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The map label for this gene is hisS [H]

Identifier: 83720664

GI number: 83720664

Start: 2512375

End: 2513715

Strand: Direct

Name: hisS [H]

Synonym: BTH_I2235

Alternate gene names: 83720664

Gene position: 2512375-2513715 (Clockwise)

Preceding gene: 83718466

Following gene: 83720781

Centisome position: 65.96

GC content: 64.8

Gene sequence:

>1341_bases
ATGACAGAACAAAAGCGAAAGCTCGAGAAGCTGACGGGCGTGAAGGGCATGAACGACATCCTCCCGCAGGATGCCGGCTT
GTGGGAATTCTTCGAAGCGACGGTGAAGTCGCTGTTGCGCGCATACGGCTATCAGAATATCCGCACGCCGATCGTCGAGC
ACACGCCGCTCTTTACGCGCGGCATCGGCGAAGTGACCGACATCGTCGAAAAGGAAATGTACAGCTTTGTCGACGCGCTC
AACGGCGAGAACCTGACGCTGCGTCCCGAGAACACCGCGGCCGTCGTGCGCGCGGCGATCGAGCACAACATGCTGTACGA
CGGTCCGAAGCGACTGTGGTACATCGGGCCGATGTTCCGTCACGAGCGCCCGCAGCGCGGCCGCTATCGCCAGTTCCATC
AGGTCGGCGTCGAGGCGCTCGGCTTCGCGGGCCCGGACGCGGACGCGGAAATCGTCATGATGTGCCAGCGCCTGTGGGAG
GATCTCGGCCTCACCGGCATCAAGCTGGAGATCAACTCGCTCGGTCTCGCCGAGGAGCGCGCCGCGCACCGCGTCGAGCT
CATCAAGTATCTCGAGCAGCATGCGGACAAGCTCGACGACGACGCGCAGCGCCGCCTCTACACGAATCCGCTGCGTGTGC
TCGACACGAAGAACCCCGCGCTGCAGGAGATCGTGCGGAACGCGCCGAAACTGATCGACTTCCTCGGCGACGTGTCGCGC
GCGCACTTCGAAGGGCTGCAACGGCTGCTGAAGGCGAACAACGTGCCGTTCACGATCAATCCGCGCCTCGTGCGCGGGCT
CGATTATTACAACCTGACCGTGTTCGAGTGGGTGACCGACAAGCTCGGCGCGCAAGGCACGGTCGCCGCGGGCGGCCGCT
ACGATCCGCTGATCGAGCAACTGGGCGGCAAGCCGACCGCCGCGTGCGGCTGGGCGATGGGCATCGAGCGCATCCTTGAG
CTTCTGAAGGAAGAGCATCTCGTGCCGGAGCAGGAAGGCGTCGACGTGTACGTCGTCCACCAGGGCGACGCCGCGCGCGA
GCAGGCGTTCATCGTCGCCGAGCGTCTGCGCGACACGGGCCTCGACGTGATCCTGCATTGCAGCGCGGACGGCGCGGGCG
CGAGCTTCAAGTCGCAGATGAAGCGCGCGGACGCGAGCGGTGCGGCGTTCGCGGTGATCTTCGGCGAAGACGAGGTCACA
AACGGCACGGCGAGCGTGAAGCCGCTGCGCGGCACGGGCGACGACGGCGAAAAGAGCGTTCAGCAGTCCGTGCCGGTCGA
GAGCTTGACCGAATTTCTAATCAATGCGATGGTTGCAACCGCCGAAGACGGCGACGACTGA

Upstream 100 bases:

>100_bases
CGAGCGCATCGCGGAAGAATTCCAGCAGATCGTGAGCGACTACGTCGCGCGCACCTACGGCCGCGCCGCGGTCCAGAATT
AATTCGTCCACCGTATTCAG

Downstream 100 bases:

>100_bases
TCGCGGCGCATCCGCTCGACAAAGAAGCGTGTACAGGAAGGAATCGCTAGGCGATGAGTTATCACGACGAACAAGAATCG
ATTGAAAACCTGAAGGCGTG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTEQKRKLEKLTGVKGMNDILPQDAGLWEFFEATVKSLLRAYGYQNIRTPIVEHTPLFTRGIGEVTDIVEKEMYSFVDAL
NGENLTLRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFRHERPQRGRYRQFHQVGVEALGFAGPDADAEIVMMCQRLWE
DLGLTGIKLEINSLGLAEERAAHRVELIKYLEQHADKLDDDAQRRLYTNPLRVLDTKNPALQEIVRNAPKLIDFLGDVSR
AHFEGLQRLLKANNVPFTINPRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQLGGKPTAACGWAMGIERILE
LLKEEHLVPEQEGVDVYVVHQGDAAREQAFIVAERLRDTGLDVILHCSADGAGASFKSQMKRADASGAAFAVIFGEDEVT
NGTASVKPLRGTGDDGEKSVQQSVPVESLTEFLINAMVATAEDGDD

Sequences:

>Translated_446_residues
MTEQKRKLEKLTGVKGMNDILPQDAGLWEFFEATVKSLLRAYGYQNIRTPIVEHTPLFTRGIGEVTDIVEKEMYSFVDAL
NGENLTLRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFRHERPQRGRYRQFHQVGVEALGFAGPDADAEIVMMCQRLWE
DLGLTGIKLEINSLGLAEERAAHRVELIKYLEQHADKLDDDAQRRLYTNPLRVLDTKNPALQEIVRNAPKLIDFLGDVSR
AHFEGLQRLLKANNVPFTINPRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQLGGKPTAACGWAMGIERILE
LLKEEHLVPEQEGVDVYVVHQGDAAREQAFIVAERLRDTGLDVILHCSADGAGASFKSQMKRADASGAAFAVIFGEDEVT
NGTASVKPLRGTGDDGEKSVQQSVPVESLTEFLINAMVATAEDGDD
>Mature_445_residues
TEQKRKLEKLTGVKGMNDILPQDAGLWEFFEATVKSLLRAYGYQNIRTPIVEHTPLFTRGIGEVTDIVEKEMYSFVDALN
GENLTLRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFRHERPQRGRYRQFHQVGVEALGFAGPDADAEIVMMCQRLWED
LGLTGIKLEINSLGLAEERAAHRVELIKYLEQHADKLDDDAQRRLYTNPLRVLDTKNPALQEIVRNAPKLIDFLGDVSRA
HFEGLQRLLKANNVPFTINPRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQLGGKPTAACGWAMGIERILEL
LKEEHLVPEQEGVDVYVVHQGDAAREQAFIVAERLRDTGLDVILHCSADGAGASFKSQMKRADASGAAFAVIFGEDEVTN
GTASVKPLRGTGDDGEKSVQQSVPVESLTEFLINAMVATAEDGDD

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=406, Percent_Identity=55.4187192118227, Blast_Score=456, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 49589; Mature: 49458

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQKRKLEKLTGVKGMNDILPQDAGLWEFFEATVKSLLRAYGYQNIRTPIVEHTPLFTR
CCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
GIGEVTDIVEKEMYSFVDALNGENLTLRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFR
CHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCEEEECHHHH
HERPQRGRYRQFHQVGVEALGFAGPDADAEIVMMCQRLWEDLGLTGIKLEINSLGLAEER
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHH
AAHRVELIKYLEQHADKLDDDAQRRLYTNPLRVLDTKNPALQEIVRNAPKLIDFLGDVSR
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHH
AHFEGLQRLLKANNVPFTINPRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQ
HHHHHHHHHHHHCCCCEEECHHHHHCCCCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHH
LGGKPTAACGWAMGIERILELLKEEHLVPEQEGVDVYVVHQGDAAREQAFIVAERLRDTG
HCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
LDVILHCSADGAGASFKSQMKRADASGAAFAVIFGEDEVTNGTASVKPLRGTGDDGEKSV
CEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
QQSVPVESLTEFLINAMVATAEDGDD
HHCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TEQKRKLEKLTGVKGMNDILPQDAGLWEFFEATVKSLLRAYGYQNIRTPIVEHTPLFTR
CHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
GIGEVTDIVEKEMYSFVDALNGENLTLRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFR
CHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCEEEECHHHH
HERPQRGRYRQFHQVGVEALGFAGPDADAEIVMMCQRLWEDLGLTGIKLEINSLGLAEER
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHH
AAHRVELIKYLEQHADKLDDDAQRRLYTNPLRVLDTKNPALQEIVRNAPKLIDFLGDVSR
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHH
AHFEGLQRLLKANNVPFTINPRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQ
HHHHHHHHHHHHCCCCEEECHHHHHCCCCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHH
LGGKPTAACGWAMGIERILELLKEEHLVPEQEGVDVYVVHQGDAAREQAFIVAERLRDTG
HCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
LDVILHCSADGAGASFKSQMKRADASGAAFAVIFGEDEVTNGTASVKPLRGTGDDGEKSV
CEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
QQSVPVESLTEFLINAMVATAEDGDD
HHCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA