| Definition | Burkholderia thailandensis E264 chromosome chromosome I, complete sequence. |
|---|---|
| Accession | NC_007651 |
| Length | 3,809,201 |
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The map label for this gene is fabG [H]
Identifier: 83720260
GI number: 83720260
Start: 1000785
End: 1001543
Strand: Reverse
Name: fabG [H]
Synonym: BTH_I0876
Alternate gene names: 83720260
Gene position: 1001543-1000785 (Counterclockwise)
Preceding gene: 83721175
Following gene: 83719387
Centisome position: 26.29
GC content: 70.22
Gene sequence:
>759_bases ATGCGGCTCAGCGGCAAGACGGCCGTCGTCACGGGCGCAGGCTCGGGCTTCGGCGAAGGCATTGCGAAGACGTTTGCACG CGAAGGCGCGTGCGTCGTCGTCAACGATCTGAATGCGGCGGCGGCCGAGCGCGTTGCGGGCGACATCGCGCTCGCGGGCG GCCGCGCGCTCGCCGTCGCGGGCGACGTGTCGCGCGGCGAAGACTGGCAGACGCTGCGCGACGCCGCGCTCGACGCGTTC GGCAGCGTGCAGGTCGTCGTCAACAACGCGGGCACCACGCACCGCAACAAGCCGGTGCTCGACATCGCCGAAGCCGAATA CGACCGCGTGTACGCGGTCAACATGAAGAGCCTCTTCTGGAGCGTGCAGACGTTCGTCCCGCATTTCCGCGCCGCGGGCA GCGGCGCGTTCGTGAACGTCGCGTCGACGGCGGGCGTGCGGCCGCGCCCGGGCCTCGTCTGGTACAACAGCACGAAGGGC GCGATGATCACCGCGAGCAAGACGCTCGCCGTCGAGCTCGGAGCCGACCGGATTCGCGTGAACTGCGTGAACCCGGTGCT CGGCGAAACCGGCCTCACGAGCGAATTCATGGGCGTGCCCGACACGCCGGAGAACCGCGCGCGCTTCGTCGCGACAATCC CGCTCGGCCGCCTGTCGACGCCCCAGGACATCGCGAACGCGGCGCTCTATCTCGCGTCCGACGAAGCCGCGTTCGTCACC GGCATCTGCGTCGAGGTCGACGGCGGCCGCTGCGTCTGA
Upstream 100 bases:
>100_bases ACACGGCTGAATCGGGCGGCGCGGCGCGCATGCGTGTCGCGCCGCCCGCCGCCGTCGAGGCGGCGCCCCGGCATTCACGA CAAAATCCAGGAGACAACCG
Downstream 100 bases:
>100_bases GCGCCGAACACTAGACAACACACCAGAGGAGACAGTCATGGCCCGTACCATGCCGGCGGCCACGCCGGGCGCGCCGTCCG CTTTCGAGGCGGCCACTTAT
Product: 3-ketoacyl-(acyl-carrier-protein) reductase
Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]
Alternate protein names: 2,5-DDOL dehydrogenase [H]
Number of amino acids: Translated: 252; Mature: 252
Protein sequence:
>252_residues MRLSGKTAVVTGAGSGFGEGIAKTFAREGACVVVNDLNAAAAERVAGDIALAGGRALAVAGDVSRGEDWQTLRDAALDAF GSVQVVVNNAGTTHRNKPVLDIAEAEYDRVYAVNMKSLFWSVQTFVPHFRAAGSGAFVNVASTAGVRPRPGLVWYNSTKG AMITASKTLAVELGADRIRVNCVNPVLGETGLTSEFMGVPDTPENRARFVATIPLGRLSTPQDIANAALYLASDEAAFVT GICVEVDGGRCV
Sequences:
>Translated_252_residues MRLSGKTAVVTGAGSGFGEGIAKTFAREGACVVVNDLNAAAAERVAGDIALAGGRALAVAGDVSRGEDWQTLRDAALDAF GSVQVVVNNAGTTHRNKPVLDIAEAEYDRVYAVNMKSLFWSVQTFVPHFRAAGSGAFVNVASTAGVRPRPGLVWYNSTKG AMITASKTLAVELGADRIRVNCVNPVLGETGLTSEFMGVPDTPENRARFVATIPLGRLSTPQDIANAALYLASDEAAFVT GICVEVDGGRCV >Mature_252_residues MRLSGKTAVVTGAGSGFGEGIAKTFAREGACVVVNDLNAAAAERVAGDIALAGGRALAVAGDVSRGEDWQTLRDAALDAF GSVQVVVNNAGTTHRNKPVLDIAEAEYDRVYAVNMKSLFWSVQTFVPHFRAAGSGAFVNVASTAGVRPRPGLVWYNSTKG AMITASKTLAVELGADRIRVNCVNPVLGETGLTSEFMGVPDTPENRARFVATIPLGRLSTPQDIANAALYLASDEAAFVT GICVEVDGGRCV
