDAVALRMVADVPVGVFLSGGTDSSIVTAMMQAQSAQPVHTFSIGFEGSHHDE
APLAREVATHLHTDHTELYVSGADALAVVPCLPDMFDEPFADASQVPTALVARLARGGVTVALSGDGGDELFFGYGRYQR
ALRNWRMHGLVPGPLRRLMAVAARRNGESSRTGGLAALVAEAGARGIGDIYRNRISRWRDPAAVVPGATEPDSFYSLADP
LHGSGTPADAMMLADFAAYLPDDLLCKVDRTTMAVGLEARAPLLDWRVAEFAWSLPLSLKYRDGMSKYLLKRVLCRYLPD
TMVYRGKRGFGAPVSAWLRGDLHGWADDLLAHGALQREGVFAADTVAGLWRDFNGGERKWHTHLWTVLMFQAWQAHWREQ
RAAITR
>Mature_646_residues
MCGLAGLLLASPRLHGEQLDALVRPMGAALRHRGPDDAGTWCDAQAGVALAHQRLSILDLSPLGHQPMRSADGRYVLAYN
GEVYNFAQLRAALSALGHRFRGHSDTEVLLAAVVEWGLDETLTRCNGMFAIALWDERDNCLFLARDRVGKKPLYYGWAGD
TLVFGSELKALWQHPDFDNGVDRNALTLLLRLGYIPAPACIHAHTFKLMPGRVLRMDAQAVAAGAAAHRADQAQQPFWNA
REAMQRALAAPFTGSDAQAEEQLDSVLRDAVALRMVADVPVGVFLSGGTDSSIVTAMMQAQSAQPVHTFSIGFEGSHHDE
APLAREVATHLHTDHTELYVSGADALAVVPCLPDMFDEPFADASQVPTALVARLARGGVTVALSGDGGDELFFGYGRYQR
ALRNWRMHGLVPGPLRRLMAVAARRNGESSRTGGLAALVAEAGARGIGDIYRNRISRWRDPAAVVPGATEPDSFYSLADP
LHGSGTPADAMMLADFAAYLPDDLLCKVDRTTMAVGLEARAPLLDWRVAEFAWSLPLSLKYRDGMSKYLLKRVLCRYLPD
TMVYRGKRGFGAPVSAWLRGDLHGWADDLLAHGALQREGVFAADTVAGLWRDFNGGERKWHTHLWTVLMFQAWQAHWREQ
RAAITR

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=439, Percent_Identity=30.751708428246, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25147557, Length=408, Percent_Identity=26.4705882352941, Blast_Score=122, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI71993933, Length=408, Percent_Identity=26.4705882352941, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17560178, Length=461, Percent_Identity=25.1626898047722, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25147560, Length=376, Percent_Identity=25.531914893617, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321563, Length=476, Percent_Identity=27.5210084033613, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6325403, Length=452, Percent_Identity=26.7699115044248, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI45553209, Length=436, Percent_Identity=28.6697247706422, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70608; Mature: 70608

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLAGLLLASPRLHGEQLDALVRPMGAALRHRGPDDAGTWCDAQAGVALAHQRLSILDL
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEEEC
SPLGHQPMRSADGRYVLAYNGEVYNFAQLRAALSALGHRFRGHSDTEVLLAAVVEWGLDE
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHH
TLTRCNGMFAIALWDERDNCLFLARDRVGKKPLYYGWAGDTLVFGSELKALWQHPDFDNG
HHHHHCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCEEEECHHHHHHHCCCCCCCC
VDRNALTLLLRLGYIPAPACIHAHTFKLMPGRVLRMDAQAVAAGAAAHRADQAQQPFWNA
CCHHHHHHHHHHCCCCCCHHHHHHHHEECCCCEEEECHHHHHHHHHHHHHHHHCCCHHHH
REAMQRALAAPFTGSDAQAEEQLDSVLRDAVALRMVADVPVGVFLSGGTDSSIVTAMMQA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
QSAQPVHTFSIGFEGSHHDEAPLAREVATHLHTDHTELYVSGADALAVVPCLPDMFDEPF
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEECCCHHHHCCCC
ADASQVPTALVARLARGGVTVALSGDGGDELFFGYGRYQRALRNWRMHGLVPGPLRRLMA
CCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECHHHHHHHHHCCHHCCCCCHHHHHHHH
VAARRNGESSRTGGLAALVAEAGARGIGDIYRNRISRWRDPAAVVPGATEPDSFYSLADP
HHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHCC
LHGSGTPADAMMLADFAAYLPDDLLCKVDRTTMAVGLEARAPLLDWRVAEFAWSLPLSLK
CCCCCCCHHHHHHHHHHHHCCCHHEEEECCHHEEECCCCCCCHHHHHHHHHHHCCCCEEE
YRDGMSKYLLKRVLCRYLPDTMVYRGKRGFGAPVSAWLRGDLHGWADDLLAHGALQREGV
ECCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCHHHCCC
FAADTVAGLWRDFNGGERKWHTHLWTVLMFQAWQAHWREQRAAITR
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGLAGLLLASPRLHGEQLDALVRPMGAALRHRGPDDAGTWCDAQAGVALAHQRLSILDL
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEEEC
SPLGHQPMRSADGRYVLAYNGEVYNFAQLRAALSALGHRFRGHSDTEVLLAAVVEWGLDE
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHH
TLTRCNGMFAIALWDERDNCLFLARDRVGKKPLYYGWAGDTLVFGSELKALWQHPDFDNG
HHHHHCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCEEEECHHHHHHHCCCCCCCC
VDRNALTLLLRLGYIPAPACIHAHTFKLMPGRVLRMDAQAVAAGAAAHRADQAQQPFWNA
CCHHHHHHHHHHCCCCCCHHHHHHHHEECCCCEEEECHHHHHHHHHHHHHHHHCCCHHHH
REAMQRALAAPFTGSDAQAEEQLDSVLRDAVALRMVADVPVGVFLSGGTDSSIVTAMMQA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
QSAQPVHTFSIGFEGSHHDEAPLAREVATHLHTDHTELYVSGADALAVVPCLPDMFDEPF
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEECCCHHHHCCCC
ADASQVPTALVARLARGGVTVALSGDGGDELFFGYGRYQRALRNWRMHGLVPGPLRRLMA
CCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECHHHHHHHHHCCHHCCCCCHHHHHHHH
VAARRNGESSRTGGLAALVAEAGARGIGDIYRNRISRWRDPAAVVPGATEPDSFYSLADP
HHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHCC
LHGSGTPADAMMLADFAAYLPDDLLCKVDRTTMAVGLEARAPLLDWRVAEFAWSLPLSLK
CCCCCCCHHHHHHHHHHHHCCCHHEEEECCHHEEECCCCCCCHHHHHHHHHHHCCCCEEE
YRDGMSKYLLKRVLCRYLPDTMVYRGKRGFGAPVSAWLRGDLHGWADDLLAHGALQREGV
ECCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCHHHCCC
FAADTVAGLWRDFNGGERKWHTHLWTVLMFQAWQAHWREQRAAITR
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]