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The map label for this gene is gapA [H]

Identifier: 84622754

GI number: 84622754

Start: 1195335

End: 1196336

Strand: Direct

Name: gapA [H]

Synonym: XOO_1097

Alternate gene names: 84622754

Gene position: 1195335-1196336 (Clockwise)

Preceding gene: 84622750

Following gene: 84622755

Centisome position: 24.2

GC content: 61.88

Gene sequence:

>1002_bases
ATGGCAATCAAGGTTGGCATCAACGGATTCGGTCGCATCGGCCGCAACGTGCTGCGCTCTGCGGTGCAGAACTTCGCCAA
TGACATCGAGATCGTGGCCATCAATGACCTGCTCGAACCCGACTATCTGGCCTACATGCTGCAGTACGACTCGGTGCATG
GCCGCTTCAAGGCCGACGTCTCGGTCGACGGCAACACGCTGATCGTCAATGGCAAGAAGATCCGCCTGACCCAGGAACGC
GACCCGGCCAACCTCAAGTGGGATGCGGTGGGCGCCGACGTGGTGATCGAGTCCACCGGCCTGTTCCTGACCAAGGACAC
CGCACAGAAGCACATCGACGCCGGTGCCAAGAAGGTGATCCTGTCCGCACCGTCCAAGGACGACACCCCGATGTTCGTCT
ACGGCGTCAACGACAAGACCTACAAGGGCGAAGCGATCGTCTCCAACGCCTCGTGCACCACCAACTGCCTGGCGCCGTTG
GCCAAGGTGATCAACGACAAGTGGGGCATCAAGCGCGGCCTGATGACCACCGTGCATGCGGCCACTGCAACGCAGAAGAC
TGTCGATGGCCCCTCGAACAAGGATTGGCGCGGCGGCCGCGGCATCCTGGAGAACATCATTCCGTCCTCCACCGGCGCGG
CCAAGGCCGTGGGCGTGGTGATTCCCGCGCTCAACAAGAAGCTCACCGGCATGAGCTTTCGCGTGCCGACCTCCGACGTC
TCGGTGGTCGACCTCACCGTTGAACTGGAAAAGCCGGCCACCTACGCTGAAATCTGCGCCGAAGTGAAGGCACAGAGCGA
AGGCGCGCTGAAGGGCGTGCTGGGGTATACCGAAGACAAGGTCGTGGCCACCGATTTCCGCGGCGAGACCTGCACCTCGG
TGTTCGACGCCGACGCTGGCATCGCGCTGGACTCCACCTTCGTCAAGCTGGTGTCCTGGTATGACAACGAGTGGGGCTAC
TCCAACAAGTGCCTGGAGATGGTGCGCGTTGTCGCCAAGTAA

Upstream 100 bases:

>100_bases
CTTCCAGCGCCCGCAACACACGGCTGCGGACAAGCGACGCTGCTAGAATGACAGCCCCTTTCGATCCGCCGCGCTGTCGC
GGCCGCAGGAGCTAGTGAAC

Downstream 100 bases:

>100_bases
ACGATGTGTGGTTAAACATAAAAAAGCGCCTTTCGGCGCTTTTTTATTTCCGCATTTACCAATATGTGTACAGGGCCGGC
GTCCGGCTCACTCTGCCGCA

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MAIKVGINGFGRIGRNVLRSAVQNFANDIEIVAINDLLEPDYLAYMLQYDSVHGRFKADVSVDGNTLIVNGKKIRLTQER
DPANLKWDAVGADVVIESTGLFLTKDTAQKHIDAGAKKVILSAPSKDDTPMFVYGVNDKTYKGEAIVSNASCTTNCLAPL
AKVINDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPALNKKLTGMSFRVPTSDV
SVVDLTVELEKPATYAEICAEVKAQSEGALKGVLGYTEDKVVATDFRGETCTSVFDADAGIALDSTFVKLVSWYDNEWGY
SNKCLEMVRVVAK

Sequences:

>Translated_333_residues
MAIKVGINGFGRIGRNVLRSAVQNFANDIEIVAINDLLEPDYLAYMLQYDSVHGRFKADVSVDGNTLIVNGKKIRLTQER
DPANLKWDAVGADVVIESTGLFLTKDTAQKHIDAGAKKVILSAPSKDDTPMFVYGVNDKTYKGEAIVSNASCTTNCLAPL
AKVINDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPALNKKLTGMSFRVPTSDV
SVVDLTVELEKPATYAEICAEVKAQSEGALKGVLGYTEDKVVATDFRGETCTSVFDADAGIALDSTFVKLVSWYDNEWGY
SNKCLEMVRVVAK
>Mature_332_residues
AIKVGINGFGRIGRNVLRSAVQNFANDIEIVAINDLLEPDYLAYMLQYDSVHGRFKADVSVDGNTLIVNGKKIRLTQERD
PANLKWDAVGADVVIESTGLFLTKDTAQKHIDAGAKKVILSAPSKDDTPMFVYGVNDKTYKGEAIVSNASCTTNCLAPLA
KVINDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPALNKKLTGMSFRVPTSDVS
VVDLTVELEKPATYAEICAEVKAQSEGALKGVLGYTEDKVVATDFRGETCTSVFDADAGIALDSTFVKLVSWYDNEWGYS
NKCLEMVRVVAK

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=332, Percent_Identity=60.8433734939759, Blast_Score=407, Evalue=1e-114,
Organism=Homo sapiens, GI7657116, Length=330, Percent_Identity=57.2727272727273, Blast_Score=389, Evalue=1e-108,
Organism=Escherichia coli, GI1788079, Length=333, Percent_Identity=73.2732732732733, Blast_Score=493, Evalue=1e-141,
Organism=Escherichia coli, GI1789295, Length=335, Percent_Identity=37.0149253731343, Blast_Score=245, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI17568413, Length=336, Percent_Identity=61.0119047619048, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32566163, Length=336, Percent_Identity=61.0119047619048, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17534677, Length=336, Percent_Identity=60.7142857142857, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17534679, Length=336, Percent_Identity=60.1190476190476, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322409, Length=333, Percent_Identity=64.8648648648649, Blast_Score=427, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6322468, Length=333, Percent_Identity=64.2642642642643, Blast_Score=423, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6321631, Length=333, Percent_Identity=64.2642642642643, Blast_Score=422, Evalue=1e-119,
Organism=Drosophila melanogaster, GI17933600, Length=328, Percent_Identity=62.1951219512195, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI18110149, Length=328, Percent_Identity=62.1951219512195, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI85725000, Length=328, Percent_Identity=62.1951219512195, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI22023983, Length=328, Percent_Identity=62.1951219512195, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI19922412, Length=326, Percent_Identity=61.3496932515337, Blast_Score=390, Evalue=1e-109,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 35871; Mature: 35740

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKVGINGFGRIGRNVLRSAVQNFANDIEIVAINDLLEPDYLAYMLQYDSVHGRFKADV
CEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHEEEEHCCCCCCEEEEEE
SVDGNTLIVNGKKIRLTQERDPANLKWDAVGADVVIESTGLFLTKDTAQKHIDAGAKKVI
EECCCEEEEECEEEEEECCCCCCCEEEECCCCEEEEECCCEEEECCHHHHHHCCCCCEEE
LSAPSKDDTPMFVYGVNDKTYKGEAIVSNASCTTNCLAPLAKVINDKWGIKRGLMTTVHA
EECCCCCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
ATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPALNKKLTGMSFRVPTSDV
HHHCHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHEEEEECCCCCCCCCEEECCCCCC
SVVDLTVELEKPATYAEICAEVKAQSEGALKGVLGYTEDKVVATDFRGETCTSVFDADAG
EEEEEEEEECCCCCHHHHHHHHHCCCCCCHHHHCCCCCCCEEEECCCCCHHHHHHCCCCC
IALDSTFVKLVSWYDNEWGYSNKCLEMVRVVAK
CEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AIKVGINGFGRIGRNVLRSAVQNFANDIEIVAINDLLEPDYLAYMLQYDSVHGRFKADV
EEEEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHEEEEHCCCCCCEEEEEE
SVDGNTLIVNGKKIRLTQERDPANLKWDAVGADVVIESTGLFLTKDTAQKHIDAGAKKVI
EECCCEEEEECEEEEEECCCCCCCEEEECCCCEEEEECCCEEEECCHHHHHHCCCCCEEE
LSAPSKDDTPMFVYGVNDKTYKGEAIVSNASCTTNCLAPLAKVINDKWGIKRGLMTTVHA
EECCCCCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
ATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPALNKKLTGMSFRVPTSDV
HHHCHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHEEEEECCCCCCCCCEEECCCCCC
SVVDLTVELEKPATYAEICAEVKAQSEGALKGVLGYTEDKVVATDFRGETCTSVFDADAG
EEEEEEEEECCCCCHHHHHHHHHCCCCCCHHHHCCCCCCCEEEECCCCCHHHHHHCCCCC
IALDSTFVKLVSWYDNEWGYSNKCLEMVRVVAK
CEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852; 8366033 [H]