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The map label for this gene is mjaIIIM [H]

Identifier: 84488972

GI number: 84488972

Start: 167844

End: 168782

Strand: Direct

Name: mjaIIIM [H]

Synonym: Msp_0143

Alternate gene names: 84488972

Gene position: 167844-168782 (Clockwise)

Preceding gene: 84488969

Following gene: 84488973

Centisome position: 9.5

GC content: 23.32

Gene sequence:

>939_bases
ATGCAAGTAACTTTATTTAATGACAATATTGTAGAAGGAAAGCCTTTTATGAAATGGCTTGGTGGAAAAAGACAATTAAT
TCCTGAGATAGAAAAAAGATTACCAGAACATATTAAGATATCAAAGAAAATAGATTCATATTTTGAACCCTTTATTGGTG
GAGGAGCTTTATTTTTTTATCTTATGAGTAATTATCATATTAAAAAAGCATATATTTCTGATATTAATAAAGAATTGATA
TTAATATATAATGCTATAAAAAATGATGATAAAAAAGTAATTACTTATTTAAAAGAAATGGAAAGTGAATTTTTAAAATT
AGATAATGATAAACGTAAAGCATATTATTTAAATGTTAGAGAAGAATTTAATAATCAATTAAAAACATATGATTTTAAAA
ATTATTCTGAAGAAACAATAAAAAGAGCTGTTAAATCAATGTTCATGAATAAAACAGGATTTAATGGATTATTTAGATTA
AATAAGAAAGGTGAATTTAACGTGCCTTTTGGAAGATATAAAAATCCTAAAATATGTGATGAAACCAATATTCATAATGT
TCATAAAATTCTAAAAAATGTTACTATAAAAAATAAACCCTATGATTATTCTGAAAAATACATTGAAAAAGGTTCACTAG
TATACTTAGATCCACCATACAGACCAATTTCTAAAACTTCCAGTTTCACAAGCTATTCTGATAATGAATTTAATGATGAA
CATCAAATAGAATTAGCACATTATTATGAGAGAATATCCAATAAAGGAGCATATGCTTTGTTAAGTAATAGTGATCCTCA
TAATAATGATCCAAATGATGATTTTTTTGATTTATTATATTCTAAATATTGTATTGATAGAATTCCAGCAAAAAGAAGTA
TTAATTCAAAAGGAAACAAACGTGGACCAGTAAATGAATTGTTGATAAAAAATTATTGA

Upstream 100 bases:

>100_bases
ATTTATGATTCTATTTTTCAATATCAGTTCTGAAGATCTATTTAATAGAAATGGATATTAACTAAAACTATATATTAGTA
GGAATAGAAGAGTATGTATT

Downstream 100 bases:

>100_bases
GGTTAAACTATGGTTAAATATGAAGTATTAGGTTACACATCACAAGAGGAATATAAGGAAGATTTCTTCAATAATTTATT
ACCTACAAATCATAGTTTTA

Product: putative type II restriction-modification system, adenine-specific DNA methyltransferase

Products: NA

Alternate protein names: M.MjaIII; Adenine-specific methyltransferase MjaIII [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MQVTLFNDNIVEGKPFMKWLGGKRQLIPEIEKRLPEHIKISKKIDSYFEPFIGGGALFFYLMSNYHIKKAYISDINKELI
LIYNAIKNDDKKVITYLKEMESEFLKLDNDKRKAYYLNVREEFNNQLKTYDFKNYSEETIKRAVKSMFMNKTGFNGLFRL
NKKGEFNVPFGRYKNPKICDETNIHNVHKILKNVTIKNKPYDYSEKYIEKGSLVYLDPPYRPISKTSSFTSYSDNEFNDE
HQIELAHYYERISNKGAYALLSNSDPHNNDPNDDFFDLLYSKYCIDRIPAKRSINSKGNKRGPVNELLIKNY

Sequences:

>Translated_312_residues
MQVTLFNDNIVEGKPFMKWLGGKRQLIPEIEKRLPEHIKISKKIDSYFEPFIGGGALFFYLMSNYHIKKAYISDINKELI
LIYNAIKNDDKKVITYLKEMESEFLKLDNDKRKAYYLNVREEFNNQLKTYDFKNYSEETIKRAVKSMFMNKTGFNGLFRL
NKKGEFNVPFGRYKNPKICDETNIHNVHKILKNVTIKNKPYDYSEKYIEKGSLVYLDPPYRPISKTSSFTSYSDNEFNDE
HQIELAHYYERISNKGAYALLSNSDPHNNDPNDDFFDLLYSKYCIDRIPAKRSINSKGNKRGPVNELLIKNY
>Mature_312_residues
MQVTLFNDNIVEGKPFMKWLGGKRQLIPEIEKRLPEHIKISKKIDSYFEPFIGGGALFFYLMSNYHIKKAYISDINKELI
LIYNAIKNDDKKVITYLKEMESEFLKLDNDKRKAYYLNVREEFNNQLKTYDFKNYSEETIKRAVKSMFMNKTGFNGLFRL
NKKGEFNVPFGRYKNPKICDETNIHNVHKILKNVTIKNKPYDYSEKYIEKGSLVYLDPPYRPISKTSSFTSYSDNEFNDE
HQIELAHYYERISNKGAYALLSNSDPHNNDPNDDFFDLLYSKYCIDRIPAKRSINSKGNKRGPVNELLIKNY

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MjaIII endonuclease (Potential) [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=295, Percent_Identity=32.8813559322034, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 36835; Mature: 36835

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVTLFNDNIVEGKPFMKWLGGKRQLIPEIEKRLPEHIKISKKIDSYFEPFIGGGALFFY
CEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHH
LMSNYHIKKAYISDINKELILIYNAIKNDDKKVITYLKEMESEFLKLDNDKRKAYYLNVR
HHHCCCCHHHHHHHCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEH
EEFNNQLKTYDFKNYSEETIKRAVKSMFMNKTGFNGLFRLNKKGEFNVPFGRYKNPKICD
HHHCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCC
ETNIHNVHKILKNVTIKNKPYDYSEKYIEKGSLVYLDPPYRPISKTSSFTSYSDNEFNDE
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
HQIELAHYYERISNKGAYALLSNSDPHNNDPNDDFFDLLYSKYCIDRIPAKRSINSKGNK
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RGPVNELLIKNY
CCCHHHHHHCCC
>Mature Secondary Structure
MQVTLFNDNIVEGKPFMKWLGGKRQLIPEIEKRLPEHIKISKKIDSYFEPFIGGGALFFY
CEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHH
LMSNYHIKKAYISDINKELILIYNAIKNDDKKVITYLKEMESEFLKLDNDKRKAYYLNVR
HHHCCCCHHHHHHHCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEH
EEFNNQLKTYDFKNYSEETIKRAVKSMFMNKTGFNGLFRLNKKGEFNVPFGRYKNPKICD
HHHCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCC
ETNIHNVHKILKNVTIKNKPYDYSEKYIEKGSLVYLDPPYRPISKTSSFTSYSDNEFNDE
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
HQIELAHYYERISNKGAYALLSNSDPHNNDPNDDFFDLLYSKYCIDRIPAKRSINSKGNK
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RGPVNELLIKNY
CCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]