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The map label for this gene is rumA [H]

Identifier: 162319803

GI number: 162319803

Start: 1939080

End: 1940414

Strand: Direct

Name: rumA [H]

Synonym: XOO_1755

Alternate gene names: 162319803

Gene position: 1939080-1940414 (Clockwise)

Preceding gene: 84623410

Following gene: 84623413

Centisome position: 39.25

GC content: 64.94

Gene sequence:

>1335_bases
GTGGCCAGAACCAGAAACCGTTTCGACCGTACCCCCTTCCAGACCGCGATCACCGACCTGAGCCATGACGGCCGCGGAGT
GGCGCGTCGCGACGGCGAGGGCGGCAAGGTCACCTTCATCAGCGGCGCCTTGCCGGGCGAGTTGGTGCGTGCCGAACCCA
CCGCCCGCAGTCGCCATTTCGATGAGGCCAAGACAGTGGAGGTGCTGGAGGCATCCCCGCAGCGCGTCGCCCCGCGCTGT
CCGCATTTCGGCGTTTGCGCCGGTTGCGTGTTGCAGCATCTTGAGGAATCGCAGCAGATCGTGGCCAAGCAGCGCGTGCT
GATGGATAACCTTGAGCGCATCGGTCATGTCACCCCGCAGGCCGTGCTGCCTGGGCTGACCGGCGACAACTGGGGCTATC
GGCGCAAGGGCCGGTTCTCGGTGCGCCGCGTGGAGAAGAAGGCCAAGACCCTGGTTGGCTTTCGCGAGCTCGACCCGCGG
TTCGTGGCCGATTTGTCCGTCTGCTACACGGTGATCCCGCAGATCGGCGAGAAGATCCCGTTGCTGGCCGCGCTGGTCGA
AGGCATGGACGGCAAGCGCGACATTCCGCAGATCGAATTCATTGCCGGCGACGATGCGGTGGCATTGACCATTCGGCATA
TGCAGCCGCTCAGCGCGCGCGACCAACAGGCCTGGATCGCATTTGCGCAGGAGCACGGGTTTGCGATCTTCCTGCAGCCT
GGCGGCGTGGACAGCGTGCATCCGCTGTGGCCGCAGGAAGTGCCTTTGTCGTTCCGTTTGCCGCAATGGGATGTGGACCT
GGCGTTCCGGCCGCTGGACTTCATCCAGGTCAACGCCTCGCTCAATCAAAAGATGATCGTGCACGCGCTGGCGCTGCTGG
ATGCCAAGCCCGACGACCGTGTGCTTGACCTGTTCTGTGGCCTGGGCAATTTCACCCTGCCGTTGGCGCGCGTGGTGCGC
GAGGTCGTGGGCGTGGAAGGCGATGCCGGGCTGGTGGCGCGGGCCAAAGACAATGCCCAGCGCAATGGTCTGGACAATGC
GCAGTTCTACGCCGCCGACCTGACCCAGGATCAGCGCAACGCCGCCTGGATGCGCCAGGGCTTCGACAAGCTGTTGCTGG
ACCCGCCACGCTCCGGCGCCCTGGAAGTGCTGCAGCAGTTACCGCTGAAGACGTTCGAGCGCATCGTCTACGTAAGCTGC
CATCCAGGCTCGCTCGCGCGCGACGCCGGCTACCTGGTCAACGAGCAAGGCTTCACGCTGGTCTCGGCTGGCGCGATGGA
CATGTTCCCGCATACCGCCCACGTGGAAAGCATTGCGGTGTTCGAGAGGCGGTGA

Upstream 100 bases:

>100_bases
TGGGTTTGCTAGGCGCGACAACTGTTGCGCTGCCCAGCGCGGCCTCCGTCGATCGCATCTGCGACAATACACCCCCTTTT
TGCCCGCAGCTTGTCGCCAC

Downstream 100 bases:

>100_bases
TTGGGGTTTCGGCATTGGGGATTCGCAAGAGCGCACCCGTTGCCTTCCAATCGCCGCTGTCGCGACGCGCGTTCTTGAAT
CCGACTTCAAAACGCGCTCC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MARTRNRFDRTPFQTAITDLSHDGRGVARRDGEGGKVTFISGALPGELVRAEPTARSRHFDEAKTVEVLEASPQRVAPRC
PHFGVCAGCVLQHLEESQQIVAKQRVLMDNLERIGHVTPQAVLPGLTGDNWGYRRKGRFSVRRVEKKAKTLVGFRELDPR
FVADLSVCYTVIPQIGEKIPLLAALVEGMDGKRDIPQIEFIAGDDAVALTIRHMQPLSARDQQAWIAFAQEHGFAIFLQP
GGVDSVHPLWPQEVPLSFRLPQWDVDLAFRPLDFIQVNASLNQKMIVHALALLDAKPDDRVLDLFCGLGNFTLPLARVVR
EVVGVEGDAGLVARAKDNAQRNGLDNAQFYAADLTQDQRNAAWMRQGFDKLLLDPPRSGALEVLQQLPLKTFERIVYVSC
HPGSLARDAGYLVNEQGFTLVSAGAMDMFPHTAHVESIAVFERR

Sequences:

>Translated_444_residues
MARTRNRFDRTPFQTAITDLSHDGRGVARRDGEGGKVTFISGALPGELVRAEPTARSRHFDEAKTVEVLEASPQRVAPRC
PHFGVCAGCVLQHLEESQQIVAKQRVLMDNLERIGHVTPQAVLPGLTGDNWGYRRKGRFSVRRVEKKAKTLVGFRELDPR
FVADLSVCYTVIPQIGEKIPLLAALVEGMDGKRDIPQIEFIAGDDAVALTIRHMQPLSARDQQAWIAFAQEHGFAIFLQP
GGVDSVHPLWPQEVPLSFRLPQWDVDLAFRPLDFIQVNASLNQKMIVHALALLDAKPDDRVLDLFCGLGNFTLPLARVVR
EVVGVEGDAGLVARAKDNAQRNGLDNAQFYAADLTQDQRNAAWMRQGFDKLLLDPPRSGALEVLQQLPLKTFERIVYVSC
HPGSLARDAGYLVNEQGFTLVSAGAMDMFPHTAHVESIAVFERR
>Mature_443_residues
ARTRNRFDRTPFQTAITDLSHDGRGVARRDGEGGKVTFISGALPGELVRAEPTARSRHFDEAKTVEVLEASPQRVAPRCP
HFGVCAGCVLQHLEESQQIVAKQRVLMDNLERIGHVTPQAVLPGLTGDNWGYRRKGRFSVRRVEKKAKTLVGFRELDPRF
VADLSVCYTVIPQIGEKIPLLAALVEGMDGKRDIPQIEFIAGDDAVALTIRHMQPLSARDQQAWIAFAQEHGFAIFLQPG
GVDSVHPLWPQEVPLSFRLPQWDVDLAFRPLDFIQVNASLNQKMIVHALALLDAKPDDRVLDLFCGLGNFTLPLARVVRE
VVGVEGDAGLVARAKDNAQRNGLDNAQFYAADLTQDQRNAAWMRQGFDKLLLDPPRSGALEVLQQLPLKTFERIVYVSCH
PGSLARDAGYLVNEQGFTLVSAGAMDMFPHTAHVESIAVFERR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=192, Percent_Identity=35.9375, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI51173878, Length=192, Percent_Identity=35.9375, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1789148, Length=448, Percent_Identity=37.9464285714286, Blast_Score=308, Evalue=6e-85,
Organism=Escherichia coli, GI1790403, Length=192, Percent_Identity=32.2916666666667, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787083, Length=182, Percent_Identity=32.4175824175824, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24666579, Length=197, Percent_Identity=34.5177664974619, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 49259; Mature: 49128

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTRNRFDRTPFQTAITDLSHDGRGVARRDGEGGKVTFISGALPGELVRAEPTARSRHF
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCHHHCCC
DEAKTVEVLEASPQRVAPRCPHFGVCAGCVLQHLEESQQIVAKQRVLMDNLERIGHVTPQ
CHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AVLPGLTGDNWGYRRKGRFSVRRVEKKAKTLVGFRELDPRFVADLSVCYTVIPQIGEKIP
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHH
LLAALVEGMDGKRDIPQIEFIAGDDAVALTIRHMQPLSARDQQAWIAFAQEHGFAIFLQP
HHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCHHHHEEEECCCEEEEECC
GGVDSVHPLWPQEVPLSFRLPQWDVDLAFRPLDFIQVNASLNQKMIVHALALLDAKPDDR
CCCCCCCCCCCCCCCCEEECCCCCCCEEECCCEEEEECCCCCHHHHHHHHHHHCCCCCHH
VLDLFCGLGNFTLPLARVVREVVGVEGDAGLVARAKDNAQRNGLDNAQFYAADLTQDQRN
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHCCCCCCEEEEECCCCCHHH
AAWMRQGFDKLLLDPPRSGALEVLQQLPLKTFERIVYVSCHPGSLARDAGYLVNEQGFTL
HHHHHHCCHHHHCCCCCCCHHHHHHHCCHHHHCEEEEEEECCCCCHHCCCCEECCCCCEE
VSAGAMDMFPHTAHVESIAVFERR
EECCCHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
ARTRNRFDRTPFQTAITDLSHDGRGVARRDGEGGKVTFISGALPGELVRAEPTARSRHF
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCHHHCCC
DEAKTVEVLEASPQRVAPRCPHFGVCAGCVLQHLEESQQIVAKQRVLMDNLERIGHVTPQ
CHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AVLPGLTGDNWGYRRKGRFSVRRVEKKAKTLVGFRELDPRFVADLSVCYTVIPQIGEKIP
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHH
LLAALVEGMDGKRDIPQIEFIAGDDAVALTIRHMQPLSARDQQAWIAFAQEHGFAIFLQP
HHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCHHHHEEEECCCEEEEECC
GGVDSVHPLWPQEVPLSFRLPQWDVDLAFRPLDFIQVNASLNQKMIVHALALLDAKPDDR
CCCCCCCCCCCCCCCCEEECCCCCCCEEECCCEEEEECCCCCHHHHHHHHHHHCCCCCHH
VLDLFCGLGNFTLPLARVVREVVGVEGDAGLVARAKDNAQRNGLDNAQFYAADLTQDQRN
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHCCCCCCEEEEECCCCCHHH
AAWMRQGFDKLLLDPPRSGALEVLQQLPLKTFERIVYVSCHPGSLARDAGYLVNEQGFTL
HHHHHHCCHHHHCCCCCCCHHHHHHHCCHHHHCEEEEEEECCCCCHHCCCCEECCCCCEE
VSAGAMDMFPHTAHVESIAVFERR
EECCCHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]