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The map label for this gene is fliM [H]

Identifier: 83720285

GI number: 83720285

Start: 28509

End: 29507

Strand: Direct

Name: fliM [H]

Synonym: BTH_I0027

Alternate gene names: 83720285

Gene position: 28509-29507 (Clockwise)

Preceding gene: 83720504

Following gene: 83721171

Centisome position: 0.75

GC content: 63.06

Gene sequence:

>999_bases
ATGGGCCACGAAGAATTCATGTCCCAGGAGGAGGTGGATGCACTCCTCAAGGGCGTCACGGGCGAAGACGACTCTGCCGA
CGAACCGGCCGAGTCGTCCGGCATCCGCCCCTACAACATCGCGACGCAGGAGCGGATCGTCCGCGGCCGGATGCCCGGCC
TCGAGATCATCAACGATCGTTTCGCGCGCCTGCTGCGGATCGGCATCTTCAACTTCATGCGGCGCACGGCGGAGATCTCC
GTGAGCCAGGTGAAGGTGCAGAAGTACAGCGAGTTCACCCGCAACCTGCCGATTCCGACCAATCTGAACCTCGTGCACGT
GAAGCCGCTGCGCGGCACGTCGCTGTTCGTGTTCGATCCGAACCTCGTGTTCTTCGTCGTCGACAACCTGTTCGGCGGCG
ACGGGCGTTTCCACACGCGCGTCGAGGGCCGCGACTTCACGGCGACCGAGCAGCGGATCATCGGCAAGCTGCTCAATCTC
GTGTTCGAGCACTACGCGGTTGCATGGAAAAGCGTGCGGCCGCTGCAGTTCGAGTTCGTGCGCTCGGAGATGCACACGCA
GTTCGCGAACGTCGCGACGCCCAACGAGATCGTGATCGTCACGCAGTTCTCGATCGAGTTCGGGCCGACGGGCGGCACGC
TGCACATCTGCATGCCGTACTCGATGATCGAGCCGATCCGCGATGTGCTTTCCTCGCCGATCCAGGGCGAGGCGCTCGAG
GTGGACCGCCGCTGGGTGCGCGTGCTGTCGCAGCAGGTGCAATCGGCGGAAGTGGAACTGGTCGCGGATCTCGCCGAGGT
CCCGACGACGTTCGAGAAGATTCTCAACCTGCGCGCGGGCGACGTGCTGCCGCTCGACATCGCGGATTCGATCACCGCGA
AGGTCGACGGCGTGCCGGTGATGGAGTGCGGTTACGGCATTTTCAATGGCCAGTACGCGTTGCGCGTGCAGAAAATGATC
AGCGCAAGCGACACGATGAAGGAAGGTGGATATGACTGA

Upstream 100 bases:

>100_bases
ATCGAGCAGCCGACCCAGCCGGGCAATCAGAGCGCACGCGTCGACGACGTGCTGTTCACCGAGTTCGTCGTCCAATAACG
CCGCCGGCAAAGGAGCGCGC

Downstream 100 bases:

>100_bases
GTTGAACGCGACCCCCGAGGCCGACGGCATCGATGAACAGGCGTTTGCGGACGCCGCGATGAGCGCCGCCGCGGCCGACG
GCGCGGCACCCGCCGCGCCG

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MGHEEFMSQEEVDALLKGVTGEDDSADEPAESSGIRPYNIATQERIVRGRMPGLEIINDRFARLLRIGIFNFMRRTAEIS
VSQVKVQKYSEFTRNLPIPTNLNLVHVKPLRGTSLFVFDPNLVFFVVDNLFGGDGRFHTRVEGRDFTATEQRIIGKLLNL
VFEHYAVAWKSVRPLQFEFVRSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALE
VDRRWVRVLSQQVQSAEVELVADLAEVPTTFEKILNLRAGDVLPLDIADSITAKVDGVPVMECGYGIFNGQYALRVQKMI
SASDTMKEGGYD

Sequences:

>Translated_332_residues
MGHEEFMSQEEVDALLKGVTGEDDSADEPAESSGIRPYNIATQERIVRGRMPGLEIINDRFARLLRIGIFNFMRRTAEIS
VSQVKVQKYSEFTRNLPIPTNLNLVHVKPLRGTSLFVFDPNLVFFVVDNLFGGDGRFHTRVEGRDFTATEQRIIGKLLNL
VFEHYAVAWKSVRPLQFEFVRSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALE
VDRRWVRVLSQQVQSAEVELVADLAEVPTTFEKILNLRAGDVLPLDIADSITAKVDGVPVMECGYGIFNGQYALRVQKMI
SASDTMKEGGYD
>Mature_331_residues
GHEEFMSQEEVDALLKGVTGEDDSADEPAESSGIRPYNIATQERIVRGRMPGLEIINDRFARLLRIGIFNFMRRTAEISV
SQVKVQKYSEFTRNLPIPTNLNLVHVKPLRGTSLFVFDPNLVFFVVDNLFGGDGRFHTRVEGRDFTATEQRIIGKLLNLV
FEHYAVAWKSVRPLQFEFVRSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALEV
DRRWVRVLSQQVQSAEVELVADLAEVPTTFEKILNLRAGDVLPLDIADSITAKVDGVPVMECGYGIFNGQYALRVQKMIS
ASDTMKEGGYD

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=326, Percent_Identity=51.840490797546, Blast_Score=350, Evalue=7e-98,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37275; Mature: 37144

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHEEFMSQEEVDALLKGVTGEDDSADEPAESSGIRPYNIATQERIVRGRMPGLEIINDR
CCHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCEECHHHHHHHHCCCCCHHHHHHH
FARLLRIGIFNFMRRTAEISVSQVKVQKYSEFTRNLPIPTNLNLVHVKPLRGTSLFVFDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEECC
NLVFFVVDNLFGGDGRFHTRVEGRDFTATEQRIIGKLLNLVFEHYAVAWKSVRPLQFEFV
CEEEEEEEHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
RSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALE
HHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCHHH
VDRRWVRVLSQQVQSAEVELVADLAEVPTTFEKILNLRAGDVLPLDIADSITAKVDGVPV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCCHHHHCCCCEE
MECGYGIFNGQYALRVQKMISASDTMKEGGYD
HHCCCCEECCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GHEEFMSQEEVDALLKGVTGEDDSADEPAESSGIRPYNIATQERIVRGRMPGLEIINDR
CHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCEECHHHHHHHHCCCCCHHHHHHH
FARLLRIGIFNFMRRTAEISVSQVKVQKYSEFTRNLPIPTNLNLVHVKPLRGTSLFVFDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEECC
NLVFFVVDNLFGGDGRFHTRVEGRDFTATEQRIIGKLLNLVFEHYAVAWKSVRPLQFEFV
CEEEEEEEHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
RSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALE
HHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCHHH
VDRRWVRVLSQQVQSAEVELVADLAEVPTTFEKILNLRAGDVLPLDIADSITAKVDGVPV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCCHHHHCCCCEE
MECGYGIFNGQYALRVQKMISASDTMKEGGYD
HHCCCCEECCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3519573; 9097040; 9278503; 2651416 [H]