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The map label for this gene is purB

Identifier: 84490035

GI number: 84490035

Start: 1414607

End: 1415962

Strand: Direct

Name: purB

Synonym: Msp_1247

Alternate gene names: 84490035

Gene position: 1414607-1415962 (Clockwise)

Preceding gene: 84490034

Following gene: 84490036

Centisome position: 80.04

GC content: 31.12

Gene sequence:

>1356_bases
ATGGCAATACATCCAATAGAATTTAGATATGGTACTCCAGAAATGAAGGCTATATGGGAACAAGAATCAAAATTACAGAA
TATGTTAAAAGTTGAAGCCGCACTTGCAAAAGCAGAAGCTGAAATAGGCTTAATTCCAAAAGATGCTGCAGATGAAATAA
ATAAAAAAGCTACAACTGAATATGTTAAACAAGAACGTGTAGATGAAATTGAACAAGCTACACACCATGATATTGCAGCA
TTAATGAAAGCATTAGAAGAAGTATGTGAAAATGATGCTGGAGAATATGTTCACTATGGAGCAACAAGTAATGATATTAT
TGATTCAAGTCAATCAATACAACTTAAGGATTCAATTATAATTATAAGAGAAAAAGTTGTAAAACTCATTGAATTATTAA
TTGATTTAGCTCAAGAACATAAAAATACTGTAACAATTGGAAGAACACATGGACAACATGCACTTCCTACAACATATGGT
ATGAAATTTGCTATATACATAGATGAACTTACCCGTCAATTAGATAGATTAGATTTAACAAAAGAACATTTATGTGTTGG
TATGATGACTGGAGCAGTTGGTACAACAGCTGCTCTTGGTGATGTAGGTTATGATATTCATATGAGAGTATCTGAAATAT
TAGGACTTAATCCTGTACATATTTCAAACCAAGTAGTTCAAAGGGATAATCATGCAGAATATGTTATGACTCTTGCAAAT
ATATGTAGTACACTTGAAAAAATGGCATTAGAAGTTCGTAACTTACAAAGAACTGAAATAATGGAAGTTGGTGAAAAATT
CGATCCTAAAAAACAGGTAGGTAGTAGTACAATGCCACATAAAAGAAATCCCATAACTGGTGAAAGAATATGTGGTGTAT
CTAGAGTTGTAAGATCATATGTAAATGCAGCACTACAAAACAACCCATTATGGCATGAAAGAGACTTAACTAACTCCTCT
TGTGAACGTATCATATTACCAGAATCAGCTATATTAACAGATTATATATTAAATCTTTCATTAAAATTATTTGGTAAACT
TGAATTCCATGAAGAAAATATTGAACGTAACTTAAACTTAAGTAATGGATTGGTAATGGCTGAAAGATTCATGTCAAAAT
TAACACAAAAAGGTATGGGAAGACAAAGTGCATACAGCCTTGTAAGATCATGTGCAATGGAAGCTTATGCTCAAGATACT
GGACTTAAAGACATATTATCAAAACAAGAAGAAATTCTTGAACATTTAACAATGGAAGAAATTGAAGAAACAATGGATCC
TCATACATACCTTGGAAGCAGTGCTAAATTTGTTGATAATGTAATTAAATATGCAAAAAAAGCTATAAAAGAATAA

Upstream 100 bases:

>100_bases
TAATAATAAGTACTTTTTTACAATGATTTAATATTTAAAAAAAAAGTTTTAATTTTGAAATTTAAAGAGTTACTAAAAAA
AAATTAATTAACTGATTATT

Downstream 100 bases:

>100_bases
TGAAAAAATAATTTCTTATTCTTTTTTTTTAAATTATATGACTTATCAAAGTATAATTTTCTTTTTTTAGAATTTAAAAA
TGTTTTCTTTTTTAAATTAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MAIHPIEFRYGTPEMKAIWEQESKLQNMLKVEAALAKAEAEIGLIPKDAADEINKKATTEYVKQERVDEIEQATHHDIAA
LMKALEEVCENDAGEYVHYGATSNDIIDSSQSIQLKDSIIIIREKVVKLIELLIDLAQEHKNTVTIGRTHGQHALPTTYG
MKFAIYIDELTRQLDRLDLTKEHLCVGMMTGAVGTTAALGDVGYDIHMRVSEILGLNPVHISNQVVQRDNHAEYVMTLAN
ICSTLEKMALEVRNLQRTEIMEVGEKFDPKKQVGSSTMPHKRNPITGERICGVSRVVRSYVNAALQNNPLWHERDLTNSS
CERIILPESAILTDYILNLSLKLFGKLEFHEENIERNLNLSNGLVMAERFMSKLTQKGMGRQSAYSLVRSCAMEAYAQDT
GLKDILSKQEEILEHLTMEEIEETMDPHTYLGSSAKFVDNVIKYAKKAIKE

Sequences:

