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The map label for this gene is ispG [H]

Identifier: 83718466

GI number: 83718466

Start: 2511052

End: 2512356

Strand: Direct

Name: ispG [H]

Synonym: BTH_I2234

Alternate gene names: 83718466

Gene position: 2511052-2512356 (Clockwise)

Preceding gene: 83721616

Following gene: 83720664

Centisome position: 65.92

GC content: 67.51

Gene sequence:

>1305_bases
ATGCAATCCGAAGCTCAATCCCCACGCAGCAGTCAGATTTGTTCAACCGAGCCGGTGTTCGGCGGGCATGCGCCGCGACG
CATGTCGCATGCGGTCGACGTCCGCTGGGGCGGCACGCTCGTGACGATCGGCGGCGCGGCGCCCGTGCGCGTGCAGTCGA
TGACGAACACCGACACGGCCGACGCGATCGGCACGGCGATCCAGGTGAAGGAACTCGCAAACGCGGGCTCCGAGCTCGTG
CGCATCACCGTGAACACGCCCGAGGCCGCCGCCGCCGTTCCGGCGATTCGCGAGCAGCTCGACCGGATGGGCGTGTCGGT
GCCGCTCGTCGGCGATTTCCACTACAACGGCCACCTGCTGCTGCGTGACTACCCGGGCTGCGCGGAGGCGCTGTCGAAGT
ACCGGATCAACCCGGGCAACGTCGGCCAGGGCGCGAAGCGCGATTCGCAGTTCGCGCAGATGATCGAAGCCGCGATCAAG
TACGACAAGCCGGTGCGGATCGGCGTGAACTGGGGCAGCCTCGATCAGGATCTGCTCGCGCGAATGATGGACGAGAACGG
CGCGCGCGCCGAGCCGTGGGAAGCGCAGAGCGTGATGTACGAGGCGCTGATCCAGTCGGCGATCGGCTCTGCCGAGCGCG
CGGTCGAACTGGGTCTCGGCCGCGACAAGATCGTGCTGTCGTGCAAGGTGAGCGGCGTGCAGGACTTGATCGCCGTGTAC
CGCGAGTTGTCGCGCCGCTGCGGTTTCGCACTGCACCTCGGTCTGACCGAGGCGGGCATGGGTTCGAAGGGTATCGTCGC
GTCGACGGCCGCGATCGGCGTGCTGCTGCAGGAAGGCATCGGCGACACGATCCGCATCTCGCTCACGCCGGAGCCGGGCG
CGTCGCGTACGGGCGAAGTGGTGGTCGGGCAGGAAATCCTGCAGACGATGGGGCTGCGCTCGTTCGCGCCGATGGTCGTC
GCATGCCCGGGGTGCGGGCGCACGACGAGCACGCTGTTCCAGGAGCTCGCGCTGCAGATCCAGACGTACCTGCGCGAGCA
GATGCCCGCGTGGCGCAAGGAATATCCGGGCGTCGAGAAGATGAACGTCGCGGTGATGGGCTGCATCGTCAACGGCCCGG
GCGAGTCGAAGCACGCGAACATCGGCATCAGCCTGCCGGGCTCGGGCGAGAATCCGGCCGCGCCCGTGTTCGTCGACGGC
GAAAAAGTGAAGACGCTGCGCGGCGAGCGCATCGCGGAAGAATTCCAGCAGATCGTGAGCGACTACGTCGCGCGCACCTA
CGGCCGCGCCGCGGTCCAGAATTAA

Upstream 100 bases:

>100_bases
ATTGCCCTGACGGCAAGCGCCGCGTCCGGCCAGGCGCGGCGCTTTTCCCTTTCGCGCGCCGCCGGGCAGGCGGCGCGTTT
GGCGTAAATTGGATCTATCG

Downstream 100 bases:

>100_bases
TTCGTCCACCGTATTCAGATGACAGAACAAAAGCGAAAGCTCGAGAAGCTGACGGGCGTGAAGGGCATGAACGACATCCT
CCCGCAGGATGCCGGCTTGT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MQSEAQSPRSSQICSTEPVFGGHAPRRMSHAVDVRWGGTLVTIGGAAPVRVQSMTNTDTADAIGTAIQVKELANAGSELV
RITVNTPEAAAAVPAIREQLDRMGVSVPLVGDFHYNGHLLLRDYPGCAEALSKYRINPGNVGQGAKRDSQFAQMIEAAIK
YDKPVRIGVNWGSLDQDLLARMMDENGARAEPWEAQSVMYEALIQSAIGSAERAVELGLGRDKIVLSCKVSGVQDLIAVY
RELSRRCGFALHLGLTEAGMGSKGIVASTAAIGVLLQEGIGDTIRISLTPEPGASRTGEVVVGQEILQTMGLRSFAPMVV
ACPGCGRTTSTLFQELALQIQTYLREQMPAWRKEYPGVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFVDG
EKVKTLRGERIAEEFQQIVSDYVARTYGRAAVQN

Sequences:

>Translated_434_residues
MQSEAQSPRSSQICSTEPVFGGHAPRRMSHAVDVRWGGTLVTIGGAAPVRVQSMTNTDTADAIGTAIQVKELANAGSELV
RITVNTPEAAAAVPAIREQLDRMGVSVPLVGDFHYNGHLLLRDYPGCAEALSKYRINPGNVGQGAKRDSQFAQMIEAAIK
YDKPVRIGVNWGSLDQDLLARMMDENGARAEPWEAQSVMYEALIQSAIGSAERAVELGLGRDKIVLSCKVSGVQDLIAVY
RELSRRCGFALHLGLTEAGMGSKGIVASTAAIGVLLQEGIGDTIRISLTPEPGASRTGEVVVGQEILQTMGLRSFAPMVV
ACPGCGRTTSTLFQELALQIQTYLREQMPAWRKEYPGVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFVDG
EKVKTLRGERIAEEFQQIVSDYVARTYGRAAVQN
>Mature_434_residues
MQSEAQSPRSSQICSTEPVFGGHAPRRMSHAVDVRWGGTLVTIGGAAPVRVQSMTNTDTADAIGTAIQVKELANAGSELV
RITVNTPEAAAAVPAIREQLDRMGVSVPLVGDFHYNGHLLLRDYPGCAEALSKYRINPGNVGQGAKRDSQFAQMIEAAIK
YDKPVRIGVNWGSLDQDLLARMMDENGARAEPWEAQSVMYEALIQSAIGSAERAVELGLGRDKIVLSCKVSGVQDLIAVY
RELSRRCGFALHLGLTEAGMGSKGIVASTAAIGVLLQEGIGDTIRISLTPEPGASRTGEVVVGQEILQTMGLRSFAPMVV
ACPGCGRTTSTLFQELALQIQTYLREQMPAWRKEYPGVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFVDG
EKVKTLRGERIAEEFQQIVSDYVARTYGRAAVQN

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=376, Percent_Identity=33.7765957446808, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 46362; Mature: 46362

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSEAQSPRSSQICSTEPVFGGHAPRRMSHAVDVRWGGTLVTIGGAAPVRVQSMTNTDTA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEEEECCEEEEECCCCCEEEEECCCCCHH
DAIGTAIQVKELANAGSELVRITVNTPEAAAAVPAIREQLDRMGVSVPLVGDFHYNGHLL
HHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEE
LRDYPGCAEALSKYRINPGNVGQGAKRDSQFAQMIEAAIKYDKPVRIGVNWGSLDQDLLA
EECCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHH
RMMDENGARAEPWEAQSVMYEALIQSAIGSAERAVELGLGRDKIVLSCKVSGVQDLIAVY
HHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
RELSRRCGFALHLGLTEAGMGSKGIVASTAAIGVLLQEGIGDTIRISLTPEPGASRTGEV
HHHHHHCCEEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCE
VVGQEILQTMGLRSFAPMVVACPGCGRTTSTLFQELALQIQTYLREQMPAWRKEYPGVEK
EECHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
MNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFVDGEKVKTLRGERIAEEFQQIVS
EEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHH
DYVARTYGRAAVQN
HHHHHHHHHHHCCC
>Mature Secondary Structure
MQSEAQSPRSSQICSTEPVFGGHAPRRMSHAVDVRWGGTLVTIGGAAPVRVQSMTNTDTA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEEEECCEEEEECCCCCEEEEECCCCCHH
DAIGTAIQVKELANAGSELVRITVNTPEAAAAVPAIREQLDRMGVSVPLVGDFHYNGHLL
HHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEE
LRDYPGCAEALSKYRINPGNVGQGAKRDSQFAQMIEAAIKYDKPVRIGVNWGSLDQDLLA
EECCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHH
RMMDENGARAEPWEAQSVMYEALIQSAIGSAERAVELGLGRDKIVLSCKVSGVQDLIAVY
HHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
RELSRRCGFALHLGLTEAGMGSKGIVASTAAIGVLLQEGIGDTIRISLTPEPGASRTGEV
HHHHHHCCEEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCE
VVGQEILQTMGLRSFAPMVVACPGCGRTTSTLFQELALQIQTYLREQMPAWRKEYPGVEK
EECHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
MNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFVDGEKVKTLRGERIAEEFQQIVS
EEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHH
DYVARTYGRAAVQN
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA