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The map label for this gene is nasA [H]

Identifier: 83720264

GI number: 83720264

Start: 1369718

End: 1371538

Strand: Reverse

Name: nasA [H]

Synonym: BTH_I1222

Alternate gene names: 83720264

Gene position: 1371538-1369718 (Counterclockwise)

Preceding gene: 83719954

Following gene: 83721185

Centisome position: 36.01

GC content: 71.17

Gene sequence:

>1821_bases
ATGGACGCATGGGCGCGCACGGGCCGAAGCCCCCCCTGTGGCGCACGCGTCGGCAAACGGCCCGGGGCCGCGGGCGCCTC
GCCATACCCTACGCTTGATTCTTCACACATTTGCGCACCGCACCAATGCCGCGCGCACGCACAGGCGCAGTGCCGCGCGG
CGCTCGCGCGCGCCTGCCGCCGGTGCGCGCGAGCGCCGCGCCGCCGCGCGCCCGCCCGTCACCCGCCGCTTTCATCGCTT
GCGCCGCCTTTCCCGCCTGCCTTGCCCGGCGTGCGTTCGCGTCGTTGGGAGCAAATACATGCACGCCATTGGCATGAAGC
TTGCGGAACAGCGCACCGGCCGTCCCGCGCAGCGCGGAGCGGCTCGCCTCTTTCCCACCATCCGCCGTCGACCGGCATAC
GTGAGGCTTCACCGATGGATACTCTGCGCACCTCCCTTCGAAGCGGCAACTGGCGCTCGCTTCTCGCGTGCTTCCTCTAC
TTCGATACGGGCTTCACCGTCTGGGTGCTGTACGGCCCGCTCGCGCCGTTCATCAGCAAGGACATCACGATGACGGCCGC
GCAACAGGGCTTCCTCGTCGCGGTGCCCGTGCTCGCGGCGGCCATCCTGCGCGTGACGCTCGGCAACCTCTATCAGTCCG
CCGACGGCCGGCGCATCGCGCTGATGGGCGTGCTGCTGTCCGCGCTGCCCGCCGCCGTGCTGCCGTTCGTGCCCGGCACG
CCGTCATACACCCTGCTCCTCGTACTCGGCGTGTTCCTCGGGATCGGCGGCGCGAGCTTCGCAGTCGCGCTGCCGATGGC
GGGCAGCAGCTATCCGCCGAAGGTGCAGGGCCTCGTGCTCGGCCTCGCCGCCGCGGGCAACATCGGCGCAGTGCTCGACG
GCTTCATGTTCCCGCACATCGCGGCCGCGCTCGGCTGGAAGTTCTCGACGGCCGCCGCGCTGCCGCTCCTCGCGATCGCG
GGCTTCGCGCTCTTCGCGTGGGCCGACGACCGCGGCGAGAAATCGGGCAGCGCGAAGCGTGCGTTCGGCGCGTTCGCGCT
CACACTTGCCGGTCTCGTCGCGCTCGTGCTCGCCGTGCATGCGGGCGTGTTCGGCACGGGCAAGACAGGCGTGCTGCTGC
TGCCCGTCATGGGCGCGCTCCTCGCGATCGCCGTGCTCCCCGGCCGCTATCGCGCAGTGCTCGCGGAGCGCGACACGTGG
GTCATCATGCTGATCTACAGCATCACGTTTGGCGGCTTCGTCGGCATGTCGTCTTACGTGACGCTGCTTCTGACGACGCT
CTATCAGATGCCGAAGATCGAAGCGGGCCTCTTCATGTCGCTGCTCGCGTTCCTCGGCGCGATCGTGCGCCCGTTCGGCG
GCCACCTCGCCGACCGCATCACCGGCGTGCGCGCGCTGATGGTGATCCTCGCCGCGATCGCCGCGGCAGACTTCGCGTTC
GCGGCCGTGATGCCGCCGCTCGCGGGCGGCATCGCGCTCCTCGTGTGCCTGTACGTCGCGTTCGGCCTCGGCAACGGCTC
GACGTTCCAGCTCGTCCCGCACCGCTGGAAAGGCAAGACGGGCCTGCTGTCCGGCATCGTCGGCGCGGCGGGCGGCATCG
GCGGCTTCTATCTGCCCGTCGTGATGGGCATCGCGAAGGAAAGCACGGGCAGCTACCAGCTCGGCTTCGCGACGTTCGGC
GCGCTCGCGACCTGCGCGCTCGTCGCGCTCGCGATGCTGCGCGCACAGTGGCTGCGCTGGGCCGCGCCCGAGCTCGCCGC
GGCCGAGCCGGCCTCGGGCACCGGGATGCCGATCGGCGGCGCGGCGCGCGTCGGCGACTGA

