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The map label for this gene is leuA2

Identifier: 84489893

GI number: 84489893

Start: 1247182

End: 1248021

Strand: Direct

Name: leuA2

Synonym: Msp_1099

Alternate gene names: 84489893

Gene position: 1247182-1248021 (Clockwise)

Preceding gene: 84489892

Following gene: 84489894

Centisome position: 70.57

GC content: 29.88

Gene sequence:

>840_bases
ATGGGATTATCAGACCTACATCTAGAAGTTAAAATAAACAAACCACGAGATGTAGTAAATCAAATATGTATGGATGCAAT
AGATTATGGTAAGGATCATGGACTATTTGTAGCATTCTCTGCTGAAGATGCAACAAGAACAGAACTTCCAAAACTTCTTG
ATGTATATAAACAAGCACAAGATCATGGAGCAGACAGAATTCATATAGCAGATACAACAGGTTCAATAAATCCATATGCA
ACACAGTACTTAGTAAAAAATATTAAAAAAGAAATAGATACAGAAATAGCACTACACTGTCATAATGACTTTGGATTTGC
AGTTGCAAATTCCATTGCAGGATTATTTGAAGGCGCAACTGCAATATCTACAACAGTAAATGGTATAGGTGAAAGAGCAG
GAAATGCCTCTCTAGAAGAATTAATAATGTCATTAAAACTATTATATAATAAGGATTTAGGATTTAAAACAGAAGTAATC
TATGAACTATCACAACTTGTATCAAAATATAGTAAAATTCCAATATCAGATAGTAAAGCTATAGTTGGAAACAATGTATT
TAGACATGAATCAGGAATACATGTAGATGCAATAGTAAAAAATCCATTAGCATATGAACCATTCATTCCAGAAATGATTG
GAACAAAAAGACAAATAGTTCTAGGAAAACATTCAGGTAAATCAGCAGTAATAGAAAAATTAGACACATTAAATATAAAA
GTAGATGACACACAATTATCACAAATAGTTTCCCTAGTAAAACAAGAACGTGAAAGAGGAGAAGAAATAACAAATAATAA
ATTCGATGAAATACTAGAAAAAGTTAATATTAAAAGATGA

Upstream 100 bases:

>100_bases
TAATGAAGGACTAGATGCTCAAATAATCTGTTTAACAAGAACTAAAAAGGAAGACATTGATGCGGCATTAGATGCTGATG
TAGATGGAATAATAACATTT

Downstream 100 bases:

>100_bases
GGATTAATAATGAATATAAGTGAAAAAATACTTGCAAAAGCATCAAATAAGGAAGAAGTAAGTCCTGGAGATACAATTAC
TGCAAATATAGATGTTGCAA

Product: LeuA2

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1 [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MGLSDLHLEVKINKPRDVVNQICMDAIDYGKDHGLFVAFSAEDATRTELPKLLDVYKQAQDHGADRIHIADTTGSINPYA
TQYLVKNIKKEIDTEIALHCHNDFGFAVANSIAGLFEGATAISTTVNGIGERAGNASLEELIMSLKLLYNKDLGFKTEVI
YELSQLVSKYSKIPISDSKAIVGNNVFRHESGIHVDAIVKNPLAYEPFIPEMIGTKRQIVLGKHSGKSAVIEKLDTLNIK
VDDTQLSQIVSLVKQERERGEEITNNKFDEILEKVNIKR

Sequences:

>Translated_279_residues
MGLSDLHLEVKINKPRDVVNQICMDAIDYGKDHGLFVAFSAEDATRTELPKLLDVYKQAQDHGADRIHIADTTGSINPYA
TQYLVKNIKKEIDTEIALHCHNDFGFAVANSIAGLFEGATAISTTVNGIGERAGNASLEELIMSLKLLYNKDLGFKTEVI
YELSQLVSKYSKIPISDSKAIVGNNVFRHESGIHVDAIVKNPLAYEPFIPEMIGTKRQIVLGKHSGKSAVIEKLDTLNIK
VDDTQLSQIVSLVKQERERGEEITNNKFDEILEKVNIKR
>Mature_278_residues
GLSDLHLEVKINKPRDVVNQICMDAIDYGKDHGLFVAFSAEDATRTELPKLLDVYKQAQDHGADRIHIADTTGSINPYAT
QYLVKNIKKEIDTEIALHCHNDFGFAVANSIAGLFEGATAISTTVNGIGERAGNASLEELIMSLKLLYNKDLGFKTEVIY
ELSQLVSKYSKIPISDSKAIVGNNVFRHESGIHVDAIVKNPLAYEPFIPEMIGTKRQIVLGKHSGKSAVIEKLDTLNIKV
DDTQLSQIVSLVKQERERGEEITNNKFDEILEKVNIKR

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=260, Percent_Identity=36.5384615384615, Blast_Score=153, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320071, Length=259, Percent_Identity=31.6602316602317, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6320019, Length=259, Percent_Identity=31.6602316602317, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 30977; Mature: 30846

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLSDLHLEVKINKPRDVVNQICMDAIDYGKDHGLFVAFSAEDATRTELPKLLDVYKQAQ
CCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
DHGADRIHIADTTGSINPYATQYLVKNIKKEIDTEIALHCHNDFGFAVANSIAGLFEGAT
HCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCHH
AISTTVNGIGERAGNASLEELIMSLKLLYNKDLGFKTEVIYELSQLVSKYSKIPISDSKA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCE
IVGNNVFRHESGIHVDAIVKNPLAYEPFIPEMIGTKRQIVLGKHSGKSAVIEKLDTLNIK
ECCCCCEECCCCCEEEEEECCCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHCEEE
VDDTQLSQIVSLVKQERERGEEITNNKFDEILEKVNIKR
ECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
GLSDLHLEVKINKPRDVVNQICMDAIDYGKDHGLFVAFSAEDATRTELPKLLDVYKQAQ
CCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
DHGADRIHIADTTGSINPYATQYLVKNIKKEIDTEIALHCHNDFGFAVANSIAGLFEGAT
HCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCHH
AISTTVNGIGERAGNASLEELIMSLKLLYNKDLGFKTEVIYELSQLVSKYSKIPISDSKA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCE
IVGNNVFRHESGIHVDAIVKNPLAYEPFIPEMIGTKRQIVLGKHSGKSAVIEKLDTLNIK
ECCCCCEECCCCCEEEEEECCCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHCEEE
VDDTQLSQIVSLVKQERERGEEITNNKFDEILEKVNIKR
ECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]