Specific function: Catalyzes the degradation of 2,5-dichloro-2,5- cyclohexadiene-1,4-diol (2,5-DDOL) into 2,5-dichlorohydroquinone (2,5-DCHQ). LinX appears not to be involved in gamma-HCH degradation pathway [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI32483357, Length=250, Percent_Identity=32.4, Blast_Score=121, Evalue=6e-28, Organism=Homo sapiens, GI126723750, Length=253, Percent_Identity=30.4347826086957, Blast_Score=108, Evalue=3e-24, Organism=Homo sapiens, GI15277342, Length=257, Percent_Identity=33.0739299610895, Blast_Score=107, Evalue=9e-24, Organism=Homo sapiens, GI5031737, Length=249, Percent_Identity=32.5301204819277, Blast_Score=107, Evalue=1e-23, Organism=Homo sapiens, GI7705925, Length=248, Percent_Identity=31.8548387096774, Blast_Score=105, Evalue=3e-23, Organism=Homo sapiens, GI66933014, Length=250, Percent_Identity=32.8, Blast_Score=104, Evalue=6e-23, Organism=Homo sapiens, GI33667109, Length=228, Percent_Identity=32.0175438596491, Blast_Score=96, Evalue=4e-20, Organism=Homo sapiens, GI4504505, Length=194, Percent_Identity=31.9587628865979, Blast_Score=94, Evalue=1e-19, Organism=Homo sapiens, GI19923817, Length=257, Percent_Identity=27.6264591439689, Blast_Score=93, Evalue=3e-19, Organism=Homo sapiens, GI126723191, Length=185, Percent_Identity=31.8918918918919, Blast_Score=91, Evalue=8e-19, Organism=Homo sapiens, GI40254992, Length=244, Percent_Identity=29.5081967213115, Blast_Score=91, Evalue=1e-18, Organism=Homo sapiens, GI142976729, Length=242, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=1e-17, Organism=Homo sapiens, GI210032110, Length=196, Percent_Identity=31.1224489795918, Blast_Score=87, Evalue=2e-17, Organism=Homo sapiens, GI10190704, Length=253, Percent_Identity=31.2252964426877, Blast_Score=86, Evalue=3e-17, Organism=Homo sapiens, GI59889578, Length=230, Percent_Identity=28.695652173913, Blast_Score=84, Evalue=9e-17, Organism=Homo sapiens, GI40807363, Length=193, Percent_Identity=30.0518134715026, Blast_Score=83, Evalue=2e-16, Organism=Homo sapiens, GI310128830, Length=201, Percent_Identity=29.3532338308458, Blast_Score=78, Evalue=6e-15, Organism=Homo sapiens, GI310115468, Length=201, Percent_Identity=29.3532338308458, Blast_Score=78, Evalue=6e-15, Organism=Homo sapiens, GI25282469, Length=218, Percent_Identity=26.605504587156, Blast_Score=75, Evalue=6e-14, Organism=Homo sapiens, GI302344755, Length=109, Percent_Identity=32.1100917431193, Blast_Score=69, Evalue=6e-12, Organism=Homo sapiens, GI4758504, Length=259, Percent_Identity=27.7992277992278, Blast_Score=68, Evalue=7e-12, Organism=Homo sapiens, GI31542939, Length=253, Percent_Identity=25.6916996047431, Blast_Score=67, Evalue=1e-11, Organism=Homo sapiens, GI109715829, Length=248, Percent_Identity=27.0161290322581, Blast_Score=65, Evalue=8e-11, Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=37.3493975903614, Blast_Score=144, Evalue=7e-36, Organism=Escherichia coli, GI87082100, Length=252, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=2e-29, Organism=Escherichia coli, GI1787335, Length=249, Percent_Identity=32.5301204819277, Blast_Score=116, Evalue=1e-27, Organism=Escherichia coli, GI1789208, Length=248, Percent_Identity=30.241935483871, Blast_Score=110, Evalue=8e-26, Organism=Escherichia coli, GI1790717, Length=250, Percent_Identity=28.4, Blast_Score=106, Evalue=2e-24, Organism=Escherichia coli, GI1789378, Length=248, Percent_Identity=29.8387096774194, Blast_Score=103, Evalue=1e-23, Organism=Escherichia coli, GI2367175, Length=249, Percent_Identity=32.9317269076305, Blast_Score=101, Evalue=5e-23, Organism=Escherichia coli, GI1786812, Length=268, Percent_Identity=32.089552238806, Blast_Score=96, Evalue=3e-21, Organism=Escherichia coli, GI87082160, Length=260, Percent_Identity=30, Blast_Score=94, Evalue=7e-21, Organism=Escherichia coli, GI1788459, Length=246, Percent_Identity=26.8292682926829, Blast_Score=80, Evalue=2e-16, Organism=Escherichia coli, GI1787526, Length=251, Percent_Identity=27.0916334661355, Blast_Score=67, Evalue=1e-12, Organism=Escherichia coli, GI1789057, Length=261, Percent_Identity=24.904214559387, Blast_Score=64, Evalue=8e-12, Organism=Caenorhabditis elegans, GI17561402, Length=249, Percent_Identity=30.9236947791165, Blast_Score=130, Evalue=5e-31, Organism=Caenorhabditis elegans, GI17555706, Length=248, Percent_Identity=37.9032258064516, Blast_Score=127, Evalue=7e-30, Organism=Caenorhabditis elegans, GI25147288, Length=249, Percent_Identity=33.7349397590361, Blast_Score=115, Evalue=3e-26, Organism=Caenorhabditis elegans, GI17562906, Length=261, Percent_Identity=31.8007662835249, Blast_Score=114, Evalue=5e-26, Organism=Caenorhabditis elegans, GI71994604, Length=248, Percent_Identity=34.2741935483871, Blast_Score=113, Evalue=1e-25, Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=31.8181818181818, Blast_Score=112, Evalue=2e-25, Organism=Caenorhabditis elegans, GI17562904, Length=269, Percent_Identity=30.8550185873606, Blast_Score=110, Evalue=7e-25, Organism=Caenorhabditis elegans, GI17560676, Length=247, Percent_Identity=28.7449392712551, Blast_Score=109, Evalue=2e-24, Organism=Caenorhabditis elegans, GI71994600, Length=254, Percent_Identity=31.496062992126, Blast_Score=108, Evalue=3e-24, Organism=Caenorhabditis elegans, GI17531453, Length=261, Percent_Identity=29.8850574712644, Blast_Score=106, Evalue=1e-23, Organism=Caenorhabditis elegans, GI17538480, Length=269, Percent_Identity=30.4832713754647, Blast_Score=104, Evalue=4e-23, Organism=Caenorhabditis elegans, GI17559104, Length=266, Percent_Identity=30.0751879699248, Blast_Score=104, Evalue=4e-23, Organism=Caenorhabditis elegans, GI115534694, Length=248, Percent_Identity=30.241935483871, Blast_Score=103, Evalue=7e-23, Organism=Caenorhabditis elegans, GI17560332, Length=263, Percent_Identity=30.4182509505703, Blast_Score=103, Evalue=1e-22, Organism=Caenorhabditis elegans, GI17560150, Length=264, Percent_Identity=29.1666666666667, Blast_Score=103, Evalue=1e-22, Organism=Caenorhabditis elegans, GI17562910, Length=264, Percent_Identity=29.9242424242424, Blast_Score=102, Evalue=2e-22, Organism=Caenorhabditis elegans, GI193204405, Length=265, Percent_Identity=29.811320754717, Blast_Score=102, Evalue=2e-22, Organism=Caenorhabditis elegans, GI17564282, Length=200, Percent_Identity=31.5, Blast_Score=100, Evalue=8e-22, Organism=Caenorhabditis elegans, GI17562990, Length=259, Percent_Identity=29.3436293436293, Blast_Score=97, Evalue=1e-20, Organism=Caenorhabditis