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The map label for this gene is rnhB

Identifier: 84490178

GI number: 84490178

Start: 1564036

End: 1564674

Strand: Direct

Name: rnhB

Synonym: Msp_1395

Alternate gene names: 84490178

Gene position: 1564036-1564674 (Clockwise)

Preceding gene: 84490174

Following gene: 84490179

Centisome position: 88.49

GC content: 27.86

Gene sequence:

>639_bases
ATGATACTTGATGATGAAAGTCAAATTTTAGGAATTGATGAAGCAGGAAGAGGTTCAGTAATAGGTCCACTAGTAATAGG
TGGAGTACTTATGAAAAAGAAAAAAATACGCTTCCTAAATAGAATTGGTGTAAAGGATTCTAAACAATTAAACATGAAAA
AAAGAACAATAATCTCTAGGAAAATAAAGAAAATAGCCCAGTTTAAAACAATAATAATTCCAGCCCATACAATAGATGAA
AAAAGAAACAACGACATAAACCTAAATGAGATTGAAACAGAAGGTATGGAAGAAATTATAAAAATAATGAAACCTAATGC
ATGTTATATTGATTGTATAGATGTACGTGAAAATAGATTTCATGATAAAATACAGAAAATAAATCCAAACATGACTGTTG
TAACAGAACATAAAGCTGATGAAACATATAAAATAGTATCAGCAGCATCCATTATTGCAAAGGTTGAAAGAGACAAACAA
CTAGAAATTATAAGACAAGAATATGGTTCAGTAGGCTCAGGATATCCAAGTGATAAAAATACAATAAACTACCTAAAAAC
AATAAAAAATAATCAATTTCCTCCAATAATAAGAAAAACATGGAAAACAATAGAAAATATAACAAAATCCACAGAATAA

Upstream 100 bases:

>100_bases
TTATATTTATAGAAAAAATAATTCAAAAAAATATTAACTATAAAAATACTTGAAGATACAACATATATATTAATAACATA
AAATAAGGATTAAAAATAAT

Downstream 100 bases:

>100_bases
TAATACAAGCTAATAATCCCTAAATATCCCATATATTAAATAAAATATGAAATTTATATAATATAAAATTAAGATATATA
CATTATATATAGAAAAAATT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MILDDESQILGIDEAGRGSVIGPLVIGGVLMKKKKIRFLNRIGVKDSKQLNMKKRTIISRKIKKIAQFKTIIIPAHTIDE
KRNNDINLNEIETEGMEEIIKIMKPNACYIDCIDVRENRFHDKIQKINPNMTVVTEHKADETYKIVSAASIIAKVERDKQ
LEIIRQEYGSVGSGYPSDKNTINYLKTIKNNQFPPIIRKTWKTIENITKSTE

Sequences:

>Translated_212_residues
MILDDESQILGIDEAGRGSVIGPLVIGGVLMKKKKIRFLNRIGVKDSKQLNMKKRTIISRKIKKIAQFKTIIIPAHTIDE
KRNNDINLNEIETEGMEEIIKIMKPNACYIDCIDVRENRFHDKIQKINPNMTVVTEHKADETYKIVSAASIIAKVERDKQ
LEIIRQEYGSVGSGYPSDKNTINYLKTIKNNQFPPIIRKTWKTIENITKSTE
>Mature_212_residues
MILDDESQILGIDEAGRGSVIGPLVIGGVLMKKKKIRFLNRIGVKDSKQLNMKKRTIISRKIKKIAQFKTIIIPAHTIDE
KRNNDINLNEIETEGMEEIIKIMKPNACYIDCIDVRENRFHDKIQKINPNMTVVTEHKADETYKIVSAASIIAKVERDKQ
LEIIRQEYGSVGSGYPSDKNTINYLKTIKNNQFPPIIRKTWKTIENITKSTE

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Homo sapiens, GI38455391, Length=222, Percent_Identity=28.3783783783784, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17536263, Length=224, Percent_Identity=31.6964285714286, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324257, Length=226, Percent_Identity=28.3185840707965, Blast_Score=62, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24580635, Length=226, Percent_Identity=34.070796460177, Blast_Score=104, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_METST (Q2NEI6)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_448410.1
- ProteinModelPortal:   Q2NEI6
- SMR:   Q2NEI6
- STRING:   Q2NEI6
- GeneID:   3855301
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_1395
- NMPDR:   fig|339860.6.peg.1334
- eggNOG:   arNOG07713
- HOGENOM:   HBG584843
- OMA:   AEHKADE
- PhylomeDB:   Q2NEI6
- ProtClustDB:   PRK00015
- BioCyc:   MSTA339860:MSP_1395-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_A
- InterPro:   IPR004649
- InterPro:   IPR001352
- InterPro:   IPR020787
- InterPro:   IPR023160
- InterPro:   IPR012337
- Gene3D:   G3DSA:1.10.10.460
- PANTHER:   PTHR10954
- TIGRFAMs:   TIGR00729

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 24280; Mature: 24280

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILDDESQILGIDEAGRGSVIGPLVIGGVLMKKKKIRFLNRIGVKDSKQLNMKKRTIISR
CCCCCCCCEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
KIKKIAQFKTIIIPAHTIDEKRNNDINLNEIETEGMEEIIKIMKPNACYIDCIDVRENRF
HHHHHHHHHEEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHH
HDKIQKINPNMTVVTEHKADETYKIVSAASIIAKVERDKQLEIIRQEYGSVGSGYPSDKN
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCH
TINYLKTIKNNQFPPIIRKTWKTIENITKSTE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MILDDESQILGIDEAGRGSVIGPLVIGGVLMKKKKIRFLNRIGVKDSKQLNMKKRTIISR
CCCCCCCCEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
KIKKIAQFKTIIIPAHTIDEKRNNDINLNEIETEGMEEIIKIMKPNACYIDCIDVRENRF
HHHHHHHHHEEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHH
HDKIQKINPNMTVVTEHKADETYKIVSAASIIAKVERDKQLEIIRQEYGSVGSGYPSDKN
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCH
TINYLKTIKNNQFPPIIRKTWKTIENITKSTE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA