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The map label for this gene is purB [H]

Identifier: 83721185

GI number: 83721185

Start: 1368222

End: 1369673

Strand: Reverse

Name: purB [H]

Synonym: BTH_I1221

Alternate gene names: 83721185

Gene position: 1369673-1368222 (Counterclockwise)

Preceding gene: 83720264

Following gene: 83719424

Centisome position: 35.96

GC content: 65.7

Gene sequence:

>1452_bases
ATGGTAAAATTCGCGGTTCTCCCCTTCACGTTCGAACCGCGGCCGCGAGCCTCAAGCCCGATCATGTCCGACACCCGTCC
CGATACCCTTTTCGCCCTCACCGCGCTTTCGCCGATCGACGGCCGCTACGCCAGCAAGACCGAAGCGCTGCGCGACTGGC
TCTCCGAAGCCGCCTTCATGCGCCACCGCGTCACCGTCGAGGTGCACTGGCTGATCGCGCTGTCGCGCGCCGGCTTCGCC
GAAGTGCCGCGCTTCTCCGAAGCCGCCGAACAGTTCCTGCTGCAGCTCGTCGAGCGCTTCACCGCGCACGACGCCGCACG
CATCAAGGAAATCGAGCGCGTGACGAACCACGACGTGAAAGCCGTCGAGTACTGGCTGAAGGAATCCGTCAAGGGCCAGA
CCGAACTCGAGCGCGCGAGCGAATTCATCCACTTCGCGTGCACGTCGGAAGACATCAACAACACGTCGCACGGAATGATG
CTTGCCGGCGCGCGCCAGCACGTGATCCTGCCCGCGCTGCGCACGGTGCACGGCCGCCTCGTCGCGCTCGCGCACGCGCA
CGCCGGGCAACCGATGCTCTCGCGCACGCACGGCCAACCGGCCAGCCCGACGACGCTCGGCAAGGAAATCGCGAACGTCG
CGGCGCGCCTTGCGCGCGCGATCGAGCGCATCGAGAAGGTCGAGATCCTCGGCAAGATGAACGGCGCGGTCGGCAACTTC
AACGCTCACCTGTCCGCCTATCCGGAATTCGACTGGGAAGCGTTCTCGCGCGACGTGATCGAGAACCGCCTGAAGCTCGC
GTTCAACCCGTACACGATCCAGATCGAACCGCACGACTACATGGCCGAGCTGTTCGACGCCGTGGCGCGCGCGAACACGA
TCCTGCTCGACCTCGACCGCGACGTGTGGGGCTACATCTCGGTCGGCTACTTCAAGCAGAAGACGAAGGCGGGCGAAATC
GGCTCGTCGACGATGCCGCACAAGGTCAACCCGATCGACTTCGAAAACTCGGAAGGCAACCTCGGCCTCGCGAACGCGAC
GCTGCGCCACCTCGCCGACAAGCTGCCGGTGTCGCGCTGGCAGCGCGATCTCACCGATTCGACGGTGCTGCGCAACATGG
GCGTCGCGCTCGGCTACTCGCTGCTCGCGTACGACTCGCTGATCCGCGGCCTCGACAAGCTCGAGGTGAACCCGCAGCGC
CTGAACGAAGATCTCGACAACTGCTGGGAAGTGCTCGCCGAGCCGGTGCAGACGGTGATGCGCCGCTACGGCATCGAGAA
CCCGTACGAGCAGTTGAAGGAACTGACGCGCGGCAAGGGCATCACGCGCGAGGCGCTGCAGCAGTTCATCGGCACGCTCG
CGATTCCCGAGGACGCGAAGGCGCGCCTGCTCGCGATGACGCCCGCGTCGTACATCGGCAAGGCGGCCGAGCTCGCGAAA
CGCATCGCGTAA

Upstream 100 bases:

>100_bases
AGCCGGCCTCGGGCACCGGGATGCCGATCGGCGGCGCGGCGCGCGTCGGCGACTGAGATTTTCCGGCAACGGCGCGCGGC
GGCCGGGCGGCCGCGCGCCG

Downstream 100 bases:

>100_bases
CGCGCGTCGTCGACACGATGAAGAAAGGCGCCCTGCAGTTCGCAGGGCGCCTTTCTTTTTGCCGCCACAGCGCGGCGATC
GGCGGCGGCCGCGTCGCGGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MVKFAVLPFTFEPRPRASSPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFA
EVPRFSEAAEQFLLQLVERFTAHDAARIKEIERVTNHDVKAVEYWLKESVKGQTELERASEFIHFACTSEDINNTSHGMM
LAGARQHVILPALRTVHGRLVALAHAHAGQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVEILGKMNGAVGNF
NAHLSAYPEFDWEAFSRDVIENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYISVGYFKQKTKAGEI
GSSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNMGVALGYSLLAYDSLIRGLDKLEVNPQR
LNEDLDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQQFIGTLAIPEDAKARLLAMTPASYIGKAAELAK
RIA

Sequences:

>Translated_483_residues
MVKFAVLPFTFEPRPRASSPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFA
EVPRFSEAAEQFLLQLVERFTAHDAARIKEIERVTNHDVKAVEYWLKESVKGQTELERASEFIHFACTSEDINNTSHGMM
LAGARQHVILPALRTVHGRLVALAHAHAGQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVEILGKMNGAVGNF
NAHLSAYPEFDWEAFSRDVIENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYISVGYFKQKTKAGEI
GSSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNMGVALGYSLLAYDSLIRGLDKLEVNPQR
LNEDLDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQQFIGTLAIPEDAKARLLAMTPASYIGKAAELAK
RIA
>Mature_483_residues
MVKFAVLPFTFEPRPRASSPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFA
EVPRFSEAAEQFLLQLVERFTAHDAARIKEIERVTNHDVKAVEYWLKESVKGQTELERASEFIHFACTSEDINNTSHGMM
LAGARQHVILPALRTVHGRLVALAHAHAGQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVEILGKMNGAVGNF
NAHLSAYPEFDWEAFSRDVIENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYISVGYFKQKTKAGEI
GSSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNMGVALGYSLLAYDSLIRGLDKLEVNPQR
LNEDLDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQQFIGTLAIPEDAKARLLAMTPASYIGKAAELAK
RIA

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=454, Percent_Identity=62.9955947136564, Blast_Score=585, Evalue=1e-168,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 54094; Mature: 54094

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKFAVLPFTFEPRPRASSPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFM
CCEEEEECEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
RHRVTVEVHWLIALSRAGFAEVPRFSEAAEQFLLQLVERFTAHDAARIKEIERVTNHDVK
HHHEEEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AVEYWLKESVKGQTELERASEFIHFACTSEDINNTSHGMMLAGARQHVILPALRTVHGRL
HHHHHHHHHCCCHHHHHHHHHHEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
VALAHAHAGQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVEILGKMNGAVGNF
HHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NAHLSAYPEFDWEAFSRDVIENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDR
CCCEECCCCCCHHHHHHHHHHHHHHEEECCEEEEECCHHHHHHHHHHHHHCCEEEEEECC
DVWGYISVGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRW
CHHHHEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
QRDLTDSTVLRNMGVALGYSLLAYDSLIRGLDKLEVNPQRLNEDLDNCWEVLAEPVQTVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RRYGIENPYEQLKELTRGKGITREALQQFIGTLAIPEDAKARLLAMTPASYIGKAAELAK
HHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHH
RIA
HCC
>Mature Secondary Structure
MVKFAVLPFTFEPRPRASSPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFM
CCEEEEECEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
RHRVTVEVHWLIALSRAGFAEVPRFSEAAEQFLLQLVERFTAHDAARIKEIERVTNHDVK
HHHEEEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AVEYWLKESVKGQTELERASEFIHFACTSEDINNTSHGMMLAGARQHVILPALRTVHGRL
HHHHHHHHHCCCHHHHHHHHHHEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
VALAHAHAGQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVEILGKMNGAVGNF
HHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NAHLSAYPEFDWEAFSRDVIENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDR
CCCEECCCCCCHHHHHHHHHHHHHHEEECCEEEEECCHHHHHHHHHHHHHCCEEEEEECC
DVWGYISVGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRW
CHHHHEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
QRDLTDSTVLRNMGVALGYSLLAYDSLIRGLDKLEVNPQRLNEDLDNCWEVLAEPVQTVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RRYGIENPYEQLKELTRGKGITREALQQFIGTLAIPEDAKARLLAMTPASYIGKAAELAK
HHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHH
RIA
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]