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The map label for this gene is emrB [H]

Identifier: 83721667

GI number: 83721667

Start: 23301

End: 24863

Strand: Direct

Name: emrB [H]

Synonym: BTH_I0022

Alternate gene names: 83721667

Gene position: 23301-24863 (Clockwise)

Preceding gene: 83718884

Following gene: 83719078

Centisome position: 0.61

GC content: 66.73

Gene sequence:

>1563_bases
ATGGCTGCGACGGCCCCCGCTTCCGCTTCCCTTTCCGCCGAGCCGGCGCCGCTGTCGGGCGGCACGCTCGCGCTGCTGAC
GGTCGGGCTCGCGCTCGGCACGTTCATGGAGGTGCTCGACACGTCGATCGCGAACGTCGCGGTGCCAACGATCTCCGGCA
GCCTCGGCGTCGCGACGAGCGAAGGCACGTGGGTGATCTCGTCGTACTCCGTCGCCTCCGCGATCGCGGTGCCGCTCACC
GGCTGGCTCGCGCGGCGGGTGGGCGAGGTGCGGCTCTTCACGCTGTCGGTGCTCGCGTTTACGATCGCGTCGGCGCTTTG
CGGCCTCGCTGAGAATTTCGAATCGCTGATCGCGTTCCGGCTGCTGCAGGGCCTCGTGTCCGGCCCGATGGTGCCGCTGT
CGCAGACGATCCTGATGCGCAGCTATCCGCCCGCGAAGCGCGGGCTCGCACTCGGCCTGTGGGCGATGACGGTGATCGTC
GCGCCGATCTTCGGCCCCGTGATGGGCGGCTGGATCACCGACAACTACACGTGGCCGTGGATCTTCTATATCAACCTGCC
GATCGGCATGTTCTCGGCCGCGTGCGCGTTCTTCCTGCTGCGCGGCCGCGAGACGAAGACGACGAAGCAGCGGATCGACG
CGATCGGGCTCGCGCTCCTCGTGATCGGCGTGTCGTGCCTGCAGATGATGCTCGATCTCGGCAAGGACCGTGACTGGTTC
AATTCGACGTTCATCACGTCGCTCGCGCTGATCGCCGTCGTGTCGCTCGCGTTCATGCTCGTGTGGGAGGCGACCGAGAA
GGAGCCGGTCGTCGATCTGTCGCTCTTCAAGGACCGCAACTTCGCGCTCGGCGCGATGATCATCTCGTTCGGCTTCATGG
CGTTCTTCGGCTCGGTCGTGATCTTCCCGCTGTGGCTGCAGACCGTGATGGGCTACACGGCGGGCCTGGCCGGCCTCGCG
ACCGCGCCCGTCGGCTTCCTCGCGCTCGTGCTGTCGCCGCTCATCGGCCGCAACATGCACCGGCTCGATCTGCGGATGGT
CGCGAGCTTCGCGTTCGTCGTGTTCGCCGGCGTGTCGATCTGGAACTCGACCTTCACGCTCGACGTGCCGTTCAACCACG
TGATCCTGCCGCGGCTCGTGCAGGGGATCGGCGTCGCGTGCTTCTTCGTGCCGATGACGACGATCACGCTCTCCAGCATC
TCCGACGAGCGGCTCGCGAGTGCGTCGGGGCTGTCGAACTTCCTGCGCACGCTGTCGGGCGCGATCGGCACCGCGGTCAG
CTCGACGTTCTGGGAAAACGATGCGATCTATCACCACGCGCGGCTCGCCGAATCCGTGGACGTCTACGCGCAGAGCACGA
TCGACTTTCAAGGCGCGCTCGCGCGGCTCGGCATCGTGGGCGACGTGTCGACCGCGCAGATCAACCAGATCGTCACGCAG
CAGGGCTTCATGATGGCGACCAACGACTTCTTTCACATTTCGGCGCTCGCGTTCGTCGCGCTCGCGGCGCTCGTGTGGGT
GACGAAGCCGAAGAAGGGAGCCGGGCCCGCGATGGGGCATTGA

Upstream 100 bases:

>100_bases
TATTTATCTGTCTGGGCAGAAGCTGACAAGACACTTACTGTCGGTCGATGTGGATGCACAAAAGGATGACGGGGCAACAT
TCGCCCCTTAGGAGAGACGC

Downstream 100 bases:

>100_bases
GCGGCGGGCTCCCGCGCGCGCCGTGCGTCATGCGCCGCGCGCCTCGGGCGGTTCGTGTGCGGCAGGATGAGGGCAGGGCG
CGCCCGTGCGACGCCGCGCG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MAATAPASASLSAEPAPLSGGTLALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLT
GWLARRVGEVRLFTLSVLAFTIASALCGLAENFESLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIV
APIFGPVMGGWITDNYTWPWIFYINLPIGMFSAACAFFLLRGRETKTTKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
NSTFITSLALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVVIFPLWLQTVMGYTAGLAGLA
TAPVGFLALVLSPLIGRNMHRLDLRMVASFAFVVFAGVSIWNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSI
SDERLASASGLSNFLRTLSGAIGTAVSSTFWENDAIYHHARLAESVDVYAQSTIDFQGALARLGIVGDVSTAQINQIVTQ
QGFMMATNDFFHISALAFVALAALVWVTKPKKGAGPAMGH

Sequences:

>Translated_520_residues
MAATAPASASLSAEPAPLSGGTLALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLT
GWLARRVGEVRLFTLSVLAFTIASALCGLAENFESLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIV
APIFGPVMGGWITDNYTWPWIFYINLPIGMFSAACAFFLLRGRETKTTKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
NSTFITSLALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVVIFPLWLQTVMGYTAGLAGLA
TAPVGFLALVLSPLIGRNMHRLDLRMVASFAFVVFAGVSIWNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSI
SDERLASASGLSNFLRTLSGAIGTAVSSTFWENDAIYHHARLAESVDVYAQSTIDFQGALARLGIVGDVSTAQINQIVTQ
QGFMMATNDFFHISALAFVALAALVWVTKPKKGAGPAMGH
>Mature_519_residues
AATAPASASLSAEPAPLSGGTLALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLTG
WLARRVGEVRLFTLSVLAFTIASALCGLAENFESLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVA
PIFGPVMGGWITDNYTWPWIFYINLPIGMFSAACAFFLLRGRETKTTKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWFN
STFITSLALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVVIFPLWLQTVMGYTAGLAGLAT
APVGFLALVLSPLIGRNMHRLDLRMVASFAFVVFAGVSIWNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSIS
DERLASASGLSNFLRTLSGAIGTAVSSTFWENDAIYHHARLAESVDVYAQSTIDFQGALARLGIVGDVSTAQINQIVTQQ
GFMMATNDFFHISALAFVALAALVWVTKPKKGAGPAMGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=498, Percent_Identity=51.8072289156626, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1788710, Length=515, Percent_Identity=46.9902912621359, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI1790195, Length=414, Percent_Identity=31.8840579710145, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1788392, Length=411, Percent_Identity=29.683698296837, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI87081983, Length=425, Percent_Identity=24.9411764705882, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6322958, Length=213, Percent_Identity=31.924882629108, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=129, Percent_Identity=29.4573643410853, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=28, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55395; Mature: 55263

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATAPASASLSAEPAPLSGGTLALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEC
EGTWVISSYSVASAIAVPLTGWLARRVGEVRLFTLSVLAFTIASALCGLAENFESLIAFR
CCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVAPIFGPVMGGWITDNYTWPW
HHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCE
IFYINLPIGMFSAACAFFLLRGRETKTTKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
EEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
NSTFITSLALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHEECCCCCHHHHHHHHHHHHHHHHHHHH
IFPLWLQTVMGYTAGLAGLATAPVGFLALVLSPLIGRNMHRLDLRMVASFAFVVFAGVSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
WNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSISDERLASASGLSNFLRTLSG
HCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AIGTAVSSTFWENDAIYHHARLAESVDVYAQSTIDFQGALARLGIVGDVSTAQINQIVTQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHH
QGFMMATNDFFHISALAFVALAALVWVTKPKKGAGPAMGH
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AATAPASASLSAEPAPLSGGTLALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEC
EGTWVISSYSVASAIAVPLTGWLARRVGEVRLFTLSVLAFTIASALCGLAENFESLIAFR
CCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVAPIFGPVMGGWITDNYTWPW
HHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCE
IFYINLPIGMFSAACAFFLLRGRETKTTKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
EEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
NSTFITSLALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHEECCCCCHHHHHHHHHHHHHHHHHHHH
IFPLWLQTVMGYTAGLAGLATAPVGFLALVLSPLIGRNMHRLDLRMVASFAFVVFAGVSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
WNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSISDERLASASGLSNFLRTLSG
HCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AIGTAVSSTFWENDAIYHHARLAESVDVYAQSTIDFQGALARLGIVGDVSTAQINQIVTQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHH
QGFMMATNDFFHISALAFVALAALVWVTKPKKGAGPAMGH
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]