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The map label for this gene is pgk [H]

Identifier: 83721516

GI number: 83721516

Start: 760275

End: 761468

Strand: Direct

Name: pgk [H]

Synonym: BTH_I0663

Alternate gene names: 83721516

Gene position: 760275-761468 (Clockwise)

Preceding gene: 83719146

Following gene: 284159404

Centisome position: 19.96

GC content: 68.34

Gene sequence:

>1194_bases
ATGAGCCAAGTAAAGCGTCTTACCGACCTGATCGCCGAAGGCAAGCTCTCCGGCAAACGTGTGTTCATCCGCGCCGATCT
GAACGTGCCGCAGGACGATCACGGCAACATCACCGAAGACACGCGCGTGCGCGCGTCGGTGCCCGCGATCCAGGCGGCGC
TCGACGCGGGCGCGGCCGTGATGGTCACGTCGCACCTCGGCCGCCCGACGGAAGGCGAGTTCACGCCCGAGGATTCGCTC
GCGCCCGTCGCGAAGCGCCTCGCCGAGCTGCTCGGCCGCGACGTGCCGCTCGTCGCGAACTGGGTCGAGAACGGCGTCGA
GGTTGCGCCCGGGCAGGTCGTGCTGCTCGAGAACTGCCGCGTGAACAAGGGCGAGAAGAAGAACTCGGACGAGCTCGCGC
AGAAGATGGCGAAGCTCTGCGACGTCTACGTGAACGACGCGTTCGGCACCGCGCACCGCGCGGAGGCGACCACGCACGGC
ATCGCGAAGTACGCCCCCGTCGCGTGCGCGGGCCCGCTGCTCGCCGCGGAGCTCGACGCGCTCGGCAAGGCGCTCGGCAA
CCCGAAGCGCCCGCTGGTGGCGATCGTCGCGGGCTCGAAGGTGTCGACGAAGCTGACGATCCTGAAGTCGCTCGCGGAGA
AGGTCGATCAACTGATCGTCGGCGGCGGCATCGCGAACACGTTCATGCTCGCGGCGGGCCTCGCGATCGGCAAGTCGCTC
GCGGAGGCCGATCTCGTCGGCGAGGCGAAGGCGATCATCGACGAAGCCCGCAAGCGCGGCGCGTCGGTGCCGATCCCGAC
CGACGTCGTCGTCGCGAAGGAATTCTCGCCGACGGCCGTCGCCACCGTGAAGAAGGTCGCCGACATCGAAGCCGACGACA
TGATCCTCGACATCGGGCCGGAAACCGCGAAGGCGCTCGCCGGCCAGCTCGAGAAGGCGGGCACGATCGTCTGGAACGGC
CCCGTCGGCGTGTTCGAGTTCGACCAGTTCGGCAACGGCACGAAGACGCTCGCCGACGCGATCGCGAATTCGGCCGCGTT
CTCGATCGCGGGCGGCGGCGACACGCTCGCCGCGATCGCGAAGTACGGGATTCACGACAAGGTCAGCTACATCTCGACGG
GCGGCGGCGCGTTCCTCGAATTCCTCGAGGGCAAGAAGCTGCCCGCCGTCGAAGTGCTGGAAACGCGCGCGTAA

Upstream 100 bases:

>100_bases
CGCGGGCCCTGCCGCGCGAAGCCGGCGGGCCGCGCGAGGCGCCCGGCGCGGCAGCCCGCGCGCGTCGAACCGCCTTCCTT
CATCAACCTTCATCAGTCCC

Downstream 100 bases:

>100_bases
GCACGCGACATCGCGCGCGGCCGCGAGCAGGTGCCGGCGGCGCGCGATAAACGCGGGCGGCAGCCGCCCGCGTGACGCCG
GCGGGCACGCGCGTTGCGCG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSQVKRLTDLIAEGKLSGKRVFIRADLNVPQDDHGNITEDTRVRASVPAIQAALDAGAAVMVTSHLGRPTEGEFTPEDSL
APVAKRLAELLGRDVPLVANWVENGVEVAPGQVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHG
IAKYAPVACAGPLLAAELDALGKALGNPKRPLVAIVAGSKVSTKLTILKSLAEKVDQLIVGGGIANTFMLAAGLAIGKSL
AEADLVGEAKAIIDEARKRGASVPIPTDVVVAKEFSPTAVATVKKVADIEADDMILDIGPETAKALAGQLEKAGTIVWNG
PVGVFEFDQFGNGTKTLADAIANSAAFSIAGGGDTLAAIAKYGIHDKVSYISTGGGAFLEFLEGKKLPAVEVLETRA

Sequences:

>Translated_397_residues
MSQVKRLTDLIAEGKLSGKRVFIRADLNVPQDDHGNITEDTRVRASVPAIQAALDAGAAVMVTSHLGRPTEGEFTPEDSL
APVAKRLAELLGRDVPLVANWVENGVEVAPGQVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHG
IAKYAPVACAGPLLAAELDALGKALGNPKRPLVAIVAGSKVSTKLTILKSLAEKVDQLIVGGGIANTFMLAAGLAIGKSL
AEADLVGEAKAIIDEARKRGASVPIPTDVVVAKEFSPTAVATVKKVADIEADDMILDIGPETAKALAGQLEKAGTIVWNG
PVGVFEFDQFGNGTKTLADAIANSAAFSIAGGGDTLAAIAKYGIHDKVSYISTGGGAFLEFLEGKKLPAVEVLETRA
>Mature_396_residues
SQVKRLTDLIAEGKLSGKRVFIRADLNVPQDDHGNITEDTRVRASVPAIQAALDAGAAVMVTSHLGRPTEGEFTPEDSLA
PVAKRLAELLGRDVPLVANWVENGVEVAPGQVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGI
AKYAPVACAGPLLAAELDALGKALGNPKRPLVAIVAGSKVSTKLTILKSLAEKVDQLIVGGGIANTFMLAAGLAIGKSLA
EADLVGEAKAIIDEARKRGASVPIPTDVVVAKEFSPTAVATVKKVADIEADDMILDIGPETAKALAGQLEKAGTIVWNGP
VGVFEFDQFGNGTKTLADAIANSAAFSIAGGGDTLAAIAKYGIHDKVSYISTGGGAFLEFLEGKKLPAVEVLETRA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=423, Percent_Identity=42.5531914893617, Blast_Score=283, Evalue=3e-76,
Organism=Homo sapiens, GI4505763, Length=421, Percent_Identity=40.3800475059382, Blast_Score=273, Evalue=3e-73,
Organism=Escherichia coli, GI1789294, Length=397, Percent_Identity=63.727959697733, Blast_Score=473, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17508823, Length=419, Percent_Identity=43.436754176611, Blast_Score=300, Evalue=7e-82,
Organism=Saccharomyces cerevisiae, GI6319857, Length=408, Percent_Identity=43.3823529411765, Blast_Score=283, Evalue=4e-77,
Organism=Drosophila melanogaster, GI17136394, Length=406, Percent_Identity=40.3940886699507, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24581203, Length=421, Percent_Identity=38.2422802850356, Blast_Score=245, Evalue=3e-65,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41245; Mature: 41114

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVKRLTDLIAEGKLSGKRVFIRADLNVPQDDHGNITEDTRVRASVPAIQAALDAGAAV
CCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEE
MVTSHLGRPTEGEFTPEDSLAPVAKRLAELLGRDVPLVANWVENGVEVAPGQVVLLENCR
EEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCEECCCCEEEEECCC
VNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGIAKYAPVACAGPLLAAELDA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHH
LGKALGNPKRPLVAIVAGSKVSTKLTILKSLAEKVDQLIVGGGIANTFMLAAGLAIGKSL
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AEADLVGEAKAIIDEARKRGASVPIPTDVVVAKEFSPTAVATVKKVADIEADDMILDIGP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCEEEECCC
ETAKALAGQLEKAGTIVWNGPVGVFEFDQFGNGTKTLADAIANSAAFSIAGGGDTLAAIA
HHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHH
KYGIHDKVSYISTGGGAFLEFLEGKKLPAVEVLETRA
HHCCCCCHHEEECCCHHHHHHHCCCCCCHHHHHHCCC
>Mature Secondary Structure 
SQVKRLTDLIAEGKLSGKRVFIRADLNVPQDDHGNITEDTRVRASVPAIQAALDAGAAV
CHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEE
MVTSHLGRPTEGEFTPEDSLAPVAKRLAELLGRDVPLVANWVENGVEVAPGQVVLLENCR
EEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCEECCCCEEEEECCC
VNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGIAKYAPVACAGPLLAAELDA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHH
LGKALGNPKRPLVAIVAGSKVSTKLTILKSLAEKVDQLIVGGGIANTFMLAAGLAIGKSL
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AEADLVGEAKAIIDEARKRGASVPIPTDVVVAKEFSPTAVATVKKVADIEADDMILDIGP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCEEEECCC
ETAKALAGQLEKAGTIVWNGPVGVFEFDQFGNGTKTLADAIANSAAFSIAGGGDTLAAIA
HHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHH
KYGIHDKVSYISTGGGAFLEFLEGKKLPAVEVLETRA
HHCCCCCHHEEECCCHHHHHHHCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]