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The map label for this gene is truB [H]

Identifier: 83721451

GI number: 83721451

Start: 2925268

End: 2926203

Strand: Reverse

Name: truB [H]

Synonym: BTH_I2562

Alternate gene names: 83721451

Gene position: 2926203-2925268 (Counterclockwise)

Preceding gene: 161723141

Following gene: 83720444

Centisome position: 76.82

GC content: 70.41

Gene sequence:

>936_bases
ATGACGACTGTTTCGCCCCGCCCCCGGATGGCTCGCCGCGCGCTCGACGGCGTGCTGCTGCTCGACAAGCCGGTCGGGCT
TTCGAGCAACGACGCGCTGATGCGTGCGAAGCGTTTGTACCTTGCGAAGAAAGCGGGCCACACGGGCACGCTCGATCCGC
TCGCATCGGGGCTGCTGCCGCTTTGCTTCGGCGAGGCGACGAAGTTCTCGCAGGATCTGCTCGATGCCGACAAGACCTAC
GAAGCGACGATGCGGCTCGGCGTGCGCACGACGACGGGCGACGCCGAGGGCGACGTGCTCGACACGCGCGACGTGGCCTG
CGACGAAGCGACGGTCGGCGCGGCGCTTGCGCGCTTCGTCGGCGATATCGTGCAGGTGCCGCCGATGTACTCGGCGCTCA
AGCGCGACGGCAAGCCGCTCTACGAATATGCGCGCGCCGGCCAGACGGTCGAGCGCGAGGGCCGCACGGTGACGATCCGC
TCGCTCGCGCTCCTGTCGTGCGCGTTGCCCGACGTCACGTTTCGTGTCACGTGCAGCAAGGGCACGTATGTGCGCACGCT
CGCCGAGGATATCGGCGAAGCGCTCGGTTGCGGCGCGCATCTGACGATGTTGCGCCGCACCGGCGTCGGCTCGCTGACGC
TCGAGCATGCGGTGACGCTCGACGCGCTCGACGCCGCGACGCAGGAAGAGCGCGACGCGCGGCTCGCGCCCGTCGACGCG
CTGCTGTCGACATTCCCCCTCGTGAGGCTCGACGCGGCGCTCACGAAGCGCTTCCTGCACGGCCAGCGGCTGAAGCTCGC
CGAGCTCGCGGCGCGACCCGAGGCGGACGAAGGCGAGCGCGTGCGCGTCTATGATGCCGATGACCGGCTGCTCGGCGTCG
CGCGCGCGTCGGAAGGCGTGCTTGCCCCGGAGCGCCTCGTCGTGACGGGCGCGTAG

Upstream 100 bases:

>100_bases
AGACGATCGAGAAGGCCGTCGCGATGTCGCGCCTCATCGACGAGGCGAACGCGACGCGCGCGAAGGACGACTGACCAACG
AACGAGCCTTGGCGCAGACC

Downstream 100 bases:

>100_bases
TCGCGCGGATGCACGGGCGCGCGGCGTGGATGCGCGCTCGCATGGCGCATGCTGTACGGCAGGCGTTGCGGACGACCGGC
GCGCTCGGCGCCGCATGTCG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTTVSPRPRMARRALDGVLLLDKPVGLSSNDALMRAKRLYLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLDADKTY
EATMRLGVRTTTGDAEGDVLDTRDVACDEATVGAALARFVGDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRTVTIR
SLALLSCALPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGSLTLEHAVTLDALDAATQEERDARLAPVDA
LLSTFPLVRLDAALTKRFLHGQRLKLAELAARPEADEGERVRVYDADDRLLGVARASEGVLAPERLVVTGA

Sequences:

>Translated_311_residues
MTTVSPRPRMARRALDGVLLLDKPVGLSSNDALMRAKRLYLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLDADKTY
EATMRLGVRTTTGDAEGDVLDTRDVACDEATVGAALARFVGDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRTVTIR
SLALLSCALPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGSLTLEHAVTLDALDAATQEERDARLAPVDA
LLSTFPLVRLDAALTKRFLHGQRLKLAELAARPEADEGERVRVYDADDRLLGVARASEGVLAPERLVVTGA
>Mature_310_residues
TTVSPRPRMARRALDGVLLLDKPVGLSSNDALMRAKRLYLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLDADKTYE
ATMRLGVRTTTGDAEGDVLDTRDVACDEATVGAALARFVGDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRTVTIRS
LALLSCALPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGSLTLEHAVTLDALDAATQEERDARLAPVDAL
LSTFPLVRLDAALTKRFLHGQRLKLAELAARPEADEGERVRVYDADDRLLGVARASEGVLAPERLVVTGA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=250, Percent_Identity=36.4, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI215599015, Length=213, Percent_Identity=31.4553990610329, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4503337, Length=213, Percent_Identity=31.4553990610329, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367200, Length=317, Percent_Identity=45.4258675078864, Blast_Score=256, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17553978, Length=271, Percent_Identity=29.520295202952, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324037, Length=196, Percent_Identity=34.1836734693878, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323204, Length=308, Percent_Identity=28.8961038961039, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281364189, Length=259, Percent_Identity=26.6409266409266, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364187, Length=259, Percent_Identity=26.6409266409266, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364185, Length=259, Percent_Identity=26.6409266409266, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364183, Length=259, Percent_Identity=26.6409266409266, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI62471759, Length=259, Percent_Identity=26.6409266409266, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17975520, Length=259, Percent_Identity=26.6409266409266, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33419; Mature: 33288

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVSPRPRMARRALDGVLLLDKPVGLSSNDALMRAKRLYLAKKAGHTGTLDPLASGLLP
CCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCHH
LCFGEATKFSQDLLDADKTYEATMRLGVRTTTGDAEGDVLDTRDVACDEATVGAALARFV
HHHCCHHHHHHHHHCCCHHHHHHHHCCEEECCCCCCCCEECCCCCCCCHHHHHHHHHHHH
GDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRTVTIRSLALLSCALPDVTFRVTCSK
HHHHCCCCHHHHHHHCCCHHHHHHHCCCCCHHCCCEEEEHHHHHHHHCCCCEEEEEEECC
GTYVRTLAEDIGEALGCGAHLTMLRRTGVGSLTLEHAVTLDALDAATQEERDARLAPVDA
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHHHH
LLSTFPLVRLDAALTKRFLHGQRLKLAELAARPEADEGERVRVYDADDRLLGVARASEGV
HHHHCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEECCCCEEEEHHCCCCC
LAPERLVVTGA
CCCCCEEEECC
>Mature Secondary Structure 
TTVSPRPRMARRALDGVLLLDKPVGLSSNDALMRAKRLYLAKKAGHTGTLDPLASGLLP
CCCCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCHH
LCFGEATKFSQDLLDADKTYEATMRLGVRTTTGDAEGDVLDTRDVACDEATVGAALARFV
HHHCCHHHHHHHHHCCCHHHHHHHHCCEEECCCCCCCCEECCCCCCCCHHHHHHHHHHHH
GDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRTVTIRSLALLSCALPDVTFRVTCSK
HHHHCCCCHHHHHHHCCCHHHHHHHCCCCCHHCCCEEEEHHHHHHHHCCCCEEEEEEECC
GTYVRTLAEDIGEALGCGAHLTMLRRTGVGSLTLEHAVTLDALDAATQEERDARLAPVDA
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHHHH
LLSTFPLVRLDAALTKRFLHGQRLKLAELAARPEADEGERVRVYDADDRLLGVARASEGV
HHHHCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEECCCCEEEEHHCCCCC
LAPERLVVTGA
CCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA