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The map label for this gene is pilT [H]

Identifier: 84622959

GI number: 84622959

Start: 1424606

End: 1425643

Strand: Direct

Name: pilT [H]

Synonym: XOO_1302

Alternate gene names: 84622959

Gene position: 1424606-1425643 (Clockwise)

Preceding gene: 84622955

Following gene: 84622960

Centisome position: 28.84

GC content: 61.85

Gene sequence:

>1038_bases
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCGCTGGACCATAAGCAGGTGCATGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTACGAGGAATTCCTCGAAGTCGACTTCTCGTTCGAAATTTTGTCGCTGGCGCGT
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCCTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCTTGATCCTGGTGACCGGCCCGACTGGTT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAATCAGCGCGAGGTGCGCCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGTTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCAGTGATTTCGCAGGC
GCTGCTGAAGAAAGTCGGCGGCGGGCGTACCGCAGCATGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTCGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCGCGCGAGTACGCCAAGGACAAGCGGATATTCGAATAG

Upstream 100 bases:

>100_bases
CGGCCCAAAACGGGTCCAGGCCGCTTCCCGCCAGCAGCCAGAACGCTATACTGACGGCTGGGGAGTACCTTCCAATCGCA
GCCTAGGGGAAAAGCAGCGC

Downstream 100 bases:

>100_bases
CCGGGATTGGGGATTCGGAATGGGGGATTCGCAATGGCGATGTCCCGGTCCGGACCCCGCTCTTGCGCATCCCGAATCTC
CACTCCCGAATCCCTGCTCC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEILSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVRRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Sequences:

>Translated_345_residues
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEILSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVRRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE
>Mature_345_residues
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEILSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVRRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=324, Percent_Identity=48.7654320987654, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1789723, Length=285, Percent_Identity=36.8421052631579, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786296, Length=306, Percent_Identity=33.0065359477124, Blast_Score=128, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38679; Mature: 38679

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQR
CCHHHHHHHHHHCCCCCEEECCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHC
RDYEEFLEVDFSFEILSLARFRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLID
CCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHEEHHHCCCCHHHHHHHC
QPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVEDPIEFVHTSQKCLINQREVRR
CCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHCCHHHHHCHHHHHH
DTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
HHCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEEECCCCCEEEEEECCCHHHHHHHHH
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHH
MYSSIQTGQQYGMQTLDQHLQDLVKRSLITRNQAREYAKDKRIFE
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQR
CCHHHHHHHHHHCCCCCEEECCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHC
RDYEEFLEVDFSFEILSLARFRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLID
CCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHEEHHHCCCCHHHHHHHC
QPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVEDPIEFVHTSQKCLINQREVRR
CCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHCCHHHHHCHHHHHH
DTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
HHCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEEECCCCCEEEEEECCCHHHHHHHHH
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHH
MYSSIQTGQQYGMQTLDQHLQDLVKRSLITRNQAREYAKDKRIFE
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]