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The map label for this gene is gcvA [H]

Identifier: 83720760

GI number: 83720760

Start: 3110694

End: 3111599

Strand: Reverse

Name: gcvA [H]

Synonym: BTH_I2716

Alternate gene names: 83720760

Gene position: 3111599-3110694 (Counterclockwise)

Preceding gene: 83720284

Following gene: 83719417

Centisome position: 81.69

GC content: 56.62

Gene sequence:

>906_bases
ATGAAAATTCACCCGATTCCTCCCCTGCAATGCCTTGTGTTCTTTGATGCTGCAGCTCGGCACGGTAACTTCACGCGTGC
AGCCGAAGAACTGAACGTGACGCAAGGCGCGGTCAGCAAACAGGTTGTCAAGCTGGAAAGTTATCTTGGTATGCAGCTGT
TTGTGCGAGATGCAAAGGCGCTTCATTTGACACGTGCCGGGCAGACATATGCCGGTCGGATCCACGATATGCTCACTGGC
TGCGCGGAGGCCACCGCGCTTGTCATGAAGGAGCCTTCACCCCATAGCATCACGATCGCGTGTGCGTCGGGCACGGCCGG
CATGTTTTTGACACCCATCATCGCCGATTTCAGTCACGAGTATCCCAATGTCGCAATTCGGATCTTGGTTCGAGAAGGCG
TTTTCAATCTGAACCCCGCGGAATTCGACATTGGTGTGTATTACATTCGCGATGTCCCGCCTCCTGGCATGAGCGGAATC
CGTATCATCGAAGAGGAGGTGCAAGCCTATTGTGCGCCCAGCTACCTGGGACGCCGGCGCGTATCGCCGCACGAATTGAC
GAACGTGACATTACTCGTGGCAGAGGAGCAGCAGCGCCAATGGATGAGCTGGCGGGACTGGTTCCACCTATGCGATAGCG
AGATCGCATACAAGCCCTCGCGCACGATTACAGCCAATAGCTATCCGGTATTGCTGCAGCTTGCATTGCGAGGTCACGGT
GTCCTTCTCGGATGGAACCAAATGATCACGCCCTTGGTTGAACGCTCTGATTTGGTGCTTGCATCGGATGCGAGCGCGAG
CTTCGGCGGCGCGTATCAGCTCGTTTGGCCCGATGATCGGCGCGATACGGCGGCTGTGACGCATTTTCGGGAGTGGTTGC
TGAAGCGGGTGCCGTGGAAGGGGTGA

Upstream 100 bases:

>100_bases
TTCGATTGAATGACGCTTTCGATTAATGAAAAATGTGCCGCCAAGCGTTTGTATGCGCTTGTGATCCCCAGTCGGTGGAT
CAGAGCCATATGGAGCGAGT

Downstream 100 bases:

>100_bases
CCGGGTATTCCAGAAACTCAGTCTCGCCAGAAAAAAATTCAGAGTAAGCGGTCGAGAAAAAACCGTACCATTGGCTACGC
CGTTGATCGTTGCCAGTGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKIHPIPPLQCLVFFDAAARHGNFTRAAEELNVTQGAVSKQVVKLESYLGMQLFVRDAKALHLTRAGQTYAGRIHDMLTG
CAEATALVMKEPSPHSITIACASGTAGMFLTPIIADFSHEYPNVAIRILVREGVFNLNPAEFDIGVYYIRDVPPPGMSGI
RIIEEEVQAYCAPSYLGRRRVSPHELTNVTLLVAEEQQRQWMSWRDWFHLCDSEIAYKPSRTITANSYPVLLQLALRGHG
VLLGWNQMITPLVERSDLVLASDASASFGGAYQLVWPDDRRDTAAVTHFREWLLKRVPWKG

Sequences:

