Click here to switch to the map view.

The map label for this gene is pckG [H]

Identifier: 83720809

GI number: 83720809

Start: 3223376

End: 3225241

Strand: Reverse

Name: pckG [H]

Synonym: BTH_I2808

Alternate gene names: 83720809

Gene position: 3225241-3223376 (Counterclockwise)

Preceding gene: 83721469

Following gene: 83720341

Centisome position: 84.67

GC content: 68.7

Gene sequence:

>1866_bases
ATGACCCGAAGCAACGTGGTCGCGGCGACGCGCACCATTCCGATCGACGTTCCCGAATACGTGAAGCACCGCGGCCTGAT
CGACTGGGTCGCGCGTATCGCGGAACTGACCGAGCCGGATCGCGTCGTCTGGTGCGACGGTTCGCAAGAGGAGTACGACC
GCCTTTGCGACGCGATGGTCGAGCAGCGCACGATGGTGCGCCTGAATCCGGCGAAGCGGCCGAACTCGTTTCTCGCGCTG
TCCGATCCTTCGGACGTCGCGCGCGTCGAAGACCGCACGTTCATCTGCAGCGAGCATCGCGACGACGCGGGCCCGACCAA
TCACTGGATCGCGCCCGCCGAAATGCGCGCGACGCTCAACGGTCTGTTTCGCGGCGCGATGCGCGGCCGCACGCTGTACG
TCGTGCCGTTCTCGATGGGGCCGCTCGGCTCGCCCATCGCGCATATCGGCGTCGAGCTGTCGGACAGCCCGTACGTCGTC
GCCAACATGCGGATCATGACGCGCATGGGGCGCGCCGTGCTCGACGCGCTCGGCGAGCGCGGCGATTACGTGCCGTGCGT
GCACAGCGTCGGGCGTCCGCTCGCCGCGGGCGAGCGGGACGTTCCGTGGCCGTGCAATCCGACCAAGTACATCGTGCATT
TCCCCGAGTCGCGCGAGATCTGGAGCTTCGGCTCGGGCTACGGCGGCAACGCGCTGCTCGGCAAGAAGTGCTTCGCGCTG
CGGATCGCGTCGACGATGGGGCGCGACGAGGGCTGGCTCGCCGAGCACATGTTGATTCTCGGCGTGACGTCGCCGCAAGG
GCGCAAGTATCACATCGCCGCGGCGTTTCCGTCCGCGTGCGGCAAGACCAACTTCGCGATGCTGATTCCGCCGAAGGGCT
TCGACGGCTGGCGCGTGACGACGATCGGCGACGACATCGCATGGCTCAAGCCGGGCCGCGACGGGCGCCTGTACGCGATC
AACCCGGAGGCGGGCTACTTCGGCGTCGCGCCGGGTACGGGCGAGAAGACGAACCCGAACGCGCTTGCGACGCTCACGGA
AAACGTGATCTTCACGAACGTCGCGTTGACGGAAGACGGCGACGTCTGGTGGGAAGGGCTCACCGATACGCCGCCCGCGC
GGCTCACCGACTGGCAGGGCAACGCATGGACGCCCGAGATCGGCCGCGAGACGGGCCGCAAGGCCGCGCATCCGAATTCG
CGCTTCACGGCGCCCGCGTCGCAGTGCCCGTCGATCGACGGCGACTGGGAGAACCCGGCCGGCGTGCCGATCGATGCGTT
CATCTTCGGCGGCCGCCGCTCGACGACGGTGCCGCTCGTCACCGAGGCGCGCGACTGGGTCGAAGGCGTCTACATGGCGG
CGACGATGGGCTCCGAGACGACGGCGGCGGCGGCGGGGCAGCAGGGCATCGTGCGGCGCGATCCGTTCGCGATGCTGCCG
TTCTGCGGCTACAACATGAGCGACTACTTCGCGCACTGGCTCGCGCTCGGCGAGAAGCTCGCGGCGGCGGGCGCGACGCT
GCCGAAGATCTACTGCGTGAACTGGTTCCGCAAGGATGCGGACGGCCGCTTCGCGTGGCCGGGCTTCGGCGAGAACATGC
GCGTGCTGAAGTGGATGCTCGACCGGATCGACGGCCACGGCGAGGGCGTCGAGCATGCGTTCGGCGTGACGCCGCGCTAC
GAGGATCTGCATTGGTCGGGGCTCGCGTTCTCGCCCGAGCAGTTCGCGCAGGTCACGTCGATGAATCCCGCAGAATGGCG
CGCCGAGCTCGCGCTGCACGCGGAGCTGTTCGACAGGCTGAGCGCGCGGCTGCCGGGCGCGCTCGCCGAAACGAAGGCGA
AGATCGAAGCCAGGCTCGGCGACTGA

