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The map label for this gene is proP [H]

Identifier: 83721118

GI number: 83721118

Start: 736464

End: 738122

Strand: Reverse

Name: proP [H]

Synonym: BTH_I0640

Alternate gene names: 83721118

Gene position: 738122-736464 (Counterclockwise)

Preceding gene: 83719031

Following gene: 83720150

Centisome position: 19.38

GC content: 64.62

Gene sequence:

>1659_bases
ATGGCAACGGTTGGCGGACAAATTGCGCACGCGCCGATGACGGGCGACGAAAAGCGCGTGATCTTCGCATCGTCGCTCGG
CACCGTGTTCGAGTGGTACGACTTCTATCTGGCCGGCTCGCTGGCGGCCTACATCAGCAAGAGCTTCTTTTCCGGCGTCA
ATCCGACCGCGGCGTTCATCTTCACGCTGCTCGGCTTCGCGGCGGGCTTCGCGGTCCGGCCCTTCGGCGCGATCGTGTTC
GGCCGCCTCGGCGACCTCGTCGGCCGCAAGCACACATTCCTCATCACGATCGTGATCATGGGGATCTCGACCTTCGTCGT
GGGCTTCCTGCCGGGCTACGCATCGATCGGCATCGCCGCGCCCGTGATCTTCATCGCGATGCGGCTGATGCAGGGCCTCG
CGCTCGGCGGCGAGTACGGCGGCGCGGCGACCTACGTCGCCGAGCACGCGCCCGCGCATCGCCGCGGCTTCTATACGTCG
TGGATCCAGACGACCGCGACGCTCGGCCTGTTCCTGTCGCTGCTCGTGATCCTCGGCGTGCGCACCGCGATCGGCGAGGA
AGCGTTCGGCAACTGGGGCTGGCGCGTGCCGTTCGTCGCGTCGATCCTGCTGCTCGCCGTGTCCGTGTGGATCCGCCTGC
AACTGAACGAGTCGCCGGTGTTCCTGCGGATCAAGGCCGAGGGCAAGACGTCGAAGGCGCCGCTCACGGAAGCGTTCGGC
CAATGGAAGAACCTGAAGATCGTGATCCTCGCGCTGATCGGGCTGACGGCCGGCCAGGCGGTTGTCTGGTACACGGGCCA
GTTCTATGCGCTCTTCTTCCTCACGCAAACGCTGAAGGTCGACGGAGGAAGCGCGAACATCCTGATCGCGCTCGCCCTTT
TGATCGGCACGCCGTTCTTCGTGTTCTTCGGCTCGCTGTCGGACAAGATCGGCCGCAAGCCGATCATCCTCGCCGGCTGC
CTGATCGCCGCGCTCACCTACTTCCCGCTCTTCAAGGCGCTCACGCACTTCGCGAATCCGGCGCTCGAGGCGGCGACGCA
AAAGGCGCCGATCACCGTGGTCGCCAATCCCGGCGAATGCTCGTTCCAGTTCAACCCGGTGGGCACGGCGAAATTCACGT
CGTCCTGCGACATCGCGAAGAGCGCGCTCGCGAAATCGGGCCTCAATTACGAGAACGTCGCCGCGCCGGCGGGCACGCTC
GCGCAGATCAAGATCGGCGACACGGTGATCGACACGTATGACGGCAAGGCGGCCGACGCGAAGGACAAGGCGAAGGCATT
CGACAAGACGCTCGCGTCGACGCTGAAGACGGCCGGCTACCCGACCAAGGCCGATCCGGCGCAGCTCAACTGGCCGATGA
CGACGGCGATCCTCGCGATTCTCGTGATCTACGTGACGATGGTGTACGGGCCGATCGCGGCGATGCTGGTCGAGATGTTC
CCGACGCGGATACGCTACACGTCGATGTCGCTGCCGTATCACATCGGCAACGGCTGGTTCGGCGGCTTCCTGCCGGCAAC
CGCGTTCGCGATCGTCGCGGCCAACGGCGACATCTACTCGGGGCTCTGGTATCCGATCGGCGTCGCGCTCATCACGTTCG
TGATCGGGCTGCTGTTCGTGAAGGAAACCAAGGATTCGAATATTTACGCGCAGGATTGA

Upstream 100 bases:

>100_bases
CGCGGCGAGTCAGTGCGCCGTAAGTTTGCCCTCCAATAATCGACTCGGAGCCGCCCTCCCGAGAGGGCGCGCGACCTCTA
TATCAAAATTGGAGACATTC

Downstream 100 bases:

>100_bases
GGACGGGCGGGGCGCGGCGGGCTGCGGCGCGAGTGATGCCTCGGAGGGAGGGACGTCTAGCCCGTCGACCGTTGGGCGAC
GGCCGGCGCCGGTTGGCGTC

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MATVGGQIAHAPMTGDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVF
GRLGDLVGRKHTFLITIVIMGISTFVVGFLPGYASIGIAAPVIFIAMRLMQGLALGGEYGGAATYVAEHAPAHRRGFYTS
WIQTTATLGLFLSLLVILGVRTAIGEEAFGNWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFG
QWKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGGSANILIALALLIGTPFFVFFGSLSDKIGRKPIILAGC
LIAALTYFPLFKALTHFANPALEAATQKAPITVVANPGECSFQFNPVGTAKFTSSCDIAKSALAKSGLNYENVAAPAGTL
AQIKIGDTVIDTYDGKAADAKDKAKAFDKTLASTLKTAGYPTKADPAQLNWPMTTAILAILVIYVTMVYGPIAAMLVEMF
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAANGDIYSGLWYPIGVALITFVIGLLFVKETKDSNIYAQD