elegans, GI17568967, Length=214, Percent_Identity=33.1775700934579, Blast_Score=96, Evalue=3e-20, Organism=Caenorhabditis elegans, GI17544670, Length=265, Percent_Identity=28.3018867924528, Blast_Score=95, Evalue=4e-20, Organism=Caenorhabditis elegans, GI17560220, Length=264, Percent_Identity=29.5454545454545, Blast_Score=93, Evalue=1e-19, Organism=Caenorhabditis elegans, GI17538486, Length=263, Percent_Identity=27.3764258555133, Blast_Score=93, Evalue=1e-19, Organism=Caenorhabditis elegans, GI17551412, Length=269, Percent_Identity=28.996282527881, Blast_Score=93, Evalue=1e-19, Organism=Caenorhabditis elegans, GI17565030, Length=264, Percent_Identity=28.4090909090909, Blast_Score=91, Evalue=6e-19, Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=27.7566539923954, Blast_Score=90, Evalue=9e-19, Organism=Caenorhabditis elegans, GI17508895, Length=171, Percent_Identity=31.5789473684211, Blast_Score=87, Evalue=6e-18, Organism=Caenorhabditis elegans, GI193203117, Length=194, Percent_Identity=29.3814432989691, Blast_Score=85, Evalue=4e-17, Organism=Caenorhabditis elegans, GI193203115, Length=190, Percent_Identity=29.4736842105263, Blast_Score=81, Evalue=5e-16, Organism=Caenorhabditis elegans, GI17563726, Length=265, Percent_Identity=26.0377358490566, Blast_Score=79, Evalue=3e-15, Organism=Caenorhabditis elegans, GI17536651, Length=259, Percent_Identity=28.957528957529, Blast_Score=68, Evalue=6e-12, Organism=Caenorhabditis elegans, GI17567345, Length=196, Percent_Identity=26.0204081632653, Blast_Score=66, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17508651, Length=251, Percent_Identity=24.3027888446215, Blast_Score=65, Evalue=3e-11, Organism=Saccharomyces cerevisiae, GI6322861, Length=195, Percent_Identity=32.3076923076923, Blast_Score=87, Evalue=2e-18, Organism=Saccharomyces cerevisiae, GI6323882, Length=199, Percent_Identity=27.6381909547739, Blast_Score=64, Evalue=2e-11, Organism=Drosophila melanogaster, GI24644339, Length=255, Percent_Identity=34.1176470588235, Blast_Score=134, Evalue=4e-32, Organism=Drosophila melanogaster, GI23397609, Length=252, Percent_Identity=34.9206349206349, Blast_Score=131, Evalue=5e-31, Organism=Drosophila melanogaster, GI24639444, Length=250, Percent_Identity=35.2, Blast_Score=124, Evalue=4e-29, Organism=Drosophila melanogaster, GI21357041, Length=250, Percent_Identity=35.6, Blast_Score=120, Evalue=7e-28, Organism=Drosophila melanogaster, GI24643142, Length=250, Percent_Identity=32.8, Blast_Score=115, Evalue=4e-26, Organism=Drosophila melanogaster, GI28571526, Length=250, Percent_Identity=33.6, Blast_Score=113, Evalue=1e-25, Organism=Drosophila melanogaster, GI21355319, Length=253, Percent_Identity=32.0158102766798, Blast_Score=111, Evalue=4e-25, Organism=Drosophila melanogaster, GI24644337, Length=258, Percent_Identity=31.7829457364341, Blast_Score=96, Evalue=2e-20, Organism=Drosophila melanogaster, GI24649181, Length=188, Percent_Identity=33.5106382978723, Blast_Score=96, Evalue=2e-20, Organism=Drosophila melanogaster, GI24640492, Length=194, Percent_Identity=29.3814432989691, Blast_Score=93, Evalue=2e-19, Organism=Drosophila melanogaster, GI18859837, Length=194, Percent_Identity=32.9896907216495, Blast_Score=92, Evalue=3e-19, Organism=Drosophila melanogaster, GI281364892, Length=200, Percent_Identity=31.5, Blast_Score=92, Evalue=4e-19, Organism=Drosophila melanogaster, GI21355631, Length=215, Percent_Identity=29.3023255813954, Blast_Score=75, Evalue=5e-14, Organism=Drosophila melanogaster, GI24581726, Length=196, Percent_Identity=29.5918367346939, Blast_Score=74, Evalue=7e-14, Organism=Drosophila melanogaster, GI21357673, Length=212, Percent_Identity=26.8867924528302, Blast_Score=74, Evalue=1e-13, Organism=Drosophila melanogaster, GI17737361, Length=256, Percent_Identity=27.734375, Blast_Score=69, Evalue=3e-12, Organism=Drosophila melanogaster, GI221510726, Length=229, Percent_Identity=27.0742358078603, Blast_Score=69, Evalue=4e-12, Organism=Drosophila melanogaster, GI161076933, Length=191, Percent_Identity=26.7015706806283, Blast_Score=68, Evalue=7e-12, Organism=Drosophila melanogaster, GI24584748, Length=191, Percent_Identity=26.7015706806283, Blast_Score=67, Evalue=8e-12, Organism=Drosophila melanogaster, GI21358495, Length=199, Percent_Identity=28.1407035175879, Blast_Score=64, Evalue=6e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.159 [C]
Molecular weight: Translated: 26204; Mature: 26204
Theoretical pI: Translated: 5.84; Mature: 5.84
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 1.6 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 1.6 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRLSGKTAVVTGAGSGFGEGIAKTFAREGACVVVNDLNAAAAERVAGDIALAGGRALAVA CCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHCEEEEECCCEEEEE GDVSRGEDWQTLRDAALDAFGSVQVVVNNAGTTHRNKPVLDIAEAEYDRVYAVNMKSLFW CCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEECCCCCCEEEEEEHHHHHH SVQTFVPHFRAAGSGAFVNVASTAGVRPRPGLVWYNSTKGAMITASKTLAVELGADRIRV HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEECCEEEEEECCCEEEE NCVNPVLGETGLTSEFMGVPDTPENRARFVATIPLGRLSTPQDIANAALYLASDEAAFVT EECCCCCCCCCCCHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHCEEEEEECCCCEEEE GICVEVDGGRCV EEEEEECCCCCC >Mature Secondary Structure MRLSGKTAVVTGAGSGFGEGIAKTFAREGACVVVNDLNAAAAERVAGDIALAGGRALAVA CCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHCEEEEECCCEEEEE GDVSRGEDWQTLRDAALDAFGSVQVVVNNAGTTHRNKPVLDIAEAEYDRVYAVNMKSLFW CCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEECCCCCCEEEEEEHHHHHH SVQTFVPHFRAAGSGAFVNVASTAGVRPRPGLVWYNSTKGAMITASKTLAVELGADRIRV HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEECCEEEEEECCCEEEE NCVNPVLGETGLTSEFMGVPDTPENRARFVATIPLGRLSTPQDIANAALYLASDEAAFVT EECCCCCCCCCCCHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHCEEEEEECCCCEEEE GICVEVDGGRCV EEEEEECCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]
Metal ions: CaCl2; KCl; MnCl2; NaCl [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]
Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]
Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]
General reaction: Redox reaction [C]
Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 7515041; 12450824 [H]