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The map label for this gene is gatE

Identifier: 84490194

GI number: 84490194

Start: 1586227

End: 1588125

Strand: Direct

Name: gatE

Synonym: Msp_1412

Alternate gene names: 84490194

Gene position: 1586227-1588125 (Clockwise)

Preceding gene: 84490193

Following gene: 84490195

Centisome position: 89.75

GC content: 30.91

Gene sequence:

>1899_bases
TTGTCTGAAGATAAATTTAATTATAAAGAATTAGGATTAATGATGGGATTAGAAATTCATCAACAACTAGATAGTAAAAC
TAAACTATTTTGTAGATGTCCAAATAGCCTAACAGATAAAAAACCTGAAAGAAAAATATTCCGTCGTCTAAGACCAACAC
AAAGTGAACTTGGTGAAATTGACAGAGCAGCATATGAAGAATCACAAAGAAATCTTCAATTTGTATATGAAGCATATAAT
CACCATACATGTCTTGTTGAAGCTGATGAAGAACCACCATCCCCTCTAAATCAGGAAGCTGTTGATATTTCAATAATTCT
TGCATCACTCATGAATATGAAAGTAGTTGATGAATTTCATACAATGAGAAAACAAGTAATTGATGGAAGTAACACCAGTG
GATTCCAAAGAACAGGAATTCTCGCTACTGATGGATATGTTGAAACAGAATATGGTGATGTTACAATTGAAACATTAGGT
CTTGAAGAAGATGCAGCAAGACGTATAGGAGAAGAAGATGGTAAAATTGTATTTAGACTTGACAGATTAGGTATTCCCTT
AGCAGAAATAACAACATCCCCTGACATGCATCATCCTCTTCAAGTAAAAGCTGTTGCATACCAATTAGGTCAAATCTTAA
GAAGTACTAGTGTAAAAAGAGGACTTGGTACAATTAGACAGGATTTGAATATTTCCATTAAAGAGGGAAGTAGAATAGAA
GTTAAAGGTGTTCAAGACCTTGATCTTATGCCAACAATTGTTGAAAATGAGGTTCAAAGACAATTAAATCTTATAGATAT
TAGTAAACAGTTACAAGAACGTGGAGCATGTGTTGAAGAAGAAATTATAGATGTTACAAAATATCTTGAAAACACTGAAT
CTAAAGTTATTCAAAATAGTCTTGCAAAGGAAAATAGTTGTATTTTAGCTATTAAATTAAGAAAATTCAATGGATTAATT
GGACGTGAAATTCAACCAGGTAAAAGATTAGGAACAGAATTTTCTGAACATGGAAAAAAAATGGGTGTTTCTGGATTATT
CCACACTGATGAATTACCAGCATATGGAATTGTTCAAGAAGAGGTAGATACACTTAGAGACATACTAGAATTAGATGAAG
ATGATGCATTTATTCTTGTTGCTGAAGAAAAAACAAAAGCACACAATGCATTACTTGAAATTATTAAACGTGCAAAACAA
TCACTTGAGGGTGTTCCTGAAGAAACAAGACGTGCACAAGATGATGGAAATACCCAGTATTTACGTCCATTACCTACTGC
TAGTAGAATGTATGTAGAAACTGATATTCCAACAGAAATAATTGAAAAAGAAAGAGTTAAAAAAATAGCTTCAAATCTTC
CAGAGTTACCTGTTGTTAAAAAAGAACGTATTGAAAAAGAATACAAACTTAGTGAAGATTTAGCTAAACAATTAGTACAA
CGTAATCGTGCAGATTTATTTGAAGAAATTAAAAAAGAATTACCAAGTATGGATTCAGTGAAAATTGCTTCTGATATTGT
ATATACTATTAAGGATTTAAAACGTGATGGTTATGATATTACTAAATTAACAAAAGATGCTCTTGTGGAAGTATTTAGTC
TTGTGGATAATGATGTGATTCCTGCAGCAGAAACAGAAGTCATATTAAAAGATGTTTGTAATGATATTTCTCCTAGAGAT
TCAGTGAAAAATAATAATCTTGAAAAACTATCAGATGATATTATAGTAGATACTATTAAGGAAATTATTGAAGAAAATAA
AAATATGATTGAACAAAGAAAAATGGGTGCTATGGGTCCTCTTATGGGTAAAGCCATGGCTAAATTTAAAGGTAAAGCTG
ATGGTCAAACTGTAAGTAATGTTATTAAAGATGAAATTTTAAATATCATCAACAATTAA

Upstream 100 bases:

>100_bases
ATCAGAAATATATGAAATAATGAAAACAAATCTTAAAGGAGAAATGGATACAACATTATCCCAAAATTACTTTATAAAAA
ACTAAAGGAGTGCTGAAAAA

Downstream 100 bases:

>100_bases
ATACATTATTTATTTTAATCTTCATTTTTACTTTTTTTTATAGAATCCATTTTTTTTAATATAAATTTAAATATAAAATA
AAAAGATATATTATTTACTA

Product: glutamyl-tRNA(Gln) amidotransferase subunit E

Products: NA

Alternate protein names: Glu-ADT subunit E [H]

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MSEDKFNYKELGLMMGLEIHQQLDSKTKLFCRCPNSLTDKKPERKIFRRLRPTQSELGEIDRAAYEESQRNLQFVYEAYN
HHTCLVEADEEPPSPLNQEAVDISIILASLMNMKVVDEFHTMRKQVIDGSNTSGFQRTGILATDGYVETEYGDVTIETLG
LEEDAARRIGEEDGKIVFRLDRLGIPLAEITTSPDMHHPLQVKAVAYQLGQILRSTSVKRGLGTIRQDLNISIKEGSRIE
VKGVQDLDLMPTIVENEVQRQLNLIDISKQLQERGACVEEEIIDVTKYLENTESKVIQNSLAKENSCILAIKLRKFNGLI
GREIQPGKRLGTEFSEHGKKMGVSGLFHTDELPAYGIVQEEVDTLRDILELDEDDAFILVAEEKTKAHNALLEIIKRAKQ
SLEGVPEETRRAQDDGNTQYLRPLPTASRMYVETDIPTEIIEKERVKKIASNLPELPVVKKERIEKEYKLSEDLAKQLVQ
RNRADLFEEIKKELPSMDSVKIASDIVYTIKDLKRDGYDITKLTKDALVEVFSLVDNDVIPAAETEVILKDVCNDISPRD
SVKNNNLEKLSDDIIVDTIKEIIEENKNMIEQRKMGAMGPLMGKAMAKFKGKADGQTVSNVIKDEILNIINN

Sequences:

>Translated_632_residues
MSEDKFNYKELGLMMGLEIHQQLDSKTKLFCRCPNSLTDKKPERKIFRRLRPTQSELGEIDRAAYEESQRNLQFVYEAYN
HHTCLVEADEEPPSPLNQEAVDISIILASLMNMKVVDEFHTMRKQVIDGSNTSGFQRTGILATDGYVETEYGDVTIETLG
LEEDAARRIGEEDGKIVFRLDRLGIPLAEITTSPDMHHPLQVKAVAYQLGQILRSTSVKRGLGTIRQDLNISIKEGSRIE
VKGVQDLDLMPTIVENEVQRQLNLIDISKQLQERGACVEEEIIDVTKYLENTESKVIQNSLAKENSCILAIKLRKFNGLI
GREIQPGKRLGTEFSEHGKKMGVSGLFHTDELPAYGIVQEEVDTLRDILELDEDDAFILVAEEKTKAHNALLEIIKRAKQ
SLEGVPEETRRAQDDGNTQYLRPLPTASRMYVETDIPTEIIEKERVKKIASNLPELPVVKKERIEKEYKLSEDLAKQLVQ
RNRADLFEEIKKELPSMDSVKIASDIVYTIKDLKRDGYDITKLTKDALVEVFSLVDNDVIPAAETEVILKDVCNDISPRD
SVKNNNLEKLSDDIIVDTIKEIIEENKNMIEQRKMGAMGPLMGKAMAKFKGKADGQTVSNVIKDEILNIINN
>Mature_631_residues
SEDKFNYKELGLMMGLEIHQQLDSKTKLFCRCPNSLTDKKPERKIFRRLRPTQSELGEIDRAAYEESQRNLQFVYEAYNH
HTCLVEADEEPPSPLNQEAVDISIILASLMNMKVVDEFHTMRKQVIDGSNTSGFQRTGILATDGYVETEYGDVTIETLGL
EEDAARRIGEEDGKIVFRLDRLGIPLAEITTSPDMHHPLQVKAVAYQLGQILRSTSVKRGLGTIRQDLNISIKEGSRIEV
KGVQDLDLMPTIVENEVQRQLNLIDISKQLQERGACVEEEIIDVTKYLENTESKVIQNSLAKENSCILAIKLRKFNGLIG
REIQPGKRLGTEFSEHGKKMGVSGLFHTDELPAYGIVQEEVDTLRDILELDEDDAFILVAEEKTKAHNALLEIIKRAKQS
LEGVPEETRRAQDDGNTQYLRPLPTASRMYVETDIPTEIIEKERVKKIASNLPELPVVKKERIEKEYKLSEDLAKQLVQR
NRADLFEEIKKELPSMDSVKIASDIVYTIKDLKRDGYDITKLTKDALVEVFSLVDNDVIPAAETEVILKDVCNDISPRDS
VKNNNLEKLSDDIIVDTIKEIIEENKNMIEQRKMGAMGPLMGKAMAKFKGKADGQTVSNVIKDEILNIINN

