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The map label for this gene is tfrB

Identifier: 84489839

GI number: 84489839

Start: 1190440

End: 1191936

Strand: Direct

Name: tfrB

Synonym: Msp_1044

Alternate gene names: 84489839

Gene position: 1190440-1191936 (Clockwise)

Preceding gene: 84489836

Following gene: 84489842

Centisome position: 67.36

GC content: 29.26

Gene sequence:

>1497_bases
ATGATTACTGTTAACATAAAAAGATATAATCCAGAAACTAATAAACAATACATGGAATCATATGAAATTGAACATACAGA
TAAAATGAAAGTTTTAGATGCTCTTCAACAAATTAATGATAAATATGATGCTAAAATAGCATATAGATATTCATGTAGAG
CTGGTCAATGTGGTTCATGTGCAATTAAAATAAATGGACAGGCAAAATTAGCATGTAAAGCTGAAATTAATGATAATGAT
ACATTAGAACCATTAGATTTTAAGGTAATTAAGGATCTAATAGTTGACCGTAGTCCATTAAATAAAAAAGTAAATGATTT
AAATCTTTACATGGCATCAGAATCTAATGAAAAACTATTAGAACCTGAAATTATAAAACCTGAAACATATGCTCAAACTG
AAGCATTACGTGGTTGTATTGATTGTTATTCCTGTATATCAATGTGTCCTGTAATTAAAAAATCAACTGAATTTATTGGA
CCTTATTTTATGAGAGCATTTTCTGATTTATCATTTGATCCTAGAGAAGACACATCAAAAAGTGAGGATGCTATTGATTC
TGGTTTATATTCTTGTACTTCTTGTGGACAATGTTCAAAAACATGTCCTAAAGAGATTGATATCTATGGAAAAGGTATTG
AAAAACTTAGGGCAACTGCATTTGCAAGAAAAGAAGGTCCTCTTGAAGCACATAAACAAATACGTGAATCTGTAATAAAT
ACTGGTAGAACTGTTCAACCAATGGAAGATAGTAAATATCCTGAAGGATTCATAAAAGCATATAATCAAACACATACATT
TGAAGAAAAACCTAAAATTGCCTTCTTCACAGGTTGTATGATAGATAATAGATTACCATGGATTGCCGAATATTTAATTA
ATATTCTCTCAAAACTTGGATATGAAGTAGATATCCCTGAACAACAAGTCTGCTGTGGTTCACCACTATTTAGAACTGGA
CAGGTAGATGTTATTCCATCATTAATTAAAAAGAATTATGAAACATTCAAGGATTATGATATAGTATTAACAGTATGTGC
AGGTTGTGGTTCAACACTTAAAAATAATTATCCAGAATATGATGCAAAACTAAATGTAATGGATATAACAGAATTCCTAC
AGGATAAACTTAAAACTGAGGATATGAATAAATTAGATTTAAAAGTAACATATCATGATCCATGTCATCTTGTACGTGGA
CAAGGAATTAGTAAACAACCAAGGAAAATATTAAATAATATTAATGGTGTTGAATTTATTGAAATGGAAAAACCTGACCA
ATGTTGTGGTGCTGGTGGAGGAGTAAAATCTGGAAAACCAGAATTGGCTAAATCTCTAGCAGATAGTAAAGTAGACATGA
TTGATGAGTTAGATGTTGATTATGTTGTAACAATATGTCCATTCTGTGAATTTAATATACAAGATTCATTAACTAATAAA
AATTCAAAAACAGAAGTAATTAACCTTATGGAACTTTTAAATAAGGCATATGAATAA

Upstream 100 bases:

>100_bases
AATTAAAATATAATAAAAAACATATAACTAAAATAGATAGAAAAAATAATTAAATTAATATAATTTTTAATTATTATAAA
GTAATTATTAGGGGTAGTAT

Downstream 100 bases:

>100_bases
TTATATTCATATCTTTCTTTTTTTTAAAAAAATAAATGGTATTTTAATCTAGTAATACTGCTGTACCATTTACACTTACA
AGAATTGTGTTACCCATAGT

Product: succinate dehydrogenase/fumarate reductase iron-sulfur subunit

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MITVNIKRYNPETNKQYMESYEIEHTDKMKVLDALQQINDKYDAKIAYRYSCRAGQCGSCAIKINGQAKLACKAEINDND
TLEPLDFKVIKDLIVDRSPLNKKVNDLNLYMASESNEKLLEPEIIKPETYAQTEALRGCIDCYSCISMCPVIKKSTEFIG
PYFMRAFSDLSFDPREDTSKSEDAIDSGLYSCTSCGQCSKTCPKEIDIYGKGIEKLRATAFARKEGPLEAHKQIRESVIN
TGRTVQPMEDSKYPEGFIKAYNQTHTFEEKPKIAFFTGCMIDNRLPWIAEYLINILSKLGYEVDIPEQQVCCGSPLFRTG
QVDVIPSLIKKNYETFKDYDIVLTVCAGCGSTLKNNYPEYDAKLNVMDITEFLQDKLKTEDMNKLDLKVTYHDPCHLVRG
QGISKQPRKILNNINGVEFIEMEKPDQCCGAGGGVKSGKPELAKSLADSKVDMIDELDVDYVVTICPFCEFNIQDSLTNK
NSKTEVINLMELLNKAYE

Sequences:

>Translated_498_residues
MITVNIKRYNPETNKQYMESYEIEHTDKMKVLDALQQINDKYDAKIAYRYSCRAGQCGSCAIKINGQAKLACKAEINDND
TLEPLDFKVIKDLIVDRSPLNKKVNDLNLYMASESNEKLLEPEIIKPETYAQTEALRGCIDCYSCISMCPVIKKSTEFIG
PYFMRAFSDLSFDPREDTSKSEDAIDSGLYSCTSCGQCSKTCPKEIDIYGKGIEKLRATAFARKEGPLEAHKQIRESVIN
TGRTVQPMEDSKYPEGFIKAYNQTHTFEEKPKIAFFTGCMIDNRLPWIAEYLINILSKLGYEVDIPEQQVCCGSPLFRTG
QVDVIPSLIKKNYETFKDYDIVLTVCAGCGSTLKNNYPEYDAKLNVMDITEFLQDKLKTEDMNKLDLKVTYHDPCHLVRG
QGISKQPRKILNNINGVEFIEMEKPDQCCGAGGGVKSGKPELAKSLADSKVDMIDELDVDYVVTICPFCEFNIQDSLTNK
NSKTEVINLMELLNKAYE
>Mature_498_residues
MITVNIKRYNPETNKQYMESYEIEHTDKMKVLDALQQINDKYDAKIAYRYSCRAGQCGSCAIKINGQAKLACKAEINDND
TLEPLDFKVIKDLIVDRSPLNKKVNDLNLYMASESNEKLLEPEIIKPETYAQTEALRGCIDCYSCISMCPVIKKSTEFIG
PYFMRAFSDLSFDPREDTSKSEDAIDSGLYSCTSCGQCSKTCPKEIDIYGKGIEKLRATAFARKEGPLEAHKQIRESVIN
TGRTVQPMEDSKYPEGFIKAYNQTHTFEEKPKIAFFTGCMIDNRLPWIAEYLINILSKLGYEVDIPEQQVCCGSPLFRTG
QVDVIPSLIKKNYETFKDYDIVLTVCAGCGSTLKNNYPEYDAKLNVMDITEFLQDKLKTEDMNKLDLKVTYHDPCHLVRG
QGISKQPRKILNNINGVEFIEMEKPDQCCGAGGGVKSGKPELAKSLADSKVDMIDELDVDYVVTICPFCEFNIQDSLTNK
NSKTEVINLMELLNKAYE

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI115387094, Length=235, Percent_Identity=29.7872340425532, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1790596, Length=214, Percent_Identity=31.7757009345794, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1788576, Length=388, Percent_Identity=26.0309278350515, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786497, Length=235, Percent_Identity=28.0851063829787, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI48994913, Length=395, Percent_Identity=21.7721518987342, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786943, Length=220, Percent_Identity=28.6363636363636, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17533915, Length=242, Percent_Identity=29.3388429752066, Blast_Score=85, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6322987, Length=233, Percent_Identity=30.9012875536481, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17137106, Length=241, Percent_Identity=29.8755186721992, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24643156, Length=229, Percent_Identity=31.0043668122271, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR004017
- InterPro:   IPR001041
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR004489 [H]

Pfam domain/function: PF02754 CCG; PF00111 Fer2 [H]

EC number: 1.3.99.1

Molecular weight: Translated: 56315; Mature: 56315

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITVNIKRYNPETNKQYMESYEIEHTDKMKVLDALQQINDKYDAKIAYRYSCRAGQCGSC
CEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCE
AIKINGQAKLACKAEINDNDTLEPLDFKVIKDLIVDRSPLNKKVNDLNLYMASESNEKLL
EEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCC
EPEIIKPETYAQTEALRGCIDCYSCISMCPVIKKSTEFIGPYFMRAFSDLSFDPREDTSK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SEDAIDSGLYSCTSCGQCSKTCPKEIDIYGKGIEKLRATAFARKEGPLEAHKQIRESVIN
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TGRTVQPMEDSKYPEGFIKAYNQTHTFEEKPKIAFFTGCMIDNRLPWIAEYLINILSKLG
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCC
YEVDIPEQQVCCGSPLFRTGQVDVIPSLIKKNYETFKDYDIVLTVCAGCGSTLKNNYPEY
CEECCCCHHHHCCCCCCCCCCHHHHHHHHHCCHHHHCCHHHHHHHHHCCCHHHHCCCCCC
DAKLNVMDITEFLQDKLKTEDMNKLDLKVTYHDPCHLVRGQGISKQPRKILNNINGVEFI
CCEEEHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHCCCCCCCCCHHHHHHCCCCCEEE
EMEKPDQCCGAGGGVKSGKPELAKSLADSKVDMIDELDVDYVVTICPFCEFNIQDSLTNK
EECCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCC
NSKTEVINLMELLNKAYE
CCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITVNIKRYNPETNKQYMESYEIEHTDKMKVLDALQQINDKYDAKIAYRYSCRAGQCGSC
CEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCE
AIKINGQAKLACKAEINDNDTLEPLDFKVIKDLIVDRSPLNKKVNDLNLYMASESNEKLL
EEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCC
EPEIIKPETYAQTEALRGCIDCYSCISMCPVIKKSTEFIGPYFMRAFSDLSFDPREDTSK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SEDAIDSGLYSCTSCGQCSKTCPKEIDIYGKGIEKLRATAFARKEGPLEAHKQIRESVIN
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TGRTVQPMEDSKYPEGFIKAYNQTHTFEEKPKIAFFTGCMIDNRLPWIAEYLINILSKLG
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCC
YEVDIPEQQVCCGSPLFRTGQVDVIPSLIKKNYETFKDYDIVLTVCAGCGSTLKNNYPEY
CEECCCCHHHHCCCCCCCCCCHHHHHHHHHCCHHHHCCHHHHHHHHHCCCHHHHCCCCCC
DAKLNVMDITEFLQDKLKTEDMNKLDLKVTYHDPCHLVRGQGISKQPRKILNNINGVEFI
CCEEEHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHCCCCCCCCCHHHHHHCCCCCEEE
EMEKPDQCCGAGGGVKSGKPELAKSLADSKVDMIDELDVDYVVTICPFCEFNIQDSLTNK
EECCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCC
NSKTEVINLMELLNKAYE
CCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.4 {fumarate}} 0.02 {succinate}} 0.3 {ferricyanide}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA