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The map label for this gene is rpfG [H]

Identifier: 83720806

GI number: 83720806

Start: 702036

End: 703490

Strand: Reverse

Name: rpfG [H]

Synonym: BTH_I0615

Alternate gene names: 83720806

Gene position: 703490-702036 (Counterclockwise)

Preceding gene: 83721541

Following gene: 83718882

Centisome position: 18.47

GC content: 68.66

Gene sequence:

>1455_bases
GTGAACGGACCCGAGGAATCGACAACGCCCATCGCGCCGGATGCGCCGCCCCCCCGCGCGGCCGATGATGCGCGCTGCGA
CGGCGCCGCGCCGGCCGTCGCGGCGGGCGCCGACGGTGACGCGCCCGCCGTGCCCGTGATCCTGATCGTCGACGACGAGC
CGAGCATCCTGTCCGCGCTCAAGCGCCTGCTGCGCACGGCGCGCTACCAGGTCGTGACGGCCGAGAGCGGCGCCGCGGCG
CTCGACGTGCTCGCCGCCGGCGAAGCCGACCTCATCATCTCCGACATGCGGATGCCCGGCATGACGGGGGCCGAATTCCT
CGCGCGCGCGCAGGCGCTCCATCCGGACACGATGCGCATCCTGCTGACGGGCTATTCCGAGATCGATGCGGTCGTGAGCG
CGATCAACGAAGGCGGCGTGTATCGCTACCTGAACAAGCCGTGGGACGATCACGACCTGCTGCTGACGGTCAAGCAGGCG
CTCGAGCAACGGCGTCTGCGCCAGCAGACCGCGCGCCTCTTCGCGCTCACGCAGCAGCAGAACGCGGAGCTCCTCGCATT
CAGCACCGAACTCGAAGCGCAAGTGCATGCGCGCACCGAGGAGATCCGGCAGACCGTGATGTTCCTCGAGGACGCGCAGC
GCGACCTGAAGCGCAATTTCATGACGATGATCCAGGTCGGCGCGAACATGATCGAGCTGCGCTGCGGCGTGATGGGCGGC
GAATCGCGGCGCATCGGCGAACTCGCGAAGCGGCTCGCGCTCGCGTCCGACATGAGCGAGCTGCAGGCGCAGGATCTGCA
TTTCGCCGGGCTTCTGCACGGGATCGGCAAGCTGTCGCTGCCGGACGAGCTGCTGCGCAAATCGATCGACAGGATGAGCG
CCGAAGAGAGCCGGCAGTTCCATCAGCATCCGCTGCGCGCGCAGATGGTGCTCACGCCGGTCGCGCAACTCAATCACGTC
TCGCACATCATTCGCCATCAATACGAGCGCTTCAACGGGCGCGGCACGCCCGACGGCCTCGCGGGGGACGATATCCCGCT
CGGCTCGCGCATCCTTGCGATCGCGCGCGACTACGAGGGATTGCAGCGCGGCGGCGTCGTCAATCAGCGTATGCAGCCCG
AGCAGGCGATCGCGCTCATCAAGTCGCAGGCGGGCCTGCGCTACGACCCGGAGATCGTCGATCGCTTCGCCGCGCTCGTG
AAGGACCTGGCGACGCTCGGCTGCAACACGCCGTATGCGCGGATCACGGGCGGCCAGTTGCTCGAAGGCATGCGGCTCGC
CGACGATCTGCGCACGAGCCGCGGCGTGCTGCTGATGACCAAGGGCAGCGTCGTGTCCGCCCACCAGGTCGCGCTCGTGC
GGCGCTACGAGGCGCAGGAGAGCACGCCGTTCACGATCCTGATCCAGACGATGCCGCTCGCCGAGGCGGCGGCGGCAGCC
GGACACGAATGCTGA

Upstream 100 bases:

>100_bases
CTAGGTAAATACGCTTACGCCCCATTTTTTTTGCTCAAGCGCGCGCGCCCGCGCGCCGATATCGCAGCTAGCCGATGACA
AAAACTGAGACGGAGGCTTC

Downstream 100 bases:

>100_bases
CGCGCGCGCCGGCAAGCGCCAACGGCCCATGCATGGAACCTTCGATCTCCCGCTCGTCACGCTGTCGCTCGCGATCGCGA
CGCTCGCGTCGTATACGGCG

Product: response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MNGPEESTTPIAPDAPPPRAADDARCDGAAPAVAAGADGDAPAVPVILIVDDEPSILSALKRLLRTARYQVVTAESGAAA
LDVLAAGEADLIISDMRMPGMTGAEFLARAQALHPDTMRILLTGYSEIDAVVSAINEGGVYRYLNKPWDDHDLLLTVKQA
LEQRRLRQQTARLFALTQQQNAELLAFSTELEAQVHARTEEIRQTVMFLEDAQRDLKRNFMTMIQVGANMIELRCGVMGG
ESRRIGELAKRLALASDMSELQAQDLHFAGLLHGIGKLSLPDELLRKSIDRMSAEESRQFHQHPLRAQMVLTPVAQLNHV
SHIIRHQYERFNGRGTPDGLAGDDIPLGSRILAIARDYEGLQRGGVVNQRMQPEQAIALIKSQAGLRYDPEIVDRFAALV
KDLATLGCNTPYARITGGQLLEGMRLADDLRTSRGVLLMTKGSVVSAHQVALVRRYEAQESTPFTILIQTMPLAEAAAAA
GHEC

Sequences:

>Translated_484_residues
MNGPEESTTPIAPDAPPPRAADDARCDGAAPAVAAGADGDAPAVPVILIVDDEPSILSALKRLLRTARYQVVTAESGAAA
LDVLAAGEADLIISDMRMPGMTGAEFLARAQALHPDTMRILLTGYSEIDAVVSAINEGGVYRYLNKPWDDHDLLLTVKQA
LEQRRLRQQTARLFALTQQQNAELLAFSTELEAQVHARTEEIRQTVMFLEDAQRDLKRNFMTMIQVGANMIELRCGVMGG
ESRRIGELAKRLALASDMSELQAQDLHFAGLLHGIGKLSLPDELLRKSIDRMSAEESRQFHQHPLRAQMVLTPVAQLNHV
SHIIRHQYERFNGRGTPDGLAGDDIPLGSRILAIARDYEGLQRGGVVNQRMQPEQAIALIKSQAGLRYDPEIVDRFAALV
KDLATLGCNTPYARITGGQLLEGMRLADDLRTSRGVLLMTKGSVVSAHQVALVRRYEAQESTPFTILIQTMPLAEAAAAA
GHEC
>Mature_484_residues
MNGPEESTTPIAPDAPPPRAADDARCDGAAPAVAAGADGDAPAVPVILIVDDEPSILSALKRLLRTARYQVVTAESGAAA
LDVLAAGEADLIISDMRMPGMTGAEFLARAQALHPDTMRILLTGYSEIDAVVSAINEGGVYRYLNKPWDDHDLLLTVKQA
LEQRRLRQQTARLFALTQQQNAELLAFSTELEAQVHARTEEIRQTVMFLEDAQRDLKRNFMTMIQVGANMIELRCGVMGG
ESRRIGELAKRLALASDMSELQAQDLHFAGLLHGIGKLSLPDELLRKSIDRMSAEESRQFHQHPLRAQMVLTPVAQLNHV
SHIIRHQYERFNGRGTPDGLAGDDIPLGSRILAIARDYEGLQRGGVVNQRMQPEQAIALIKSQAGLRYDPEIVDRFAALV
KDLATLGCNTPYARITGGQLLEGMRLADDLRTSRGVLLMTKGSVVSAHQVALVRRYEAQESTPFTILIQTMPLAEAAAAA
GHEC

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=124, Percent_Identity=37.0967741935484, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788905, Length=117, Percent_Identity=41.8803418803419, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790437, Length=115, Percent_Identity=37.3913043478261, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788550, Length=135, Percent_Identity=32.5925925925926, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 52765; Mature: 52765

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGPEESTTPIAPDAPPPRAADDARCDGAAPAVAAGADGDAPAVPVILIVDDEPSILSAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCCHHHHHH
KRLLRTARYQVVTAESGAAALDVLAAGEADLIISDMRMPGMTGAEFLARAQALHPDTMRI
HHHHHHHHEEEEEECCCCHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCEEE
LLTGYSEIDAVVSAINEGGVYRYLNKPWDDHDLLLTVKQALEQRRLRQQTARLFALTQQQ
EEECHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NAELLAFSTELEAQVHARTEEIRQTVMFLEDAQRDLKRNFMTMIQVGANMIELRCGVMGG
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHEECCCCC
ESRRIGELAKRLALASDMSELQAQDLHFAGLLHGIGKLSLPDELLRKSIDRMSAEESRQF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHH
HQHPLRAQMVLTPVAQLNHVSHIIRHQYERFNGRGTPDGLAGDDIPLGSRILAIARDYEG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LQRGGVVNQRMQPEQAIALIKSQAGLRYDPEIVDRFAALVKDLATLGCNTPYARITGGQL
HHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
LEGMRLADDLRTSRGVLLMTKGSVVSAHQVALVRRYEAQESTPFTILIQTMPLAEAAAAA
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHC
GHEC
CCCC
>Mature Secondary Structure
MNGPEESTTPIAPDAPPPRAADDARCDGAAPAVAAGADGDAPAVPVILIVDDEPSILSAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCCHHHHHH
KRLLRTARYQVVTAESGAAALDVLAAGEADLIISDMRMPGMTGAEFLARAQALHPDTMRI
HHHHHHHHEEEEEECCCCHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCEEE
LLTGYSEIDAVVSAINEGGVYRYLNKPWDDHDLLLTVKQALEQRRLRQQTARLFALTQQQ
EEECHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NAELLAFSTELEAQVHARTEEIRQTVMFLEDAQRDLKRNFMTMIQVGANMIELRCGVMGG
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHEECCCCC
ESRRIGELAKRLALASDMSELQAQDLHFAGLLHGIGKLSLPDELLRKSIDRMSAEESRQF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHH
HQHPLRAQMVLTPVAQLNHVSHIIRHQYERFNGRGTPDGLAGDDIPLGSRILAIARDYEG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LQRGGVVNQRMQPEQAIALIKSQAGLRYDPEIVDRFAALVKDLATLGCNTPYARITGGQL
HHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
LEGMRLADDLRTSRGVLLMTKGSVVSAHQVALVRRYEAQESTPFTILIQTMPLAEAAAAA
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHC
GHEC
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]