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The map label for this gene is citH [H]

Identifier: 83719417

GI number: 83719417

Start: 3109275

End: 3110561

Strand: Reverse

Name: citH [H]

Synonym: BTH_I2715

Alternate gene names: 83719417

Gene position: 3110561-3109275 (Counterclockwise)

Preceding gene: 83720760

Following gene: 83718508

Centisome position: 81.66

GC content: 51.98

Gene sequence:

>1287_bases
ATGGAAACCGTCGCTGGCTTGCGCGAGGGTAAGCGGAGCTTCGCCGTACAGGAGAGGACAAAGGCAGTCGTCGCCGTGAT
CATAGGAAATGGATTCGAGTGGTTCGATTTTATTTCCTATGGCTTCTTTTCCGTCATCATTGCAAAAATATTCTTTCCAA
CTTCGGATGACAATCTGTCTCTGCTGCTGTCTGTCTCCACGATTGGTGTCGGATTTTTTATGCGGCCGGTGGGCGGTATT
GTCTTGGGAGGGATGGCGGATAAAATCGGGCGTCGTGCAACCTTGGTCATCACGATTACGTTGATGACCATCGGCACATC
CCTGATTGCTTTTGCACCAACCTATAAGGATGCCGGTATTTTCGCGCCATTGATGATCGTATGTGCGCGCCTGTTGCAAG
GGTTTTCCGCCGGAGGTGAAATGGGCGGCGCCACAGGATTTCTAAGAGACAATGTGCCCGCCGAACGGCTGGGCTATTAC
ACCAGTTGGATTCAGGCAAGCATCGGATTCGCGATTATTCTGGCATCGGTTCTTGCGGTTGTGCTTGTCAAGGTTCTGAG
CGCCGAGCAGGTGGAGTCATGGGGGTGGCGCATACCGTTCTTGATTGGCTTGTGTCTCGGGCCGCTCGGGATCTATATCC
GCAATCAAGTGCATGAACCCGCGGAGGAGAATGTTCAAATCCGAGAGCGGACGCCGGTGCTCGAGATTGTGCGTCGGTGG
AAGAGCGAAACGCTGATAGGATTTGGCCTTGTAATATTTTGGACCGTTTGCTCATATGTTCTGCTCTTCTATATCCCCAC
GTACGCGTCAAAGGTCTTGCATTTGCCGGCGTCGACAGGGTTTATTGCCGTTCTCGTTGGTGCGTCGATTGTTCTCTTTG
TGACGCCGGTTTTCGGTTATCTATCCGATCGCTACGGGCGGCGTCGATTTCTCATGGGGGCGCTTGCTGTGGCTGTCATG
GTTGCGTATCCCATGTTCAGACTCTTGAATGTGAGCCCTGGTTTGCATTCTCTGCTGCTATTTCAGGTTGTTTTCGGACT
TGTTATTGCATGCTATGAAGGGCCAATCCTCGCTGCATTGAGTGATGCGTTTCCGCGTAAAATAGTCTCCACGGGAATTT
CAATTTCGTACAATCTCGCCGTCATTACGTTCGGTGGATTTTCGGCGGCCATTCTTACTTGGGCGATTGCGGCAACACAA
AATAATCTTGCTCCCGCGTTTTATGTGATGGGCACGGCAGTCCTCAGTTTTTTTGCGGCGGCATGCTGGTCTCCGCGCAA
GGAGTGA

Upstream 100 bases:

>100_bases
AAAAATTCAGAGTAAGCGGTCGAGAAAAAACCGTACCATTGGCTACGCCGTTGATCGTTGCCAGTGGCGCCAGAACATCA
AAATCCGAGGGCACAAGAAT

Downstream 100 bases:

>100_bases
AGCGGTAATGGAGGCGGGTGTCTCGTTGGTGTCATGGTTGTATCGATGAGTTTATTTGATCTGATGAATTGAGGGGTGGT
GAAAGCTTGGTTTGGAATGC

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
METVAGLREGKRSFAVQERTKAVVAVIIGNGFEWFDFISYGFFSVIIAKIFFPTSDDNLSLLLSVSTIGVGFFMRPVGGI
VLGGMADKIGRRATLVITITLMTIGTSLIAFAPTYKDAGIFAPLMIVCARLLQGFSAGGEMGGATGFLRDNVPAERLGYY
TSWIQASIGFAIILASVLAVVLVKVLSAEQVESWGWRIPFLIGLCLGPLGIYIRNQVHEPAEENVQIRERTPVLEIVRRW
KSETLIGFGLVIFWTVCSYVLLFYIPTYASKVLHLPASTGFIAVLVGASIVLFVTPVFGYLSDRYGRRRFLMGALAVAVM
VAYPMFRLLNVSPGLHSLLLFQVVFGLVIACYEGPILAALSDAFPRKIVSTGISISYNLAVITFGGFSAAILTWAIAATQ
NNLAPAFYVMGTAVLSFFAAACWSPRKE

Sequences:

>Translated_428_residues
METVAGLREGKRSFAVQERTKAVVAVIIGNGFEWFDFISYGFFSVIIAKIFFPTSDDNLSLLLSVSTIGVGFFMRPVGGI
VLGGMADKIGRRATLVITITLMTIGTSLIAFAPTYKDAGIFAPLMIVCARLLQGFSAGGEMGGATGFLRDNVPAERLGYY
TSWIQASIGFAIILASVLAVVLVKVLSAEQVESWGWRIPFLIGLCLGPLGIYIRNQVHEPAEENVQIRERTPVLEIVRRW
KSETLIGFGLVIFWTVCSYVLLFYIPTYASKVLHLPASTGFIAVLVGASIVLFVTPVFGYLSDRYGRRRFLMGALAVAVM
VAYPMFRLLNVSPGLHSLLLFQVVFGLVIACYEGPILAALSDAFPRKIVSTGISISYNLAVITFGGFSAAILTWAIAATQ
NNLAPAFYVMGTAVLSFFAAACWSPRKE
>Mature_428_residues
METVAGLREGKRSFAVQERTKAVVAVIIGNGFEWFDFISYGFFSVIIAKIFFPTSDDNLSLLLSVSTIGVGFFMRPVGGI
VLGGMADKIGRRATLVITITLMTIGTSLIAFAPTYKDAGIFAPLMIVCARLLQGFSAGGEMGGATGFLRDNVPAERLGYY
TSWIQASIGFAIILASVLAVVLVKVLSAEQVESWGWRIPFLIGLCLGPLGIYIRNQVHEPAEENVQIRERTPVLEIVRRW
KSETLIGFGLVIFWTVCSYVLLFYIPTYASKVLHLPASTGFIAVLVGASIVLFVTPVFGYLSDRYGRRRFLMGALAVAVM
VAYPMFRLLNVSPGLHSLLLFQVVFGLVIACYEGPILAALSDAFPRKIVSTGISISYNLAVITFGGFSAAILTWAIAATQ
NNLAPAFYVMGTAVLSFFAAACWSPRKE

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=412, Percent_Identity=34.4660194174757, Blast_Score=241, Evalue=9e-65,
Organism=Escherichia coli, GI1788292, Length=409, Percent_Identity=28.8508557457213, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1788942, Length=404, Percent_Identity=29.2079207920792, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1789941, Length=408, Percent_Identity=31.3725490196078, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI87082231, Length=205, Percent_Identity=29.2682926829268, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082404, Length=384, Percent_Identity=24.21875, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46459; Mature: 46459

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS01319 RBFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METVAGLREGKRSFAVQERTKAVVAVIIGNGFEWFDFISYGFFSVIIAKIFFPTSDDNLS
CCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LLLSVSTIGVGFFMRPVGGIVLGGMADKIGRRATLVITITLMTIGTSLIAFAPTYKDAGI
EEEEEHHHHHHHHHHHHHHEEECCHHHHHCCCEEEEEEEEHHHHHHHHHHCCCCCCCCCH
FAPLMIVCARLLQGFSAGGEMGGATGFLRDNVPAERLGYYTSWIQASIGFAIILASVLAV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLVKVLSAEQVESWGWRIPFLIGLCLGPLGIYIRNQVHEPAEENVQIRERTPVLEIVRRW
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHCCCHHHHHHHH
KSETLIGFGLVIFWTVCSYVLLFYIPTYASKVLHLPASTGFIAVLVGASIVLFVTPVFGY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHH
LSDRYGRRRFLMGALAVAVMVAYPMFRLLNVSPGLHSLLLFQVVFGLVIACYEGPILAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
SDAFPRKIVSTGISISYNLAVITFGGFSAAILTWAIAATQNNLAPAFYVMGTAVLSFFAA
HHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ACWSPRKE
HHCCCCCC
>Mature Secondary Structure
METVAGLREGKRSFAVQERTKAVVAVIIGNGFEWFDFISYGFFSVIIAKIFFPTSDDNLS
CCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LLLSVSTIGVGFFMRPVGGIVLGGMADKIGRRATLVITITLMTIGTSLIAFAPTYKDAGI
EEEEEHHHHHHHHHHHHHHEEECCHHHHHCCCEEEEEEEEHHHHHHHHHHCCCCCCCCCH
FAPLMIVCARLLQGFSAGGEMGGATGFLRDNVPAERLGYYTSWIQASIGFAIILASVLAV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLVKVLSAEQVESWGWRIPFLIGLCLGPLGIYIRNQVHEPAEENVQIRERTPVLEIVRRW
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHCCCHHHHHHHH
KSETLIGFGLVIFWTVCSYVLLFYIPTYASKVLHLPASTGFIAVLVGASIVLFVTPVFGY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHH
LSDRYGRRRFLMGALAVAVMVAYPMFRLLNVSPGLHSLLLFQVVFGLVIACYEGPILAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
SDAFPRKIVSTGISISYNLAVITFGGFSAAILTWAIAATQNNLAPAFYVMGTAVLSFFAA
HHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ACWSPRKE
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]