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The map label for this gene is rfbB [H]

Identifier: 84489792

GI number: 84489792

Start: 1142119

End: 1143060

Strand: Direct

Name: rfbB [H]

Synonym: Msp_0993

Alternate gene names: 84489792

Gene position: 1142119-1143060 (Clockwise)

Preceding gene: 84489790

Following gene: 84489797

Centisome position: 64.62

GC content: 31.0

Gene sequence:

>942_bases
ATGGAAACCAATAGAATATTAGTTACTGGTGGTTCAGGCTTTATTGGAACAAATTTAGTAAATGAACTAAGAAGTAGAGG
ACATGAAGTAATAGCAGCAGATTTATTAAATACTGATCGTGAAGATTATGTACGTACAGATGTTAGAAATTATAGACAAA
TTGAACGTACACTTGAAGATGAAGGTCCATTTGATTATGTATATCATCTAGCTGCAGAATATGGACGATGGAATGGAGAA
GCATACTATGAAAATCTATGGCAAACAAATGTTATAGGTACTAAAAATATGATAAGATTACAAGAAAAGAATAAGTTTCG
TATGATTTTCTTTTCATCAGCAGAAGTATATGGAGATTATACTGGAAAAATGTCTGAAGATGTAATGGAAAACAATCCCA
TTAAAGATACCTATCAAATGAATGACTATGCAATAAGTAAATGGGATGGAGAATTAATGTGTATGAACTCAGCAAAAATG
TTTGGTACAGAAACTGTAAGAGTTCGTCCAGTAAATTGTTATGGTCCTGGAGAAAAATATACACCATACAGAGGATTTAT
ACCAAAATTCACATATCTTGCATTACATGATAAACCATACACTGTATATGAAGGACATAAAAGAATCATTGATTATGTTG
GAGATACAGCACGTACCTTTGCAAATATTGTGGATAATTTTATTCCTGGAGAAGCATATAATATTGGTGGAAATCAAGAA
TGGGAAATGGATATTAAAGATTATTCTGACTTAGTATTAGAAGCTACTGGACGTGATGATAGTCTAGTAACATATAAAGA
AGCAGAACCATTCACTACAAAGATAAAAACCATTGATTTTTCAAAATCCATAAGAGATCTTAAACATGATCCTAAAATCA
AACCAAAAGAAGGAATTAAAAAAACAGCTGAATGGATGAAATGGTATTATAGACTTGAATAA

Upstream 100 bases:

>100_bases
TTATTCTCCATATTATAATATTATTCTCTATAATGTATTCTATTAAAATATTAATAGTATCAACTATATATTTTTATACA
ATAATAAATGAAGGGATTTT

Downstream 100 bases:

>100_bases
ATACAAATTATATGAATTATTTCATATATTATACTTTTTTTAAAAAAAAGAATAAATACAATTAAGTATTATATTAAATA
GCATTATATTTATTTTAAAA

Product: putative nucleoside-diphosphate-sugar epimerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
METNRILVTGGSGFIGTNLVNELRSRGHEVIAADLLNTDREDYVRTDVRNYRQIERTLEDEGPFDYVYHLAAEYGRWNGE
AYYENLWQTNVIGTKNMIRLQEKNKFRMIFFSSAEVYGDYTGKMSEDVMENNPIKDTYQMNDYAISKWDGELMCMNSAKM
FGTETVRVRPVNCYGPGEKYTPYRGFIPKFTYLALHDKPYTVYEGHKRIIDYVGDTARTFANIVDNFIPGEAYNIGGNQE
WEMDIKDYSDLVLEATGRDDSLVTYKEAEPFTTKIKTIDFSKSIRDLKHDPKIKPKEGIKKTAEWMKWYYRLE

Sequences:

>Translated_313_residues
METNRILVTGGSGFIGTNLVNELRSRGHEVIAADLLNTDREDYVRTDVRNYRQIERTLEDEGPFDYVYHLAAEYGRWNGE
AYYENLWQTNVIGTKNMIRLQEKNKFRMIFFSSAEVYGDYTGKMSEDVMENNPIKDTYQMNDYAISKWDGELMCMNSAKM
FGTETVRVRPVNCYGPGEKYTPYRGFIPKFTYLALHDKPYTVYEGHKRIIDYVGDTARTFANIVDNFIPGEAYNIGGNQE
WEMDIKDYSDLVLEATGRDDSLVTYKEAEPFTTKIKTIDFSKSIRDLKHDPKIKPKEGIKKTAEWMKWYYRLE
>Mature_313_residues
METNRILVTGGSGFIGTNLVNELRSRGHEVIAADLLNTDREDYVRTDVRNYRQIERTLEDEGPFDYVYHLAAEYGRWNGE
AYYENLWQTNVIGTKNMIRLQEKNKFRMIFFSSAEVYGDYTGKMSEDVMENNPIKDTYQMNDYAISKWDGELMCMNSAKM
FGTETVRVRPVNCYGPGEKYTPYRGFIPKFTYLALHDKPYTVYEGHKRIIDYVGDTARTFANIVDNFIPGEAYNIGGNQE
WEMDIKDYSDLVLEATGRDDSLVTYKEAEPFTTKIKTIDFSKSIRDLKHDPKIKPKEGIKKTAEWMKWYYRLE

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=313, Percent_Identity=28.4345047923323, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=27.6595744680851, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1788353, Length=336, Percent_Identity=28.2738095238095, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI48994969, Length=339, Percent_Identity=26.8436578171091, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=25.6797583081571, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=339, Percent_Identity=28.3185840707965, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356223, Length=323, Percent_Identity=25.3869969040248, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36563; Mature: 36563

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METNRILVTGGSGFIGTNLVNELRSRGHEVIAADLLNTDREDYVRTDVRNYRQIERTLED
CCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHC
EGPFDYVYHLAAEYGRWNGEAYYENLWQTNVIGTKNMIRLQEKNKFRMIFFSSAEVYGDY
CCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCEEECCC
TGKMSEDVMENNPIKDTYQMNDYAISKWDGELMCMNSAKMFGTETVRVRPVNCYGPGEKY
CCCCHHHHHCCCCCCCEEECCCEEEEECCCCEEEECCCHHCCCCEEEEEEEEECCCCCCC
TPYRGFIPKFTYLALHDKPYTVYEGHKRIIDYVGDTARTFANIVDNFIPGEAYNIGGNQE
CCCCCCCCCEEEEEEECCCCEEECCHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCCCCE
WEMDIKDYSDLVLEATGRDDSLVTYKEAEPFTTKIKTIDFSKSIRDLKHDPKIKPKEGIK
EEECHHCCCCEEEEECCCCCCEEEEECCCCCEEEEEEEEHHHHHHHHHCCCCCCCHHHHH
KTAEWMKWYYRLE
HHHHHHHHHHCCC
>Mature Secondary Structure
METNRILVTGGSGFIGTNLVNELRSRGHEVIAADLLNTDREDYVRTDVRNYRQIERTLED
CCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHC
EGPFDYVYHLAAEYGRWNGEAYYENLWQTNVIGTKNMIRLQEKNKFRMIFFSSAEVYGDY
CCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCEEECCC
TGKMSEDVMENNPIKDTYQMNDYAISKWDGELMCMNSAKMFGTETVRVRPVNCYGPGEKY
CCCCHHHHHCCCCCCCEEECCCEEEEECCCCEEEECCCHHCCCCEEEEEEEEECCCCCCC
TPYRGFIPKFTYLALHDKPYTVYEGHKRIIDYVGDTARTFANIVDNFIPGEAYNIGGNQE
CCCCCCCCCEEEEEEECCCCEEECCHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCCCCE
WEMDIKDYSDLVLEATGRDDSLVTYKEAEPFTTKIKTIDFSKSIRDLKHDPKIKPKEGIK
EEECHHCCCCEEEEECCCCCCEEEEECCCCCEEEEEEEEHHHHHHHHHCCCCCCCHHHHH
KTAEWMKWYYRLE
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]