Upstream 100 bases:

>100_bases
GTGCCTTTGCTGCGCAAAGCGAGTGCGAGGGAAGAATCACGCATTTTAACGGTCGGTGGCGCAGATCGCCCGGCGCGTCG
GAGCGTCGGAGCGTCGGCGC

Downstream 100 bases:

>100_bases
GATTTTCCGGCAACGGCGCGCGGCGGCCGGGCGGCCGCGCGCCGATGGTAAAATTCGCGGTTCTCCCCTTCACGTTCGAA
CCGCGGCCGCGAGCCTCAAG

Product: nitrate transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MDAWARTGRSPPCGARVGKRPGAAGASPYPTLDSSHICAPHQCRAHAQAQCRAALARACRRCARAPRRRAPARHPPLSSL
APPFPPALPGVRSRRWEQIHARHWHEACGTAHRPSRAARSGSPLSHHPPSTGIREASPMDTLRTSLRSGNWRSLLACFLY
FDTGFTVWVLYGPLAPFISKDITMTAAQQGFLVAVPVLAAAILRVTLGNLYQSADGRRIALMGVLLSALPAAVLPFVPGT
PSYTLLLVLGVFLGIGGASFAVALPMAGSSYPPKVQGLVLGLAAAGNIGAVLDGFMFPHIAAALGWKFSTAAALPLLAIA
GFALFAWADDRGEKSGSAKRAFGAFALTLAGLVALVLAVHAGVFGTGKTGVLLLPVMGALLAIAVLPGRYRAVLAERDTW
VIMLIYSITFGGFVGMSSYVTLLLTTLYQMPKIEAGLFMSLLAFLGAIVRPFGGHLADRITGVRALMVILAAIAAADFAF
AAVMPPLAGGIALLVCLYVAFGLGNGSTFQLVPHRWKGKTGLLSGIVGAAGGIGGFYLPVVMGIAKESTGSYQLGFATFG
ALATCALVALAMLRAQWLRWAAPELAAAEPASGTGMPIGGAARVGD

Sequences:

>Translated_606_residues
MDAWARTGRSPPCGARVGKRPGAAGASPYPTLDSSHICAPHQCRAHAQAQCRAALARACRRCARAPRRRAPARHPPLSSL
APPFPPALPGVRSRRWEQIHARHWHEACGTAHRPSRAARSGSPLSHHPPSTGIREASPMDTLRTSLRSGNWRSLLACFLY
FDTGFTVWVLYGPLAPFISKDITMTAAQQGFLVAVPVLAAAILRVTLGNLYQSADGRRIALMGVLLSALPAAVLPFVPGT
PSYTLLLVLGVFLGIGGASFAVALPMAGSSYPPKVQGLVLGLAAAGNIGAVLDGFMFPHIAAALGWKFSTAAALPLLAIA
GFALFAWADDRGEKSGSAKRAFGAFALTLAGLVALVLAVHAGVFGTGKTGVLLLPVMGALLAIAVLPGRYRAVLAERDTW
VIMLIYSITFGGFVGMSSYVTLLLTTLYQMPKIEAGLFMSLLAFLGAIVRPFGGHLADRITGVRALMVILAAIAAADFAF
AAVMPPLAGGIALLVCLYVAFGLGNGSTFQLVPHRWKGKTGLLSGIVGAAGGIGGFYLPVVMGIAKESTGSYQLGFATFG
ALATCALVALAMLRAQWLRWAAPELAAAEPASGTGMPIGGAARVGD
>Mature_606_residues
MDAWARTGRSPPCGARVGKRPGAAGASPYPTLDSSHICAPHQCRAHAQAQCRAALARACRRCARAPRRRAPARHPPLSSL
APPFPPALPGVRSRRWEQIHARHWHEACGTAHRPSRAARSGSPLSHHPPSTGIREASPMDTLRTSLRSGNWRSLLACFLY
FDTGFTVWVLYGPLAPFISKDITMTAAQQGFLVAVPVLAAAILRVTLGNLYQSADGRRIALMGVLLSALPAAVLPFVPGT
PSYTLLLVLGVFLGIGGASFAVALPMAGSSYPPKVQGLVLGLAAAGNIGAVLDGFMFPHIAAALGWKFSTAAALPLLAIA
GFALFAWADDRGEKSGSAKRAFGAFALTLAGLVALVLAVHAGVFGTGKTGVLLLPVMGALLAIAVLPGRYRAVLAERDTW
VIMLIYSITFGGFVGMSSYVTLLLTTLYQMPKIEAGLFMSLLAFLGAIVRPFGGHLADRITGVRALMVILAAIAAADFAF
AAVMPPLAGGIALLVCLYVAFGLGNGSTFQLVPHRWKGKTGLLSGIVGAAGGIGGFYLPVVMGIAKESTGSYQLGFATFG
ALATCALVALAMLRAQWLRWAAPELAAAEPASGTGMPIGGAARVGD

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=187, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 62993; Mature: 62993

Theoretical pI: Translated: 10.88; Mature: 10.88

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAWARTGRSPPCGARVGKRPGAAGASPYPTLDSSHICAPHQCRAHAQAQCRAALARACR
CCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RCARAPRRRAPARHPPLSSLAPPFPPALPGVRSRRWEQIHARHWHEACGTAHRPSRAARS
HHHHCHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
GSPLSHHPPSTGIREASPMDTLRTSLRSGNWRSLLACFLYFDTGFTVWVLYGPLAPFISK
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHC
DITMTAAQQGFLVAVPVLAAAILRVTLGNLYQSADGRRIALMGVLLSALPAAVLPFVPGT
CCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC
PSYTLLLVLGVFLGIGGASFAVALPMAGSSYPPKVQGLVLGLAAAGNIGAVLDGFMFPHI
CHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
AAALGWKFSTAAALPLLAIAGFALFAWADDRGEKSGSAKRAFGAFALTLAGLVALVLAVH
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AGVFGTGKTGVLLLPVMGALLAIAVLPGRYRAVLAERDTWVIMLIYSITFGGFVGMSSYV
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHH
TLLLTTLYQMPKIEAGLFMSLLAFLGAIVRPFGGHLADRITGVRALMVILAAIAAADFAF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVMPPLAGGIALLVCLYVAFGLGNGSTFQLVPHRWKGKTGLLSGIVGAAGGIGGFYLPV
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHH
VMGIAKESTGSYQLGFATFGALATCALVALAMLRAQWLRWAAPELAAAEPASGTGMPIGG
HHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCC
AARVGD
CCCCCC
>Mature Secondary Structure
MDAWARTGRSPPCGARVGKRPGAAGASPYPTLDSSHICAPHQCRAHAQAQCRAALARACR
CCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RCARAPRRRAPARHPPLSSLAPPFPPALPGVRSRRWEQIHARHWHEACGTAHRPSRAARS
HHHHCHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
GSPLSHHPPSTGIREASPMDTLRTSLRSGNWRSLLACFLYFDTGFTVWVLYGPLAPFISK
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHC
DITMTAAQQGFLVAVPVLAAAILRVTLGNLYQSADGRRIALMGVLLSALPAAVLPFVPGT
CCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC
PSYTLLLVLGVFLGIGGASFAVALPMAGSSYPPKVQGLVLGLAAAGNIGAVLDGFMFPHI
CHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
AAALGWKFSTAAALPLLAIAGFALFAWADDRGEKSGSAKRAFGAFALTLAGLVALVLAVH
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AGVFGTGKTGVLLLPVMGALLAIAVLPGRYRAVLAERDTWVIMLIYSITFGGFVGMSSYV
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHH
TLLLTTLYQMPKIEAGLFMSLLAFLGAIVRPFGGHLADRITGVRALMVILAAIAAADFAF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVMPPLAGGIALLVCLYVAFGLGNGSTFQLVPHRWKGKTGLLSGIVGAAGGIGGFYLPV
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHH
VMGIAKESTGSYQLGFATFGALATCALVALAMLRAQWLRWAAPELAAAEPASGTGMPIGG
HHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCC
AARVGD
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]