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The map label for this gene is ynfL [H]

Identifier: 83720162

GI number: 83720162

Start: 3063221

End: 3064117

Strand: Reverse

Name: ynfL [H]

Synonym: BTH_I2683

Alternate gene names: 83720162

Gene position: 3064117-3063221 (Counterclockwise)

Preceding gene: 83721670

Following gene: 83718598

Centisome position: 80.44

GC content: 66.78

Gene sequence:

>897_bases
ATGATGGAATCCAAGCATCTACGTTATTTCCTGGCCATCGCCGATACCGGCAGTCTCACTCACGCGGCGATTCGCCTGGG
TGTCGCGCAGCCCGCGCTCAGTCATGCGCTGACGAAAATGGAGCGCGAGCTCGGCGTGAAGCTGTTCGACCGTTCGAGGC
GCGGCGCGACGCTGACGACGGCGGGCGAAGCGATCGTCGACGACATTCGTCTGAGCCTCGCGCGGATGGACGCAGCCGCG
CGGCGCGCGAGCGAGATCGGCGCGCAGCGCGCCGGGCGGCTCAGCATCGGCTTCGTGTCGGCCGCACTGTTCGATACATT
GCCTGCCGCGGTGTGCGCGTTGCGCGCGGCGTCCCCGCGCGTCGATCTCGTGATGAGGGAGATGAGCAATGCGGAGCAAG
TTGTCGCGCTCGAGCGAGGCGAGATCGATATCGGCATACTGCATACGCCTGTCGCGATCGGCGGCCCTGTTCACGAGAAG
CTGATCCGGCGCGATCCGCTGATCGCCGTGATTCCGAAGTCGTTTCCGCGGCAGCCGGACGGCTCGATCACGCTGCGGGA
GCTGTCTCGCGCGGGGCTCATCTGGTTTCCGCACGAGCAGTTGCCGTCGATTCGCGCGGCGATCCTCAGCGCGTTCCGGC
TGGCGGGGCACCCGATCGAGGTCGTCCAGGACGCCAATCGCACGCTGACTGTGATCGCCTGTGTGGCGGCTGGCTGCGGC
GTGTCGCTACTGCCGTCGTCGATCCGGACGCTGACGTTCGAAGGCGTCGACTTCAGCCCGGTGGCTGACGGGCAGGCGCT
GCCGGAATTTCCGTTGACGGCGATCTGGCCCAAGCGGTCGCGCCGCACGCTCGCCGATCGCTTCGCGGAGCTATTGCCCA
GGTTTCAGCCGAGTTGA

Upstream 100 bases:

>100_bases
TTGGGAGAGCGTGCGGAATGAAGGCTGGCGTTCGTCGCTCCGTTTGCCGATTCGGCGGCGAGGCGGCGAGCGTCGAAAAG
CAGTCTCATTGCACCGAGTA

Downstream 100 bases:

>100_bases
GCGAGGCGGGCGATGGGCGGCGCGCGTGCCCGTCTTCGCCCGCGCTGAATGCGCCGGATTCCGCCTCCGTCAATCGTTTG
AATACCGAATGATTGGGATC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MMESKHLRYFLAIADTGSLTHAAIRLGVAQPALSHALTKMERELGVKLFDRSRRGATLTTAGEAIVDDIRLSLARMDAAA
RRASEIGAQRAGRLSIGFVSAALFDTLPAAVCALRAASPRVDLVMREMSNAEQVVALERGEIDIGILHTPVAIGGPVHEK
LIRRDPLIAVIPKSFPRQPDGSITLRELSRAGLIWFPHEQLPSIRAAILSAFRLAGHPIEVVQDANRTLTVIACVAAGCG
VSLLPSSIRTLTFEGVDFSPVADGQALPEFPLTAIWPKRSRRTLADRFAELLPRFQPS

Sequences:

>Translated_298_residues
MMESKHLRYFLAIADTGSLTHAAIRLGVAQPALSHALTKMERELGVKLFDRSRRGATLTTAGEAIVDDIRLSLARMDAAA
RRASEIGAQRAGRLSIGFVSAALFDTLPAAVCALRAASPRVDLVMREMSNAEQVVALERGEIDIGILHTPVAIGGPVHEK
LIRRDPLIAVIPKSFPRQPDGSITLRELSRAGLIWFPHEQLPSIRAAILSAFRLAGHPIEVVQDANRTLTVIACVAAGCG
VSLLPSSIRTLTFEGVDFSPVADGQALPEFPLTAIWPKRSRRTLADRFAELLPRFQPS
>Mature_298_residues
MMESKHLRYFLAIADTGSLTHAAIRLGVAQPALSHALTKMERELGVKLFDRSRRGATLTTAGEAIVDDIRLSLARMDAAA
RRASEIGAQRAGRLSIGFVSAALFDTLPAAVCALRAASPRVDLVMREMSNAEQVVALERGEIDIGILHTPVAIGGPVHEK
LIRRDPLIAVIPKSFPRQPDGSITLRELSRAGLIWFPHEQLPSIRAAILSAFRLAGHPIEVVQDANRTLTVIACVAAGCG
VSLLPSSIRTLTFEGVDFSPVADGQALPEFPLTAIWPKRSRRTLADRFAELLPRFQPS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=291, Percent_Identity=33.3333333333333, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788887, Length=278, Percent_Identity=29.8561151079137, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI2367136, Length=282, Percent_Identity=29.0780141843972, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788297, Length=250, Percent_Identity=32.4, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI157672245, Length=251, Percent_Identity=31.8725099601594, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788748, Length=242, Percent_Identity=23.5537190082645, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87081904, Length=142, Percent_Identity=33.0985915492958, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=24.0963855421687, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790399, Length=249, Percent_Identity=26.5060240963855, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789506, Length=281, Percent_Identity=23.4875444839858, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787601, Length=141, Percent_Identity=30.4964539007092, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790262, Length=251, Percent_Identity=24.7011952191235, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787806, Length=251, Percent_Identity=24.7011952191235, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790208, Length=264, Percent_Identity=24.2424242424242, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32242; Mature: 32242

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMESKHLRYFLAIADTGSLTHAAIRLGVAQPALSHALTKMERELGVKLFDRSRRGATLTT
CCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHCCCCCCCCEEEE
AGEAIVDDIRLSLARMDAAARRASEIGAQRAGRLSIGFVSAALFDTLPAAVCALRAASPR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCCH
VDLVMREMSNAEQVVALERGEIDIGILHTPVAIGGPVHEKLIRRDPLIAVIPKSFPRQPD
HHHHHHHHCCCHHEEEEECCCEEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCCCC
GSITLRELSRAGLIWFPHEQLPSIRAAILSAFRLAGHPIEVVQDANRTLTVIACVAAGCG
CCEEHHHHHCCCEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCC
VSLLPSSIRTLTFEGVDFSPVADGQALPEFPLTAIWPKRSRRTLADRFAELLPRFQPS
HHHCCCCHHEEEECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMESKHLRYFLAIADTGSLTHAAIRLGVAQPALSHALTKMERELGVKLFDRSRRGATLTT
CCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHCCCCCCCCEEEE
AGEAIVDDIRLSLARMDAAARRASEIGAQRAGRLSIGFVSAALFDTLPAAVCALRAASPR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCCH
VDLVMREMSNAEQVVALERGEIDIGILHTPVAIGGPVHEKLIRRDPLIAVIPKSFPRQPD
HHHHHHHHCCCHHEEEEECCCEEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCCCC
GSITLRELSRAGLIWFPHEQLPSIRAAILSAFRLAGHPIEVVQDANRTLTVIACVAAGCG
CCEEHHHHHCCCEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCC
VSLLPSSIRTLTFEGVDFSPVADGQALPEFPLTAIWPKRSRRTLADRFAELLPRFQPS
HHHCCCCHHEEEECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]