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The map label for this gene is rluE [H]

Identifier: 83720677

GI number: 83720677

Start: 872365

End: 872943

Strand: Reverse

Name: rluE [H]

Synonym: BTH_I0758

Alternate gene names: 83720677

Gene position: 872943-872365 (Counterclockwise)

Preceding gene: 83720401

Following gene: 83721389

Centisome position: 22.92

GC content: 72.54

Gene sequence:

>579_bases
ATGCGCCTCATCGCCCTCAACAAACCGTTCGGCACGATCTGCCAGTTCTCCCCGCACGAAGCCCGCGCGTCGCTCGGCGA
CTGGGTGAAGACGCCCGGCGTCTATCCGGCGGGCCGTCTCGATGCCGACAGCGAGGGGCTGCTTCTGCTCACCGACGACG
GCGCGCTGCAGGCGAAGATCGCCGAGCCGCGCCACAAGCTCGTCAAGCGCTACTGGGCACAGGTCGAGGGCGCGCCCGAC
GCGGCCGCGCTCGCCGCGCTCGAGCGCGGCGTCGATCTCGGCGACTACGTGAGCCGCCCGTGCCGCGCGCGCTTCGTCAC
GCCGCCCGACACGCTCTGGCCGCGCACCCCGCCGATCCGCTATCGCGCGGCGATCCCGACCACCTGGATCGAGCTGGAGA
TCGCCGAAGGCAAGAACCGTCAGGTGCGGCGAATGACCGCGGCCATCGGCTTTCCGACGCTGCGCCTCATTCGCGTCGGC
ATCGGCGCGCTCGACGTGTTCGCGCTCGGCCTCGCGCCCGGCGAGACCCGCGAGCTGCCGCCGCGCGCGCCATGGGAGCG
CGCAGTGCGGCACGGATGA

Upstream 100 bases:

>100_bases
GACAAAAGACTTGCCGTTCCGTATTATGCATTAAGATTCCGCCATTTGCCATAGAACGCGGCCCGCGTCGCGCCGCCCGT
TTCCTCGCGAGCCTTGCCCC

Downstream 100 bases:

>100_bases
CAATTCGGCGGAACGTTTCGCCGTGCGTCAACCGACCGCGAACCCGACGCGTTAAACCACGCAGATGCCACTCCCAGCTA
TCCGGCATTGGCGACCGAGC

Product: pseudouridine synthase family protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MRLIALNKPFGTICQFSPHEARASLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQAKIAEPRHKLVKRYWAQVEGAPD
AAALAALERGVDLGDYVSRPCRARFVTPPDTLWPRTPPIRYRAAIPTTWIELEIAEGKNRQVRRMTAAIGFPTLRLIRVG
IGALDVFALGLAPGETRELPPRAPWERAVRHG

Sequences:

>Translated_192_residues
MRLIALNKPFGTICQFSPHEARASLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQAKIAEPRHKLVKRYWAQVEGAPD
AAALAALERGVDLGDYVSRPCRARFVTPPDTLWPRTPPIRYRAAIPTTWIELEIAEGKNRQVRRMTAAIGFPTLRLIRVG
IGALDVFALGLAPGETRELPPRAPWERAVRHG
>Mature_192_residues
MRLIALNKPFGTICQFSPHEARASLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQAKIAEPRHKLVKRYWAQVEGAPD
AAALAALERGVDLGDYVSRPCRARFVTPPDTLWPRTPPIRYRAAIPTTWIELEIAEGKNRQVRRMTAAIGFPTLRLIRVG
IGALDVFALGLAPGETRELPPRAPWERAVRHG

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=51.123595505618, Blast_Score=176, Evalue=7e-46,
Organism=Escherichia coli, GI1788510, Length=180, Percent_Identity=33.8888888888889, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790453, Length=178, Percent_Identity=29.7752808988764, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 21147; Mature: 21147

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIALNKPFGTICQFSPHEARASLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQAKI
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEC
AEPRHKLVKRYWAQVEGAPDAAALAALERGVDLGDYVSRPCRARFVTPPDTLWPRTPPIR
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCCEEECCCCCCCCCCCCCCE
YRAAIPTTWIELEIAEGKNRQVRRMTAAIGFPTLRLIRVGIGALDVFALGLAPGETRELP
EEEECCCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCCCCCCCCCC
PRAPWERAVRHG
CCCCHHHHHCCC
>Mature Secondary Structure
MRLIALNKPFGTICQFSPHEARASLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQAKI
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEC
AEPRHKLVKRYWAQVEGAPDAAALAALERGVDLGDYVSRPCRARFVTPPDTLWPRTPPIR
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCCEEECCCCCCCCCCCCCCE
YRAAIPTTWIELEIAEGKNRQVRRMTAAIGFPTLRLIRVGIGALDVFALGLAPGETRELP
EEEECCCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCCCCCCCCCC
PRAPWERAVRHG
CCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]