Upstream 100 bases:

>100_bases
CCGCCCCCAGCCATCGCGTAGCGCCGCCATGCCCGTCGCCGCGCGCGTCGTCGCGCGGCGCACGCACGGATTTCTGCTTC
TACAACACGGAGACACTCTC

Downstream 100 bases:

>100_bases
GCGACGCGGCAACCCGGCAACTCGGCGGCGACGCGGCCGAGCCGGCGCGCGCCGTGTCCGGCGCGCGCCGGGACGCACGC
GATGCGTGCCGCTCGCGCCG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MTRSNVVAATRTIPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQEEYDRLCDAMVEQRTMVRLNPAKRPNSFLAL
SDPSDVARVEDRTFICSEHRDDAGPTNHWIAPAEMRATLNGLFRGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVV
ANMRIMTRMGRAVLDALGERGDYVPCVHSVGRPLAAGERDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFAL
RIASTMGRDEGWLAEHMLILGVTSPQGRKYHIAAAFPSACGKTNFAMLIPPKGFDGWRVTTIGDDIAWLKPGRDGRLYAI
NPEAGYFGVAPGTGEKTNPNALATLTENVIFTNVALTEDGDVWWEGLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNS
RFTAPASQCPSIDGDWENPAGVPIDAFIFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLP
FCGYNMSDYFAHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWMLDRIDGHGEGVEHAFGVTPRY
EDLHWSGLAFSPEQFAQVTSMNPAEWRAELALHAELFDRLSARLPGALAETKAKIEARLGD

Sequences:

>Translated_621_residues
MTRSNVVAATRTIPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQEEYDRLCDAMVEQRTMVRLNPAKRPNSFLAL
SDPSDVARVEDRTFICSEHRDDAGPTNHWIAPAEMRATLNGLFRGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVV
ANMRIMTRMGRAVLDALGERGDYVPCVHSVGRPLAAGERDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFAL
RIASTMGRDEGWLAEHMLILGVTSPQGRKYHIAAAFPSACGKTNFAMLIPPKGFDGWRVTTIGDDIAWLKPGRDGRLYAI
NPEAGYFGVAPGTGEKTNPNALATLTENVIFTNVALTEDGDVWWEGLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNS
RFTAPASQCPSIDGDWENPAGVPIDAFIFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLP
FCGYNMSDYFAHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWMLDRIDGHGEGVEHAFGVTPRY
EDLHWSGLAFSPEQFAQVTSMNPAEWRAELALHAELFDRLSARLPGALAETKAKIEARLGD
>Mature_620_residues
TRSNVVAATRTIPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQEEYDRLCDAMVEQRTMVRLNPAKRPNSFLALS
DPSDVARVEDRTFICSEHRDDAGPTNHWIAPAEMRATLNGLFRGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVVA
NMRIMTRMGRAVLDALGERGDYVPCVHSVGRPLAAGERDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFALR
IASTMGRDEGWLAEHMLILGVTSPQGRKYHIAAAFPSACGKTNFAMLIPPKGFDGWRVTTIGDDIAWLKPGRDGRLYAIN
PEAGYFGVAPGTGEKTNPNALATLTENVIFTNVALTEDGDVWWEGLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNSR
FTAPASQCPSIDGDWENPAGVPIDAFIFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLPF
CGYNMSDYFAHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWMLDRIDGHGEGVEHAFGVTPRYE
DLHWSGLAFSPEQFAQVTSMNPAEWRAELALHAELFDRLSARLPGALAETKAKIEARLGD

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=606, Percent_Identity=50.990099009901, Blast_Score=593, Evalue=1e-169,
Organism=Homo sapiens, GI66346721, Length=609, Percent_Identity=50.0821018062397, Blast_Score=570, Evalue=1e-162,
Organism=Homo sapiens, GI66346723, Length=387, Percent_Identity=53.4883720930233, Blast_Score=394, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71989645, Length=555, Percent_Identity=52.2522522522522, Blast_Score=561, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71989658, Length=555, Percent_Identity=52.2522522522522, Blast_Score=560, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71989653, Length=546, Percent_Identity=52.3809523809524, Blast_Score=553, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI17555492, Length=617, Percent_Identity=48.9465153970827, Blast_Score=550, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI32564411, Length=558, Percent_Identity=52.3297491039427, Blast_Score=546, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI32564407, Length=529, Percent_Identity=51.4177693761815, Blast_Score=502, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI71989649, Length=482, Percent_Identity=53.5269709543568, Blast_Score=499, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI32564409, Length=375, Percent_Identity=48.2666666666667, Blast_Score=316, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI17540956, Length=539, Percent_Identity=24.30426716141, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24655085, Length=600, Percent_Identity=50.1666666666667, Blast_Score=583, Evalue=1e-166,
Organism=Drosophila melanogaster, GI24655082, Length=608, Percent_Identity=48.1907894736842, Blast_Score=566, Evalue=1e-161,
Organism=Drosophila melanogaster, GI161077226, Length=577, Percent_Identity=48.8734835355286, Blast_Score=543, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24655088, Length=512, Percent_Identity=51.5625, Blast_Score=518, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 68343; Mature: 68212

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSNVVAATRTIPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQEEYDRLCDAMV
CCCCCEEEEEEEECCCCHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
EQRTMVRLNPAKRPNSFLALSDPSDVARVEDRTFICSEHRDDAGPTNHWIAPAEMRATLN
HCCCEEEECCCCCCCCEEEECCCCHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
GLFRGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVVANMRIMTRMGRAVLDALGER
HHHHHHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEHHHHHHHHHHHHHHHHHCC
GDYVPCVHSVGRPLAAGERDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFAL
CCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCHHCCHHHHH
RIASTMGRDEGWLAEHMLILGVTSPQGRKYHIAAAFPSACGKTNFAMLIPPKGFDGWRVT
HHHHHCCCCCCHHHEEEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCEEEE
TIGDDIAWLKPGRDGRLYAINPEAGYFGVAPGTGEKTNPNALATLTENVIFTNVALTEDG
EECCCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCHHEEEECCEEEEEEEEECCC
DVWWEGLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNSRFTAPASQCPSIDGDWENPA
CEEECCCCCCCCCCEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCC
GVPIDAFIFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLP
CCCEEEEEECCCCCCEEEEEHHHHHHHHHEEEEEECCCCCHHHHCCCCCCEECCCCEECC
FCGYNMSDYFAHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWML
CCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCEECCCCCCCHHHHHHHH
DRIDGHGEGVEHAFGVTPRYEDLHWSGLAFSPEQFAQVTSMNPAEWRAELALHAELFDRL
HHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
SARLPGALAETKAKIEARLGD
HHHCCCHHHHHHHHHEEECCC
>Mature Secondary Structure 
TRSNVVAATRTIPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQEEYDRLCDAMV
CCCCEEEEEEEECCCCHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
EQRTMVRLNPAKRPNSFLALSDPSDVARVEDRTFICSEHRDDAGPTNHWIAPAEMRATLN
HCCCEEEECCCCCCCCEEEECCCCHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
GLFRGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVVANMRIMTRMGRAVLDALGER
HHHHHHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEHHHHHHHHHHHHHHHHHCC
GDYVPCVHSVGRPLAAGERDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFAL
CCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCHHCCHHHHH
RIASTMGRDEGWLAEHMLILGVTSPQGRKYHIAAAFPSACGKTNFAMLIPPKGFDGWRVT
HHHHHCCCCCCHHHEEEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCEEEE
TIGDDIAWLKPGRDGRLYAINPEAGYFGVAPGTGEKTNPNALATLTENVIFTNVALTEDG
EECCCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCHHEEEECCEEEEEEEEECCC
DVWWEGLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNSRFTAPASQCPSIDGDWENPA
CEEECCCCCCCCCCEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCC
GVPIDAFIFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLP
CCCEEEEEECCCCCCEEEEEHHHHHHHHHEEEEEECCCCCHHHHCCCCCCEECCCCEECC
FCGYNMSDYFAHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWML
CCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCEECCCCCCCHHHHHHHH
DRIDGHGEGVEHAFGVTPRYEDLHWSGLAFSPEQFAQVTSMNPAEWRAELALHAELFDRL
HHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
SARLPGALAETKAKIEARLGD
HHHCCCHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA