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The map label for this gene is pyk-1 [H]

Identifier: 284159404

GI number: 284159404

Start: 761540

End: 763234

Strand: Direct

Name: pyk-1 [H]

Synonym: BTH_I0664

Alternate gene names: 284159404

Gene position: 761540-763234 (Clockwise)

Preceding gene: 83721516

Following gene: 83719899

Centisome position: 19.99

GC content: 68.97

Gene sequence:

>1695_bases
GTGACGCCGGCGGGCACGCGCGTTGCGCGCCTCGCCGGCCCGGCGGCTTTCGTCAGGCCGTCAAGCGGTGCGCCCGGGGC
GGGCGCGCCGAACCCGAATCAACGACGCGGCGCCGGCCGTTCACGCCGGCGCCCGCGGACAAAGCGACGCCGGGCGCACG
CACCGGCGCGCGTTCCGAAGCGGCGGCGGCTGGGCCGCGCGGCTTCGGCTCTCGCGGCGTTACATCCAGCGCTTTCACTC
AGTCAGACGAGGAGATTCATGCATCGCGCCACCAAGATAGTCGCCACGATCGGTCCGGCATCCAGCTCGCCGGAGGTCCT
GTTGCAGATGATGCACGCGGGTCTCGACGTCGTGCGGCTCAATTTCTCGCACGGCTCCGCCGACGATCACCGCGAGCGGG
CCGAGATGGTCCGCGAGGCCGCGCGCCGGGTCGGCCGGGAAATCGCGATCATGGCGGACCTGCAGGGGCCGAAGATCCGC
GTCGGCAAGTTCGAGGCGGGCAAGACGACGCTCACGCCCGGCCAGCCGTTCATCCTCGACGCGACGTGCGAGCTCGGCAA
CGACGAGCGGGTCGGCCTCGACTACAAGGATCTGCCGCGCGACCTGAAGCCGGGCGACGTGCTGCTGCTTAACGACGGCC
TGATCGTGCTGAAGGTCGACCGCGTGTTCGGCGATGAGATTCACACGACCGTCAAGGTGGGCGGCGATCTGTCGAACAAC
AAGGGGATCAACCGGCAGGGCGGCGGGCTGTCGGCGCCCGCGCTGACCGCGAAGGACATGGAGGACATCCGCACCGCGAT
GTCGCTCGGCGCGGATCTCGTCGCCGTGTCGTTCCCGAAGAACGCGACCGACATGGAGATGGCGCGCCAGCTCGCGAACA
TCGCGGGTGCGCCTTACGGCATCAAGCCGAAGATGATCGCGAAGATCGAGCGCGCCGAGGCGATTCCGGCGCTGCAGGAG
ATTCTCGACGCGTCGGACGGCATCATGGTCGCGCGCGGCGATTTGGCCGTCGAGGTCGGCAACGCGGCCGTGCCCGCGCT
GCAGAAGCGGATGATCCGGATGGCGCGCGAGTCGAACAAGCTCGTGATCACCGCGACGCAGATGATGGAATCGATGATCC
ACGCGCCCGTGCCGACGCGCGCGGAGGTGTCGGACGTCGCGAACGCCGTGCTCGACGGCACCGATGCGGTGATGCTGTCG
GCCGAGACGGCGACGGGCAAGTATCCGGCCGTGACGATCGAGGCGATGGCGGCGATCTGCGTCGAGGCGGAGAAATCCGA
GCACGTCGAGCTCGACAAGGATTTCCTCGACCGCACGTTCACGCGGATCGACCAGTCGATCGCGATGGGCGCGCTCTTCA
CCGCGTACCACCTGGGCGCGAAGGCGATCGTCGCGCTGACCGAATCGGGCGCGACGGCGCTCTGGATGTCGCGCCACTAC
ACGCACGTGCCGATCTTCGCGCTGACGCCGCGAGTCGGCAGCGAGCGCGCGATGGCGCTCTTTCGCAACGTGACGCCGCT
GCACGTCGACTTCGACAGCGATCGCGATTCCGCGCTGCAGCAGGCGCTCGAGCTGATCGTGCGGCGCGGCTACGTCGCGC
ATGGCGACATGGTCGTGCTGACGGTCGGCGAGCCGATGGGGCAGGCGGGCGGCACGAACACGCTGAAGATCGTGCGCGTC
GGCGAGCACTACTGA

