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The map label for this gene is ispB [H]

Identifier: 83721521

GI number: 83721521

Start: 1285432

End: 1286424

Strand: Reverse

Name: ispB [H]

Synonym: BTH_I1139

Alternate gene names: 83721521

Gene position: 1286424-1285432 (Counterclockwise)

Preceding gene: 83718686

Following gene: 83721204

Centisome position: 33.77

GC content: 65.16

Gene sequence:

>993_bases
ATGTCGTCGACTGCCTCCCCCTCCCTCAGCGCCGCCCACCTGCTCACCCCGATCGCAAGCGACATGGAGCAGGTGAATCG
CGTCATCCGGCAAAGCCTCGCGTCCGACGTGCTGCTGATCAATCAGATCGCCGAGTACATCATCGGCGCGGGCGGCAAGC
GGCTGCGGCCGGCGCTTCTCCTGCTCGTCGCGGGCGCGCTCGGCGAACACACGAGCCAGAAGCACGTGCTCGCGGCCGTC
GTCGAATTCATCCATACGGCGACGCTGCTGCACGACGACGTCGTCGACGAATCCGAGCTGCGGCGCGGCCGCAAGACCGC
GAACGCGCTGTTCGGCAATGCGGCGAGCGTGCTCGTCGGCGATTACCTTTACTCGCGCTCGTTCGAGATGATGGTGGGCG
TCGGCAAGATGCGCGTGATGGAGATCCTGTCCGAGGCGACGACGATCATCTCCGAAGGCGAAGTGCTGCAACTCTTGAAC
ATGCACGATGCCGACGTCGACGAAGCCCGCTACATGCAGGTGATCCGCTACAAGACGGCGAAGCTGTTCGAGGCCGCCGC
GCGGCTCGGCGCGGTGCTCGCGGGCGCCGACGCGCCGACGGAAGCCGCCGCCGCCGAGTATGGCCGCCGCATCGGCACCG
CGTTCCAGATCATGGACGACTGGCTCGACTATGCGGGCACCGCCGAAGCGATGGGCAAGAACGCCGGCGACGATTTGCGC
GAAGGCAAGCCGACGCTGCCGCTCATCTATCTGATCGAACGCGGCACGCCCGAGCAGTCGACGCTTGCCCGCGAAGCGAT
CGAGCACGGCGGCACCGATCGCTTCGACACGATCTTCGACGCGATCACGCGCTCGGGCGCGCTCGACCATACGCTCGAAT
GCGCTCGCCAGGAGGCTCAAGCGGCCGCAGCAGCAATTTCCTCGTTCCCCCCTTCCATTTTCAAAGAGAGCCTGCTAGAA
TTATGTTTTTACTCGACGTCACGCCAGTCTTGA

Upstream 100 bases:

>100_bases
CATCGGCCGAACGGCCGAAAAACGCGCGATGACGTCGCACGACAGCCCGGCTCGACGGCGATTCGCCTTATAATCCGCGG
CACTACCCATTTCCATGATC

Downstream 100 bases:

>100_bases
TCTAGACCGAAGCGACGAATCAGTACGAATCGAGTTCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGA
CGTAGAGGCCGGAGGTTCGA

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSSTASPSLSAAHLLTPIASDMEQVNRVIRQSLASDVLLINQIAEYIIGAGGKRLRPALLLLVAGALGEHTSQKHVLAAV
VEFIHTATLLHDDVVDESELRRGRKTANALFGNAASVLVGDYLYSRSFEMMVGVGKMRVMEILSEATTIISEGEVLQLLN
MHDADVDEARYMQVIRYKTAKLFEAAARLGAVLAGADAPTEAAAAEYGRRIGTAFQIMDDWLDYAGTAEAMGKNAGDDLR
EGKPTLPLIYLIERGTPEQSTLAREAIEHGGTDRFDTIFDAITRSGALDHTLECARQEAQAAAAAISSFPPSIFKESLLE
LCFYSTSRQS

Sequences:

>Translated_330_residues
MSSTASPSLSAAHLLTPIASDMEQVNRVIRQSLASDVLLINQIAEYIIGAGGKRLRPALLLLVAGALGEHTSQKHVLAAV
VEFIHTATLLHDDVVDESELRRGRKTANALFGNAASVLVGDYLYSRSFEMMVGVGKMRVMEILSEATTIISEGEVLQLLN
MHDADVDEARYMQVIRYKTAKLFEAAARLGAVLAGADAPTEAAAAEYGRRIGTAFQIMDDWLDYAGTAEAMGKNAGDDLR
EGKPTLPLIYLIERGTPEQSTLAREAIEHGGTDRFDTIFDAITRSGALDHTLECARQEAQAAAAAISSFPPSIFKESLLE
LCFYSTSRQS
>Mature_329_residues
SSTASPSLSAAHLLTPIASDMEQVNRVIRQSLASDVLLINQIAEYIIGAGGKRLRPALLLLVAGALGEHTSQKHVLAAVV
EFIHTATLLHDDVVDESELRRGRKTANALFGNAASVLVGDYLYSRSFEMMVGVGKMRVMEILSEATTIISEGEVLQLLNM
HDADVDEARYMQVIRYKTAKLFEAAARLGAVLAGADAPTEAAAAEYGRRIGTAFQIMDDWLDYAGTAEAMGKNAGDDLRE
GKPTLPLIYLIERGTPEQSTLAREAIEHGGTDRFDTIFDAITRSGALDHTLECARQEAQAAAAAISSFPPSIFKESLLEL
CFYSTSRQS

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=308, Percent_Identity=29.5454545454545, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI4758430, Length=209, Percent_Identity=23.9234449760766, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI83700220, Length=209, Percent_Identity=23.9234449760766, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789578, Length=311, Percent_Identity=50.8038585209003, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1786623, Length=243, Percent_Identity=32.0987654320988, Blast_Score=91, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=321, Percent_Identity=31.4641744548287, Blast_Score=151, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6319475, Length=242, Percent_Identity=30.1652892561983, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6325188, Length=236, Percent_Identity=24.1525423728814, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24651612, Length=287, Percent_Identity=31.7073170731707, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI281365769, Length=263, Percent_Identity=25.8555133079848, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24660002, Length=263, Percent_Identity=25.8555133079848, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35647; Mature: 35516

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTASPSLSAAHLLTPIASDMEQVNRVIRQSLASDVLLINQIAEYIIGAGGKRLRPALL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LLVAGALGEHTSQKHVLAAVVEFIHTATLLHDDVVDESELRRGRKTANALFGNAASVLVG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHH
DYLYSRSFEMMVGVGKMRVMEILSEATTIISEGEVLQLLNMHDADVDEARYMQVIRYKTA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
KLFEAAARLGAVLAGADAPTEAAAAEYGRRIGTAFQIMDDWLDYAGTAEAMGKNAGDDLR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHH
EGKPTLPLIYLIERGTPEQSTLAREAIEHGGTDRFDTIFDAITRSGALDHTLECARQEAQ
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
AAAAAISSFPPSIFKESLLELCFYSTSRQS
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSTASPSLSAAHLLTPIASDMEQVNRVIRQSLASDVLLINQIAEYIIGAGGKRLRPALL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LLVAGALGEHTSQKHVLAAVVEFIHTATLLHDDVVDESELRRGRKTANALFGNAASVLVG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHH
DYLYSRSFEMMVGVGKMRVMEILSEATTIISEGEVLQLLNMHDADVDEARYMQVIRYKTA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
KLFEAAARLGAVLAGADAPTEAAAAEYGRRIGTAFQIMDDWLDYAGTAEAMGKNAGDDLR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHH
EGKPTLPLIYLIERGTPEQSTLAREAIEHGGTDRFDTIFDAITRSGALDHTLECARQEAQ
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
AAAAAISSFPPSIFKESLLELCFYSTSRQS
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]