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG2511

COG function: function code J; Archaeal Glu-tRNAGln amidotransferase subunit E (contains GAD domain)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatE subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6319391, Length=277, Percent_Identity=25.9927797833935, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004115
- InterPro:   IPR023168
- InterPro:   IPR004414
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02938 GAD; PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 71539; Mature: 71408

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDKFNYKELGLMMGLEIHQQLDSKTKLFCRCPNSLTDKKPERKIFRRLRPTQSELGEI
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHH
DRAAYEESQRNLQFVYEAYNHHTCLVEADEEPPSPLNQEAVDISIILASLMNMKVVDEFH
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TMRKQVIDGSNTSGFQRTGILATDGYVETEYGDVTIETLGLEEDAARRIGEEDGKIVFRL
HHHHHHHCCCCCCCCCCCCEEEECCEEEECCCCEEEEEECCCHHHHHHCCCCCCCEEEEE
DRLGIPLAEITTSPDMHHPLQVKAVAYQLGQILRSTSVKRGLGTIRQDLNISIKEGSRIE
CCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEE
VKGVQDLDLMPTIVENEVQRQLNLIDISKQLQERGACVEEEIIDVTKYLENTESKVIQNS
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LAKENSCILAIKLRKFNGLIGREIQPGKRLGTEFSEHGKKMGVSGLFHTDELPAYGIVQE
HHCCCCEEEEEEEEHHCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCEECCCCCCCCHHHH
EVDTLRDILELDEDDAFILVAEEKTKAHNALLEIIKRAKQSLEGVPEETRRAQDDGNTQY
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCE
LRPLPTASRMYVETDIPTEIIEKERVKKIASNLPELPVVKKERIEKEYKLSEDLAKQLVQ
ECCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RNRADLFEEIKKELPSMDSVKIASDIVYTIKDLKRDGYDITKLTKDALVEVFSLVDNDVI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
PAAETEVILKDVCNDISPRDSVKNNNLEKLSDDIIVDTIKEIIEENKNMIEQRKMGAMGP
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LMGKAMAKFKGKADGQTVSNVIKDEILNIINN
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEDKFNYKELGLMMGLEIHQQLDSKTKLFCRCPNSLTDKKPERKIFRRLRPTQSELGEI
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHH
DRAAYEESQRNLQFVYEAYNHHTCLVEADEEPPSPLNQEAVDISIILASLMNMKVVDEFH
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TMRKQVIDGSNTSGFQRTGILATDGYVETEYGDVTIETLGLEEDAARRIGEEDGKIVFRL
HHHHHHHCCCCCCCCCCCCEEEECCEEEECCCCEEEEEECCCHHHHHHCCCCCCCEEEEE
DRLGIPLAEITTSPDMHHPLQVKAVAYQLGQILRSTSVKRGLGTIRQDLNISIKEGSRIE
CCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEE
VKGVQDLDLMPTIVENEVQRQLNLIDISKQLQERGACVEEEIIDVTKYLENTESKVIQNS
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LAKENSCILAIKLRKFNGLIGREIQPGKRLGTEFSEHGKKMGVSGLFHTDELPAYGIVQE
HHCCCCEEEEEEEEHHCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCEECCCCCCCCHHHH
EVDTLRDILELDEDDAFILVAEEKTKAHNALLEIIKRAKQSLEGVPEETRRAQDDGNTQY
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCE
LRPLPTASRMYVETDIPTEIIEKERVKKIASNLPELPVVKKERIEKEYKLSEDLAKQLVQ
ECCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RNRADLFEEIKKELPSMDSVKIASDIVYTIKDLKRDGYDITKLTKDALVEVFSLVDNDVI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
PAAETEVILKDVCNDISPRDSVKNNNLEKLSDDIIVDTIKEIIEENKNMIEQRKMGAMGP
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LMGKAMAKFKGKADGQTVSNVIKDEILNIINN
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 10993083 [H]