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The map label for this gene is thyA

Identifier: 84490025

GI number: 84490025

Start: 1407319

End: 1407948

Strand: Direct

Name: thyA

Synonym: Msp_1237

Alternate gene names: 84490025

Gene position: 1407319-1407948 (Clockwise)

Preceding gene: 84490024

Following gene: 84490026

Centisome position: 79.63

GC content: 30.16

Gene sequence:

>630_bases
ATGGCAAAATTTATTCGTACTAATTCAATATCACAAGCATGGCTTACATGTGTTAAAAAAATAATGCAAGAAGGACATGA
AGTAACTGATGAAAGGGGAAGTTTAACTAAGGAACTTCAAAATGTAATTCTTGAAATTACAAATCCTAATGATAATACCA
TACCTGAATGTTCACCATGGCATGATGATAGACTTGAAACATATAAAAAGGAATTACTTGATCCTGATAATGAACAAGGA
TTTGTATATACATATGGTAACAGATTAAGACAGTATTTCAAAATAGATCAGATTGATACTTGTATCGAAAAATTAAATAA
TTGTCCCCAATCCCGAAGAGCTATTGCTATTACAATAGATCCAATTCAAGATAACAAGGTGGATGAAATCCCTTGTTTAC
AGGAAATAGCTTTTTTAATTAGGGATAATCAATTATATATGACAGATTTCTTTAGAAGCAATGATTGTGGTGGAGCAACA
TTTCCTAATCTTTTTGGTATAAGAGAGGTTGGTTATTATGTTGCTAAAAATACAGATACTGAACTTGTTAATATGGTTCA
TCATGCTATGAGCCTTCATATATATGAACATGACTGGGATAAATGTAGAGAAATTTTAAGAAAATATTAG

Upstream 100 bases:

>100_bases
TATACTTGATCCTGACTGTGGAATGCGTATGTTAGATAAAGATATTGCAGATGAAAAACTTGATATTTTAAAATATTTCA
GAGAAAATGGTGTGTAAACA

Downstream 100 bases:

>100_bases
TTATAATAATTTTTTTATAAAAATAGAAGAAAAATTATTAGTTAAAAAAAGCTAGATATAATATTATATTACCTATAATG
AGAAATAACTCATTATTGAT

Product: thymidylate synthase

Products: dihydrofolate; dTMP

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MAKFIRTNSISQAWLTCVKKIMQEGHEVTDERGSLTKELQNVILEITNPNDNTIPECSPWHDDRLETYKKELLDPDNEQG
FVYTYGNRLRQYFKIDQIDTCIEKLNNCPQSRRAIAITIDPIQDNKVDEIPCLQEIAFLIRDNQLYMTDFFRSNDCGGAT
FPNLFGIREVGYYVAKNTDTELVNMVHHAMSLHIYEHDWDKCREILRKY

Sequences:

>Translated_209_residues
MAKFIRTNSISQAWLTCVKKIMQEGHEVTDERGSLTKELQNVILEITNPNDNTIPECSPWHDDRLETYKKELLDPDNEQG
FVYTYGNRLRQYFKIDQIDTCIEKLNNCPQSRRAIAITIDPIQDNKVDEIPCLQEIAFLIRDNQLYMTDFFRSNDCGGAT
FPNLFGIREVGYYVAKNTDTELVNMVHHAMSLHIYEHDWDKCREILRKY
>Mature_208_residues
AKFIRTNSISQAWLTCVKKIMQEGHEVTDERGSLTKELQNVILEITNPNDNTIPECSPWHDDRLETYKKELLDPDNEQGF
VYTYGNRLRQYFKIDQIDTCIEKLNNCPQSRRAIAITIDPIQDNKVDEIPCLQEIAFLIRDNQLYMTDFFRSNDCGGATF
PNLFGIREVGYYVAKNTDTELVNMVHHAMSLHIYEHDWDKCREILRKY

Specific function: May catalyze the biosynthesis of dTMP using an unknown cosubstrate. In vitro, also catalyzes the dehalogenation of 5- bromo-deoxyuridine monophosphate (Br-dUMP) and the tritium exchange of [5-3H]deoxyuridine monophosphate ([5-3H]dUMP) [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Archaeal- type thyA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR014620
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: 2.1.1.45

Molecular weight: Translated: 24531; Mature: 24400

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFIRTNSISQAWLTCVKKIMQEGHEVTDERGSLTKELQNVILEITNPNDNTIPECSPW
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCC
HDDRLETYKKELLDPDNEQGFVYTYGNRLRQYFKIDQIDTCIEKLNNCPQSRRAIAITID
CHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
PIQDNKVDEIPCLQEIAFLIRDNQLYMTDFFRSNDCGGATFPNLFGIREVGYYVAKNTDT
CCCCCCCCCCHHHHHHHHHHHCCEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCH
ELVNMVHHAMSLHIYEHDWDKCREILRKY
HHHHHHHHHHHHHHEECCHHHHHHHHHCC
>Mature Secondary Structure 
AKFIRTNSISQAWLTCVKKIMQEGHEVTDERGSLTKELQNVILEITNPNDNTIPECSPW
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCC
HDDRLETYKKELLDPDNEQGFVYTYGNRLRQYFKIDQIDTCIEKLNNCPQSRRAIAITID
CHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
PIQDNKVDEIPCLQEIAFLIRDNQLYMTDFFRSNDCGGATFPNLFGIREVGYYVAKNTDT
CCCCCCCCCCHHHHHHHHHHHCCEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCH
ELVNMVHHAMSLHIYEHDWDKCREILRKY
HHHHHHHHHHHHHHEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,10-methylenetetrahydrofolate; dUMP

Specific reaction: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8617278; 8143733 [H]