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The map label for this gene is rlmB [H]

Identifier: 83720095

GI number: 83720095

Start: 2871265

End: 2872008

Strand: Reverse

Name: rlmB [H]

Synonym: BTH_I2518

Alternate gene names: 83720095

Gene position: 2872008-2871265 (Counterclockwise)

Preceding gene: 83721027

Following gene: 83718870

Centisome position: 75.4

GC content: 69.89

Gene sequence:

>744_bases
ATGTCACGTCTGAAGGTTCTCTACGGTTTTCATGCAGTGACCGCGCGCATGCGGCACGATGCGTCGACGGTCGCGGAGGT
GTTCTACGATTCGACGCGTCGCGATCGCCGCATGCAGGATTTCCTGCATGCGGCGAAGGAAGCGGGCGTGCGCCTCATCG
CCGCCGACGAGACGCGGCTTTGGGGGCTTGCGCACACCGAGCGCCATCAGGGCGTCGTCGCGCGCGTCGAGGATCTGCCG
CTCGCGCAGAATCTCGCCGAGCTGCTCGACGGCGTCGCCGGGCCCGCGCTTCTGCTCGTGCTCGACGGCGTGACCGATCC
GCACAACCTCGGCGCATGCCTGCGCGTGGCCGATGCGGCGGGCGCGCACGCGATCATCGCGCCGCGCGACCGCGCGGCCG
GCCTGAATGCGACCGCGGCGAAGGTCGCGAGCGGCGCGGCCGACACGGTGCCGTACATCACGGTGACGAACCTCGCGCGT
GCGCTGCGCGAGCTGAAGGACGCGGGCGTGTGGGTGATCGGGACGTCGGACGACGCGACGGCGAGCCTTTACGACACGAA
GCTCGACGGCCCCGTCGCGCTCGTGATGGGCGCGGAAGGCGAGGGAATGCGGCGCCTGACGCGCGACACGTGCGACGAAG
TGATGTTCATCCCGATGGCGGGCAGTGTCGAGAGCCTGAACGTGTCGGTCGCGAGCGGCGTGTGTCTGTACGAGGCGGTG
CGCCAGCGGGGCGTGCGCAAGTAG

Upstream 100 bases:

>100_bases
CGAAACGCCGCGCGCCCCGCCAGGGCGCGCGGCGCTTTCCATTTTCCATGCGGCGCGTGCGATGCGCGGCCGCGCGTTTT
GATCGAAGGTTGTTCCAGCC

Downstream 100 bases:

>100_bases
GCGCGCGGCGCGTACGCTCAGCCCGCTGACGGCTTTGCGGGTGAGCGGCGCGCGCTGCTCGTTCGTGGCGCTCTATCGGC
TGCGCAGTGGCGGCGGGCGA

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSRLKVLYGFHAVTARMRHDASTVAEVFYDSTRRDRRMQDFLHAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLP
LAQNLAELLDGVAGPALLLVLDGVTDPHNLGACLRVADAAGAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLAR
ALRELKDAGVWVIGTSDDATASLYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGSVESLNVSVASGVCLYEAV
RQRGVRK

Sequences:

>Translated_247_residues
MSRLKVLYGFHAVTARMRHDASTVAEVFYDSTRRDRRMQDFLHAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLP
LAQNLAELLDGVAGPALLLVLDGVTDPHNLGACLRVADAAGAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLAR
ALRELKDAGVWVIGTSDDATASLYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGSVESLNVSVASGVCLYEAV
RQRGVRK
>Mature_246_residues
SRLKVLYGFHAVTARMRHDASTVAEVFYDSTRRDRRMQDFLHAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLPL
AQNLAELLDGVAGPALLLVLDGVTDPHNLGACLRVADAAGAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLARA
LRELKDAGVWVIGTSDDATASLYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGSVESLNVSVASGVCLYEAVR
QRGVRK

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=265, Percent_Identity=26.7924528301887, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=53.5269709543568, Blast_Score=253, Evalue=9e-69,
Organism=Escherichia coli, GI1788935, Length=244, Percent_Identity=28.6885245901639, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790083, Length=153, Percent_Identity=35.9477124183007, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17555908, Length=254, Percent_Identity=25.9842519685039, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=164, Percent_Identity=31.7073170731707, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26370; Mature: 26239

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLKVLYGFHAVTARMRHDASTVAEVFYDSTRRDRRMQDFLHAAKEAGVRLIAADETRL
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARVEDLPLAQNLAELLDGVAGPALLLVLDGVTDPHNLGACLRVADAA
EECCHHHHHCCCEEHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCC
GAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLARALRELKDAGVWVIGTSDDAT
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCC
ASLYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGSVESLNVSVASGVCLYEAV
EEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEHHHCCHHHHHHH
RQRGVRK
HHCCCCC
>Mature Secondary Structure 
SRLKVLYGFHAVTARMRHDASTVAEVFYDSTRRDRRMQDFLHAAKEAGVRLIAADETRL
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARVEDLPLAQNLAELLDGVAGPALLLVLDGVTDPHNLGACLRVADAA
EECCHHHHHCCCEEHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCC
GAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLARALRELKDAGVWVIGTSDDAT
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCC
ASLYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGSVESLNVSVASGVCLYEAV
EEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEHHHCCHHHHHHH
RQRGVRK
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]