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The map label for this gene is leuC2

Identifier: 84489894

GI number: 84489894

Start: 1248031

End: 1249278

Strand: Direct

Name: leuC2

Synonym: Msp_1100

Alternate gene names: 84489894

Gene position: 1248031-1249278 (Clockwise)

Preceding gene: 84489893

Following gene: 84489897

Centisome position: 70.61

GC content: 32.93

Gene sequence:

>1248_bases
ATGAATATAAGTGAAAAAATACTTGCAAAAGCATCAAATAAGGAAGAAGTAAGTCCTGGAGATACAATTACTGCAAATAT
AGATGTTGCAATGAGTCATGATGGAACAAGTCCTCCTACAATAAAAGTATTTGAAAAAATAGCTGATAAAGTATGGGATC
CAGAAAAAATAGTTCTTGTATTCGATCATGTGATTCCTGCAAACACAATCGGATCTGCTGAATTTCAACAGGTAGTAAGA
GAATTTGGTAAAAAACAAAAGATTCCAAACATGTATATTCAAGGAGAAGGAGTTTGTCATGAAGTACTTCCAGACTATGG
TCATGTAAAACCTTCAACAGTAATAGTTGGAGCAGATTCACATACATGTACATATGGTGCTTTTGGTGCATTCTCAACAG
GACTTGGAGCTACAGATCTTGCAATGGTATATGCAACTGGACAAACATGGTTTAATGTTCCAGAATCCTTAAAAATAAAT
GTTAATGGTACATTAAATGAAAATGTATACTCTAAAGATGTAATTCTAAAAATAATTAAGGAATTAGGTGCCTATGGTGC
TACTTATAAAAGTCTTGAATTCCATGGTGATACAATTGATAATATGTCTGTAGCTAGTAGATTAACAATGACAAATATGG
CAATTGAATGTGGAGCTAAAAATGGTATAATGGTACCTAATAAACAGACAAAGGAATATCTCTCACAAAGAGGAATAACT
GATTATACTATTACTACTGCTAGTAAAGATGCAGAATATGAGAAAATTTATGACTTTGATGTTGATGATCTTCAACCACA
AATTGCATGTCCACATAATGTAGATAATGTTGAAGATATAGATAAAGTAGCAGGTACTCATATAGATCAAGCAGTACTTG
GTTCATGTACCAATGGACGTTATGAAGATTTACTTCAAGCAGCTGAAGTTATTGAAGGACATAAAATACATGAAGATGTT
GAATTACTAGTATTCCCAGCATCAAGACATGTGTATGAAAAAGCAATAGAAACAGGTGTTATTCAAACATTACTTAAATC
AAATGCAATTATATGTAATCCTGGATGTGGACCATGTCTTGGAGCACATATGGGTGTAATGACAGACGATATGACTTGTA
TTTCCACTACTAATAGAAATTTCCTTGGTAGAATGGGTAGTGCTAAGTCATATGTATATCTATCAAATCCAGCAGTTGTT
GCAGCTTCAGCAATAAAAGGTGAAATTACAAATCCAAGTGAGATATAA

Upstream 100 bases:

>100_bases
TTCCCTAGTAAAACAAGAACGTGAAAGAGGAGAAGAAATAACAAATAATAAATTCGATGAAATACTAGAAAAAGTTAATA
TTAAAAGATGAGGATTAATA

Downstream 100 bases:

>100_bases
TTTCATTTTTTCCAATTCTTTTTTTGTTATATTAATTAAAAAATAGTATTTTCCAAAAAAAAAACTTATTTTATAAAAAA
AATAGATGAAAGATTAGTAT

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNISEKILAKASNKEEVSPGDTITANIDVAMSHDGTSPPTIKVFEKIADKVWDPEKIVLVFDHVIPANTIGSAEFQQVVR
EFGKKQKIPNMYIQGEGVCHEVLPDYGHVKPSTVIVGADSHTCTYGAFGAFSTGLGATDLAMVYATGQTWFNVPESLKIN
VNGTLNENVYSKDVILKIIKELGAYGATYKSLEFHGDTIDNMSVASRLTMTNMAIECGAKNGIMVPNKQTKEYLSQRGIT
DYTITTASKDAEYEKIYDFDVDDLQPQIACPHNVDNVEDIDKVAGTHIDQAVLGSCTNGRYEDLLQAAEVIEGHKIHEDV
ELLVFPASRHVYEKAIETGVIQTLLKSNAIICNPGCGPCLGAHMGVMTDDMTCISTTNRNFLGRMGSAKSYVYLSNPAVV
AASAIKGEITNPSEI

Sequences:

>Translated_415_residues
MNISEKILAKASNKEEVSPGDTITANIDVAMSHDGTSPPTIKVFEKIADKVWDPEKIVLVFDHVIPANTIGSAEFQQVVR
EFGKKQKIPNMYIQGEGVCHEVLPDYGHVKPSTVIVGADSHTCTYGAFGAFSTGLGATDLAMVYATGQTWFNVPESLKIN
VNGTLNENVYSKDVILKIIKELGAYGATYKSLEFHGDTIDNMSVASRLTMTNMAIECGAKNGIMVPNKQTKEYLSQRGIT
DYTITTASKDAEYEKIYDFDVDDLQPQIACPHNVDNVEDIDKVAGTHIDQAVLGSCTNGRYEDLLQAAEVIEGHKIHEDV
ELLVFPASRHVYEKAIETGVIQTLLKSNAIICNPGCGPCLGAHMGVMTDDMTCISTTNRNFLGRMGSAKSYVYLSNPAVV
AASAIKGEITNPSEI
>Mature_415_residues
MNISEKILAKASNKEEVSPGDTITANIDVAMSHDGTSPPTIKVFEKIADKVWDPEKIVLVFDHVIPANTIGSAEFQQVVR
EFGKKQKIPNMYIQGEGVCHEVLPDYGHVKPSTVIVGADSHTCTYGAFGAFSTGLGATDLAMVYATGQTWFNVPESLKIN
VNGTLNENVYSKDVILKIIKELGAYGATYKSLEFHGDTIDNMSVASRLTMTNMAIECGAKNGIMVPNKQTKEYLSQRGIT
DYTITTASKDAEYEKIYDFDVDDLQPQIACPHNVDNVEDIDKVAGTHIDQAVLGSCTNGRYEDLLQAAEVIEGHKIHEDV
ELLVFPASRHVYEKAIETGVIQTLLKSNAIICNPGCGPCLGAHMGVMTDDMTCISTTNRNFLGRMGSAKSYVYLSNPAVV
AASAIKGEITNPSEI

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=387, Percent_Identity=28.9405684754522, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI8659555, Length=482, Percent_Identity=23.6514522821577, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786259, Length=438, Percent_Identity=28.5388127853881, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI87081781, Length=345, Percent_Identity=25.7971014492754, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787531, Length=441, Percent_Identity=22.4489795918367, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI2367097, Length=463, Percent_Identity=24.4060475161987, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=342, Percent_Identity=27.1929824561404, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI32564738, Length=342, Percent_Identity=27.1929824561404, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=279, Percent_Identity=27.5985663082437, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17568399, Length=484, Percent_Identity=24.5867768595041, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320440, Length=447, Percent_Identity=32.8859060402685, Blast_Score=206, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6321429, Length=440, Percent_Identity=29.7727272727273, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6323335, Length=349, Percent_Identity=29.7994269340974, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6322261, Length=343, Percent_Identity=25.0728862973761, Blast_Score=123, Evalue=5e-29,
Organism=Drosophila melanogaster, GI161076999, Length=343, Percent_Identity=26.8221574344023, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI281365315, Length=343, Percent_Identity=26.8221574344023, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI17864292, Length=343, Percent_Identity=26.8221574344023, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI28571643, Length=344, Percent_Identity=27.906976744186, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI17137564, Length=491, Percent_Identity=23.0142566191446, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24645686, Length=515, Percent_Identity=23.6893203883495, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 45038; Mature: 45038

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00450 ACONITASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISEKILAKASNKEEVSPGDTITANIDVAMSHDGTSPPTIKVFEKIADKVWDPEKIVLV
CCCHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEE
FDHVIPANTIGSAEFQQVVREFGKKQKIPNMYIQGEGVCHEVLPDYGHVKPSTVIVGADS
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHCCCCCCCCCCEEEEECCC
HTCTYGAFGAFSTGLGATDLAMVYATGQTWFNVPESLKINVNGTLNENVYSKDVILKIIK
CCEECCCCHHHHCCCCCCCEEEEEEECCCEECCCCEEEEEECCEECCCCHHHHHHHHHHH
ELGAYGATYKSLEFHGDTIDNMSVASRLTMTNMAIECGAKNGIMVPNKQTKEYLSQRGIT
HHCCCCCEECEEEECCCCCCCHHHHHHHHHHHEEEEECCCCCEECCCHHHHHHHHHCCCC
DYTITTASKDAEYEKIYDFDVDDLQPQIACPHNVDNVEDIDKVAGTHIDQAVLGSCTNGR
EEEEEECCCCCCHHHEECCCHHHCCCCEECCCCCCCHHHHHHHHCCHHHHHHHCCCCCCH
YEDLLQAAEVIEGHKIHEDVELLVFPASRHVYEKAIETGVIQTLLKSNAIICNPGCGPCL
HHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHH
GAHMGVMTDDMTCISTTNRNFLGRMGSAKSYVYLSNPAVVAASAIKGEITNPSEI
HCCCCCCCCCCEEEEECCCHHHHCCCCCCEEEEECCCCEEEEHHHCCCCCCCCCC
>Mature Secondary Structure
MNISEKILAKASNKEEVSPGDTITANIDVAMSHDGTSPPTIKVFEKIADKVWDPEKIVLV
CCCHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEE
FDHVIPANTIGSAEFQQVVREFGKKQKIPNMYIQGEGVCHEVLPDYGHVKPSTVIVGADS
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHCCCCCCCCCCEEEEECCC
HTCTYGAFGAFSTGLGATDLAMVYATGQTWFNVPESLKINVNGTLNENVYSKDVILKIIK
CCEECCCCHHHHCCCCCCCEEEEEEECCCEECCCCEEEEEECCEECCCCHHHHHHHHHHH
ELGAYGATYKSLEFHGDTIDNMSVASRLTMTNMAIECGAKNGIMVPNKQTKEYLSQRGIT
HHCCCCCEECEEEECCCCCCCHHHHHHHHHHHEEEEECCCCCEECCCHHHHHHHHHCCCC
DYTITTASKDAEYEKIYDFDVDDLQPQIACPHNVDNVEDIDKVAGTHIDQAVLGSCTNGR
EEEEEECCCCCCHHHEECCCHHHCCCCEECCCCCCCHHHHHHHHCCHHHHHHHCCCCCCH
YEDLLQAAEVIEGHKIHEDVELLVFPASRHVYEKAIETGVIQTLLKSNAIICNPGCGPCL
HHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHH
GAHMGVMTDDMTCISTTNRNFLGRMGSAKSYVYLSNPAVVAASAIKGEITNPSEI
HCCCCCCCCCCEEEEECCCHHHHCCCCCCEEEEECCCCEEEEHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]