Upstream 100 bases:

>100_bases
CCGTCGAAGTGCTGGAAACGCGCGCGTAAGCACGCGACATCGCGCGCGGCCGCGAGCAGGTGCCGGCGGCGCGCGATAAA
CGCGGGCGGCAGCCGCCCGC

Downstream 100 bases:

>100_bases
AAGCGGCCGGATGCCCGAGCGGCGCGTTCGCTCGCCGGGCATGCCGCTTGCGCGGTCACGAGGAAGCCGCGGGGTCCGAC
GCCCCGCGGCTTTTTTCGCT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MTPAGTRVARLAGPAAFVRPSSGAPGAGAPNPNQRRGAGRSRRRPRTKRRRAHAPARVPKRRRLGRAASALAALHPALSL
SQTRRFMHRATKIVATIGPASSSPEVLLQMMHAGLDVVRLNFSHGSADDHRERAEMVREAARRVGREIAIMADLQGPKIR
VGKFEAGKTTLTPGQPFILDATCELGNDERVGLDYKDLPRDLKPGDVLLLNDGLIVLKVDRVFGDEIHTTVKVGGDLSNN
KGINRQGGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQE
ILDASDGIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLS
AETATGKYPAVTIEAMAAICVEAEKSEHVELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIVALTESGATALWMSRHY
THVPIFALTPRVGSERAMALFRNVTPLHVDFDSDRDSALQQALELIVRRGYVAHGDMVVLTVGEPMGQAGGTNTLKIVRV
GEHY

Sequences:

>Translated_564_residues
MTPAGTRVARLAGPAAFVRPSSGAPGAGAPNPNQRRGAGRSRRRPRTKRRRAHAPARVPKRRRLGRAASALAALHPALSL
SQTRRFMHRATKIVATIGPASSSPEVLLQMMHAGLDVVRLNFSHGSADDHRERAEMVREAARRVGREIAIMADLQGPKIR
VGKFEAGKTTLTPGQPFILDATCELGNDERVGLDYKDLPRDLKPGDVLLLNDGLIVLKVDRVFGDEIHTTVKVGGDLSNN
KGINRQGGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQE
ILDASDGIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLS
AETATGKYPAVTIEAMAAICVEAEKSEHVELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIVALTESGATALWMSRHY
THVPIFALTPRVGSERAMALFRNVTPLHVDFDSDRDSALQQALELIVRRGYVAHGDMVVLTVGEPMGQAGGTNTLKIVRV
GEHY
>Mature_563_residues
TPAGTRVARLAGPAAFVRPSSGAPGAGAPNPNQRRGAGRSRRRPRTKRRRAHAPARVPKRRRLGRAASALAALHPALSLS
QTRRFMHRATKIVATIGPASSSPEVLLQMMHAGLDVVRLNFSHGSADDHRERAEMVREAARRVGREIAIMADLQGPKIRV
GKFEAGKTTLTPGQPFILDATCELGNDERVGLDYKDLPRDLKPGDVLLLNDGLIVLKVDRVFGDEIHTTVKVGGDLSNNK
GINRQGGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQEI
LDASDGIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSA
ETATGKYPAVTIEAMAAICVEAEKSEHVELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIVALTESGATALWMSRHYT
HVPIFALTPRVGSERAMALFRNVTPLHVDFDSDRDSALQQALELIVRRGYVAHGDMVVLTVGEPMGQAGGTNTLKIVRVG
EHY

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=493, Percent_Identity=36.9168356997972, Blast_Score=280, Evalue=4e-75,
Organism=Homo sapiens, GI33286420, Length=493, Percent_Identity=36.9168356997972, Blast_Score=280, Evalue=4e-75,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=37.5, Blast_Score=278, Evalue=9e-75,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=37.5, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=36.5111561866126, Blast_Score=275, Evalue=7e-74,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=36.426116838488, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=36.426116838488, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=33.4728033472803, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=33.4728033472803, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=31.1926605504587, Blast_Score=106, Evalue=7e-23,
Organism=Escherichia coli, GI1788160, Length=484, Percent_Identity=51.8595041322314, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1787965, Length=468, Percent_Identity=39.5299145299145, Blast_Score=305, Evalue=7e-84,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=36.8852459016393, Blast_Score=284, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI17506831, Length=558, Percent_Identity=37.0967741935484, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI71984413, Length=540, Percent_Identity=36.8518518518519, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17506829, Length=540, Percent_Identity=36.8518518518519, Blast_Score=265, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=38.6036960985626, Blast_Score=264, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6324923, Length=492, Percent_Identity=36.3821138211382, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6319279, Length=490, Percent_Identity=34.6938775510204, Blast_Score=254, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24648964, Length=498, Percent_Identity=38.7550200803213, Blast_Score=284, Evalue=9e-77,
Organism=Drosophila melanogaster, GI28571814, Length=498, Percent_Identity=38.7550200803213, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24648966, Length=412, Percent_Identity=37.621359223301, Blast_Score=238, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24581235, Length=489, Percent_Identity=31.9018404907975, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=37.1323529411765, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 60621; Mature: 60490

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAGTRVARLAGPAAFVRPSSGAPGAGAPNPNQRRGAGRSRRRPRTKRRRAHAPARVPK
CCCCCCHHHHHCCCCCEECCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHCCCCCCCCH
RRRLGRAASALAALHPALSLSQTRRFMHRATKIVATIGPASSSPEVLLQMMHAGLDVVRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCEEEEE
NFSHGSADDHRERAEMVREAARRVGREIAIMADLQGPKIRVGKFEAGKTTLTPGQPFILD
ECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCEECCCCCCEEEE
ATCELGNDERVGLDYKDLPRDLKPGDVLLLNDGLIVLKVDRVFGDEIHTTVKVGGDLSNN
EEECCCCCCCCCCCHHHCCCCCCCCCEEEEECCEEEEEEHHHHCCCCEEEEEECCCCCCC
KGINRQGGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCC
IKPKMIAKIERAEAIPALQEILDASDGIMVARGDLAVEVGNAAVPALQKRMIRMARESNK
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCC
LVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAETATGKYPAVTIEAMAAIC
EEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEHHHHHHHHH
VEAEKSEHVELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIVALTESGATALWMSRHY
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCC
THVPIFALTPRVGSERAMALFRNVTPLHVDFDSDRDSALQQALELIVRRGYVAHGDMVVL
CCCEEEEECCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCEECCCEEEE
TVGEPMGQAGGTNTLKIVRVGEHY
ECCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
TPAGTRVARLAGPAAFVRPSSGAPGAGAPNPNQRRGAGRSRRRPRTKRRRAHAPARVPK
CCCCCHHHHHCCCCCEECCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHCCCCCCCCH
RRRLGRAASALAALHPALSLSQTRRFMHRATKIVATIGPASSSPEVLLQMMHAGLDVVRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCEEEEE
NFSHGSADDHRERAEMVREAARRVGREIAIMADLQGPKIRVGKFEAGKTTLTPGQPFILD
ECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCEECCCCCCEEEE
ATCELGNDERVGLDYKDLPRDLKPGDVLLLNDGLIVLKVDRVFGDEIHTTVKVGGDLSNN
EEECCCCCCCCCCCHHHCCCCCCCCCEEEEECCEEEEEEHHHHCCCCEEEEEECCCCCCC
KGINRQGGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCC
IKPKMIAKIERAEAIPALQEILDASDGIMVARGDLAVEVGNAAVPALQKRMIRMARESNK
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCC
LVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAETATGKYPAVTIEAMAAIC
EEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEHHHHHHHHH
VEAEKSEHVELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIVALTESGATALWMSRHY
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCC
THVPIFALTPRVGSERAMALFRNVTPLHVDFDSDRDSALQQALELIVRRGYVAHGDMVVL
CCCEEEEECCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCEECCCEEEE
TVGEPMGQAGGTNTLKIVRVGEHY
ECCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]