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The map label for this gene is rep [H]

Identifier: 84621878

GI number: 84621878

Start: 241419

End: 243395

Strand: Direct

Name: rep [H]

Synonym: XOO_0221

Alternate gene names: 84621878

Gene position: 241419-243395 (Clockwise)

Preceding gene: 84621875

Following gene: 84621879

Centisome position: 4.89

GC content: 62.97

Gene sequence:

>1977_bases
ATGTTCGGACTCAATCCCCCGCAAAGCGCCGCGGTGCTGCACTGTGAAGGTCCCTTGTTGGTGCTGGCCGGCGCCGGCAG
CGGCAAGACGCGCGTGATCGTGGAAAAGATCGCGCATCTGATCGCCATCGGCCGCTATCCGGCCAAGCGCATCGCCGCGA
TCACCTTCACCAACAAATCCGCCAAGGAAATGGGCGAGCGTGTGGCCAAGCGCATCCGCGGCGACGGTGCCGACGGCCTG
ACCATCTGCACCTTCCACGCGCTGGGCCTGAAGTTCCTGCAGATCGAGCACGCCGCCGCCGGTTTGAAGCGCGGCTTTTC
GATCTTCGATTCCGACGATGCCGCCGCGCAGATCAAGGACCTGATGCATGGTGCCAAGCCCGATGCGATCGAAGATGCCA
AGAACCTGATCTCGCGCGCCAAGAACGCCGGCCTGTCGCCCGAGCAGGCGATGGTGGCGGCACGCAGCAACCGCGAAAAA
GAAGCGGCCAGCTTGTACGAGCGCTACCAGGCGCGGTTGACCACCTTCAACGCAGTGGATTTCGATGACCTGATCCGCCT
GCCGGTGCAGATTCTGGAAGCAAACGAAGACATCGTGATGGGCTGGCGCGAGCGCATCGGCTATTTGCTGGTGGACGAGT
GCCAGGACACCAACGATGCGCAGTACCGCCTGCTGAAGATGCTGGCCGGGCCGCGCGGCAACTTCACCTGCGTGGGCGAT
GACGACCAGAGTATCTACGCGTGGCGCGGCGCCAATCCGGAAAACCTGCAGCAGATGGCGCGCGATTATCCGGCGCTGAA
AATCATCAAGCTGGAGCAGAACTACCGCTGCTCCAATCGTGTGCTGCGTGCGGCCAATGCGTTGATCGCGCACAACCCAC
ACGAGCATTTGAAGACACTGTGGAGCGACCAGGCCGACGGCGAGCGCATCCGCGTGTGGGAATGCCGCGACAGCGAGCAC
GAGGCCGAAAAGGTTGCTGCGGAGATTTCGTTCCTTGGCACCGCCAAGCAGGTGCCGTGGAGCGATTTCTGCATTCTGTT
CCGTGGAAATTTTCAGTCGCGGCCGCTGGAAAAAGCGCTGCAGCTGTTGCGCGTGCCGTATCACCTGACTGGTGGTACCG
CGTTTCTGGAGCGGCAGGAGGTCAAGGATCTGCTGTCGTGGTTGCGGTTGATCGTCAACCCGGACGACGATGTGGCGTTC
TTGCGTGCGGTGCAATCGCCCAAGCGCGAAGTCGGTGCGACCTCGCTGGCGCGTCTGGCCGAGTTGGCCAGCGCCAAATC
GGTGCCGATGTCGCGCGCGGCCGAGTCGATGGGCGCCTTGCAGCACCTGCCGCCCCGCGCCGCCAATGGGTTGAGTGCCT
TTACCGACATCGTGCGCGACATGCGCGAGCACTCGGCCAGCATGCCGGCCGGCGAACTGGTGCGCACGCTCGCGGAAAAA
TCCGGACTGCTCAACGACCTGCGCAATCAGTCCAAGGACGAAACCGGCTTCCAGCGGCGCAAGCGCAATCTGGACGAGTT
GGCCGAGTGGTTCGAAGGCGGCCCGCGTGGTGCAAGCGCCAGCGATCTGGCTGCGCAGCTGGCGCTGCTGTCGCGCAACG
ACAAGGACGATGGCGGCAACCAGGTGCGCATGATGACCATGCATGCGTCCAAGGGTCTGGAGTTCCGCTATGTGTTCATC
GTCGGCTGCGAGGACGGCGTGCTGCCGCACGAAGTGAGCCTGGAAGAAGGCAATCTGCAGGAAGAACGCCGTTTGTTGTA
CGTGGGCATCACCCGCGCCAAGGAGCAGTTGTGGATGAGCCACAGCAAGCTGACGCGCAAGTTCGGCGAGCATGTGCGAC
TGAAGCCGAGCCGGTTTTTCGATGAAATTCCTGCCGAAGAACTGCAGCGCGATGGCGCCGACCCGGTGGCCGACGCTGAG
CGCAAGAAAGAACGCGCCAGTGCGGGATTGGCTGCGATCCAGGCATTGTTCGACTGA

