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The map label for this gene is pilT [H]

Identifier: 84622960

GI number: 84622960

Start: 1425757

End: 1426887

Strand: Direct

Name: pilT [H]

Synonym: XOO_1303

Alternate gene names: 84622960

Gene position: 1425757-1426887 (Clockwise)

Preceding gene: 84622959

Following gene: 84622961

Centisome position: 28.86

GC content: 60.65

Gene sequence:

>1131_bases
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGAATGGCAAGATCAGCCCGATTACCCAGACGCCGCTGACTGCGCAGCAGAGCCGCGATCTGG
TGTTGAACGTGATGACTCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTTTCAGGG
GTGGGGCGTTTTCGTGTGAGCTGTTTCTACCAGCGTAATCAGGTCGGTATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCCTGTCTCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCGTCATCTTCGTCGGCG
CCACCGGTGCCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGAAAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAATCAGGCGATG
GACCGCATCATCAACTTCTTCCCCAAAGACCGCCGCAATCAGCTGCTGATGGACCTGTCGCTCAATCTCAAGGGCGTGGT
GGCGCAGCAGCTGATTCCGACGCCGGATGGCCGCAGCCGTCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAAATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACTTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCTGGCGAAATCAGCTACGAAGACGCATTGCGCTACGCCGACTCGCAGAACGA
AGTGCGCTTGCGCATCAAGCTCTCGCAGGGGGGCGATGCCAAGACCTTGTCGCAGGGCATGGATGGTGTGGAGATTTCCG
AAGTTCGATAA

Upstream 100 bases:

>100_bases
TTCGGAATGGGGGATTCGCAATGGCGATGTCCCGGTCCGGACCCCGCTCTTGCGCATCCCGAATCTCCACTCCCGAATCC
CTGCTCCAGAGGAGCACGTC

Downstream 100 bases:

>100_bases
GCGCGTCGGCAGGCACGCAGGGGCGCGCCCATGCAAAGAAACCAATTCGGCCCGCTCGAGGGCGGGTCTGCCGGTGCCAT
CGAGCGGATGCTGGCTGCGT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLSPVIKTLAMTKRGIVIFVGATGAGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPKDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGMDGVEISEVR

Sequences:

>Translated_376_residues
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLSPVIKTLAMTKRGIVIFVGATGAGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPKDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGMDGVEISEVR
>Mature_375_residues
STIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSGV
GRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLSPVIKTLAMTKRGIVIFVGATGAGKSTSLAAMIGYRNQNSTGHIITI
EDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMD
RIINFFPKDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTFD
QSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGMDGVEISEVR

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=321, Percent_Identity=33.6448598130841, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1789723, Length=155, Percent_Identity=38.0645161290323, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786296, Length=261, Percent_Identity=26.8199233716475, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41969; Mature: 41838

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTP
CCCCCHHHHHHHHHHCCCCCEEEECCCCCEEEECCEECCCCCCCCCCCCCCCEEEEEECC
SQREEFEKTHECNFAIGVSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLSPVIKT
CHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHH
LAMTKRGIVIFVGATGAGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHEGCIITQRE
HHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEECCHHHHHHCCCCEEEEECC
VGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
CCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEECCCCEEEEEEECCCHHHHH
DRIINFFPKDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDG
HHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHCCC
EIHKLKEIMKESTNLGMRTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDA
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEEECCCCCH
KTLSQGMDGVEISEVR
HHHHCCCCCCEEECCC
>Mature Secondary Structure 
STIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTP
CCCCHHHHHHHHHHCCCCCEEEECCCCCEEEECCEECCCCCCCCCCCCCCCEEEEEECC
SQREEFEKTHECNFAIGVSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLSPVIKT
CHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHH
LAMTKRGIVIFVGATGAGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHEGCIITQRE
HHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEECCHHHHHHCCCCEEEEECC
VGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
CCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEECCCCEEEEEEECCCHHHHH
DRIINFFPKDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDG
HHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHCCC
EIHKLKEIMKESTNLGMRTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDA
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEEECCCCCH
KTLSQGMDGVEISEVR
HHHHCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]