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The map label for this gene is gyrB [H]

Identifier: 84621661

GI number: 84621661

Start: 5219

End: 7663

Strand: Direct

Name: gyrB [H]

Synonym: XOO_0004

Alternate gene names: 84621661

Gene position: 5219-7663 (Clockwise)

Preceding gene: 84621660

Following gene: 84621662

Centisome position: 0.11

GC content: 64.34

Gene sequence:

>2445_bases
ATGACCGACGAACAAAACACCCCGCCAACACCCAACGGCACTTACGACTCCAGCAAGATCACCGTGCTGCGTGGCCTGGA
AGCCGTCCGCAAGCGCCCCGGCATGTACATCGGCGATGTCCATGACGGCACCGGCCTGCATCACATGGTGTTCGAGGTGG
TCGACAACTCGGTCGACGAAGCCCTGGCCGGGCATGCCGACGACATCGTGGCGAAAATCCTGGTCGATGGCTCGGTGGCG
GTGTCAGACAACGGACGCGGCGTGCCGGTCGACATTCACAAGGAAGAAGGCGTGTCCGCGGCCGAGGTGATCCTCACCGT
GCTGCATGCCGGCGGCAAGTTCGACGACAACAGCTACAAGGTCTCCGGCGGCCTGCATGGCGTGGGCGTTTCGGTGGTCA
ACGCGCTGTCCGAGCACCTGTGGCTGGACATCTGGCGCGATGGTTTCCACTACCAGCAGGAATATGCGCTGGGCGAGCCG
CAGTACCCGCTCAAGCAGCTGGAAGCATCGACCAAGCGCGGCACCACGCTGCGCTTCAAGCCGTCCGCGGCCATCTTCAG
TGACGTCGAGTTCCACTACGACATCCTGGCGCGGCGCCTGCGTGAGCTGTCGTTCCTCAATTCCGGCGTCAAGATCACCC
TGATCGACGAGCGTGGCGAAGGCCGTCGCGACGATTTCCATTACGAAGGCGGCATCCGCAGCTTCGTGGAGCATCTTGCC
CAGCTGAAGTCGCCGCTGCACCCGAACGTGATCTCGGTGACCGGCGAGCACAATGGCATTGTGGTGGACGTGGCCCTGCA
GTGGACCGACGCCTACCAGGAAACCATGTACTGCTTCACCAACAACATCCCGCAGAAGGATGGCGGCACCCACTTGGCCG
GCTTCCGCGCCGCGCTGACGCGCGTGCTGGGCACCTACATCGAGCAGAACGGCATCGCCAAGCAGGCCAAGGTTGCGCTG
ACCGGTGACGACATGCGCGAAGGCATGATCGCAGTGCTGTCGGTCAAAGTGCCCGACCCCAGCTTCTCCTCGCAGACCAA
GGAAAAGCTGGTCAGCTCGGACGTGCGCCCGGCGGTGGAAAACGCCTTCGGTGCGCGCTTGCAGGAGTTCCTGCAAGAGA
ACCCGAACGAAGCCAAGGCCATCACTGGCAAGATCGTCGATGCCGCGCGTGCGCGCGAAGCCGCGCGCAAGGCGCGCGAC
CTCACCCGCCGCAAGGGCGCGCTGGATATCGCCGGCCTGCCGGGCAAGCTGGCCGACTGCCAGGAAAAAGATCCGGCGCT
GTCCGAACTCTTCATTGTCGAGGGTGACTCGGCAGGTGGCTCGGCCAAGCAGGGACGCAACCGCAAGAACCAGGCGGTGC
TGCCGTTGCGCGGCAAGATCCTCAACGTGGAACGTGCGCGCTTCGACCGCATGCTGGCGTCGGACCAGGTCGGCACGCTG
ATCACCGCACTGGGCACCGGCATCGGCCGCGACGAGTACAACCCGGACAAACTGCGCTACCACCGCATCATCCTGATGAC
CGACGCCGACGTCGACGGCTCGCACATCCGTACCCTGTTGCTGACCTTCTTCTACCGGCAGATGCCGGAGCTGATCGAGC
GGGGCTACATCTACATCGGCCTGCCGCCGCTGTACAAGCTCAAGCAGGGCAAGAGCGAGCTGTATCTGAAGGACGATGCA
GCGCTCAACGCCTACCTGGCCAGCAATGCGGTCGAAGGTGCGGCGCTGATTCCGGCCACCGACGAGCCGCCGATCACCGG
CGAAGCGCTGGAAAAACTGCTGCTGCTGTTCACCAGCGCCAACGAAGCGATTGCGCGCACCGCGCACCGCTACGACCCGG
CTTTGCTGACCGCATTGATCGACCTGCCGCCGCTGGATGTGGAAACACTGCAGGCCGAAGGCAATCAGCATCCGGCCCTG
GATGCGCTGCAGGCAGTGCTCAATCGCGGTACCCTGGGCACAGCGCGCTACCAGTTGCGCTTCGACCCGGCCACCGAGAA
CGCGCCTGCCACGCTGGTGGCGATCCGCCGTCATATGGGCGAAGAATTCACCCAGGTGCTGCCGATGGGGGCGTTCGAAA
GCGGCGAGCTGCGTCCGCTGCGTGAGGTATCGCTGGCCCTGCATGATCTGGTGCGCGAGGGCGCGCAGATCGTGCGCGGC
AACAAGAGCCACTCGATCACCAGCTTCGCGCAGGCGCACGCCTGGCTGCTGGACGAGGCCAAGAAGGGTCGCCAGGTCCA
GCGCTTCAAGGGCCTGGGCGAAATGAACGCCGAGCAGCTCTGGGAAACCACCGTCAACCCCGACACCCGTCGCCTGTTGC
AGGTGCGGATCGAGGATGCGGTGGCCGCCGACCAGATCTTCAGCACCCTGATGGGCGATGTGGTCGAACCACGCCGCGAC
TTCATCGAGGACAACGCGCTGAAGGTGTCCAACCTGGATATTTGA

