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The map label for this gene is polC [H]

Identifier: 84488969

GI number: 84488969

Start: 157926

End: 159263

Strand: Direct

Name: polC [H]

Synonym: Msp_0140

Alternate gene names: 84488969

Gene position: 157926-159263 (Clockwise)

Preceding gene: 84488968

Following gene: 84488972

Centisome position: 8.94

GC content: 26.38

Gene sequence:

>1338_bases
ATGAGAGACTATGTTGTAATGGATCTTGAAAATCCAAATTTTAGACAAAATTCAATATGTGCAATAGGTGTTATGTTAAT
TAGAAATAATAATGTTGTAGAAAGAAAATATTCTCTTATAAATCCTGAAGATACATTTGATAATATTAATATTCAAATAA
CAAAAATAGCACCACACATGATAAAACAAAGTCCAACACTCCCTGAATATTGGAGTGAAATAAGCTCTTGGTTATCAAAT
AATGTGATTGTTGGACATAATATAACATATGATCTTAGAGTACTGACTAAATCACTTCAAAGATATGATTTAGAAGTTCC
AGAATTTAATTACTGCTGTACTCTAACACAAAGTAGAAAAAATTTGGATTTGCCATCATATAAACTAGAAAATATAGCTA
AAAAGTTACATATAATATATAATCCACACAATGCAATAGAAGATGCAAGAGCAGCATATGAATTATTTGAATACATAAAT
AGACATAATCCAATAGGAACTAATCAAGTAAAGCAGTATAAATACAAGCCAAAGACAGAATCATATGATCCTAAATTATC
AACTAACATAAATAATCTTTATGGAATGGTACAAGTTTTAATATATAATCAATCATCAACACAAAAACAATTAAATCTAC
TAAATTCATGGCTACAAGAAAATATGAAATATAATCACTATCCCTTATTTGATGATATTACAAAAAAAATAACATCTATC
GTTGATAAAGGATGTGTTAATGGGGAAGATAAGGAAAAACTCGCTACTATTGAAAGTGTTAATCAATCCAATATATATAA
ACCAAATACACTTAAAACACAAGTATTACAGGGAATTATAAAAATTATCACAGCTGATAATAAAATAACACATGAAGAAC
TTAAATACTTAGATTCATGGTTAGATCAAAATAAATCCCTTAAAGGAACATACCCTTATGATAAAATAGTAGAAATAACA
ACGTCTCTTCTAAAAAAGAATACTGTTGGAGAAAATGAATATATAAACGTTTCAAAGATGTTTTTAGAGCTTTTAAGTCC
AATTAAAACAACAGTTGAGTCTCTTGATTTAGAGGGAAAAACATATTGTTTAACAGGTGATTTTAAACATGGAAATAAGG
CTAAAATAGTATCAATTCTTGAAAAAAGAGGTCTTATTAAGAAAAATTGTGTATCATACAAGTTGGATTATTTGTTTGTT
GGAGATTATGGAAGTCCTGCATGGAAGTATGGTAATATTGGTGGAAAAATTGTTAAAGCACAACAAATAATAGATAAAGG
TGCTAAAATTAAAATTATTAGTGAAAAAAATTTATTTAATGAATTGGGTATTGAATAA

Upstream 100 bases:

>100_bases
ACAACTAGATGAAAAATAGATTTTTAGTCATTGTTTTTTTACTTTTTTTTAAAACTTTTTTTTAGGAAAACTTATTTTAT
TTATTAATATAGTGATTGTT

Downstream 100 bases:

>100_bases
TGTTTTAACTCTTTTATTATTATATGGGATTTGTAGAAACGCTATGTTCATAATATAATTTTTAAAAGTAGTATAATGGT
AAAATTGTGCATGCTTCAAC

Product: 3'-5' exonuclease

Products: NA

Alternate protein names: PolIII [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MRDYVVMDLENPNFRQNSICAIGVMLIRNNNVVERKYSLINPEDTFDNINIQITKIAPHMIKQSPTLPEYWSEISSWLSN
NVIVGHNITYDLRVLTKSLQRYDLEVPEFNYCCTLTQSRKNLDLPSYKLENIAKKLHIIYNPHNAIEDARAAYELFEYIN
RHNPIGTNQVKQYKYKPKTESYDPKLSTNINNLYGMVQVLIYNQSSTQKQLNLLNSWLQENMKYNHYPLFDDITKKITSI
VDKGCVNGEDKEKLATIESVNQSNIYKPNTLKTQVLQGIIKIITADNKITHEELKYLDSWLDQNKSLKGTYPYDKIVEIT
TSLLKKNTVGENEYINVSKMFLELLSPIKTTVESLDLEGKTYCLTGDFKHGNKAKIVSILEKRGLIKKNCVSYKLDYLFV
GDYGSPAWKYGNIGGKIVKAQQIIDKGAKIKIISEKNLFNELGIE

Sequences:

>Translated_445_residues
MRDYVVMDLENPNFRQNSICAIGVMLIRNNNVVERKYSLINPEDTFDNINIQITKIAPHMIKQSPTLPEYWSEISSWLSN
NVIVGHNITYDLRVLTKSLQRYDLEVPEFNYCCTLTQSRKNLDLPSYKLENIAKKLHIIYNPHNAIEDARAAYELFEYIN
RHNPIGTNQVKQYKYKPKTESYDPKLSTNINNLYGMVQVLIYNQSSTQKQLNLLNSWLQENMKYNHYPLFDDITKKITSI
VDKGCVNGEDKEKLATIESVNQSNIYKPNTLKTQVLQGIIKIITADNKITHEELKYLDSWLDQNKSLKGTYPYDKIVEIT
TSLLKKNTVGENEYINVSKMFLELLSPIKTTVESLDLEGKTYCLTGDFKHGNKAKIVSILEKRGLIKKNCVSYKLDYLFV
GDYGSPAWKYGNIGGKIVKAQQIIDKGAKIKIISEKNLFNELGIE
>Mature_445_residues
MRDYVVMDLENPNFRQNSICAIGVMLIRNNNVVERKYSLINPEDTFDNINIQITKIAPHMIKQSPTLPEYWSEISSWLSN
NVIVGHNITYDLRVLTKSLQRYDLEVPEFNYCCTLTQSRKNLDLPSYKLENIAKKLHIIYNPHNAIEDARAAYELFEYIN
RHNPIGTNQVKQYKYKPKTESYDPKLSTNINNLYGMVQVLIYNQSSTQKQLNLLNSWLQENMKYNHYPLFDDITKKITSI
VDKGCVNGEDKEKLATIESVNQSNIYKPNTLKTQVLQGIIKIITADNKITHEELKYLDSWLDQNKSLKGTYPYDKIVEIT
TSLLKKNTVGENEYINVSKMFLELLSPIKTTVESLDLEGKTYCLTGDFKHGNKAKIVSILEKRGLIKKNCVSYKLDYLFV
GDYGSPAWKYGNIGGKIVKAQQIIDKGAKIKIISEKNLFNELGIE

Specific function: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR006054
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR016195
- InterPro:   IPR006308
- InterPro:   IPR012337 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF00929 Exonuc_X-T; PF02811 PHP; PF01336 tRNA_anti [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 51283; Mature: 51283

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDYVVMDLENPNFRQNSICAIGVMLIRNNNVVERKYSLINPEDTFDNINIQITKIAPHM
CCCEEEEECCCCCCCCCCEEEEEEEEEECCCEEHHHHCCCCCCCCCCCCCEEEEECCHHH
IKQSPTLPEYWSEISSWLSNNVIVGHNITYDLRVLTKSLQRYDLEVPEFNYCCTLTQSRK
HHCCCCCHHHHHHHHHHHHCCEEEECCCEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
NLDLPSYKLENIAKKLHIIYNPHNAIEDARAAYELFEYINRHNPIGTNQVKQYKYKPKTE
CCCCCCHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCC
SYDPKLSTNINNLYGMVQVLIYNQSSTQKQLNLLNSWLQENMKYNHYPLFDDITKKITSI
CCCCCCCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
VDKGCVNGEDKEKLATIESVNQSNIYKPNTLKTQVLQGIIKIITADNKITHEELKYLDSW
HHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LDQNKSLKGTYPYDKIVEITTSLLKKNTVGENEYINVSKMFLELLSPIKTTVESLDLEGK
HCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCC
TYCLTGDFKHGNKAKIVSILEKRGLIKKNCVSYKLDYLFVGDYGSPAWKYGNIGGKIVKA
EEEEECCCCCCCHHHHHHHHHHCCCHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCEEHH
QQIIDKGAKIKIISEKNLFNELGIE
HHHHCCCCEEEEEECCCHHHHHCCC
>Mature Secondary Structure
MRDYVVMDLENPNFRQNSICAIGVMLIRNNNVVERKYSLINPEDTFDNINIQITKIAPHM
CCCEEEEECCCCCCCCCCEEEEEEEEEECCCEEHHHHCCCCCCCCCCCCCEEEEECCHHH
IKQSPTLPEYWSEISSWLSNNVIVGHNITYDLRVLTKSLQRYDLEVPEFNYCCTLTQSRK
HHCCCCCHHHHHHHHHHHHCCEEEECCCEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
NLDLPSYKLENIAKKLHIIYNPHNAIEDARAAYELFEYINRHNPIGTNQVKQYKYKPKTE
CCCCCCHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCC
SYDPKLSTNINNLYGMVQVLIYNQSSTQKQLNLLNSWLQENMKYNHYPLFDDITKKITSI
CCCCCCCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
VDKGCVNGEDKEKLATIESVNQSNIYKPNTLKTQVLQGIIKIITADNKITHEELKYLDSW
HHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LDQNKSLKGTYPYDKIVEITTSLLKKNTVGENEYINVSKMFLELLSPIKTTVESLDLEGK
HCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCC
TYCLTGDFKHGNKAKIVSILEKRGLIKKNCVSYKLDYLFVGDYGSPAWKYGNIGGKIVKA
EEEEECCCCCCCHHHHHHHHHHCCCHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCEEHH
QQIIDKGAKIKIISEKNLFNELGIE
HHHHCCCCEEEEEECCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]