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The map label for this gene is metF [H]

Identifier: 83721420

GI number: 83721420

Start: 3607457

End: 3608287

Strand: Reverse

Name: metF [H]

Synonym: BTH_I3163

Alternate gene names: 83721420

Gene position: 3608287-3607457 (Counterclockwise)

Preceding gene: 83718543

Following gene: 83721023

Centisome position: 94.73

GC content: 67.27

Gene sequence:

>831_bases
ATGAACCCGATCGAACTTTCCTTTGAATTCTTTCCGCCGAAGACGGCGGAAGGCGTCGACAAGCTGCGCGCGACGCGCGC
GCAGCTCGCGCCGCTCAAGCCGAAATTCGTGTCCGTCACGTTCGGCGCGGGCGGCTCGACGCAGCAAGGCACGCTCGACA
CCGTGCTCGACATGCAGAAGGCGGGGCTCGAAGCCGCGCCGCACTTGTCGTGCATCGGCTCGTCGAAGGACAGCCTGCGC
GCGATTCTCGACCAGTACCGCTCGCACGGCATCCGCCACATCGTCGCGTTGCGCGGCGATCTGCCGTCCGGCATGGGCGA
GGTGGGCGAGCTGCGCTACGCGTCGGAGCTTGTCAGCTTCATCCGCGCCGAGCATGGCGACTGGTTCCGGATCGAAGTGG
CCGCGTATCCCGAGTATCACCCGCAGGCGCGATCGCCGAAGGCCGATCTCGAGAACTTCGCGCGCAAGGTGAAGGCGGGC
GCGAACGCCGCGATCACGCAGTATTTCTACAACGCGGACGCGTACTTCCGCTTCGTCGACGATGCGGCGAAGCTCGGCGT
CGACGTGCCGGTCGTGCCGGGGATCATGCCGATCACGAACTTCTCGCAGCTGATGCGCTTCTCCGAGATGTGCGGCGCGG
AAGTGCCGCGCTGGATCGCGCGCCGGCTCGAGAGCTTCGGCGACGACAAGGACTCGATCCGCGCGTTCGGCGCGGATGTC
GTCACGGGTCTTTGCCGGCGGCTCATCGACGCGGGCGTGCCCGGCCTGCACTTCTACACGCTCAACGGCGCGGCGGCGAC
GAAGGCCGTCTGCGAGCGGCTGGGGCTCTGA

Upstream 100 bases:

>100_bases
CCGCGTTCTTCGGTTCGATCCTGTACAGCATCGTGTCGTGGCTGCTGTCCGCCCTGATCTTCGGTCAGCGCAACATCGGC
TGACGGGTGATACCTGAATC

Downstream 100 bases:

>100_bases
CGCCGGCCGGCGCGGCGAGAGCGCCGCGCATCGGCTGCGATGACGAGGCGAAACCCTTGGGTCTCGCCTCTTTTTTTAGG
TTCATCGCGGAATCCCGGGG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MNPIELSFEFFPPKTAEGVDKLRATRAQLAPLKPKFVSVTFGAGGSTQQGTLDTVLDMQKAGLEAAPHLSCIGSSKDSLR
AILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEHGDWFRIEVAAYPEYHPQARSPKADLENFARKVKAG
ANAAITQYFYNADAYFRFVDDAAKLGVDVPVVPGIMPITNFSQLMRFSEMCGAEVPRWIARRLESFGDDKDSIRAFGADV
VTGLCRRLIDAGVPGLHFYTLNGAAATKAVCERLGL

Sequences:

>Translated_276_residues
MNPIELSFEFFPPKTAEGVDKLRATRAQLAPLKPKFVSVTFGAGGSTQQGTLDTVLDMQKAGLEAAPHLSCIGSSKDSLR
AILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEHGDWFRIEVAAYPEYHPQARSPKADLENFARKVKAG
ANAAITQYFYNADAYFRFVDDAAKLGVDVPVVPGIMPITNFSQLMRFSEMCGAEVPRWIARRLESFGDDKDSIRAFGADV
VTGLCRRLIDAGVPGLHFYTLNGAAATKAVCERLGL
>Mature_276_residues
MNPIELSFEFFPPKTAEGVDKLRATRAQLAPLKPKFVSVTFGAGGSTQQGTLDTVLDMQKAGLEAAPHLSCIGSSKDSLR
AILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEHGDWFRIEVAAYPEYHPQARSPKADLENFARKVKAG
ANAAITQYFYNADAYFRFVDDAAKLGVDVPVVPGIMPITNFSQLMRFSEMCGAEVPRWIARRLESFGDDKDSIRAFGADV
VTGLCRRLIDAGVPGLHFYTLNGAAATKAVCERLGL

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=280, Percent_Identity=39.2857142857143, Blast_Score=192, Evalue=2e-49,
Organism=Escherichia coli, GI1790377, Length=274, Percent_Identity=46.3503649635037, Blast_Score=252, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI25147436, Length=282, Percent_Identity=34.0425531914894, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI25147441, Length=282, Percent_Identity=34.0425531914894, Blast_Score=178, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321313, Length=281, Percent_Identity=35.2313167259786, Blast_Score=171, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6325234, Length=286, Percent_Identity=32.8671328671329, Blast_Score=155, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24662658, Length=263, Percent_Identity=26.9961977186312, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30157; Mature: 30157

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIELSFEFFPPKTAEGVDKLRATRAQLAPLKPKFVSVTFGAGGSTQQGTLDTVLDMQK
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
AGLEAAPHLSCIGSSKDSLRAILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSF
HCCCCCCCHHCCCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCCHHHHHHHHHHHHHHH
IRAEHGDWFRIEVAAYPEYHPQARSPKADLENFARKVKAGANAAITQYFYNADAYFRFVD
HHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
DAAKLGVDVPVVPGIMPITNFSQLMRFSEMCGAEVPRWIARRLESFGDDKDSIRAFGADV
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
VTGLCRRLIDAGVPGLHFYTLNGAAATKAVCERLGL
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure
MNPIELSFEFFPPKTAEGVDKLRATRAQLAPLKPKFVSVTFGAGGSTQQGTLDTVLDMQK
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
AGLEAAPHLSCIGSSKDSLRAILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSF
HCCCCCCCHHCCCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCCHHHHHHHHHHHHHHH
IRAEHGDWFRIEVAAYPEYHPQARSPKADLENFARKVKAGANAAITQYFYNADAYFRFVD
HHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
DAAKLGVDVPVVPGIMPITNFSQLMRFSEMCGAEVPRWIARRLESFGDDKDSIRAFGADV
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
VTGLCRRLIDAGVPGLHFYTLNGAAATKAVCERLGL
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6356036; 8346018; 9278503; 10201405 [H]