Click here to switch to the map view.

The map label for this gene is glyS

Identifier: 84489844

GI number: 84489844

Start: 1194456

End: 1196198

Strand: Direct

Name: glyS

Synonym: Msp_1049

Alternate gene names: 84489844

Gene position: 1194456-1196198 (Clockwise)

Preceding gene: 84489843

Following gene: 84489845

Centisome position: 67.58

GC content: 29.2

Gene sequence:

>1743_bases
ATGGGAAATGAAGAAGTAATGAGAATATCTAGAAAAAGAGGTTATTTATATCCATCTTTTGAAATATATTCTGGAGTAGC
AGGATTTTATGATTATGGACCACTAGGTGGTGTTCTTAAAAATAATATAATGAATCTATGGAGAAAATACTATGTTTCAA
ATGAAGGATTCTATGAAATTGAAGCACCTACCATAATGCCAAGAGAAACATTGAAGGCATCAGGACATGTTGATAACTTT
ACAGATCCTATGACACAATGTATGGAATGTAATGAAGTATATCGTGCTGATCATATCATTGAAGATGCTATTAGTAAAGA
AGTAGAAGGACTTCCAGATGAAAAATTAACAGAAATTATTGAAGAAAATAACATAGTTTGCCAAAATTGTGGAGCTAAAC
TTACACCAGTAAAAAGTTATAATTTAATGTTTAAAACAGAAATAGGTGTTAGTGGAAAACAAACTGGTTTCATGCGTCCT
GAAACTGCTCAAGGTATATTCATAGCATTTAAAAGAATTAGTAGATTTTATAAAGATAAGCTTCCATTTGGTATAGTACA
GTTAGGTAAATCATATAGAAATGAACTTTCACCAAGACAGGGAGTTATTAGATTAAGAGAATTTACACAAGCAGAAGCTG
AAATATTTGTAGATCCATCTGATAAAACACATAAAAATTATAAAAATATAGAAAATCATGTACTACATCTATATAATCAA
AAAGAACAATTGAATGAAGGAAAACCTATTGAAATAACAGTAAAAGACGCCATAGATAATAACATTGTTTCAAGTGAAAT
GTTAATTTATCAAATAGTTATTGCCCATAAATTCTTAAGAGACCTTGGAATTGAAGATGATGTTATTCGATTTAGACAAC
ATTTACCTGATGAAATGGCTCATTATGCAATTGATTGTTGGGATGCTGAAGTAAAAACTGATCAATATGGATGGGTTGAA
ATAATAGGTATTGCCGATCGTACAGATTTTGATTTAAAATCCCATATAAAACATAGTAAAGAGGATTTATCAGTGTTTAA
AGAATATGATACACCAAAAAATGTAACTGTTACAAAACCTTCATTTAATATGAAAAAATTTGGTCCAACATTTAAAGGAG
ATTCACCAAAAGCTAAAGAAATTCTTGAAAATACTGATAGTAATATTATTAAAGAATCATTTGACAAAGATGGAGTATTT
TCTTTTAGTATTGGAGAAAATAAATATGAAATCGACCAATCCTTTGTTAAATTTGAAACTAAAGAAGAACAAATACGTGG
AGATCGTATTATCCCTCATGTTATAGAACCATCATATGGTATTGATCGTATAATCTATTCAACATTACTTCATTCATATA
CTGAAGATGAAACAGAAGAAGGTGAAAAAAGATCATATCTTAAATTACCTGCTAAAATTGCTCCTATTAAAGCAGCAATC
CTCCCACTTGTAAATAAACAACCACTAACTCTTATTGCAGAGGATATAGAAACAACACTTCGATCTAGTAATATAATTAC
ATCTTTTGATGGAAGTGGAACTATTGGTCGTAGATATGCTAGAGCAGATGAAATAGGTATTCCATATGCTATAACAATAG
ATTATGATACTGTTGATGATAAGAAAGTTACTATACGTAATAGGGATACCTTTAAACAAAAACGTGTGGAAATAGAAAAA
CTTTCAATGATAATAAATAACTTAGTAGAAGGACATATTAACTTTGAAGATATTGAAGAATAG

Upstream 100 bases:

>100_bases
CATATGGCCATGAAGATAGAGATAGTAAATATATGGGACAAACTGGACCACAAGTAAGTAAAATCAAGGAAGATTTTGAT
ATAATAAATGGAGGCAAATA

Downstream 100 bases:

>100_bases
GTGATTATATATGATTATAGATGCCCATGTACATGCAGATTGTAGGCCTATTGAGGACTTTAGAGATATTAAAATAGGTG
GTGTTAATGCAATAATTACA

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS [H]

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MGNEEVMRISRKRGYLYPSFEIYSGVAGFYDYGPLGGVLKNNIMNLWRKYYVSNEGFYEIEAPTIMPRETLKASGHVDNF
TDPMTQCMECNEVYRADHIIEDAISKEVEGLPDEKLTEIIEENNIVCQNCGAKLTPVKSYNLMFKTEIGVSGKQTGFMRP
ETAQGIFIAFKRISRFYKDKLPFGIVQLGKSYRNELSPRQGVIRLREFTQAEAEIFVDPSDKTHKNYKNIENHVLHLYNQ
KEQLNEGKPIEITVKDAIDNNIVSSEMLIYQIVIAHKFLRDLGIEDDVIRFRQHLPDEMAHYAIDCWDAEVKTDQYGWVE
IIGIADRTDFDLKSHIKHSKEDLSVFKEYDTPKNVTVTKPSFNMKKFGPTFKGDSPKAKEILENTDSNIIKESFDKDGVF
SFSIGENKYEIDQSFVKFETKEEQIRGDRIIPHVIEPSYGIDRIIYSTLLHSYTEDETEEGEKRSYLKLPAKIAPIKAAI
LPLVNKQPLTLIAEDIETTLRSSNIITSFDGSGTIGRRYARADEIGIPYAITIDYDTVDDKKVTIRNRDTFKQKRVEIEK
LSMIINNLVEGHINFEDIEE

Sequences:

>Translated_580_residues
MGNEEVMRISRKRGYLYPSFEIYSGVAGFYDYGPLGGVLKNNIMNLWRKYYVSNEGFYEIEAPTIMPRETLKASGHVDNF
TDPMTQCMECNEVYRADHIIEDAISKEVEGLPDEKLTEIIEENNIVCQNCGAKLTPVKSYNLMFKTEIGVSGKQTGFMRP
ETAQGIFIAFKRISRFYKDKLPFGIVQLGKSYRNELSPRQGVIRLREFTQAEAEIFVDPSDKTHKNYKNIENHVLHLYNQ
KEQLNEGKPIEITVKDAIDNNIVSSEMLIYQIVIAHKFLRDLGIEDDVIRFRQHLPDEMAHYAIDCWDAEVKTDQYGWVE
IIGIADRTDFDLKSHIKHSKEDLSVFKEYDTPKNVTVTKPSFNMKKFGPTFKGDSPKAKEILENTDSNIIKESFDKDGVF
SFSIGENKYEIDQSFVKFETKEEQIRGDRIIPHVIEPSYGIDRIIYSTLLHSYTEDETEEGEKRSYLKLPAKIAPIKAAI
LPLVNKQPLTLIAEDIETTLRSSNIITSFDGSGTIGRRYARADEIGIPYAITIDYDTVDDKKVTIRNRDTFKQKRVEIEK
LSMIINNLVEGHINFEDIEE
>Mature_579_residues
GNEEVMRISRKRGYLYPSFEIYSGVAGFYDYGPLGGVLKNNIMNLWRKYYVSNEGFYEIEAPTIMPRETLKASGHVDNFT
DPMTQCMECNEVYRADHIIEDAISKEVEGLPDEKLTEIIEENNIVCQNCGAKLTPVKSYNLMFKTEIGVSGKQTGFMRPE
TAQGIFIAFKRISRFYKDKLPFGIVQLGKSYRNELSPRQGVIRLREFTQAEAEIFVDPSDKTHKNYKNIENHVLHLYNQK
EQLNEGKPIEITVKDAIDNNIVSSEMLIYQIVIAHKFLRDLGIEDDVIRFRQHLPDEMAHYAIDCWDAEVKTDQYGWVEI
IGIADRTDFDLKSHIKHSKEDLSVFKEYDTPKNVTVTKPSFNMKKFGPTFKGDSPKAKEILENTDSNIIKESFDKDGVFS
FSIGENKYEIDQSFVKFETKEEQIRGDRIIPHVIEPSYGIDRIIYSTLLHSYTEDETEEGEKRSYLKLPAKIAPIKAAIL
PLVNKQPLTLIAEDIETTLRSSNIITSFDGSGTIGRRYARADEIGIPYAITIDYDTVDDKKVTIRNRDTFKQKRVEIEKL
SMIINNLVEGHINFEDIEE

Specific function: Catalyzes the attachment of glycine to tRNA(Gly) [H]

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI116805340, Length=600, Percent_Identity=37.1666666666667, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI32564541, Length=597, Percent_Identity=38.6934673366834, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17554996, Length=597, Percent_Identity=38.6934673366834, Blast_Score=435, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6319597, Length=636, Percent_Identity=36.6352201257862, Blast_Score=418, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6325338, Length=588, Percent_Identity=37.9251700680272, Blast_Score=413, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24664462, Length=597, Percent_Identity=37.85594639866, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI21357965, Length=597, Percent_Identity=37.85594639866, Blast_Score=427, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022960
- InterPro:   IPR002315 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 66705; Mature: 66574

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNEEVMRISRKRGYLYPSFEIYSGVAGFYDYGPLGGVLKNNIMNLWRKYYVSNEGFYEI
CCCHHHHHHHHHCCCCCCCHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
EAPTIMPRETLKASGHVDNFTDPMTQCMECNEVYRADHIIEDAISKEVEGLPDEKLTEII
CCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
EENNIVCQNCGAKLTPVKSYNLMFKTEIGVSGKQTGFMRPETAQGIFIAFKRISRFYKDK
HCCCEEEECCCCEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
LPFGIVQLGKSYRNELSPRQGVIRLREFTQAEAEIFVDPSDKTHKNYKNIENHVLHLYNQ
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCC
KEQLNEGKPIEITVKDAIDNNIVSSEMLIYQIVIAHKFLRDLGIEDDVIRFRQHLPDEMA
HHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHH
HYAIDCWDAEVKTDQYGWVEIIGIADRTDFDLKSHIKHSKEDLSVFKEYDTPKNVTVTKP
HHHHEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHHCCHHHHHHHHHCCCCCCEEEECC
SFNMKKFGPTFKGDSPKAKEILENTDSNIIKESFDKDGVFSFSIGENKYEIDQSFVKFET
CCCHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCC
KEEQIRGDRIIPHVIEPSYGIDRIIYSTLLHSYTEDETEEGEKRSYLKLPAKIAPIKAAI
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCHHHHCCCCHHHHHHHHH
LPLVNKQPLTLIAEDIETTLRSSNIITSFDGSGTIGRRYARADEIGIPYAITIDYDTVDD
HHCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCC
KKVTIRNRDTFKQKRVEIEKLSMIINNLVEGHINFEDIEE
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
GNEEVMRISRKRGYLYPSFEIYSGVAGFYDYGPLGGVLKNNIMNLWRKYYVSNEGFYEI
CCHHHHHHHHHCCCCCCCHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
EAPTIMPRETLKASGHVDNFTDPMTQCMECNEVYRADHIIEDAISKEVEGLPDEKLTEII
CCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
EENNIVCQNCGAKLTPVKSYNLMFKTEIGVSGKQTGFMRPETAQGIFIAFKRISRFYKDK
HCCCEEEECCCCEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
LPFGIVQLGKSYRNELSPRQGVIRLREFTQAEAEIFVDPSDKTHKNYKNIENHVLHLYNQ
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCC
KEQLNEGKPIEITVKDAIDNNIVSSEMLIYQIVIAHKFLRDLGIEDDVIRFRQHLPDEMA
HHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHH
HYAIDCWDAEVKTDQYGWVEIIGIADRTDFDLKSHIKHSKEDLSVFKEYDTPKNVTVTKP
HHHHEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHHCCHHHHHHHHHCCCCCCEEEECC
SFNMKKFGPTFKGDSPKAKEILENTDSNIIKESFDKDGVFSFSIGENKYEIDQSFVKFET
CCCHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCC
KEEQIRGDRIIPHVIEPSYGIDRIIYSTLLHSYTEDETEEGEKRSYLKLPAKIAPIKAAI
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCHHHHCCCCHHHHHHHHH
LPLVNKQPLTLIAEDIETTLRSSNIITSFDGSGTIGRRYARADEIGIPYAITIDYDTVDD
HHCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCC
KKVTIRNRDTFKQKRVEIEKLSMIINNLVEGHINFEDIEE
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]