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The map label for this gene is dctD [H]

Identifier: 83721461

GI number: 83721461

Start: 446210

End: 447565

Strand: Direct

Name: dctD [H]

Synonym: BTH_I0401

Alternate gene names: 83721461

Gene position: 446210-447565 (Clockwise)

Preceding gene: 83721206

Following gene: 83719684

Centisome position: 11.71

GC content: 69.91

Gene sequence:

>1356_bases
ATGGCGAACCGCCTGCAAGTGATCTACATCGAAGACGACGAGCTCGTGCGCCGCGCGAGCGTGCAGAGCCTGCAGCTCGC
GGGCTTCGACGTCGTCGGGTTCGGTTCGGTCGAGGCGGCCGAGAAGGCGATCGTCGGCGACGCGGCGGGCGTGATCGTGT
CCGACATCCGCCTGCCCGGCGCGAGCGGCCTCGATCTGCTCGCGCAATGTCGCGCACGCACGCCCGACGTGCCCGTCGTG
CTCGTCACCGGGCACGGCGACATCTCGATGGCCGTGCAGGCGATGCGCGACGGCGCTTACGACTTCATCGAAAAGCCATT
CGCGGCCGAGCGCCTGATCGAGACGGCGCGGCGCGCGCTCGAGCGCCGCGCGCTCGTGCTCGAGAACCACGCGCTGCGGC
GCGAGCTCGCGGGGCAGGGCGTCGTCGCGCCGCGGATCATCGGCCGCAGCCCGGCGATCGAGCAGGTGCGGCGGCTGATC
GCGAACGTCGCGCCGACCGACGCGTCGGTCCTCATCAACGGCGACACCGGCGCCGGCAAGGAACTGATCGCGCGCAGCTT
GCACGAGCTGTCGCCGCGCCGCGACAAGCCGTTCATCGCGGTCAACTGCGGCGCGCTGCCCGAGCCGATGTTCGAATCCG
AGATGTTCGGCTACGAGCCGGGCGCGTTTACCGGCGCGGCGAAGCGCCGTATCGGCAAGCTCGAATATGCGTCAGGCGGC
ACGCTGTTCCTCGACGAGATCGAGAGCATGCCGCTCGCGCTGCAGGTGAAGCTGTTGCGCGTGCTGCAGGACGGCGTGCT
CGAGCGGCTCGGCTCGAACCAGCCGATCCGCGTGAACTGCCGCGTGGTCGCGGCGGCGAAGGGCGACATGAGCGAGCATG
TCGCGGCCGGCACGTTCCGGCGCGACCTGCTGTACCGGCTGAATGTCGTGACGATCGCGCTGCCGCCGCTCGCCGAGCGC
CGCGAGGACATCGTGCCGCTCTTCGAGCACTTCATGCTCGACGCGGCGGTCCGTTACGGGCGTCCGGCTCCGCTCCTCAC
CGATCGTCAGCGCGCGAGCCTGATGCAGCGCGACTGGCCGGGCAACGTGCGCGAGCTGCGCAATGCGGCCGATCGCTTCG
TGCTCGGCGTGACGGAAGGCTTCGTCGGCGATGCGGGCGGGCCAACCGACGAGAGCGCCGAGCAGTCGCTCAAGGAGCGC
GTCGAGCAATTCGAGCGCGCGGTGATCGCCGAGACGCTGAACCGCACGGGTGGCGCGGTCGCGACGACGGCCGACAAGCT
GCATGTCGGCAAGGCGACGCTCTACGAGAAGATGAAGCGCTATGGGCTGAGCGCGAAAGGAGAAACGGAGCGCTGA

Upstream 100 bases:

>100_bases
GACGCTGCGCCGCGCGCGCACGCATCATCCGGACACTTCCGCCGCGAGCTGAGCGCGCGTTTCGCGCGCGGCCCGCGTCA
GCATCATGGGGTGACAGAAG

Downstream 100 bases:

>100_bases
TGCGGGCGTGGCCCGCGTCGAGCGATCCGTTCGGAGGGCGGCGGCGCGCCGTGCGGCGGGCCGCCGAGACGAAGGGCGCG
CTTACGCGCTCGTGTCGAGC

Product: C4-dicarboxylate transport transcriptional regulatory protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MANRLQVIYIEDDELVRRASVQSLQLAGFDVVGFGSVEAAEKAIVGDAAGVIVSDIRLPGASGLDLLAQCRARTPDVPVV
LVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETARRALERRALVLENHALRRELAGQGVVAPRIIGRSPAIEQVRRLI
ANVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGG
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSEHVAAGTFRRDLLYRLNVVTIALPPLAER
REDIVPLFEHFMLDAAVRYGRPAPLLTDRQRASLMQRDWPGNVRELRNAADRFVLGVTEGFVGDAGGPTDESAEQSLKER
VEQFERAVIAETLNRTGGAVATTADKLHVGKATLYEKMKRYGLSAKGETER

Sequences:

>Translated_451_residues
MANRLQVIYIEDDELVRRASVQSLQLAGFDVVGFGSVEAAEKAIVGDAAGVIVSDIRLPGASGLDLLAQCRARTPDVPVV
LVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETARRALERRALVLENHALRRELAGQGVVAPRIIGRSPAIEQVRRLI
ANVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGG
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSEHVAAGTFRRDLLYRLNVVTIALPPLAER
REDIVPLFEHFMLDAAVRYGRPAPLLTDRQRASLMQRDWPGNVRELRNAADRFVLGVTEGFVGDAGGPTDESAEQSLKER
VEQFERAVIAETLNRTGGAVATTADKLHVGKATLYEKMKRYGLSAKGETER
>Mature_450_residues
ANRLQVIYIEDDELVRRASVQSLQLAGFDVVGFGSVEAAEKAIVGDAAGVIVSDIRLPGASGLDLLAQCRARTPDVPVVL
VTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETARRALERRALVLENHALRRELAGQGVVAPRIIGRSPAIEQVRRLIA
NVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGGT
LFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSEHVAAGTFRRDLLYRLNVVTIALPPLAERR
EDIVPLFEHFMLDAAVRYGRPAPLLTDRQRASLMQRDWPGNVRELRNAADRFVLGVTEGFVGDAGGPTDESAEQSLKERV
EQFERAVIAETLNRTGGAVATTADKLHVGKATLYEKMKRYGLSAKGETER

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=469, Percent_Identity=37.5266524520256, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1788550, Length=452, Percent_Identity=37.1681415929204, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1790437, Length=441, Percent_Identity=37.6417233560091, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1788905, Length=449, Percent_Identity=36.7483296213808, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=41.5625, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI87082117, Length=346, Percent_Identity=41.6184971098266, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1789087, Length=327, Percent_Identity=43.4250764525994, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=41.7956656346749, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1786524, Length=323, Percent_Identity=39.0092879256966, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=47.3913043478261, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1787583, Length=329, Percent_Identity=37.3860182370821, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI87081858, Length=371, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=35.655737704918, Blast_Score=131, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49123; Mature: 48992

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRLQVIYIEDDELVRRASVQSLQLAGFDVVGFGSVEAAEKAIVGDAAGVIVSDIRLPG
CCCCEEEEEECCHHHHHHHHHHHHEECCCEEEECCCHHHHHHHHHCCCCCHHEEHEECCC
ASGLDLLAQCRARTPDVPVVLVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETARRAL
CCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
ERRALVLENHALRRELAGQGVVAPRIIGRSPAIEQVRRLIANVAPTDASVLINGDTGAGK
HHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
ELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGG
HHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHCCEEECCCC
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSEHVAAGTFR
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHHH
RDLLYRLNVVTIALPPLAERREDIVPLFEHFMLDAAVRYGRPAPLLTDRQRASLMQRDWP
HHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
GNVRELRNAADRFVLGVTEGFVGDAGGPTDESAEQSLKERVEQFERAVIAETLNRTGGAV
CCHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ATTADKLHVGKATLYEKMKRYGLSAKGETER
EECHHHHHCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ANRLQVIYIEDDELVRRASVQSLQLAGFDVVGFGSVEAAEKAIVGDAAGVIVSDIRLPG
CCCEEEEEECCHHHHHHHHHHHHEECCCEEEECCCHHHHHHHHHCCCCCHHEEHEECCC
ASGLDLLAQCRARTPDVPVVLVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETARRAL
CCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
ERRALVLENHALRRELAGQGVVAPRIIGRSPAIEQVRRLIANVAPTDASVLINGDTGAGK
HHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
ELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGG
HHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHCCEEECCCC
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSEHVAAGTFR
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHHH
RDLLYRLNVVTIALPPLAERREDIVPLFEHFMLDAAVRYGRPAPLLTDRQRASLMQRDWP
HHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
GNVRELRNAADRFVLGVTEGFVGDAGGPTDESAEQSLKERVEQFERAVIAETLNRTGGAV
CCHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ATTADKLHVGKATLYEKMKRYGLSAKGETER
EECHHHHHCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]