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The map label for this gene is recF [H]

Identifier: 84621660

GI number: 84621660

Start: 3999

End: 5087

Strand: Direct

Name: recF [H]

Synonym: XOO_0003

Alternate gene names: 84621660

Gene position: 3999-5087 (Clockwise)

Preceding gene: 84621659

Following gene: 84621661

Centisome position: 0.08

GC content: 64.0

Gene sequence:

>1089_bases
ATGCATGTAATGCGGTTGTCCATTCATCGGCTTCGTCGGTTCCAGACCGTCGAGCTACATCCGGCCAGTGCATTGAATCT
GCTCACCGGCGACAACGGTGCAGGCAAGACCAGCGTGCTCGAGGCGCTGCACCTGATGGCATATGGCCGCAGCTTCCGTG
GCCGCGTCCGTGACGGCCTGATCCAACAAGGCGCGAACGACCTTGAAGTCTTCGTGGAATGGAAAGAAGGCGGCAGTGCT
GCAGGCGAGCGGACACGTCGCGCCGGCCTACGTCATAGCGGCCAGGAATGGACGGGGCGTCTGGATGGCGAAGACGTTGC
ACAGTTGGGGTCTTTGTGTGCTGCGTTGGCCGTGGTGACCTTTGAGCCCGGCAGCCACGTGTTGATCAGCGGCGGTGGTG
AACCCCGCCGTCGCTTCCTGGACTGGGGGCTGTTCCACGTGGAACCCGACTTCCTCGCCTTGTGGCGTCGCTACGTGCGC
GCGCTCAAACAGCGCAATGCGTTGCTCAAACAAGGCGCGCAGCCGCGCATGCTGGATGCCTGGGATCATGAACTCGCCGA
ATCCGGGGAAACGCTGACATCCCGGCGCATGCGCTACCTGGAACGCCTGCAGGACCGGCTGATTCCGGTGGCAGACGTGA
TCGCACCGTCGCTTGGGCTGTCCGCGTTGACGTTCGCCCCAGGCTGGAAACGCCACGAGGTCTCGCTTGCCGATGCCCTT
TTGTTAGCCCGCGATCGCGACCGCCAGAACGGTTACACCTCGCAAGGCCCACACCGCGCGGATTGGATGCCTCACTTCGA
CGTGCTCCCTGGCAAAGATGCACTTTCTCGCGGGCAAGCCAAGCTCACTGCCCTCGCCTGCCTCCTTGCACAAGCAGAAG
ACTTTGCGTTCGAGCGAAGCGAATGGCCGGTGATCGCGCTGGACGACCTGGGTTCGGAGCTGGACCGGCACCACCAGGCG
CGCGTCCTGCACCGGCTGGTCTCGGCGCCAGCGCAGATGCTGATCACCGGGACCGAGATCCCGCCCGGGCTCGCGGACGC
CGGTGCGCTATTGCACAGGTTCCACGTGGAACACGGTCAGGTCGACTGA

Upstream 100 bases:

>100_bases
TCCGAGACCGTCCGTTTGCATGTCTGCGCGGGTTTCATGCCGATCACGTATCCTTTGAGCCGACCGGCATGCCGGATTAC
ACGCCAGAGCACACCGATCA

Downstream 100 bases:

>100_bases
AAGCCCCTCGGACTGAGCTCAACGGCCCGGGTAGGCCGACTGGTATAATTTGCTTCGCTATCCCCCCTTTGTCCACGGTG
CGAAGCGGCTGCGGCCGTCT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MHVMRLSIHRLRRFQTVELHPASALNLLTGDNGAGKTSVLEALHLMAYGRSFRGRVRDGLIQQGANDLEVFVEWKEGGSA
AGERTRRAGLRHSGQEWTGRLDGEDVAQLGSLCAALAVVTFEPGSHVLISGGGEPRRRFLDWGLFHVEPDFLALWRRYVR
ALKQRNALLKQGAQPRMLDAWDHELAESGETLTSRRMRYLERLQDRLIPVADVIAPSLGLSALTFAPGWKRHEVSLADAL
LLARDRDRQNGYTSQGPHRADWMPHFDVLPGKDALSRGQAKLTALACLLAQAEDFAFERSEWPVIALDDLGSELDRHHQA
RVLHRLVSAPAQMLITGTEIPPGLADAGALLHRFHVEHGQVD

Sequences:

>Translated_362_residues
MHVMRLSIHRLRRFQTVELHPASALNLLTGDNGAGKTSVLEALHLMAYGRSFRGRVRDGLIQQGANDLEVFVEWKEGGSA
AGERTRRAGLRHSGQEWTGRLDGEDVAQLGSLCAALAVVTFEPGSHVLISGGGEPRRRFLDWGLFHVEPDFLALWRRYVR
ALKQRNALLKQGAQPRMLDAWDHELAESGETLTSRRMRYLERLQDRLIPVADVIAPSLGLSALTFAPGWKRHEVSLADAL
LLARDRDRQNGYTSQGPHRADWMPHFDVLPGKDALSRGQAKLTALACLLAQAEDFAFERSEWPVIALDDLGSELDRHHQA
RVLHRLVSAPAQMLITGTEIPPGLADAGALLHRFHVEHGQVD
>Mature_362_residues
MHVMRLSIHRLRRFQTVELHPASALNLLTGDNGAGKTSVLEALHLMAYGRSFRGRVRDGLIQQGANDLEVFVEWKEGGSA
AGERTRRAGLRHSGQEWTGRLDGEDVAQLGSLCAALAVVTFEPGSHVLISGGGEPRRRFLDWGLFHVEPDFLALWRRYVR
ALKQRNALLKQGAQPRMLDAWDHELAESGETLTSRRMRYLERLQDRLIPVADVIAPSLGLSALTFAPGWKRHEVSLADAL
LLARDRDRQNGYTSQGPHRADWMPHFDVLPGKDALSRGQAKLTALACLLAQAEDFAFERSEWPVIALDDLGSELDRHHQA
RVLHRLVSAPAQMLITGTEIPPGLADAGALLHRFHVEHGQVD

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=362, Percent_Identity=33.4254143646409, Blast_Score=178, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40261; Mature: 40261

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVMRLSIHRLRRFQTVELHPASALNLLTGDNGAGKTSVLEALHLMAYGRSFRGRVRDGL
CCHHHHHHHHHHHHCEEEECCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IQQGANDLEVFVEWKEGGSAAGERTRRAGLRHSGQEWTGRLDGEDVAQLGSLCAALAVVT
HHCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHEE
FEPGSHVLISGGGEPRRRFLDWGLFHVEPDFLALWRRYVRALKQRNALLKQGAQPRMLDA
ECCCCEEEEECCCCHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
WDHELAESGETLTSRRMRYLERLQDRLIPVADVIAPSLGLSALTFAPGWKRHEVSLADAL
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHH
LLARDRDRQNGYTSQGPHRADWMPHFDVLPGKDALSRGQAKLTALACLLAQAEDFAFERS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EWPVIALDDLGSELDRHHQARVLHRLVSAPAQMLITGTEIPPGLADAGALLHRFHVEHGQ
CCCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHHHHHHHHHHCCCC
VD
CC
>Mature Secondary Structure
MHVMRLSIHRLRRFQTVELHPASALNLLTGDNGAGKTSVLEALHLMAYGRSFRGRVRDGL
CCHHHHHHHHHHHHCEEEECCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IQQGANDLEVFVEWKEGGSAAGERTRRAGLRHSGQEWTGRLDGEDVAQLGSLCAALAVVT
HHCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHEE
FEPGSHVLISGGGEPRRRFLDWGLFHVEPDFLALWRRYVRALKQRNALLKQGAQPRMLDA
ECCCCEEEEECCCCHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
WDHELAESGETLTSRRMRYLERLQDRLIPVADVIAPSLGLSALTFAPGWKRHEVSLADAL
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHH
LLARDRDRQNGYTSQGPHRADWMPHFDVLPGKDALSRGQAKLTALACLLAQAEDFAFERS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EWPVIALDDLGSELDRHHQARVLHRLVSAPAQMLITGTEIPPGLADAGALLHRFHVEHGQ
CCCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHHHHHHHHHHCCCC
VD
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]