Upstream 100 bases:

>100_bases
TAGGCCGACTGGTATAATTTGCTTCGCTATCCCCCCTTTGTCCACGGTGCGAAGCGGCTGCGGCCGTCTTTGCCTGTGTT
GTGGAGCCAACGGCACGCGC

Downstream 100 bases:

>100_bases
GCCACCAGTCCCGACCAGCACATCGCTATCGCGGCGTGCTGGTCGGTGCGGTGTTCGCAGGACGACGATGAGCACAGATC
TTCCACCGCCCCCGGCACCG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 814; Mature: 813

Protein sequence:

>814_residues
MTDEQNTPPTPNGTYDSSKITVLRGLEAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDEALAGHADDIVAKILVDGSVA
VSDNGRGVPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEHLWLDIWRDGFHYQQEYALGEP
QYPLKQLEASTKRGTTLRFKPSAAIFSDVEFHYDILARRLRELSFLNSGVKITLIDERGEGRRDDFHYEGGIRSFVEHLA
QLKSPLHPNVISVTGEHNGIVVDVALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAALTRVLGTYIEQNGIAKQAKVAL
TGDDMREGMIAVLSVKVPDPSFSSQTKEKLVSSDVRPAVENAFGARLQEFLQENPNEAKAITGKIVDAARAREAARKARD
LTRRKGALDIAGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLASDQVGTL
ITALGTGIGRDEYNPDKLRYHRIILMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIGLPPLYKLKQGKSELYLKDDA
ALNAYLASNAVEGAALIPATDEPPITGEALEKLLLLFTSANEAIARTAHRYDPALLTALIDLPPLDVETLQAEGNQHPAL
DALQAVLNRGTLGTARYQLRFDPATENAPATLVAIRRHMGEEFTQVLPMGAFESGELRPLREVSLALHDLVREGAQIVRG
NKSHSITSFAQAHAWLLDEAKKGRQVQRFKGLGEMNAEQLWETTVNPDTRRLLQVRIEDAVAADQIFSTLMGDVVEPRRD
FIEDNALKVSNLDI

Sequences:

>Translated_814_residues
MTDEQNTPPTPNGTYDSSKITVLRGLEAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDEALAGHADDIVAKILVDGSVA
VSDNGRGVPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEHLWLDIWRDGFHYQQEYALGEP
QYPLKQLEASTKRGTTLRFKPSAAIFSDVEFHYDILARRLRELSFLNSGVKITLIDERGEGRRDDFHYEGGIRSFVEHLA
QLKSPLHPNVISVTGEHNGIVVDVALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAALTRVLGTYIEQNGIAKQAKVAL
TGDDMREGMIAVLSVKVPDPSFSSQTKEKLVSSDVRPAVENAFGARLQEFLQENPNEAKAITGKIVDAARAREAARKARD
LTRRKGALDIAGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLASDQVGTL
ITALGTGIGRDEYNPDKLRYHRIILMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIGLPPLYKLKQGKSELYLKDDA
ALNAYLASNAVEGAALIPATDEPPITGEALEKLLLLFTSANEAIARTAHRYDPALLTALIDLPPLDVETLQAEGNQHPAL
DALQAVLNRGTLGTARYQLRFDPATENAPATLVAIRRHMGEEFTQVLPMGAFESGELRPLREVSLALHDLVREGAQIVRG
NKSHSITSFAQAHAWLLDEAKKGRQVQRFKGLGEMNAEQLWETTVNPDTRRLLQVRIEDAVAADQIFSTLMGDVVEPRRD
FIEDNALKVSNLDI
>Mature_813_residues
TDEQNTPPTPNGTYDSSKITVLRGLEAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDEALAGHADDIVAKILVDGSVAV
SDNGRGVPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEHLWLDIWRDGFHYQQEYALGEPQ
YPLKQLEASTKRGTTLRFKPSAAIFSDVEFHYDILARRLRELSFLNSGVKITLIDERGEGRRDDFHYEGGIRSFVEHLAQ
LKSPLHPNVISVTGEHNGIVVDVALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAALTRVLGTYIEQNGIAKQAKVALT
GDDMREGMIAVLSVKVPDPSFSSQTKEKLVSSDVRPAVENAFGARLQEFLQENPNEAKAITGKIVDAARAREAARKARDL
TRRKGALDIAGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLASDQVGTLI
TALGTGIGRDEYNPDKLRYHRIILMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIGLPPLYKLKQGKSELYLKDDAA
LNAYLASNAVEGAALIPATDEPPITGEALEKLLLLFTSANEAIARTAHRYDPALLTALIDLPPLDVETLQAEGNQHPALD
ALQAVLNRGTLGTARYQLRFDPATENAPATLVAIRRHMGEEFTQVLPMGAFESGELRPLREVSLALHDLVREGAQIVRGN
KSHSITSFAQAHAWLLDEAKKGRQVQRFKGLGEMNAEQLWETTVNPDTRRLLQVRIEDAVAADQIFSTLMGDVVEPRRDF
IEDNALKVSNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=595, Percent_Identity=26.7226890756303, Blast_Score=151, Evalue=3e-36,
Organism=Homo sapiens, GI19913406, Length=571, Percent_Identity=25.0437828371278, Blast_Score=136, Evalue=9e-32,
Organism=Escherichia coli, GI48994957, Length=808, Percent_Identity=61.3861386138614, Blast_Score=967, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=547, Percent_Identity=40.5850091407678, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535065, Length=584, Percent_Identity=25.8561643835616, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=514, Percent_Identity=24.7081712062257, Blast_Score=117, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=154, Percent_Identity=32.4675324675325, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=585, Percent_Identity=25.4700854700855, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17136538, Length=581, Percent_Identity=24.4406196213425, Blast_Score=135, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89512; Mature: 89380

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDEQNTPPTPNGTYDSSKITVLRGLEAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHH
ALAGHADDIVAKILVDGSVAVSDNGRGVPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYK
HHCCCHHHHHHHHHCCCCEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEE
VSGGLHGVGVSVVNALSEHLWLDIWRDGFHYQQEYALGEPQYPLKQLEASTKRGTTLRFK
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHCCCCEEEEC
PSAAIFSDVEFHYDILARRLRELSFLNSGVKITLIDERGEGRRDDFHYEGGIRSFVEHLA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
QLKSPLHPNVISVTGEHNGIVVDVALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAALT
HHHCCCCCCEEEEECCCCCEEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
RVLGTYIEQNGIAKQAKVALTGDDMREGMIAVLSVKVPDPSFSSQTKEKLVSSDVRPAVE
HHHHHHHHHCCCCCCEEEEECCCHHHCCEEEEEEEECCCCCCCHHHHHHHHHHCCCHHHH
NAFGARLQEFLQENPNEAKAITGKIVDAARAREAARKARDLTRRKGALDIAGLPGKLADC
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
QEKDPALSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLASDQVGTL
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHH
ITALGTGIGRDEYNPDKLRYHRIILMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIG
HHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEE
LPPLYKLKQGKSELYLKDDAALNAYLASNAVEGAALIPATDEPPITGEALEKLLLLFTSA
CCCHHHHHCCCCCEEEECCHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCC
NEAIARTAHRYDPALLTALIDLPPLDVETLQAEGNQHPALDALQAVLNRGTLGTARYQLR
CHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEE
FDPATENAPATLVAIRRHMGEEFTQVLPMGAFESGELRPLREVSLALHDLVREGAQIVRG
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
NKSHSITSFAQAHAWLLDEAKKGRQVQRFKGLGEMNAEQLWETTVNPDTRRLLQVRIEDA
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHH
VAADQIFSTLMGDVVEPRRDFIEDNALKVSNLDI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCC
>Mature Secondary Structure 
TDEQNTPPTPNGTYDSSKITVLRGLEAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHH
ALAGHADDIVAKILVDGSVAVSDNGRGVPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYK
HHCCCHHHHHHHHHCCCCEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEE
VSGGLHGVGVSVVNALSEHLWLDIWRDGFHYQQEYALGEPQYPLKQLEASTKRGTTLRFK
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHCCCCEEEEC
PSAAIFSDVEFHYDILARRLRELSFLNSGVKITLIDERGEGRRDDFHYEGGIRSFVEHLA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
QLKSPLHPNVISVTGEHNGIVVDVALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAALT
HHHCCCCCCEEEEECCCCCEEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
RVLGTYIEQNGIAKQAKVALTGDDMREGMIAVLSVKVPDPSFSSQTKEKLVSSDVRPAVE
HHHHHHHHHCCCCCCEEEEECCCHHHCCEEEEEEEECCCCCCCHHHHHHHHHHCCCHHHH
NAFGARLQEFLQENPNEAKAITGKIVDAARAREAARKARDLTRRKGALDIAGLPGKLADC
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
QEKDPALSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLASDQVGTL
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHH
ITALGTGIGRDEYNPDKLRYHRIILMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIG
HHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEE
LPPLYKLKQGKSELYLKDDAALNAYLASNAVEGAALIPATDEPPITGEALEKLLLLFTSA
CCCHHHHHCCCCCEEEECCHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCC
NEAIARTAHRYDPALLTALIDLPPLDVETLQAEGNQHPALDALQAVLNRGTLGTARYQLR
CHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEE
FDPATENAPATLVAIRRHMGEEFTQVLPMGAFESGELRPLREVSLALHDLVREGAQIVRG
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
NKSHSITSFAQAHAWLLDEAKKGRQVQRFKGLGEMNAEQLWETTVNPDTRRLLQVRIEDA
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHH
VAADQIFSTLMGDVVEPRRDFIEDNALKVSNLDI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]