Sequences:

>Translated_552_residues
MATVGGQIAHAPMTGDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVF
GRLGDLVGRKHTFLITIVIMGISTFVVGFLPGYASIGIAAPVIFIAMRLMQGLALGGEYGGAATYVAEHAPAHRRGFYTS
WIQTTATLGLFLSLLVILGVRTAIGEEAFGNWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFG
QWKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGGSANILIALALLIGTPFFVFFGSLSDKIGRKPIILAGC
LIAALTYFPLFKALTHFANPALEAATQKAPITVVANPGECSFQFNPVGTAKFTSSCDIAKSALAKSGLNYENVAAPAGTL
AQIKIGDTVIDTYDGKAADAKDKAKAFDKTLASTLKTAGYPTKADPAQLNWPMTTAILAILVIYVTMVYGPIAAMLVEMF
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAANGDIYSGLWYPIGVALITFVIGLLFVKETKDSNIYAQD
>Mature_551_residues
ATVGGQIAHAPMTGDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVFG
RLGDLVGRKHTFLITIVIMGISTFVVGFLPGYASIGIAAPVIFIAMRLMQGLALGGEYGGAATYVAEHAPAHRRGFYTSW
IQTTATLGLFLSLLVILGVRTAIGEEAFGNWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFGQ
WKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGGSANILIALALLIGTPFFVFFGSLSDKIGRKPIILAGCL
IAALTYFPLFKALTHFANPALEAATQKAPITVVANPGECSFQFNPVGTAKFTSSCDIAKSALAKSGLNYENVAAPAGTLA
QIKIGDTVIDTYDGKAADAKDKAKAFDKTLASTLKTAGYPTKADPAQLNWPMTTAILAILVIYVTMVYGPIAAMLVEMFP
TRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAANGDIYSGLWYPIGVALITFVIGLLFVKETKDSNIYAQD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=328, Percent_Identity=35.6707317073171, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788292, Length=326, Percent_Identity=33.7423312883436, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1788942, Length=333, Percent_Identity=35.1351351351351, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1789941, Length=346, Percent_Identity=35.8381502890173, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI87082231, Length=223, Percent_Identity=29.1479820627803, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87082404, Length=323, Percent_Identity=24.4582043343653, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 58999; Mature: 58867

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVGGQIAHAPMTGDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFI
CCCCCCCEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FTLLGFAAGFAVRPFGAIVFGRLGDLVGRKHTFLITIVIMGISTFVVGFLPGYASIGIAA
HHHHHHHHCCHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PVIFIAMRLMQGLALGGEYGGAATYVAEHAPAHRRGFYTSWIQTTATLGLFLSLLVILGV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RTAIGEEAFGNWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFG
HHHHCHHHHCCCCCCHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCCCHHHHHC
QWKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGGSANILIALALLIGTPFF
CCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCHHH
VFFGSLSDKIGRKPIILAGCLIAALTYFPLFKALTHFANPALEAATQKAPITVVANPGEC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCC
SFQFNPVGTAKFTSSCDIAKSALAKSGLNYENVAAPAGTLAQIKIGDTVIDTYDGKAADA
EEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCCC
KDKAKAFDKTLASTLKTAGYPTKADPAQLNWPMTTAILAILVIYVTMVYGPIAAMLVEMF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAANGDIYSGLWYPIGVALITFVIGLLFV
HHHEEEEEECCCEEECCCCCCHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
KETKDSNIYAQD
HCCCCCCCEECC
>Mature Secondary Structure 
ATVGGQIAHAPMTGDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFI
CCCCCCEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FTLLGFAAGFAVRPFGAIVFGRLGDLVGRKHTFLITIVIMGISTFVVGFLPGYASIGIAA
HHHHHHHHCCHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PVIFIAMRLMQGLALGGEYGGAATYVAEHAPAHRRGFYTSWIQTTATLGLFLSLLVILGV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RTAIGEEAFGNWGWRVPFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFG
HHHHCHHHHCCCCCCHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCCCHHHHHC
QWKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGGSANILIALALLIGTPFF
CCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCHHH
VFFGSLSDKIGRKPIILAGCLIAALTYFPLFKALTHFANPALEAATQKAPITVVANPGEC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCC
SFQFNPVGTAKFTSSCDIAKSALAKSGLNYENVAAPAGTLAQIKIGDTVIDTYDGKAADA
EEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCCC
KDKAKAFDKTLASTLKTAGYPTKADPAQLNWPMTTAILAILVIYVTMVYGPIAAMLVEMF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAANGDIYSGLWYPIGVALITFVIGLLFV
HHHEEEEEECCCEEECCCCCCHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
KETKDSNIYAQD
HCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]