>Translated_451_residues
MAIHPIEFRYGTPEMKAIWEQESKLQNMLKVEAALAKAEAEIGLIPKDAADEINKKATTEYVKQERVDEIEQATHHDIAA
LMKALEEVCENDAGEYVHYGATSNDIIDSSQSIQLKDSIIIIREKVVKLIELLIDLAQEHKNTVTIGRTHGQHALPTTYG
MKFAIYIDELTRQLDRLDLTKEHLCVGMMTGAVGTTAALGDVGYDIHMRVSEILGLNPVHISNQVVQRDNHAEYVMTLAN
ICSTLEKMALEVRNLQRTEIMEVGEKFDPKKQVGSSTMPHKRNPITGERICGVSRVVRSYVNAALQNNPLWHERDLTNSS
CERIILPESAILTDYILNLSLKLFGKLEFHEENIERNLNLSNGLVMAERFMSKLTQKGMGRQSAYSLVRSCAMEAYAQDT
GLKDILSKQEEILEHLTMEEIEETMDPHTYLGSSAKFVDNVIKYAKKAIKE
>Mature_450_residues
AIHPIEFRYGTPEMKAIWEQESKLQNMLKVEAALAKAEAEIGLIPKDAADEINKKATTEYVKQERVDEIEQATHHDIAAL
MKALEEVCENDAGEYVHYGATSNDIIDSSQSIQLKDSIIIIREKVVKLIELLIDLAQEHKNTVTIGRTHGQHALPTTYGM
KFAIYIDELTRQLDRLDLTKEHLCVGMMTGAVGTTAALGDVGYDIHMRVSEILGLNPVHISNQVVQRDNHAEYVMTLANI
CSTLEKMALEVRNLQRTEIMEVGEKFDPKKQVGSSTMPHKRNPITGERICGVSRVVRSYVNAALQNNPLWHERDLTNSSC
ERIILPESAILTDYILNLSLKLFGKLEFHEENIERNLNLSNGLVMAERFMSKLTQKGMGRQSAYSLVRSCAMEAYAQDTG
LKDILSKQEEILEHLTMEEIEETMDPHTYLGSSAKFVDNVIKYAKKAIKE

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=453, Percent_Identity=27.3730684326711, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI183227688, Length=388, Percent_Identity=29.3814432989691, Blast_Score=149, Evalue=7e-36,
Organism=Escherichia coli, GI1787376, Length=472, Percent_Identity=26.0593220338983, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17508577, Length=379, Percent_Identity=28.2321899736148, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32564234, Length=322, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17553882, Length=283, Percent_Identity=25.0883392226148, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323391, Length=455, Percent_Identity=27.9120879120879, Blast_Score=176, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24647570, Length=455, Percent_Identity=27.032967032967, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24662684, Length=196, Percent_Identity=29.0816326530612, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI78710009, Length=351, Percent_Identity=25.0712250712251, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50840; Mature: 50709

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIHPIEFRYGTPEMKAIWEQESKLQNMLKVEAALAKAEAEIGLIPKDAADEINKKATTE
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
YVKQERVDEIEQATHHDIAALMKALEEVCENDAGEYVHYGATSNDIIDSSQSIQLKDSII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEHHHHH
IIREKVVKLIELLIDLAQEHKNTVTIGRTHGQHALPTTYGMKFAIYIDELTRQLDRLDLT
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCEEEEHHHHHHHHHHHHCCC
KEHLCVGMMTGAVGTTAALGDVGYDIHMRVSEILGLNPVHISNQVVQRDNHAEYVMTLAN
HHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHH
ICSTLEKMALEVRNLQRTEIMEVGEKFDPKKQVGSSTMPHKRNPITGERICGVSRVVRSY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VNAALQNNPLWHERDLTNSSCERIILPESAILTDYILNLSLKLFGKLEFHEENIERNLNL
HHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SNGLVMAERFMSKLTQKGMGRQSAYSLVRSCAMEAYAQDTGLKDILSKQEEILEHLTMEE
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
IEETMDPHTYLGSSAKFVDNVIKYAKKAIKE
HHHHCCCHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AIHPIEFRYGTPEMKAIWEQESKLQNMLKVEAALAKAEAEIGLIPKDAADEINKKATTE
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
YVKQERVDEIEQATHHDIAALMKALEEVCENDAGEYVHYGATSNDIIDSSQSIQLKDSII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEHHHHH
IIREKVVKLIELLIDLAQEHKNTVTIGRTHGQHALPTTYGMKFAIYIDELTRQLDRLDLT
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCEEEEHHHHHHHHHHHHCCC
KEHLCVGMMTGAVGTTAALGDVGYDIHMRVSEILGLNPVHISNQVVQRDNHAEYVMTLAN
HHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHH
ICSTLEKMALEVRNLQRTEIMEVGEKFDPKKQVGSSTMPHKRNPITGERICGVSRVVRSY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VNAALQNNPLWHERDLTNSSCERIILPESAILTDYILNLSLKLFGKLEFHEENIERNLNL
HHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SNGLVMAERFMSKLTQKGMGRQSAYSLVRSCAMEAYAQDTGLKDILSKQEEILEHLTMEE
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
IEETMDPHTYLGSSAKFVDNVIKYAKKAIKE
HHHHCCCHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]