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The map label for this gene is thiM1

Identifier: 84489491

GI number: 84489491

Start: 795530

End: 796387

Strand: Direct

Name: thiM1

Synonym: Msp_0682

Alternate gene names: 84489491

Gene position: 795530-796387 (Clockwise)

Preceding gene: 84489490

Following gene: 84489492

Centisome position: 45.01

GC content: 29.02

Gene sequence:

>858_bases
ATGAGTGAATATACATTAGAAAATATAGCACAAGTTGTAGAAATATTAAGAGAAAAAAGTCCATTAACACATTGTATTAC
AAATGTAGTTACTGTTAAAGATTGTGCTAATGCTGTTTTAGCAGTAGGAGCATCACCAATAATGGCAAATGCTCCAGAAG
AAGCAGAAGAAATTGTAGGTATTTCTAATTCACTTGTAATAAATATTGGAACATTAACTAAAGAACAAATAGAAACAATG
AAGAAATCAGCAAAATTTGCTATTGAAAATAATAAGCCATTCATACTAGATCCTGTAGGTGTAGGTATTAGTAACATTAG
AAATCAAACACCAATCGATATTATTACAAATTCAAAACCATCTATTATTAGAGGAAATCTTTCAGAAATCAAAGCAATAG
CAATGATGTATGATATATTAGATGAATGTACAATGGCAAAAGGTGTTGATGTAGCTCAATGTGATATTATAAACAAAGAT
ACATTAATTTCTAATTGTAATCTTATTAAGAATATTTCAGAAAAATTAAATACTACAATAGCAGTATCTGGACCAATTGA
TATAATATCTGATGGACATGATGTATACACAATAGAAAATGGTGATGCTATGATGTCTAGAATAACAGGTAGTGGATGTA
TGTTAGGATGTGTACTTGGAGCATATCTTGCAGTAACAAATCCATTAGAAGCAGCAATTACAGGTACACTTGTAATGGGT
ATAGCTGGAGAATTAGCTGCAAAAACAGCAAGAGATAATAACAAAGGAACAGGTTCATTTGGAATATATCTTATAGATGA
ATTATCAAAATTAAATAAAAGCACAATACTTTCTCAATCAAAATTAAATAAAATGTAG

Upstream 100 bases:

>100_bases
TTTTTAGACTTTTTTTATTTTATTTTTTTGAATTAGACATTATATTAATCATTAGAATCAAAAATATAATTAGTAATAAA
AATTTTTGGAGAATTAAATT

Downstream 100 bases:

>100_bases
GTAATTGAAAATGGATATTGATTATAGTGTCTATTTAGTAACTGATCAATTTGATTTTACTGAATCAGAATTTTTAAATA
TTATAGAGGAAGCTCTAAAA

Product: ThiM1

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase 1; TH kinase 1; Thz kinase 1

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSEYTLENIAQVVEILREKSPLTHCITNVVTVKDCANAVLAVGASPIMANAPEEAEEIVGISNSLVINIGTLTKEQIETM
KKSAKFAIENNKPFILDPVGVGISNIRNQTPIDIITNSKPSIIRGNLSEIKAIAMMYDILDECTMAKGVDVAQCDIINKD
TLISNCNLIKNISEKLNTTIAVSGPIDIISDGHDVYTIENGDAMMSRITGSGCMLGCVLGAYLAVTNPLEAAITGTLVMG
IAGELAAKTARDNNKGTGSFGIYLIDELSKLNKSTILSQSKLNKM

Sequences:

>Translated_285_residues
MSEYTLENIAQVVEILREKSPLTHCITNVVTVKDCANAVLAVGASPIMANAPEEAEEIVGISNSLVINIGTLTKEQIETM
KKSAKFAIENNKPFILDPVGVGISNIRNQTPIDIITNSKPSIIRGNLSEIKAIAMMYDILDECTMAKGVDVAQCDIINKD
TLISNCNLIKNISEKLNTTIAVSGPIDIISDGHDVYTIENGDAMMSRITGSGCMLGCVLGAYLAVTNPLEAAITGTLVMG
IAGELAAKTARDNNKGTGSFGIYLIDELSKLNKSTILSQSKLNKM
>Mature_284_residues
SEYTLENIAQVVEILREKSPLTHCITNVVTVKDCANAVLAVGASPIMANAPEEAEEIVGISNSLVINIGTLTKEQIETMK
KSAKFAIENNKPFILDPVGVGISNIRNQTPIDIITNSKPSIIRGNLSEIKAIAMMYDILDECTMAKGVDVAQCDIINKDT
LISNCNLIKNISEKLNTTIAVSGPIDIISDGHDVYTIENGDAMMSRITGSGCMLGCVLGAYLAVTNPLEAAITGTLVMGI
AGELAAKTARDNNKGTGSFGIYLIDELSKLNKSTILSQSKLNKM

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=265, Percent_Identity=32.8301886792453, Blast_Score=145, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6325042, Length=291, Percent_Identity=29.8969072164948, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM1_METST (Q2NGH3)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447723.1
- ProteinModelPortal:   Q2NGH3
- SMR:   Q2NGH3
- STRING:   Q2NGH3
- GeneID:   3855870
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0682
- NMPDR:   fig|339860.6.peg.662
- eggNOG:   arNOG07431
- HOGENOM:   HBG351126
- PhylomeDB:   Q2NGH3
- ProtClustDB:   CLSK843662
- BioCyc:   MSTA339860:MSP_0682-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 30379; Mature: 30247

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: BINDING 48-48 BINDING 124-124 BINDING 183-183 BINDING 210-210

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYTLENIAQVVEILREKSPLTHCITNVVTVKDCANAVLAVGASPIMANAPEEAEEIVG
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHC
ISNSLVINIGTLTKEQIETMKKSAKFAIENNKPFILDPVGVGISNIRNQTPIDIITNSKP
CCCCEEEEECCCCHHHHHHHHHHCCEEEECCCCEEECCCCCCHHHHCCCCCEEEEECCCC
SIIRGNLSEIKAIAMMYDILDECTMAKGVDVAQCDIINKDTLISNCNLIKNISEKLNTTI
CEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHCCHHHHHHHHHHCCEE
AVSGPIDIISDGHDVYTIENGDAMMSRITGSGCMLGCVLGAYLAVTNPLEAAITGTLVMG
EECCCEEEECCCCEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IAGELAAKTARDNNKGTGSFGIYLIDELSKLNKSTILSQSKLNKM
HHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEYTLENIAQVVEILREKSPLTHCITNVVTVKDCANAVLAVGASPIMANAPEEAEEIVG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHC
ISNSLVINIGTLTKEQIETMKKSAKFAIENNKPFILDPVGVGISNIRNQTPIDIITNSKP
CCCCEEEEECCCCHHHHHHHHHHCCEEEECCCCEEECCCCCCHHHHCCCCCEEEEECCCC
SIIRGNLSEIKAIAMMYDILDECTMAKGVDVAQCDIINKDTLISNCNLIKNISEKLNTTI
CEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHCCHHHHHHHHHHCCEE
AVSGPIDIISDGHDVYTIENGDAMMSRITGSGCMLGCVLGAYLAVTNPLEAAITGTLVMG
EECCCEEEECCCCEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IAGELAAKTARDNNKGTGSFGIYLIDELSKLNKSTILSQSKLNKM
HHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA