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The map label for this gene is gspE [C]

Identifier: 84489954

GI number: 84489954

Start: 1331968

End: 1333302

Strand: Direct

Name: gspE [C]

Synonym: Msp_1162

Alternate gene names: 84489954

Gene position: 1331968-1333302 (Clockwise)

Preceding gene: 84489952

Following gene: 84489955

Centisome position: 75.36

GC content: 26.37

Gene sequence:

>1335_bases
ATGAAATACATGGAAATATATAAAACTAAAAAGGAACCACAATTAGATAAAACTGAAATACAAGAATATCAAAAAATAAA
AGAAAAAATAATACAACAAGAAATTAACAATGAAAAATCTATAATAAATAAAGATGTAATTCATGAATTAATAACTAAAA
ATACCACAAATCATAAAATTCTAAATAAATTATTACATGATATTCAAGGATATGGTAAAATAGATGACATGATAAATGAC
GATGACCTCGAGGAAATAATGATAATAGGAAGTAAAAAACCAGTATATGTTTATCATAGAGAAAAAGGAATGATGTTAAC
CAATATAAAACTAAATAATATGGAAATACGCCAAATAATTGATAAAATAGCAAATAATATCCAAAGAAAAATCAATAAAC
AAAGCCCAATACTAGATGCAAGACTAGATGATGGAAGTAGAGTAAATGCAACAATACCCCCAATTACTGCAGATGGTCCA
ACATTAACAATAAGAAAATTTAGAAAAAATCCTTATACAATCTTTGACTTAATAAATAAAAATACCTTAACATTAAATTT
AGCAGCATTTCTCTGGATAGTAATTGAAGGATTACATATTAGACCATCCAACATCATAATAGCTGGTGGAACTGGTTGTG
GAAAAACAACAACACTCAACACAATATCATCATTTATACCACAACATGAAAGAATTATAACAATAGAAGATACTCTTGAA
TTACAAATACCACACAAACATATCATTAGAACTGAAACAAAACCACCCAACATTGAAAATAAAGGTGAAATAACAATGGA
TTTACTACTAAAAAACTCCTTAAGACAACGTCCTGATAGAATAATTATAGGAGAAGTAAGATCAAACGAAGCAATAACAT
TGTTTACAGCATTAAATACTGGACATTCTGGTATGGGAACATTACATGCTAATTCAACATATGAAACAATAACACGACTA
ACAAACCCACCAATGAGCGTTCCAAACATTATGATCAAATCAATAGATTTTATAATAATGCAAAAAAGAATTTATCATCC
AAAATATGGAATTATAAGGCGAATAACTGATGTAGCTGAAGTTGTTGGAATGGAGAATAATAAAATTCAATTAAATACAA
TATACAAATACAATTCAGTGAATGATAGTATAAAATACACTGCAATAAATTGTAATGCATTGAATAAAATAGCATATCTT
ACAGGACTCACATACAAACAAGTACATGAAGAAATACATGAAAGAGAAAAATATTTAGAAAAATATCATGATAAAAACAT
GAATATAGAAAAAGTACATGAAATTATAAATAATTACCATCAAAAGACATTATGA

Upstream 100 bases:

>100_bases
TTTCATAATAATAGTATGGTATTTATTATAAAAATAATTTAGAAAATAAATTGTAATACTATTTATTAAATAAGTAATAA
ATATAATAGAGCAACACATT

Downstream 100 bases:

>100_bases
GAGAATTTATAGAATATGTGGGATTTAAAACAATAAAACTTTATTTACATATAACTAAGAAAAAGAAAATTGAAAATAAA
ATTCAAAGAGATGTTGATGA

Product: type II/IV secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKYMEIYKTKKEPQLDKTEIQEYQKIKEKIIQQEINNEKSIINKDVIHELITKNTTNHKILNKLLHDIQGYGKIDDMIND
DDLEEIMIIGSKKPVYVYHREKGMMLTNIKLNNMEIRQIIDKIANNIQRKINKQSPILDARLDDGSRVNATIPPITADGP
TLTIRKFRKNPYTIFDLINKNTLTLNLAAFLWIVIEGLHIRPSNIIIAGGTGCGKTTTLNTISSFIPQHERIITIEDTLE
LQIPHKHIIRTETKPPNIENKGEITMDLLLKNSLRQRPDRIIIGEVRSNEAITLFTALNTGHSGMGTLHANSTYETITRL
TNPPMSVPNIMIKSIDFIIMQKRIYHPKYGIIRRITDVAEVVGMENNKIQLNTIYKYNSVNDSIKYTAINCNALNKIAYL
TGLTYKQVHEEIHEREKYLEKYHDKNMNIEKVHEIINNYHQKTL

Sequences:

>Translated_444_residues
MKYMEIYKTKKEPQLDKTEIQEYQKIKEKIIQQEINNEKSIINKDVIHELITKNTTNHKILNKLLHDIQGYGKIDDMIND
DDLEEIMIIGSKKPVYVYHREKGMMLTNIKLNNMEIRQIIDKIANNIQRKINKQSPILDARLDDGSRVNATIPPITADGP
TLTIRKFRKNPYTIFDLINKNTLTLNLAAFLWIVIEGLHIRPSNIIIAGGTGCGKTTTLNTISSFIPQHERIITIEDTLE
LQIPHKHIIRTETKPPNIENKGEITMDLLLKNSLRQRPDRIIIGEVRSNEAITLFTALNTGHSGMGTLHANSTYETITRL
TNPPMSVPNIMIKSIDFIIMQKRIYHPKYGIIRRITDVAEVVGMENNKIQLNTIYKYNSVNDSIKYTAINCNALNKIAYL
TGLTYKQVHEEIHEREKYLEKYHDKNMNIEKVHEIINNYHQKTL
>Mature_444_residues
MKYMEIYKTKKEPQLDKTEIQEYQKIKEKIIQQEINNEKSIINKDVIHELITKNTTNHKILNKLLHDIQGYGKIDDMIND
DDLEEIMIIGSKKPVYVYHREKGMMLTNIKLNNMEIRQIIDKIANNIQRKINKQSPILDARLDDGSRVNATIPPITADGP
TLTIRKFRKNPYTIFDLINKNTLTLNLAAFLWIVIEGLHIRPSNIIIAGGTGCGKTTTLNTISSFIPQHERIITIEDTLE
LQIPHKHIIRTETKPPNIENKGEITMDLLLKNSLRQRPDRIIIGEVRSNEAITLFTALNTGHSGMGTLHANSTYETITRL
TNPPMSVPNIMIKSIDFIIMQKRIYHPKYGIIRRITDVAEVVGMENNKIQLNTIYKYNSVNDSIKYTAINCNALNKIAYL
TGLTYKQVHEEIHEREKYLEKYHDKNMNIEKVHEIINNYHQKTL

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=307, Percent_Identity=26.7100977198697, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786296, Length=142, Percent_Identity=34.5070422535211, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082188, Length=147, Percent_Identity=31.9727891156463, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011451
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006141
- InterPro:   IPR001482 [H]

Pfam domain/function: PF07591 DUF1557; PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 51368; Mature: 51368

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYMEIYKTKKEPQLDKTEIQEYQKIKEKIIQQEINNEKSIINKDVIHELITKNTTNHKI
CCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHH
LNKLLHDIQGYGKIDDMINDDDLEEIMIIGSKKPVYVYHREKGMMLTNIKLNNMEIRQII
HHHHHHHHCCCCCHHCCCCCCCCCEEEEECCCCCEEEEECCCCEEEEEEEECCHHHHHHH
DKIANNIQRKINKQSPILDARLDDGSRVNATIPPITADGPTLTIRKFRKNPYTIFDLINK
HHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCC
NTLTLNLAAFLWIVIEGLHIRPSNIIIAGGTGCGKTTTLNTISSFIPQHERIITIEDTLE
CEEEEHHHHHHHHHHHCCEECCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECEEE
LQIPHKHIIRTETKPPNIENKGEITMDLLLKNSLRQRPDRIIIGEVRSNEAITLFTALNT
EECCHHHHEECCCCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECC
GHSGMGTLHANSTYETITRLTNPPMSVPNIMIKSIDFIIMQKRIYHPKYGIIRRITDVAE
CCCCCEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VVGMENNKIQLNTIYKYNSVNDSIKYTAINCNALNKIAYLTGLTYKQVHEEIHEREKYLE
HHCCCCCEEEEEEEEEECCCCCCEEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KYHDKNMNIEKVHEIINNYHQKTL
HHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYMEIYKTKKEPQLDKTEIQEYQKIKEKIIQQEINNEKSIINKDVIHELITKNTTNHKI
CCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHH
LNKLLHDIQGYGKIDDMINDDDLEEIMIIGSKKPVYVYHREKGMMLTNIKLNNMEIRQII
HHHHHHHHCCCCCHHCCCCCCCCCEEEEECCCCCEEEEECCCCEEEEEEEECCHHHHHHH
DKIANNIQRKINKQSPILDARLDDGSRVNATIPPITADGPTLTIRKFRKNPYTIFDLINK
HHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCC
NTLTLNLAAFLWIVIEGLHIRPSNIIIAGGTGCGKTTTLNTISSFIPQHERIITIEDTLE
CEEEEHHHHHHHHHHHCCEECCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECEEE
LQIPHKHIIRTETKPPNIENKGEITMDLLLKNSLRQRPDRIIIGEVRSNEAITLFTALNT
EECCHHHHEECCCCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECC
GHSGMGTLHANSTYETITRLTNPPMSVPNIMIKSIDFIIMQKRIYHPKYGIIRRITDVAE
CCCCCEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VVGMENNKIQLNTIYKYNSVNDSIKYTAINCNALNKIAYLTGLTYKQVHEEIHEREKYLE
HHCCCCCEEEEEEEEEECCCCCCEEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KYHDKNMNIEKVHEIINNYHQKTL
HHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]