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The map label for this gene is gdhA

Identifier: 84490198

GI number: 84490198

Start: 1590965

End: 1592299

Strand: Direct

Name: gdhA

Synonym: Msp_1416

Alternate gene names: 84490198

Gene position: 1590965-1592299 (Clockwise)

Preceding gene: 84490197

Following gene: 84490200

Centisome position: 90.02

GC content: 34.91

Gene sequence:

>1335_bases
ATGTCTTATGTTGACGAAGTAATTAATAAAATTACAAAAGAAAACCCAGGTGAACCTGAATTTCACCAAACATTAAACGA
AGTATACAAATCTATTGAAGTAGCAGTAGAAGAAAATGAAGAACAATACAGAAAAGATGCAGTACTTGAAAGATTAGCAA
ACCCTGAAAGACAAATAAAATTCAGAGTTCCTTGGGTTGATGACGAAGGACAAGTTCAAGTAAACACAGGATACCGTGTA
CAATTCAACAGTGCAATTGGTCCATACAAAGGAGGATTACGTTTCCACCCTTCAGTAAACATAGGTATTATAAAATTCTT
AGGATTTGAACAAATATTCAAAAACTCATTAACAGGACTTCCAATTGGTGGAGGAAAAGGAGGATCCAACTTCGATCCTA
AAGGTAAATCAGACCGTGAAGTAATGAGTTTCTGTCAAAGTTTCATGAATGAATTATACAGACACATAGGTCAAGATCAA
GATGTACCTGCAGGAGATATAGGTGTAGGTGCTAGAGAAGTAGGATACTTATATGGACAATATAAAAGACTCACCAAACA
ATATGAAGGAGTATTAACTGGAAAAGGATTAACCTTTGGTGGATCTTTAGCAAGAACCGAAGCAACAGGTTACGGATTAT
TATACTTCACCAATGAAATGTTAAAAGCAAACAATGAAACATTAAAAGATAAAACTGCAACAGTATCTGGTTCAGGAAAT
GTAGCTATTTATGCAATTGAAAAAGCACAACAATTAGGTGCAAAAGTTGTAACAGCATCAGATTCAACTGGATGGGTATA
TGATCCTAAAGGTATCGATGTAGCATTACTCAAAGAAATCAAAGAAGTACAACGTGGAAGAATGAGTGATTATGCTGCAA
AAAGAGAATCTGCAGAATATCATGAAGGAAGAGGAGTATGGCAAATTAAATCTGATGTTGCTCTACCTTGTGCAACTCAA
AATGAATTAACATTAGATGATGCTAAACTTATTGTAGAAAATGATACCCTTGCAGTATCTGAAGGAGCAAACATGCCTAC
AACACTCGATGCTACAAAATATTTACAAGACAATGGAATATTATTCGGTCCTGCAAAAGCAGCAAATGCTGGTGGAGTAG
CAGTATCAGCTCTTGAAATGAGTCAAAACTCAGAAAGATTATACTGGACTTTTGATGAAGTTGACAATATGCTCAAAGAT
ATCATGACAAACATCTTCAGTAAATTAAATACAACAGCACAAAAATATGAACAAGACAAAAACTATGTTCTTGGTGCTAA
CGTAGCTGGATTTGAACGTGTAGTAGAAGCTATGCAATCACAAGGTATTGTATAG

Upstream 100 bases:

>100_bases
CACCTACTATAATTCTATAGTAAAGTGTTTACAACAGTAATATAATAGTTATTAAAAAAATAATAACAATTAGTTAAATA
AATCATGGAGATTAATGACA

Downstream 100 bases:

>100_bases
ATACAAACTTGAATTATGATTCAATTCATAATTCATTCTTTTATAAACTCCTAAAACCAAAAACTCATTTTAAAAAAAAT
AGAAAATAAAATTAATTATT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSYVDEVINKITKENPGEPEFHQTLNEVYKSIEVAVEENEEQYRKDAVLERLANPERQIKFRVPWVDDEGQVQVNTGYRV
QFNSAIGPYKGGLRFHPSVNIGIIKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDREVMSFCQSFMNELYRHIGQDQ
DVPAGDIGVGAREVGYLYGQYKRLTKQYEGVLTGKGLTFGGSLARTEATGYGLLYFTNEMLKANNETLKDKTATVSGSGN
VAIYAIEKAQQLGAKVVTASDSTGWVYDPKGIDVALLKEIKEVQRGRMSDYAAKRESAEYHEGRGVWQIKSDVALPCATQ
NELTLDDAKLIVENDTLAVSEGANMPTTLDATKYLQDNGILFGPAKAANAGGVAVSALEMSQNSERLYWTFDEVDNMLKD
IMTNIFSKLNTTAQKYEQDKNYVLGANVAGFERVVEAMQSQGIV

Sequences:

>Translated_444_residues
MSYVDEVINKITKENPGEPEFHQTLNEVYKSIEVAVEENEEQYRKDAVLERLANPERQIKFRVPWVDDEGQVQVNTGYRV
QFNSAIGPYKGGLRFHPSVNIGIIKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDREVMSFCQSFMNELYRHIGQDQ
DVPAGDIGVGAREVGYLYGQYKRLTKQYEGVLTGKGLTFGGSLARTEATGYGLLYFTNEMLKANNETLKDKTATVSGSGN
VAIYAIEKAQQLGAKVVTASDSTGWVYDPKGIDVALLKEIKEVQRGRMSDYAAKRESAEYHEGRGVWQIKSDVALPCATQ
NELTLDDAKLIVENDTLAVSEGANMPTTLDATKYLQDNGILFGPAKAANAGGVAVSALEMSQNSERLYWTFDEVDNMLKD
IMTNIFSKLNTTAQKYEQDKNYVLGANVAGFERVVEAMQSQGIV
>Mature_443_residues
SYVDEVINKITKENPGEPEFHQTLNEVYKSIEVAVEENEEQYRKDAVLERLANPERQIKFRVPWVDDEGQVQVNTGYRVQ
FNSAIGPYKGGLRFHPSVNIGIIKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDREVMSFCQSFMNELYRHIGQDQD
VPAGDIGVGAREVGYLYGQYKRLTKQYEGVLTGKGLTFGGSLARTEATGYGLLYFTNEMLKANNETLKDKTATVSGSGNV
AIYAIEKAQQLGAKVVTASDSTGWVYDPKGIDVALLKEIKEVQRGRMSDYAAKRESAEYHEGRGVWQIKSDVALPCATQN
ELTLDDAKLIVENDTLAVSEGANMPTTLDATKYLQDNGILFGPAKAANAGGVAVSALEMSQNSERLYWTFDEVDNMLKDI
MTNIFSKLNTTAQKYEQDKNYVLGANVAGFERVVEAMQSQGIV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=408, Percent_Identity=29.656862745098, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI4885281, Length=412, Percent_Identity=29.3689320388349, Blast_Score=159, Evalue=7e-39,
Organism=Escherichia coli, GI1788059, Length=444, Percent_Identity=55.8558558558559, Blast_Score=496, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17544676, Length=355, Percent_Identity=29.2957746478873, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6319256, Length=459, Percent_Identity=50.3267973856209, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324951, Length=453, Percent_Identity=49.6688741721854, Blast_Score=385, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24649283, Length=364, Percent_Identity=32.967032967033, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45549226, Length=415, Percent_Identity=29.6385542168675, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI45553475, Length=362, Percent_Identity=30.3867403314917, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24649460, Length=338, Percent_Identity=30.4733727810651, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48985; Mature: 48853

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYVDEVINKITKENPGEPEFHQTLNEVYKSIEVAVEENEEQYRKDAVLERLANPERQIK
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEE
FRVPWVDDEGQVQVNTGYRVQFNSAIGPYKGGLRFHPSVNIGIIKFLGFEQIFKNSLTGL
EECCEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCEEHHHHHCHHHHHHCCCCCC
PIGGGKGGSNFDPKGKSDREVMSFCQSFMNELYRHIGQDQDVPAGDIGVGAREVGYLYGQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
YKRLTKQYEGVLTGKGLTFGGSLARTEATGYGLLYFTNEMLKANNETLKDKTATVSGSGN
HHHHHHHHCCEEECCCEEECCCHHCCCCCCCEEEEEEHHHHHCCCCHHCCCCEEECCCCC
VAIYAIEKAQQLGAKVVTASDSTGWVYDPKGIDVALLKEIKEVQRGRMSDYAAKRESAEY
EEEEEEHHHHHCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHH
HEGRGVWQIKSDVALPCATQNELTLDDAKLIVENDTLAVSEGANMPTTLDATKYLQDNGI
HCCCCEEEECCCCCCCCCCCCCCEECCCEEEEECCEEEEECCCCCCCCCCHHHHHCCCCE
LFGPAKAANAGGVAVSALEMSQNSERLYWTFDEVDNMLKDIMTNIFSKLNTTAQKYEQDK
EECCCCCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NYVLGANVAGFERVVEAMQSQGIV
CEEEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SYVDEVINKITKENPGEPEFHQTLNEVYKSIEVAVEENEEQYRKDAVLERLANPERQIK
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEE
FRVPWVDDEGQVQVNTGYRVQFNSAIGPYKGGLRFHPSVNIGIIKFLGFEQIFKNSLTGL
EECCEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCEEHHHHHCHHHHHHCCCCCC
PIGGGKGGSNFDPKGKSDREVMSFCQSFMNELYRHIGQDQDVPAGDIGVGAREVGYLYGQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
YKRLTKQYEGVLTGKGLTFGGSLARTEATGYGLLYFTNEMLKANNETLKDKTATVSGSGN
HHHHHHHHCCEEECCCEEECCCHHCCCCCCCEEEEEEHHHHHCCCCHHCCCCEEECCCCC
VAIYAIEKAQQLGAKVVTASDSTGWVYDPKGIDVALLKEIKEVQRGRMSDYAAKRESAEY
EEEEEEHHHHHCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHH
HEGRGVWQIKSDVALPCATQNELTLDDAKLIVENDTLAVSEGANMPTTLDATKYLQDNGI
HCCCCEEEECCCCCCCCCCCCCCEECCCEEEEECCEEEEECCCCCCCCCCHHHHHCCCCE
LFGPAKAANAGGVAVSALEMSQNSERLYWTFDEVDNMLKDIMTNIFSKLNTTAQKYEQDK
EECCCCCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NYVLGANVAGFERVVEAMQSQGIV
CEEEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]