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The map label for this gene is sdaA-1 [H]

Identifier: 83720891

GI number: 83720891

Start: 3707506

End: 3708894

Strand: Reverse

Name: sdaA-1 [H]

Synonym: BTH_I3251

Alternate gene names: 83720891

Gene position: 3708894-3707506 (Counterclockwise)

Preceding gene: 83719132

Following gene: 83720725

Centisome position: 97.37

GC content: 69.33

Gene sequence:

>1389_bases
ATGGCAGTCAGCGTGTTCGATCTTTTCAAAATCGGTATCGGCCCGTCGAGCTCGCACACGGTCGGGCCGATGCGCGCCGC
ACTGATGTTCGCGCAAGGGCTCGAGCGCGACGCGCTGCTCGCCGCGACCGCGAGCGTGAAGGTCGAGCTGTACGGCTCGC
TCGGCGCGACCGGCAAGGGCCACGGCACGGACCGCGGCGTGATGCTCGGCCTGATGGGCGACGCGCCCGACACCGTCGAT
CCGTCGACGATCGCCGCGCGGCTCGATGCGGTGCGCGACTCGAGAACGCTCGCGCTGCTCGGCACGCACCCGGTGCCGTT
CGTGCCGAAGGAGCACATCGCGTTCTACCGGCAGGCGCTGCCCGAGCATCCGAACGCGATGAAGCTGCGCGCGACCGACG
CGACGGGCGCGATGCTGCGCGAGGCGACGTATCTGTCGGTGGGCGGCGGCTTCGTCGTCACCGCGGGCGCGCCGAACACG
AAGGTGCTCGCCGCCGTCGAGCAAATGCCGCATCCGTTCCGCACGGGCGCCGAGCTGCTCGCGCTCACCACATCGACCGG
CAAGTCGATCGCGCAGCTGATGTGGGACAACGAGCGCGCGTGGCACACCGAGGCGGAAACGCGCACGGGCCTGCTGAAGA
TCTGGGACGTGATGCAATCGTGCGTCGCGCGCGGCTGCGGGATCGGCAACCCCGACGCGGACGGCACCCTGCCCGGGCCG
TTCCAGGTCAAGCGCCGCGCGCCGCAGCTCTATCGCGCGCTGACGGGCAATCCGGAACGCGCGCTGCAAGACCCGCTGTC
GATGGTCGACTGGATCAACCTGTATGCGATCGCCGTCAACGAGGAAAACGCGGCGGGCGGGCGCGTCGTCACCGCGCCGA
CCAACGGCGCGGCGGGCATCATCCCGGCCGTGCTCCATTACTACACGCGCTTCACGCCGGGCGCGAACGAGCAAGGCGTG
ATCGACTTCCTGATGACCGCGGCGGCGATCGGCATCCTGTACAAGCTCAACGCGTCGATCTCGGGCGCAGAAGTCGGCTG
CCAGGGCGAAGTCGGCGTCGCGTGCTCGATGGCGGCGGGCGCGCTCGCGGCGGTGCTCGGCGGCACGCCGCAGCAGGTCG
AGAACGCGGCCGAGATCGGGATGGAACACAACCTCGGCCTCACCTGCGATCCGGTCGGCGGGATGGTGCAGATTCCGTGC
ATCGAGCGCAACGCGATGGCGTCGGTGAAGGCCGTCAACGCCGCGCGGATGGCGCTGCGCGGCGACGGCAGCCACTACGT
GTCGCTCGACTCCGTGATCAAGACGATGCGCGAAACCGGCGCGGACATGAAGACGAAGTACAAGGAAACCTCGCGCGGCG
GGCTGGCGGTGAATATCGTCGAGTGCTGA

Upstream 100 bases:

>100_bases
CGCCCAACAACTGTCGCCGACGCCGCCGGCTTGGCTGGCCGCGTGCCTGCCGCTGCCGGCGCGGTGATCCCCCAATACAT
CCCGTCACTCAGGAAACGAA

Downstream 100 bases:

>100_bases
CGCGCGGGCGCGCCGCGCATCGCGTCGGCGCGGCGCGCGAACATCGCTGCCCTGCAGCACGCGGGCGCGCCTTCCGTCGC
CCCTTCAACCGGCGTAAGCT

Product: L-serine ammonia-lyase

Products: NA

Alternate protein names: SDH; L-serine deaminase; L-SD [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MAVSVFDLFKIGIGPSSSHTVGPMRAALMFAQGLERDALLAATASVKVELYGSLGATGKGHGTDRGVMLGLMGDAPDTVD
PSTIAARLDAVRDSRTLALLGTHPVPFVPKEHIAFYRQALPEHPNAMKLRATDATGAMLREATYLSVGGGFVVTAGAPNT
KVLAAVEQMPHPFRTGAELLALTTSTGKSIAQLMWDNERAWHTEAETRTGLLKIWDVMQSCVARGCGIGNPDADGTLPGP
FQVKRRAPQLYRALTGNPERALQDPLSMVDWINLYAIAVNEENAAGGRVVTAPTNGAAGIIPAVLHYYTRFTPGANEQGV
IDFLMTAAAIGILYKLNASISGAEVGCQGEVGVACSMAAGALAAVLGGTPQQVENAAEIGMEHNLGLTCDPVGGMVQIPC
IERNAMASVKAVNAARMALRGDGSHYVSLDSVIKTMRETGADMKTKYKETSRGGLAVNIVEC

Sequences:

>Translated_462_residues
MAVSVFDLFKIGIGPSSSHTVGPMRAALMFAQGLERDALLAATASVKVELYGSLGATGKGHGTDRGVMLGLMGDAPDTVD
PSTIAARLDAVRDSRTLALLGTHPVPFVPKEHIAFYRQALPEHPNAMKLRATDATGAMLREATYLSVGGGFVVTAGAPNT
KVLAAVEQMPHPFRTGAELLALTTSTGKSIAQLMWDNERAWHTEAETRTGLLKIWDVMQSCVARGCGIGNPDADGTLPGP
FQVKRRAPQLYRALTGNPERALQDPLSMVDWINLYAIAVNEENAAGGRVVTAPTNGAAGIIPAVLHYYTRFTPGANEQGV
IDFLMTAAAIGILYKLNASISGAEVGCQGEVGVACSMAAGALAAVLGGTPQQVENAAEIGMEHNLGLTCDPVGGMVQIPC
IERNAMASVKAVNAARMALRGDGSHYVSLDSVIKTMRETGADMKTKYKETSRGGLAVNIVEC
>Mature_461_residues
AVSVFDLFKIGIGPSSSHTVGPMRAALMFAQGLERDALLAATASVKVELYGSLGATGKGHGTDRGVMLGLMGDAPDTVDP
STIAARLDAVRDSRTLALLGTHPVPFVPKEHIAFYRQALPEHPNAMKLRATDATGAMLREATYLSVGGGFVVTAGAPNTK
VLAAVEQMPHPFRTGAELLALTTSTGKSIAQLMWDNERAWHTEAETRTGLLKIWDVMQSCVARGCGIGNPDADGTLPGPF
QVKRRAPQLYRALTGNPERALQDPLSMVDWINLYAIAVNEENAAGGRVVTAPTNGAAGIIPAVLHYYTRFTPGANEQGVI
DFLMTAAAIGILYKLNASISGAEVGCQGEVGVACSMAAGALAAVLGGTPQQVENAAEIGMEHNLGLTCDPVGGMVQIPCI
ERNAMASVKAVNAARMALRGDGSHYVSLDSVIKTMRETGADMKTKYKETSRGGLAVNIVEC

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI1788116, Length=461, Percent_Identity=52.060737527115, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI48994925, Length=460, Percent_Identity=53.4782608695652, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1789161, Length=461, Percent_Identity=52.9284164859002, Blast_Score=454, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 48426; Mature: 48295

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSVFDLFKIGIGPSSSHTVGPMRAALMFAQGLERDALLAATASVKVELYGSLGATGKG
CEEEHEEHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECCCCCCCC
HGTDRGVMLGLMGDAPDTVDPSTIAARLDAVRDSRTLALLGTHPVPFVPKEHIAFYRQAL
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHC
PEHPNAMKLRATDATGAMLREATYLSVGGGFVVTAGAPNTKVLAAVEQMPHPFRTGAELL
CCCCCCEEEEECCCHHHHHHHCEEEEECCCEEEECCCCCCHHHHHHHHCCCCCCCCCEEE
ALTTSTGKSIAQLMWDNERAWHTEAETRTGLLKIWDVMQSCVARGCGIGNPDADGTLPGP
EEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
FQVKRRAPQLYRALTGNPERALQDPLSMVDWINLYAIAVNEENAAGGRVVTAPTNGAAGI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCEEEECCCCCCCHH
IPAVLHYYTRFTPGANEQGVIDFLMTAAAIGILYKLNASISGAEVGCQGEVGVACSMAAG
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHH
ALAAVLGGTPQQVENAAEIGMEHNLGLTCDPVGGMVQIPCIERNAMASVKAVNAARMALR
HHHHHHCCCHHHHHHHHHCCCCCCCCEEECCCCCEEEEEEECCCHHHHHHHHHHHHHEEE
GDGSHYVSLDSVIKTMRETGADMKTKYKETSRGGLAVNIVEC
CCCCCEEEHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEEC
>Mature Secondary Structure 
AVSVFDLFKIGIGPSSSHTVGPMRAALMFAQGLERDALLAATASVKVELYGSLGATGKG
EEEHEEHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECCCCCCCC
HGTDRGVMLGLMGDAPDTVDPSTIAARLDAVRDSRTLALLGTHPVPFVPKEHIAFYRQAL
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHC
PEHPNAMKLRATDATGAMLREATYLSVGGGFVVTAGAPNTKVLAAVEQMPHPFRTGAELL
CCCCCCEEEEECCCHHHHHHHCEEEEECCCEEEECCCCCCHHHHHHHHCCCCCCCCCEEE
ALTTSTGKSIAQLMWDNERAWHTEAETRTGLLKIWDVMQSCVARGCGIGNPDADGTLPGP
EEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
FQVKRRAPQLYRALTGNPERALQDPLSMVDWINLYAIAVNEENAAGGRVVTAPTNGAAGI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCEEEECCCCCCCHH
IPAVLHYYTRFTPGANEQGVIDFLMTAAAIGILYKLNASISGAEVGCQGEVGVACSMAAG
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHH
ALAAVLGGTPQQVENAAEIGMEHNLGLTCDPVGGMVQIPCIERNAMASVKAVNAARMALR
HHHHHHCCCHHHHHHHHHCCCCCCCCEEECCCCCEEEEEEECCCHHHHHHHHHHHHHEEE
GDGSHYVSLDSVIKTMRETGADMKTKYKETSRGGLAVNIVEC
CCCCCEEEHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]