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The map label for this gene is radA

Identifier: 84490269

GI number: 84490269

Start: 1671474

End: 1672409

Strand: Direct

Name: radA

Synonym: Msp_1488

Alternate gene names: 84490269

Gene position: 1671474-1672409 (Clockwise)

Preceding gene: 84490268

Following gene: 84490270

Centisome position: 94.57

GC content: 33.76

Gene sequence:

>936_bases
ATGACAGAATTAGAAGATTTACCAAGTGTAGGAGAAAAAACAGCACAAAAACTAAGAGATGCTGGATTTGCAGATATGAT
GAGATTAGCAACTGCAACACCTAAAGAACTTAGTGTAAAAGTAGAAATTGGTGAAGGAGTAGCTGCAAAAGTAATTGAAG
CTGCAAGAAAAGCTGAAAAAATAGATTTTGAAACTGCATTCGAAGTAATGGAAAGACGTGAAGATGTTGGTAGAATAACA
ACAGGAAGTAAAGGTCTTGATGAACTTATAGGAGGAGGAATAGAAACCCAATCTATTACTGAAGTATATGGTGAATTTGG
ATCTGGAAAAAGTCAAATATCACATGAATTATCTGTTACAACCCAGTTACCTGTAGAAGAAGGTGGTCTTGATGGTGAAG
TAGTATTTATTGATACTGAAAACACGTTTAGACCTGAAAGGATAGAACAAATAGCTGAAGGATTTGGATTAAATATAGAA
GAAGTTCTTAAAAAAATTCATGTTGCAAGAGCATTTAACAGTTCACATCAAATTTTAATGGCTGATAAAATCAATGAATT
AATACAAAGTGGAGTTAATATAAAACTTATAATAATTGATTCATTAATGGCACACTTCAGAGCAGAATATGTTGGTCGTG
AATCATTAGCAACACGTCAACAAAAGTTAAATCAACACCTACACACCCTACAAACAATTGCAAACACGTATAATGTAGCA
GTTCTTATAACAAACCAAGTACAATCAAAACCAGATTCATTCTTTGGAACTCCAACAAAAGCTGTTGGTGGACACGTTTT
AGGACACGCATCAACATACCGCATATTACTTAAAAAAGGATTATCCGGTAAAAGAATAGCACGTCTTGTAGATAGTCCTC
ATTTACCTGAAGGCGAATCTGTATTTAAAGTAACTACCGAAGGTTTAGTAGATTAA

Upstream 100 bases:

>100_bases
GCTAATGCAAACTTTAATGAATATGATGAAGATATTAGGTTAAATGTAAGACAACTAGTAATAGTAGTATAATGATATGA
AAATAACAAGGAGAATTTAA

Downstream 100 bases:

>100_bases
ATACATCTACTACATTTTTTTATAGATATAATAGAATGTGCAACAATTCTGTTTTTGGTGTTTTCAATAAGTTTCTTGTT
ACCTTATTTTATAATTATTA

Product: DNA repair and recombination protein RadA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTELEDLPSVGEKTAQKLRDAGFADMMRLATATPKELSVKVEIGEGVAAKVIEAARKAEKIDFETAFEVMERREDVGRIT
TGSKGLDELIGGGIETQSITEVYGEFGSGKSQISHELSVTTQLPVEEGGLDGEVVFIDTENTFRPERIEQIAEGFGLNIE
EVLKKIHVARAFNSSHQILMADKINELIQSGVNIKLIIIDSLMAHFRAEYVGRESLATRQQKLNQHLHTLQTIANTYNVA
VLITNQVQSKPDSFFGTPTKAVGGHVLGHASTYRILLKKGLSGKRIARLVDSPHLPEGESVFKVTTEGLVD

Sequences:

>Translated_311_residues
MTELEDLPSVGEKTAQKLRDAGFADMMRLATATPKELSVKVEIGEGVAAKVIEAARKAEKIDFETAFEVMERREDVGRIT
TGSKGLDELIGGGIETQSITEVYGEFGSGKSQISHELSVTTQLPVEEGGLDGEVVFIDTENTFRPERIEQIAEGFGLNIE
EVLKKIHVARAFNSSHQILMADKINELIQSGVNIKLIIIDSLMAHFRAEYVGRESLATRQQKLNQHLHTLQTIANTYNVA
VLITNQVQSKPDSFFGTPTKAVGGHVLGHASTYRILLKKGLSGKRIARLVDSPHLPEGESVFKVTTEGLVD
>Mature_310_residues
TELEDLPSVGEKTAQKLRDAGFADMMRLATATPKELSVKVEIGEGVAAKVIEAARKAEKIDFETAFEVMERREDVGRITT
GSKGLDELIGGGIETQSITEVYGEFGSGKSQISHELSVTTQLPVEEGGLDGEVVFIDTENTFRPERIEQIAEGFGLNIEE
VLKKIHVARAFNSSHQILMADKINELIQSGVNIKLIIIDSLMAHFRAEYVGRESLATRQQKLNQHLHTLQTIANTYNVAV
LITNQVQSKPDSFFGTPTKAVGGHVLGHASTYRILLKKGLSGKRIARLVDSPHLPEGESVFKVTTEGLVD

Specific function: Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family

Homologues:

Organism=Homo sapiens, GI23238219, Length=300, Percent_Identity=45.3333333333333, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI19924133, Length=300, Percent_Identity=44, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI256017143, Length=302, Percent_Identity=39.4039735099338, Blast_Score=211, Evalue=8e-55,
Organism=Homo sapiens, GI256017141, Length=302, Percent_Identity=39.4039735099338, Blast_Score=211, Evalue=8e-55,
Organism=Homo sapiens, GI256017145, Length=221, Percent_Identity=44.3438914027149, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI17402896, Length=328, Percent_Identity=26.8292682926829, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI19924117, Length=289, Percent_Identity=29.0657439446367, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI46255039, Length=289, Percent_Identity=29.0657439446367, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI10835029, Length=289, Percent_Identity=29.0657439446367, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI153946430, Length=277, Percent_Identity=25.9927797833935, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI4885659, Length=277, Percent_Identity=25.9927797833935, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI153946427, Length=277, Percent_Identity=25.9927797833935, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI4885657, Length=170, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71997295, Length=299, Percent_Identity=42.809364548495, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI71997301, Length=299, Percent_Identity=42.809364548495, Blast_Score=242, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6321027, Length=300, Percent_Identity=47.6666666666667, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6320942, Length=299, Percent_Identity=41.1371237458194, Blast_Score=221, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6320207, Length=236, Percent_Identity=32.2033898305085, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17864108, Length=311, Percent_Identity=41.4790996784566, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24651285, Length=272, Percent_Identity=44.1176470588235, Blast_Score=227, Evalue=7e-60,
Organism=Drosophila melanogaster, GI17136506, Length=250, Percent_Identity=29.2, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24650551, Length=139, Percent_Identity=33.0935251798561, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RADA_METST (Q2NE95)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_448501.1
- HSSP:   O73948
- ProteinModelPortal:   Q2NE95
- SMR:   Q2NE95
- STRING:   Q2NE95
- GeneID:   3854698
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_1488
- NMPDR:   fig|339860.6.peg.1423
- eggNOG:   arNOG04716
- HOGENOM:   HBG413235
- OMA:   VMERRED
- PhylomeDB:   Q2NE95
- ProtClustDB:   PRK04301
- BioCyc:   MSTA339860:MSP_1488-MONOMER
- HAMAP:   MF_00348
- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011938
- InterPro:   IPR016467
- InterPro:   IPR020588
- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR020587
- PIRSF:   PIRSF005856
- SMART:   SM00382
- SMART:   SM00278
- TIGRFAMs:   TIGR02236

Pfam domain/function: PF08423 Rad51; SSF47794 Rad51_N

EC number: 3.6.1.-

Molecular weight: Translated: 34037; Mature: 33906

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50162 RECA_2; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELEDLPSVGEKTAQKLRDAGFADMMRLATATPKELSVKVEIGEGVAAKVIEAARKAEK
CCCHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHH
IDFETAFEVMERREDVGRITTGSKGLDELIGGGIETQSITEVYGEFGSGKSQISHELSVT
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHEEE
TQLPVEEGGLDGEVVFIDTENTFRPERIEQIAEGFGLNIEEVLKKIHVARAFNSSHQILM
EECCHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEE
ADKINELIQSGVNIKLIIIDSLMAHFRAEYVGRESLATRQQKLNQHLHTLQTIANTYNVA
HHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCEEE
VLITNQVQSKPDSFFGTPTKAVGGHVLGHASTYRILLKKGLSGKRIARLVDSPHLPEGES
EEEECCCCCCCCCCCCCCCHHHCCHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
VFKVTTEGLVD
EEEEECCCCCC
>Mature Secondary Structure 
TELEDLPSVGEKTAQKLRDAGFADMMRLATATPKELSVKVEIGEGVAAKVIEAARKAEK
CCHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHH
IDFETAFEVMERREDVGRITTGSKGLDELIGGGIETQSITEVYGEFGSGKSQISHELSVT
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHEEE
TQLPVEEGGLDGEVVFIDTENTFRPERIEQIAEGFGLNIEEVLKKIHVARAFNSSHQILM
EECCHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEE
ADKINELIQSGVNIKLIIIDSLMAHFRAEYVGRESLATRQQKLNQHLHTLQTIANTYNVA
HHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCEEE
VLITNQVQSKPDSFFGTPTKAVGGHVLGHASTYRILLKKGLSGKRIARLVDSPHLPEGES
EEEECCCCCCCCCCCCCCCHHHCCHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
VFKVTTEGLVD
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA