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The map label for this gene is cspA [H]

Identifier: 83720401

GI number: 83720401

Start: 876336

End: 876581

Strand: Reverse

Name: cspA [H]

Synonym: BTH_I0761

Alternate gene names: 83720401

Gene position: 876581-876336 (Counterclockwise)

Preceding gene: 83718436

Following gene: 83720677

Centisome position: 23.01

GC content: 53.25

Gene sequence:

>246_bases
GTGCTCGTTGTAAGTAGCGACTGTGTGACAGGGGAAGTAGGTATGGCAACTGGTATTGTCAAATGGTTCAATGACGCGAA
GGGCTTCGGTTTCATCACGCCCGACGAAGGCGGCGAAGATCTGTTTGCACATTTCTCGGCGATCAACATGCAGGGCTTCA
AGACCCTGAAGGAAGGCCAAAAGGTCAGCTTCGAGGTGGTGCAAGGCCCGAAGGGTAAGCAAGCGTCGAACATCCAAGCT
GCTTGA

Upstream 100 bases:

>100_bases
CTGGCACTTTTTTCAATTTGTCTTTGACAATCTGAAAGAGGCCGAGGTGAGCTTGTGAGGGGGGAACGGCTGCGTATGCC
GGTCGTTTAGCTTTTCGAGC

Downstream 100 bases:

>100_bases
GGGCCGTCTGGATCAGAAATAGAAAACCCGGCTTCCAGCCGGGTTTTTTTTCGCCCGAGTGCCGCGGCGAACAACCATGC
GCTCCACCACGGAACTCGGC

Product: cold-shock domain-contain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 81; Mature: 81

Protein sequence:

>81_residues
MLVVSSDCVTGEVGMATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQKVSFEVVQGPKGKQASNIQA
A

Sequences:

>Translated_81_residues
MLVVSSDCVTGEVGMATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQKVSFEVVQGPKGKQASNIQA
A
>Mature_81_residues
MLVVSSDCVTGEVGMATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQKVSFEVVQGPKGKQASNIQA
A

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=65.625, Blast_Score=92, Evalue=5e-21,
Organism=Escherichia coli, GI1786841, Length=63, Percent_Identity=61.9047619047619, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI1789979, Length=67, Percent_Identity=61.1940298507463, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=58.0645161290323, Blast_Score=82, Evalue=5e-18,
Organism=Escherichia coli, GI1788126, Length=63, Percent_Identity=61.9047619047619, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=57.8125, Blast_Score=79, Evalue=5e-17,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=56.4516129032258, Blast_Score=78, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983217, Length=56, Percent_Identity=51.7857142857143, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71983225, Length=56, Percent_Identity=51.7857142857143, Blast_Score=68, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24658520, Length=72, Percent_Identity=43.0555555555556, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 8599; Mature: 8599

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVVSSDCVTGEVGMATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQ
CEEECCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHCCC
KVSFEVVQGPKGKQASNIQAA
CEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MLVVSSDCVTGEVGMATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQ
CEEECCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHCCC
KVSFEVVQGPKGKQASNIQAA
CEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA