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The map label for this gene is frhB

Identifier: 84490093

GI number: 84490093

Start: 1469751

End: 1470647

Strand: Direct

Name: frhB

Synonym: Msp_1305

Alternate gene names: 84490093

Gene position: 1469751-1470647 (Clockwise)

Preceding gene: 84490092

Following gene: 84490095

Centisome position: 83.16

GC content: 32.89

Gene sequence:

>897_bases
ATGATACAAGATTCAGTTCTTGGACCACATCAAGAAATTATAACAGCAAAAGCTGCAGACAACAAAGTTTTAAGTAAAGC
TCAAGATGGTGGAATTGTATCTGCAATTCTTATCTATGCATTAGAAGAAAATATTATTGATGGTACTATTGTTGCTGGAC
CTGGAGATGAACCATGGAAACCAGAACCTCTTGTTGCTACAACAAAAAAAGAAATTTTAAAAGGTGCTGGAACTAAATAT
ACCATGTGTCCTAACCTTTCGGTTATAAAAGAAGCAACACGTGAATATGGTCTTGAAAAGATTGGAACTGTTGGAACACC
TTGTCAAGTTATGGGATTACGTAAAATGCAAACTTATCCTCTTGGTGTTAGAAATGTTGTTGATAAAATAGCCCTATCCA
TTGGTATTTATTGTATGGAAAACTTCCCATATGAATCTTTAAAAACTTTCATCAATGATAAAGTCGGTGTTACACCATCA
CAAGTTACAAAAATGAACATTACTAAAGGTAAGTTATTTATAACCCATACTGGTGGTGAAGGTAAGATTCCTCTTAAAGA
AACGCATGGTTATGAACAGGCGGGATGTAATTATTGTATGGATTATGTTGCTGAGTTGTCTGATTTATCTTGTGGGTCTG
TTGGAGCAAAACCTGGATGGTCTACTATTATTAAACGTACCGATAAAGGAGCTTCTTTAATTGATAAAGCTATTAAAGAA
GGTGTTCTTGAAACAACAGAAACTAATGAAGGTAAATTTGGTTTAGAAATGCTTAGAAAATTATCTACTAATAAAAAGAA
AAAACACCAAAAACGTATTGATAAACAGATTGATTATGGTCTTAATATGCCATTTACCCATTCAAAAGATAAAAATGATC
CTCTTGAAAATAGGTAA

Upstream 100 bases:

>100_bases
GATGTGTAAAATGTGGTTTATGTTACTATCAATGTACACGTAGTTGGTTACCAATAGACCAAATGAAAAAAGAAATAGGA
TATTAAGGAGGAAATAAATT

Downstream 100 bases:

>100_bases
TCTTTTTACCCTATATATTTTTTTATTTTTTTTTACTTATTAATTTACTAATTTTATGTGTGTTATGCCATTATTTTAAG
TCTATTTTAAATTATTTTTA

Product: coenzyme F420-reducing hydrogenase subunit beta

Products: NA

Alternate protein names: 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta; FRH [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MIQDSVLGPHQEIITAKAADNKVLSKAQDGGIVSAILIYALEENIIDGTIVAGPGDEPWKPEPLVATTKKEILKGAGTKY
TMCPNLSVIKEATREYGLEKIGTVGTPCQVMGLRKMQTYPLGVRNVVDKIALSIGIYCMENFPYESLKTFINDKVGVTPS
QVTKMNITKGKLFITHTGGEGKIPLKETHGYEQAGCNYCMDYVAELSDLSCGSVGAKPGWSTIIKRTDKGASLIDKAIKE
GVLETTETNEGKFGLEMLRKLSTNKKKKHQKRIDKQIDYGLNMPFTHSKDKNDPLENR

Sequences:

>Translated_298_residues
MIQDSVLGPHQEIITAKAADNKVLSKAQDGGIVSAILIYALEENIIDGTIVAGPGDEPWKPEPLVATTKKEILKGAGTKY
TMCPNLSVIKEATREYGLEKIGTVGTPCQVMGLRKMQTYPLGVRNVVDKIALSIGIYCMENFPYESLKTFINDKVGVTPS
QVTKMNITKGKLFITHTGGEGKIPLKETHGYEQAGCNYCMDYVAELSDLSCGSVGAKPGWSTIIKRTDKGASLIDKAIKE
GVLETTETNEGKFGLEMLRKLSTNKKKKHQKRIDKQIDYGLNMPFTHSKDKNDPLENR
>Mature_298_residues
MIQDSVLGPHQEIITAKAADNKVLSKAQDGGIVSAILIYALEENIIDGTIVAGPGDEPWKPEPLVATTKKEILKGAGTKY
TMCPNLSVIKEATREYGLEKIGTVGTPCQVMGLRKMQTYPLGVRNVVDKIALSIGIYCMENFPYESLKTFINDKVGVTPS
QVTKMNITKGKLFITHTGGEGKIPLKETHGYEQAGCNYCMDYVAELSDLSCGSVGAKPGWSTIIKRTDKGASLIDKAIKE
GVLETTETNEGKFGLEMLRKLSTNKKKKHQKRIDKQIDYGLNMPFTHSKDKNDPLENR

Specific function: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen [H]

COG id: COG1035

COG function: function code C; Coenzyme F420-reducing hydrogenase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the frhB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007516
- InterPro:   IPR017679
- InterPro:   IPR007525 [H]

Pfam domain/function: PF04432 FrhB_FdhB_C; PF04422 FrhB_FdhB_N [H]

EC number: =1.12.98.1 [H]

Molecular weight: Translated: 32683; Mature: 32683

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQDSVLGPHQEIITAKAADNKVLSKAQDGGIVSAILIYALEENIIDGTIVAGPGDEPWK
CCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCC
PEPLVATTKKEILKGAGTKYTMCPNLSVIKEATREYGLEKIGTVGTPCQVMGLRKMQTYP
CCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHCCC
LGVRNVVDKIALSIGIYCMENFPYESLKTFINDKVGVTPSQVTKMNITKGKLFITHTGGE
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHEEEEECCCEEEEEEECCC
GKIPLKETHGYEQAGCNYCMDYVAELSDLSCGSVGAKPGWSTIIKRTDKGASLIDKAIKE
CCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHH
GVLETTETNEGKFGLEMLRKLSTNKKKKHQKRIDKQIDYGLNMPFTHSKDKNDPLENR
CCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIQDSVLGPHQEIITAKAADNKVLSKAQDGGIVSAILIYALEENIIDGTIVAGPGDEPWK
CCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCC
PEPLVATTKKEILKGAGTKYTMCPNLSVIKEATREYGLEKIGTVGTPCQVMGLRKMQTYP
CCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHCCC
LGVRNVVDKIALSIGIYCMENFPYESLKTFINDKVGVTPSQVTKMNITKGKLFITHTGGE
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHEEEEECCCEEEEEEECCC
GKIPLKETHGYEQAGCNYCMDYVAELSDLSCGSVGAKPGWSTIIKRTDKGASLIDKAIKE
CCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHH
GVLETTETNEGKFGLEMLRKLSTNKKKKHQKRIDKQIDYGLNMPFTHSKDKNDPLENR
CCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2207102; 9371463; 3663585 [H]