Upstream 100 bases:

>100_bases
CGCTGTGAAGCGAAGCCTAGCGGCAATGGAGACATGAACCCATCGTGCAGGCCAGCCGCAGGCTGCCCCGGTACAATGGC
CTGCTCTGTCGGAAGCCCCT

Downstream 100 bases:

>100_bases
CGCGCAGTGCGCCGTTGCACGCGCACCTGCCGCCCATCGCTTACCAGTGACGCGCCGGCACGGCAGTGTGGTTTTTCATG
ATGTCGGAGTGTGCCGTGAC

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MFGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKSAKEMGERVAKRIRGDGADGL
TICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKDLMHGAKPDAIEDAKNLISRAKNAGLSPEQAMVAARSNREK
EAASLYERYQARLTTFNAVDFDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
DDQSIYAWRGANPENLQQMARDYPALKIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTLWSDQADGERIRVWECRDSEH
EAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKALQLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDVAF
LRAVQSPKREVGATSLARLAELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDIVRDMREHSASMPAGELVRTLAEK
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGNQVRMMTMHASKGLEFRYVFI
VGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMSHSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAE
RKKERASAGLAAIQALFD

Sequences:

>Translated_658_residues
MFGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKSAKEMGERVAKRIRGDGADGL
TICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKDLMHGAKPDAIEDAKNLISRAKNAGLSPEQAMVAARSNREK
EAASLYERYQARLTTFNAVDFDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
DDQSIYAWRGANPENLQQMARDYPALKIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTLWSDQADGERIRVWECRDSEH
EAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKALQLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDVAF
LRAVQSPKREVGATSLARLAELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDIVRDMREHSASMPAGELVRTLAEK
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGNQVRMMTMHASKGLEFRYVFI
VGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMSHSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAE
RKKERASAGLAAIQALFD
>Mature_658_residues
MFGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKSAKEMGERVAKRIRGDGADGL
TICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKDLMHGAKPDAIEDAKNLISRAKNAGLSPEQAMVAARSNREK
EAASLYERYQARLTTFNAVDFDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
DDQSIYAWRGANPENLQQMARDYPALKIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTLWSDQADGERIRVWECRDSEH
EAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKALQLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDVAF
LRAVQSPKREVGATSLARLAELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDIVRDMREHSASMPAGELVRTLAEK
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGNQVRMMTMHASKGLEFRYVFI
VGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMSHSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAE
RKKERASAGLAAIQALFD

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=670, Percent_Identity=44.4776119402985, Blast_Score=533, Evalue=1e-152,
Organism=Escherichia coli, GI2367296, Length=643, Percent_Identity=38.2581648522551, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1787196, Length=335, Percent_Identity=27.7611940298507, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322369, Length=732, Percent_Identity=26.3661202185792, Blast_Score=194, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 73154; Mature: 73154

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKS
CCCCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEECCHH
AKEMGERVAKRIRGDGADGLTICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKD
HHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LMHGAKPDAIEDAKNLISRAKNAGLSPEQAMVAARSNREKEAASLYERYQARLTTFNAVD
HHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
FDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
HHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECC
DDQSIYAWRGANPENLQQMARDYPALKIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTL
CCCCEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHH
WSDQADGERIRVWECRDSEHEAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKAL
HCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHH
QLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDVAFLRAVQSPKREVGATSLARLA
HHHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHH
ELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDIVRDMREHSASMPAGELVRTLAEK
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGN
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
QVRMMTMHASKGLEFRYVFIVGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMS
EEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHCEEEEEEHHHHHHHHHHH
HSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAERKKERASAGLAAIQALFD
HHHHHHHHHHCEECCHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKS
CCCCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEECCHH
AKEMGERVAKRIRGDGADGLTICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKD
HHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LMHGAKPDAIEDAKNLISRAKNAGLSPEQAMVAARSNREKEAASLYERYQARLTTFNAVD
HHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
FDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
HHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECC
DDQSIYAWRGANPENLQQMARDYPALKIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTL
CCCCEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHH
WSDQADGERIRVWECRDSEHEAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKAL
HCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHH
QLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDVAFLRAVQSPKREVGATSLARLA
HHHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHH
ELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDIVRDMREHSASMPAGELVRTLAEK
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGN
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
QVRMMTMHASKGLEFRYVFIVGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMS
EEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHCEEEEEEHHHHHHHHHHH
HSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAERKKERASAGLAAIQALFD
HHHHHHHHHHCEECCHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]