>Translated_301_residues
MKIHPIPPLQCLVFFDAAARHGNFTRAAEELNVTQGAVSKQVVKLESYLGMQLFVRDAKALHLTRAGQTYAGRIHDMLTG
CAEATALVMKEPSPHSITIACASGTAGMFLTPIIADFSHEYPNVAIRILVREGVFNLNPAEFDIGVYYIRDVPPPGMSGI
RIIEEEVQAYCAPSYLGRRRVSPHELTNVTLLVAEEQQRQWMSWRDWFHLCDSEIAYKPSRTITANSYPVLLQLALRGHG
VLLGWNQMITPLVERSDLVLASDASASFGGAYQLVWPDDRRDTAAVTHFREWLLKRVPWKG
>Mature_301_residues
MKIHPIPPLQCLVFFDAAARHGNFTRAAEELNVTQGAVSKQVVKLESYLGMQLFVRDAKALHLTRAGQTYAGRIHDMLTG
CAEATALVMKEPSPHSITIACASGTAGMFLTPIIADFSHEYPNVAIRILVREGVFNLNPAEFDIGVYYIRDVPPPGMSGI
RIIEEEVQAYCAPSYLGRRRVSPHELTNVTLLVAEEQQRQWMSWRDWFHLCDSEIAYKPSRTITANSYPVLLQLALRGHG
VLLGWNQMITPLVERSDLVLASDASASFGGAYQLVWPDDRRDTAAVTHFREWLLKRVPWKG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=31.0580204778157, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1788706, Length=177, Percent_Identity=29.9435028248588, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786448, Length=307, Percent_Identity=26.0586319218241, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=26.2798634812287, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=234, Percent_Identity=29.4871794871795, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787589, Length=303, Percent_Identity=24.7524752475248, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790262, Length=194, Percent_Identity=27.319587628866, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI2367136, Length=163, Percent_Identity=28.8343558282209, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI145693105, Length=151, Percent_Identity=27.8145695364238, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789440, Length=243, Percent_Identity=24.6913580246914, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI145693193, Length=304, Percent_Identity=23.6842105263158, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33688; Mature: 33688

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIHPIPPLQCLVFFDAAARHGNFTRAAEELNVTQGAVSKQVVKLESYLGMQLFVRDAKA
CCCCCCCCHHHHEEEHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHEECCHHH
LHLTRAGQTYAGRIHDMLTGCAEATALVMKEPSPHSITIACASGTAGMFLTPIIADFSHE
EEEEECCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCC
YPNVAIRILVREGVFNLNPAEFDIGVYYIRDVPPPGMSGIRIIEEEVQAYCAPSYLGRRR
CCCEEEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCHHHHHCCC
VSPHELTNVTLLVAEEQQRQWMSWRDWFHLCDSEIAYKPSRTITANSYPVLLQLALRGHG
CCCHHHCEEEEEEECHHHHHHHHHHHHHHHCCCHHEECCCCEEECCCCCEEEEEEECCCE
VLLGWNQMITPLVERSDLVLASDASASFGGAYQLVWPDDRRDTAAVTHFREWLLKRVPWK
EEEEHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
G
C
>Mature Secondary Structure
MKIHPIPPLQCLVFFDAAARHGNFTRAAEELNVTQGAVSKQVVKLESYLGMQLFVRDAKA
CCCCCCCCHHHHEEEHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHEECCHHH
LHLTRAGQTYAGRIHDMLTGCAEATALVMKEPSPHSITIACASGTAGMFLTPIIADFSHE
EEEEECCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCC
YPNVAIRILVREGVFNLNPAEFDIGVYYIRDVPPPGMSGIRIIEEEVQAYCAPSYLGRRR
CCCEEEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCHHHHHCCC
VSPHELTNVTLLVAEEQQRQWMSWRDWFHLCDSEIAYKPSRTITANSYPVLLQLALRGHG
CCCHHHCEEEEEEECHHHHHHHHHHHHHHHCCCHHEECCCCEEECCCCCEEEEEEECCCE
VLLGWNQMITPLVERSDLVLASDASASFGGAYQLVWPDDRRDTAAVTHFREWLLKRVPWK
EEEEHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]